CA2457930C - Procede et appareil d'epreuve diagnostique - Google Patents

Procede et appareil d'epreuve diagnostique Download PDF

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Publication number
CA2457930C
CA2457930C CA2457930A CA2457930A CA2457930C CA 2457930 C CA2457930 C CA 2457930C CA 2457930 A CA2457930 A CA 2457930A CA 2457930 A CA2457930 A CA 2457930A CA 2457930 C CA2457930 C CA 2457930C
Authority
CA
Canada
Prior art keywords
membrane
vessel
sample
analyte
capture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2457930A
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English (en)
Other versions
CA2457930A1 (fr
Inventor
Andrew John Sloane
Robert Alan Cole
William Samuel Hunter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Proteome Systems Ltd
Original Assignee
Proteome Systems Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AUPR7144A external-priority patent/AUPR714401A0/en
Priority claimed from AU2002950212A external-priority patent/AU2002950212A0/en
Application filed by Proteome Systems Ltd filed Critical Proteome Systems Ltd
Priority claimed from PCT/AU2002/001119 external-priority patent/WO2003016902A1/fr
Publication of CA2457930A1 publication Critical patent/CA2457930A1/fr
Application granted granted Critical
Publication of CA2457930C publication Critical patent/CA2457930C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306Solid-phase reaction mechanisms
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0642Filling fluids into wells by specific techniques
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0825Test strips
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/16Surface properties and coatings
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406Moving fluids with specific forces or mechanical means specific forces capillary forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/06Valves, specific forms thereof
    • B01L2400/0633Valves, specific forms thereof with moving parts
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/81Packaged device or kit
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/973Simultaneous determination of more than one analyte
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00Chemistry: analytical and immunological testing
    • Y10S436/807Apparatus included in process claim, e.g. physical support structures
    • Y10S436/81Tube, bottle, or dipstick

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne une méthode et un appareil destinés à être utilisés dans un processus de dosage à écoulement traversant. La méthode est caractérisée par une <= pré-étape d'incubation >= dans laquelle l'échantillon à analyser (dans lequel on cherche typiquement à déterminer la présence d'une protéine particulière) et un analyte de détection (typiquement un anticorps lié à de l'or colloïdal ou une étiquette fluorescente) connu pour se lier à la protéine particulière, peuvent se lier ensemble pendant une durée voulue. Cette pré-étape d'incubation se produit avant que le mélange de l'échantillon et de l'analyte de détection ne viennent en contact avec un analyte de capture lié à une membrane. Le fait de prévoir l'étape de pré-incubation a pour effet à la fois d'améliorer la sensibilité du dosage et de réduire le volume d'échantillon nécessaire à un dosage. L'invention concerne également un appareil destiné à mettre en oeuvre la méthode, définissant une chambre de pré-incubation destinée à recevoir l'échantillon ainsi que l'analyte de détection et présentant une base définie par une membrane ainsi qu'une seconde membrane à laquelle l'analyte de capture est lié. Dans une version, la chambre de pré-incubation est portée au-dessus de la seconde membrane, dans une position permettant de la pousser en contact avec la membrane portant l'analyte de capture, permettant ainsi le transfert de fluide depuis la chambre d'incubation à travers la membrane de capture. Dans une autre version, la membrane, à la base de la chambre d'incubation, est hydrophobe et son côté inférieur vient au contact de la membrane de capture, et lorsqu'un agent humidifiant est appliqué au contenu de la chambre de pré-incubation, un transfert de liquide se produit.
CA2457930A 2001-08-20 2002-08-20 Procede et appareil d'epreuve diagnostique Expired - Fee Related CA2457930C (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
AUPR7144A AUPR714401A0 (en) 2001-08-20 2001-08-20 Method and apparatus for screening for immunoreactivity against disease
AUPR7144 2001-08-20
AUPR9451 2001-12-12
AUPR945101 2001-12-12
AU2002950212 2002-07-11
AU2002950212A AU2002950212A0 (en) 2002-07-11 2002-07-11 Diagnostic testing process
PCT/AU2002/001119 WO2003016902A1 (fr) 2001-08-20 2002-08-20 Procede et appareil d'epreuve diagnostique

Publications (2)

Publication Number Publication Date
CA2457930A1 CA2457930A1 (fr) 2003-02-27
CA2457930C true CA2457930C (fr) 2012-06-19

Family

ID=32180066

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2457930A Expired - Fee Related CA2457930C (fr) 2001-08-20 2002-08-20 Procede et appareil d'epreuve diagnostique

Country Status (6)

Country Link
US (2) US7205159B2 (fr)
EP (1) EP1419387B1 (fr)
JP (1) JP4264348B2 (fr)
AT (1) ATE539816T1 (fr)
AU (1) AU2002331408B2 (fr)
CA (1) CA2457930C (fr)

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Also Published As

Publication number Publication date
EP1419387B1 (fr) 2012-01-04
EP1419387A4 (fr) 2005-12-14
US20050124077A1 (en) 2005-06-09
JP4264348B2 (ja) 2009-05-13
AU2002331408B2 (en) 2008-05-08
US20070190667A1 (en) 2007-08-16
JP2004538488A (ja) 2004-12-24
ATE539816T1 (de) 2012-01-15
US7205159B2 (en) 2007-04-17
EP1419387A1 (fr) 2004-05-19
CA2457930A1 (fr) 2003-02-27

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