AU2003238370B2 - Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same - Google Patents

Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same Download PDF

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Publication number
AU2003238370B2
AU2003238370B2 AU2003238370A AU2003238370A AU2003238370B2 AU 2003238370 B2 AU2003238370 B2 AU 2003238370B2 AU 2003238370 A AU2003238370 A AU 2003238370A AU 2003238370 A AU2003238370 A AU 2003238370A AU 2003238370 B2 AU2003238370 B2 AU 2003238370B2
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Prior art keywords
framework
single chain
antibody
sequence
sequences
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Expired
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AU2003238370A
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AU2003238370A1 (en
Inventor
Adrian Auf Der Maur
Alcide Barberis
Dominik Escher
Stefan Ewert
Kathrin Tissot
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Novartis AG
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Novartis AG
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Assigned to ESBATech AG, an Alcon Biomedical Research Unit LLC reassignment ESBATech AG, an Alcon Biomedical Research Unit LLC Amend patent request/document other than specification (104) Assignors: ESBATECH AG
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Publication of AU2003238370B2 publication Critical patent/AU2003238370B2/en
Assigned to ESBATECH, AN ALCON BIOMEDICAL RESEARCH UNIT LLC reassignment ESBATECH, AN ALCON BIOMEDICAL RESEARCH UNIT LLC Amend patent request/document other than specification (104) Assignors: ESBATech AG, an Alcon Biomedical Research Unit LLC
Priority to AU2010202634A priority Critical patent/AU2010202634B2/en
Priority to AU2012211497A priority patent/AU2012211497C1/en
Assigned to NOVARTIS AG reassignment NOVARTIS AG Request for Assignment Assignors: ESBATECH, AN ALCON BIOMEDICAL RESEARCH UNIT LLC
Anticipated expiration legal-status Critical
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/567—Framework region [FR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/80—Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/80—Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies
    • C07K2317/82—Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies functional in the cytoplasm, the inner aspect of the cell membrane, the nucleus or the mitochondria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2003238370A 2002-05-22 2003-05-21 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same Expired AU2003238370B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2010202634A AU2010202634B2 (en) 2002-05-22 2010-06-24 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same
AU2012211497A AU2012211497C1 (en) 2002-05-22 2012-08-01 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US38264902P 2002-05-22 2002-05-22
US60/382,649 2002-05-22
US43825603P 2003-01-03 2003-01-03
US60/438,256 2003-01-03
PCT/EP2003/005324 WO2003097697A2 (en) 2002-05-22 2003-05-21 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2010202634A Division AU2010202634B2 (en) 2002-05-22 2010-06-24 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same

Publications (2)

Publication Number Publication Date
AU2003238370A1 AU2003238370A1 (en) 2003-12-02
AU2003238370B2 true AU2003238370B2 (en) 2010-04-29

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AU2003238370A Expired AU2003238370B2 (en) 2002-05-22 2003-05-21 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same
AU2010202634A Expired AU2010202634B2 (en) 2002-05-22 2010-06-24 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same

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AU2010202634A Expired AU2010202634B2 (en) 2002-05-22 2010-06-24 Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same

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US (5) US8853362B2 (enExample)
EP (5) EP3656787A1 (enExample)
JP (10) JP2006508638A (enExample)
CN (4) CN105175535A (enExample)
AU (2) AU2003238370B2 (enExample)
CA (2) CA2867542C (enExample)
CY (1) CY1114225T1 (enExample)
DK (2) DK2332989T3 (enExample)
ES (3) ES2656427T3 (enExample)
HU (1) HUE048922T2 (enExample)
NZ (1) NZ536412A (enExample)
PT (2) PT2332989E (enExample)
SI (2) SI2332989T1 (enExample)
WO (1) WO2003097697A2 (enExample)

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HK1217712A1 (en) 2017-01-20
EP1506236B1 (en) 2013-01-23
JP2020171286A (ja) 2020-10-22
JP2010187678A (ja) 2010-09-02
US20170107272A1 (en) 2017-04-20
SI2947095T1 (sl) 2020-04-30
US9518108B2 (en) 2016-12-13
DK1506236T3 (da) 2013-03-04
AU2010202634B2 (en) 2012-08-23
EP3656787A1 (en) 2020-05-27
JP2015214583A (ja) 2015-12-03
CN1662556A (zh) 2005-08-31
CN103739706B (zh) 2015-11-18
ES2537104T3 (es) 2015-06-02
NZ536412A (en) 2008-10-31
PT2332989E (pt) 2015-08-05
EP1506236A2 (en) 2005-02-16
AU2003238370A1 (en) 2003-12-02
CA2483285C (en) 2015-01-27
HK1071378A1 (en) 2005-07-15
EP2314622B1 (en) 2017-10-18
JP2013256533A (ja) 2013-12-26
US10570190B2 (en) 2020-02-25
US20150057191A1 (en) 2015-02-26
EP2314622A1 (en) 2011-04-27
CA2483285A1 (en) 2003-11-27
JP2012228272A (ja) 2012-11-22
JP6261136B2 (ja) 2018-01-17
CA2867542C (en) 2020-04-14
CY1114225T1 (el) 2016-08-31
WO2003097697A2 (en) 2003-11-27
EP2332989B1 (en) 2015-04-01
US20190119361A1 (en) 2019-04-25
US20200165322A1 (en) 2020-05-28
EP2332989A1 (en) 2011-06-15
CN102093477B (zh) 2014-02-26
JP2006508638A (ja) 2006-03-16
CN102093477A (zh) 2011-06-15
EP2947095B1 (en) 2019-10-02
WO2003097697A3 (en) 2004-12-09
CN1662556B (zh) 2013-05-01
JP2015227372A (ja) 2015-12-17
US10125186B2 (en) 2018-11-13
JP6918033B2 (ja) 2021-08-11
AU2010202634A1 (en) 2010-07-15
US8853362B2 (en) 2014-10-07
JP2019058191A (ja) 2019-04-18
CN103739706A (zh) 2014-04-23
CN105175535A (zh) 2015-12-23
EP2947095A1 (en) 2015-11-25
DK2332989T3 (en) 2015-05-11
JP2017201990A (ja) 2017-11-16
CA2867542A1 (en) 2003-11-27
ES2656427T3 (es) 2018-02-27
PT1506236E (pt) 2013-03-27
ES2399617T3 (es) 2013-04-02
JP6075836B2 (ja) 2017-02-08
SI2332989T1 (sl) 2015-06-30
HUE048922T2 (hu) 2020-09-28
JP6100544B2 (ja) 2017-03-22
US20060035320A1 (en) 2006-02-16
JP2013078343A (ja) 2013-05-02
HK1152320A1 (en) 2012-02-24

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