CA2426121A1 - Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis - Google Patents

Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis Download PDF

Info

Publication number
CA2426121A1
CA2426121A1 CA002426121A CA2426121A CA2426121A1 CA 2426121 A1 CA2426121 A1 CA 2426121A1 CA 002426121 A CA002426121 A CA 002426121A CA 2426121 A CA2426121 A CA 2426121A CA 2426121 A1 CA2426121 A1 CA 2426121A1
Authority
CA
Canada
Prior art keywords
dye
compound
labelled
polynucleotide
energy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002426121A
Other languages
English (en)
French (fr)
Inventor
Linda G. Lee
Meng C. Taing
Barnett B. Rosenblum
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Publication of CA2426121A1 publication Critical patent/CA2426121A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/06—Hydroxy derivatives of triarylmethanes in which at least one OH group is bound to an aryl nucleus and their ethers or esters
    • C09B11/08—Phthaleins; Phenolphthaleins; Fluorescein
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/10—Amino derivatives of triarylmethanes
    • C09B11/24—Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Pyrane Compounds (AREA)
CA002426121A 2000-11-01 2001-10-30 Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis Abandoned CA2426121A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/704,966 US6448407B1 (en) 2000-11-01 2000-11-01 Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
US09/704,966 2000-11-01
PCT/US2001/048654 WO2002036832A2 (en) 2000-11-01 2001-10-30 Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis

Publications (1)

Publication Number Publication Date
CA2426121A1 true CA2426121A1 (en) 2002-05-10

Family

ID=24831567

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002426121A Abandoned CA2426121A1 (en) 2000-11-01 2001-10-30 Atropisomers of asymmetric xanthene fluorescent dyes and methods of dna sequencing and fragment analysis

Country Status (6)

Country Link
US (5) US6448407B1 (https=)
EP (1) EP1330550A2 (https=)
JP (1) JP2004532805A (https=)
AU (1) AU2002230914A1 (https=)
CA (1) CA2426121A1 (https=)
WO (1) WO2002036832A2 (https=)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6372907B1 (en) * 1999-11-03 2002-04-16 Apptera Corporation Water-soluble rhodamine dye peptide conjugates
US6727356B1 (en) * 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US20040081959A9 (en) * 1999-12-08 2004-04-29 Epoch Biosciences, Inc. Fluorescent quenching detection reagents and methods
US6448407B1 (en) * 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
WO2002048404A2 (en) * 2000-12-14 2002-06-20 Gen-Probe Incorporated Method and kit for enhancing the association rates of polynucleotides
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
WO2004003510A2 (en) * 2002-07-01 2004-01-08 Guava Technologies, Inc. Fluorescent dyes, energy transfer couples and methods
US7109316B2 (en) * 2002-08-23 2006-09-19 Ce Healthcare Bio-Sciences Corp. Oligonucleotide tagged nucleoside triphosphates (OTNTPs) for genetic analysis
CA2498320A1 (en) * 2002-09-20 2004-04-01 Integrated Dna Technologies, Inc. Anthraquinone quencher dyes, their methods of preparation and use
EP1606419A1 (en) 2003-03-18 2005-12-21 Quantum Genetics Ireland Limited Systems and methods for improving protein and milk production of dairy herds
US20050095610A1 (en) * 2003-04-11 2005-05-05 Kuo Sophia S. Method of generating size standard nucleic acids
US20040241732A1 (en) * 2003-04-11 2004-12-02 Chu-An Chang Method of generating long nucleic acid molecules of defined sequence
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
WO2005112544A2 (en) 2004-02-19 2005-12-01 The Governors Of The University Of Alberta Leptin promoter polymorphisms and uses thereof
US7635562B2 (en) 2004-05-25 2009-12-22 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7767834B2 (en) * 2004-08-13 2010-08-03 Elitech Holding B.V. Phosphonylated fluorescent dyes and conjugates
JP5214967B2 (ja) * 2004-08-13 2013-06-19 エポック バイオサイエンシズ インコーポレーティッド ホスホン酸蛍光色素および複合体
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
JP2008545659A (ja) 2005-05-20 2008-12-18 インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド オリゴヌクレオチドを標識する化合物及び方法
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
USRE49362E1 (en) 2006-05-18 2023-01-10 Illumina Cambridge Limited Dye compounds and the use of their labelled conjugates
US8350064B2 (en) 2006-07-21 2013-01-08 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Fluorescent xanthenes and white light fluorophores
US8426127B2 (en) 2006-12-19 2013-04-23 Riken Fluorescence-producing molecule
US8698031B2 (en) * 2007-04-17 2014-04-15 Dynamic Connections, Llc Separation and manipulation of a chiral object
US20110263041A1 (en) 2007-07-31 2011-10-27 Kazuhiko Nakatani Composition for measuring the binding affinity between nucleic acid and test substance, and use thereof
ES2548575T3 (es) 2009-09-18 2015-10-19 Provectus Pharmatech, Inc. Procedimiento para la síntesis de 4,5,6,7-tetracloro-3',6'-dihidroxi-2',4',5',7'-tetrayodo-3H-espiro[isobenzofuran-1,9'-xanten]-3-ona (Rosa de Bengala) y xantenos relacionados
US9273022B2 (en) 2009-09-18 2016-03-01 Provectus Pharmaceuticals, Inc. Process for the synthesis of 4,5,6,7-tetrachloro-3′,6′-dihydroxy-2′, 4′, 5′7′-tetraiodo-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one (Rose Bengal) and related xanthenes
EP2553123B1 (en) 2010-03-26 2016-08-24 Integrated DNA Technologies, Inc. Methods for enhancing nucleic acid hybridization
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
AU2011299233B2 (en) 2010-09-07 2016-09-15 Integrated Dna Technologies, Inc. Modifications for antisense compounds
CN104470991A (zh) * 2012-05-30 2015-03-25 生命科技公司 荧光pH敏感性染料和其使用方法
TWI468469B (zh) 2012-06-22 2015-01-11 財團法人工業技術研究院 染料與標記生物材料的方法
US9540699B2 (en) 2013-03-13 2017-01-10 William Beaumont Hospital Methods of diagnosing increased risk of developing MRSA
EP3019559A4 (en) * 2013-08-22 2017-04-05 Sony Corporation Water soluble fluorescent or colored dyes and methods for their use
KR101829159B1 (ko) 2014-01-16 2018-02-13 소니 주식회사 수용성 형광 또는 유색 염료
US9840340B2 (en) * 2014-12-09 2017-12-12 Dan O'Toole Drone docking station and delivery system
US11827661B2 (en) 2015-02-26 2023-11-28 Sony Group Corporation Water soluble fluorescent or colored dyes comprising conjugating groups
KR102646956B1 (ko) 2015-02-26 2024-03-14 소니그룹주식회사 페닐에티닐나프탈렌 염료 및 이의 사용 방법
CN104910229B (zh) * 2015-04-30 2019-11-12 赛纳生物科技(北京)有限公司 多聚磷酸末端荧光标记核苷酸及其应用
CN107709470B (zh) 2015-05-11 2021-01-29 索尼公司 超亮二聚或多聚染料
CN109415574B (zh) 2016-04-01 2021-05-28 索尼公司 具有刚性间隔基团的超亮二聚体或聚合物染料
AU2017240154B2 (en) 2016-04-01 2021-08-12 Sony Group Corporation Ultra bright dimeric or polymeric dyes
WO2017177065A2 (en) 2016-04-06 2017-10-12 Sony Corporation Ultra bright dimeric or polymeric dyes with spacing linker groups
JP7527537B2 (ja) 2016-05-10 2024-08-05 ソニーグループ株式会社 ペプチド骨格を有する超明色ポリマー染料
EP3455299B1 (en) 2016-05-10 2024-01-17 Sony Group Corporation Compositions comprising a polymeric dye and a cyclodextrin and uses thereof
EP3455300A1 (en) 2016-05-11 2019-03-20 Sony Corporation Ultra bright dimeric or polymeric dyes
EP3464477A1 (en) 2016-06-06 2019-04-10 Sony Corporation Ionic polymers comprising fluorescent or colored reporter groups
JP7312929B2 (ja) 2016-07-29 2023-07-24 ソニーグループ株式会社 超明色二量体またはポリマー色素およびその調製のための方法
CN107417670B (zh) * 2017-07-08 2019-06-04 常州大学 一种基于胸腺嘧啶的近红外方酸菁染料探针及其制备方法和应用
WO2019071153A1 (en) 2017-10-05 2019-04-11 Sony Corporation PROGRAMMABLE DENDRITIC MEDICINES
KR20260011205A (ko) 2017-10-05 2026-01-22 소니그룹주식회사 프로그램가능한 중합체성 약물
CN111836645A (zh) 2017-11-16 2020-10-27 索尼公司 可编程的聚合药物
US12539334B2 (en) 2018-01-12 2026-02-03 Sony Group Corporation Phosphoalkyl polymers comprising biologically active agents
EP3737417B1 (en) 2018-01-12 2025-08-27 Sony Group Corporation Phosphoalkyl ribose polymers comprising biologically active compounds
CN111565756A (zh) 2018-01-12 2020-08-21 索尼公司 包含生物活性化合物的具有刚性间隔基团的聚合物
CN108344722B (zh) * 2018-02-09 2020-08-21 赣南师范大学 一种近红外氧杂蒽类荧光染料在血吸虫成虫荧光标记中的应用
KR102742386B1 (ko) 2018-03-19 2024-12-16 소니그룹주식회사 형광 신호 향상을 위한 2가 금속의 사용
CN118480073A (zh) 2018-03-21 2024-08-13 索尼公司 具有连接体基团的聚合串联染料
JP7580689B2 (ja) 2018-06-27 2024-11-12 ソニーグループ株式会社 デオキシリボースを含むリンカー基を有するポリマー色素
KR20210032434A (ko) 2018-07-13 2021-03-24 소니 주식회사 유기인산염 단위를 포함하는 백본을 갖는 중합체성 염료
CN113195639A (zh) * 2018-12-20 2021-07-30 生命技术公司 非对称罗丹明染料和其在生物测定中的用途
SG11202106124UA (en) * 2018-12-20 2021-07-29 Life Technologies Corp Modified rhodamine dye and use thereof in biological assays
CN109741798B (zh) * 2018-12-25 2022-03-15 深圳晶泰科技有限公司 有机分子的立体异构全集自动化生成方法
WO2021062176A2 (en) 2019-09-26 2021-04-01 Sony Corporation Polymeric tandem dyes with linker groups
EP4034131A4 (en) * 2019-09-27 2023-10-18 Brentano Biotechnology Associates Diastereomeric linking reagents for nucleotide probes
EP4038081A1 (en) 2019-09-30 2022-08-10 Sony Group Corporation Nucleotide probes
CN111410624B (zh) * 2020-01-09 2022-05-06 江西科技师范大学 非对称萘-吡咯混联型二芳基乙烯化合物及其应用
CN115052882B (zh) * 2020-02-06 2024-05-24 豪夫迈·罗氏有限公司 减少模板穿入纳米孔的组合物
WO2021216072A1 (en) * 2020-04-23 2021-10-28 Hewlett-Packard Development Company, L.P. Three-dimensional printing
EP4185596A1 (en) 2020-07-23 2023-05-31 Life Technologies Corporation Energy transfer dye conjugates for use in biological assays
WO2022020731A2 (en) 2020-07-23 2022-01-27 Life Technologies Corporation Compositions, systems and methods for biological analysis involving energy transfer dye conjugates and analytes comprising the same
EP4256078A1 (en) 2020-12-07 2023-10-11 Sony Group Corporation Spacing linker group design for brightness enhancement in dimeric or polymeric dyes
CN115260235A (zh) * 2021-04-30 2022-11-01 苏州诺维康生物科技有限公司 一种荧光染料亚磷酰胺单体的制备方法
CN113563360A (zh) * 2021-08-16 2021-10-29 中国科学技术大学 一种两亲性荧光染料及其合成方法和应用
CN119462760A (zh) * 2024-10-15 2025-02-18 河北迪纳兴科生物科技有限公司 一种荧光标记的ddNTP及其合成方法和应用及一种DNA测序试剂盒及其制备方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4510251A (en) * 1982-11-08 1985-04-09 Abbott Laboratories Fluorescent polarization assay for ligands using aminomethylfluorescein derivatives as tracers
EP0201751A2 (en) 1985-05-08 1986-11-20 Abbott Laboratories 4'-aminomethyfluorescein derivatives for use in fluorescence polarization immunoassys
EP0232736A1 (en) 1986-02-06 1987-08-19 Abbott Laboratories Preparation of 4'-aminomethylfluorescein derivatives for use in fluorescence polarization immunoassays
US5188963A (en) 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5847162A (en) 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
US6448407B1 (en) * 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis

Also Published As

Publication number Publication date
US20040229235A1 (en) 2004-11-18
US6448407B1 (en) 2002-09-10
AU2002230914A1 (en) 2002-05-15
JP2004532805A (ja) 2004-10-28
US20060188915A1 (en) 2006-08-24
US7038063B2 (en) 2006-05-02
EP1330550A2 (en) 2003-07-30
US20070254298A1 (en) 2007-11-01
WO2002036832A2 (en) 2002-05-10
US20030055243A1 (en) 2003-03-20
US6649769B2 (en) 2003-11-18
WO2002036832A3 (en) 2002-08-01

Similar Documents

Publication Publication Date Title
US6448407B1 (en) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
CA2368495C (en) Electron-deficient nitrogen heterocycle-substituted fluorescein dyes
ES2329562T3 (es) Procedimiento para analizar la secuencia de un acido que utiliza colorantes de 4,7-dicloro-rodomina.
ES2225468T3 (es) Colorantes a base de cianina que modifican la movilidad.
US6583168B1 (en) Sulfonated diarylrhodamine dyes
AU2001259351B2 (en) Sulfonated (8,9)benzophenoxazine dyes and the use of their labelled conjugates
AU2001259351A1 (en) Sulfonated (8,9)benzophenoxazine dyes and the use of their labelled conjugates
AU2008200036A1 (en) Sulfonated diarylrhodamine dyes
WO2008039997A2 (en) Nucleobase conjugates with cationic backbone linkers

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued