AU2002230914A1 - Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis - Google Patents

Description

ATROPISOMERS OF ASYMMETRIC XANTHENE FLUORESCENT DYES AND METHODS OF DNA SEQUENCING AND FRAGMENT ANALYSIS

I. FIELD OF THE INVENTION

The invention relates to certain atropisomer forms of asymmetric xanthene fluorescent dyes and the field of nucleic acid sequencing and analysis with fluorescent dye-labelled reagents.

II. BACKGROUND OF THE INVENTION

Methods of analyzing fluorescent-labelled biomolecules after separating based on size- or charge are central to molecular biology. Examples of methods utilizing fluorescent- labelled nucleic acids include automated DNA sequencing, oligonucleotide probe methods, detection of polymerase-chain-reaction products, immunoassays, and the like, hi the case of multi-color automated DNA sequencing, labelled nucleic acid fragments of varying size are separated by electrophoresis, typically in a single electrophoresis lane, channel, or capillary. Employing these methods, automated four-color Sanger-type DNA sequencing has enabled entire genome characterization at the molecular level.

Stereochemical purity is of importance in the field of pharmaceuticals, where 12 of the 20 most prescribed drugs exhibit chirality (US 6,075,024). A case in point is provided by the L-form ofthe beta-adrenergic blocking agent, R(-) albuterol, which is known to be 100 times more potent than the D-enantiomer (US 5,760,090). Furthermore, optical purity is important since certain isomers may actually be deleterious rather than simply inert. Atropisomers are stereoisomeric conformations of a molecule whose interconversion is slow enough to allow separation and isolation under predetermined conditions (McGraw-Hill Dictionary of Chemical Terms, (1984), S. Parker, Ed., p. 36). The energy barrier to thermal racemization may be determined by the steric hindrance to free rotation of one or more bonds forming a chiral axis. Certain biaryl compounds exhibit atropisomerism where rotation around an intraannular bond lacking C₂ symmetry is restricted. The free energy barrier for enantiomerization is a measure ofthe stability of the intraannular bond with respect to rotation. Optical and thermal excitation can promote racemization, dependent on electronic and steric factors (Tetreau (1982) Nouv. Jour, de Chimie, 6:461-65).

Ortho-substituted biphenyl compounds may exhibit this type of conformational, rotational isomerism known as atropisomerism (Eliel, E. and Wilen, S. (1994) Stereochemistry of Organic Compounds, John Wiley & Sons, Inc., pp. 1142-55). Such biphenyls are enantiomeric, chiral atropisomers where the sp2-sp2 carbon-carbon, intraannular bond between the phenyl rings has a sufficiently high energy barrier to free rotation, and where substituents X ≠ Y and U ≠N render the molecule asymmetric. The steric interaction of X-U, X-N, and/or Y-N, Y-U is large enough to make the planar conformation an energy maximum. Two nonplanar, axially chiral enantiomers, shown below, then exist as atropisomers when their interconversion is slow enough such that they can be isolated free of each other. By one definition, atropisomerism is defined to exist where the isomers have a half-life tV of at least 1000 seconds, which is a free energy barrier of 22.3 kcal mol^"1 (93.3 kJ mol^"1) at 300K (Oki, M. (1983) "Recent Advances in Atropisomerism, " Topics in Stereochemistry, 14:1). Bold lines and dashed lines in the figures shown below indicate those moieties, or portions ofthe molecule, which are sterically restricted due to a rotational energy barrier. Bolded moieties exist orthogonally above the plane and dashed moieties exist orthogonally below the plane ofthe rest ofthe molecule.

III. SUMMARY

The present invention is directed towards atropisomerically-enriched and substantially pure atropisomers of asymmetric xanthene compounds as novel compositions. The invention also includes methods for isolation, labelling, and detecting labelled compositions.

In a first aspect, the invention includes substantially pure atropisomer compounds having the structure II:

wherein positions R¹, R⁴, R⁵, R¹¹, R¹³, R¹⁴, R¹⁷, R¹⁸, R¹⁹, R²⁰, Z¹, or Z² may be substituted with substituents. At least one substituent may be a linking moiety. One or more rings may be fused on the ring structure II. Another aspect ofthe invention includes energy-transfer dye compounds comprising a donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto; an acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response; and a linker for linking the donor dye and the acceptor dye; wherein at least one ofthe donor dye and acceptor dye is a substantially pure atropisomer of a xanthene compound.

Another aspect ofthe invention is labelled substrates, including nucleoside, nucleotides, polynucleotides, and polypeptides wherein the label is a substantially pure atropisomer of a xanthene compound or an energy-transfer dye comprising a substantially pure atropisomer of a xanthene compound.

Another aspect ofthe invention is labelling reagents, including phosphoramidite and active ester linking moieties of a substantially pure atropisomer of a xanthene compound, which form covalent attachments with substrates and methods of labelling substrates with the reagents. Another aspect ofthe invention is methods for forming a labelled substrate comprising the step of reacting a substrate with the linking moiety of a substantially pure atropisomer of a xanthene compound or an energy-transfer dye comprising a substantially pure atropisomer of a xanthene compound.

Another aspect ofthe invention is methods for separating atropisomers of xanthene compounds by forming diastereomers with substantially enantiomerically pure compounds, and separating the diastereomers. The diastereomers may be converted to substantially pure atropisomers of xanthene compounds.

Another aspect ofthe invention is methods for separating a mixture of labelled substrates wherein the labels are comprised of a substantially pure atropisomer of a xanthene compound or an energy-transfer dye comprising a substantially pure atropisomer of a xanthene compound. The labelled substrates may be primer extension polynucleotide fragments. The labelled substrates may be separated by electrophoresis, chromatography, or other separation technique. The mixture of labelled polynucleotides may be formed from a labelled primer or a labelled terminator. The labelled substrates may be detected by fluorescence detection.

Another aspect ofthe invention is methods of generating a labelled primer extension product by extending a primer-target hybrid with an enzymatically- incorporatable nucleotide. The primer or the nucleotide may be labelled with a substantially pure atropisomer of a xanthene compound or an energy-transfer dye comprising a substantially pure atropisomer of a xanthene compound.

Another aspect ofthe invention is methods of polynucleotide sequencing by forming a mixture of four classes of polynucleotides where each class is labelled at the 3' terminal nucleotide with a substantially pure atropisomer of a xanthene compound or an energy-transfer dye comprising a substantially pure atropisomer of a xanthene compound, and the labels are spectrally resolvable. The polynucleotides are separated by size.

Another aspect ofthe invention is methods of oligonucleotide ligation by annealing two probes to a target sequence and forming a phosphodiester bond between the 5' terminus of one probe and the 3' terminus ofthe other probe wherein one or both probes are labelled with a substantially pure atropisomer of a xanthene compound or an energy-transfer dye comprising a substantially pure atropisomer of a xanthene compound.

Another aspect ofthe invention is methods of amplification by annealing two or more primers to a target polynucleotide and extending the primers by a polymerase and a mixture of enzymatically-extendable nucleotides wherein at least one ofthe primers or one ofthe nucleotides is labelled with a substantially pure atropisomer of a xanthene compound or an energy-transfer dye comprising a substantially pure atropisomer of a xanthene compound.

Another aspect ofthe invention is kits of reagents including a substantially pure atropisomer of a xanthene compound or an energy-transfer dye comprising a substantially pure atropisomer of a xanthene compound.

IN. BRIEF DESCRIPTION OF THE DRAWINGS

Figure la shows the reaction of C-l aminomethyl, C-19 carboxy fluorescein and (-) Menthyl chloroformate to give menthyl carbamate diastereomers la and lb.

Figure lb shows reverse phase HPLC analysis of preparative HPLC fractions in the separation of menthyl carbamate diastereomers la and lb. Fractions #1 and #2 show pure la. Fraction #3 shows a mixture of la and lb. Fractions #4 and #5 show pure lb. Figure 2a shows the reactions of: (i) menthyl carbamate diastereomer la with sulfuric acid to hydrolyze the menthyl carbamate group to give atropisomer amine 2a, (ii) amidation of 2a with ethyl trifluoroacetate to give atropisomer trifluoroacetamide 3a, (iii) menthyl carbamate diastereomer lb with sulfuric acid to hydrolyze the menthyl carbamate group to give atropisomer amine 2b, and (iv) amidation of 2b with ethyl trifluoroacetate to give atropisomer trifluoroacetamide 3b.

Figure 2b shows an HPLC chromatogram ofthe racemic mixture of atropisomers 2a and 2b under chiral HPLC conditions.

Figure 2c shows an HPLC chromatogram ofthe purified atropisomer 2a under chiral

HPLC conditions. Figure 3 shows the reactions of: (i) atropisomer trifluoroacetamide 3a with N- i hydroxysuccinimide and DAE carbodiimide HCI salt to give atropisomeric

NHS ester 4a, and (ii) atropisomer trifluoroacetamide 3b with N- hydroxysuccinimide and DAE carbodiimide HCI salt to give atropisomeric

NHS ester 4b. Figure 4 shows the synthesis of 2-[(2-Fmoc- aminoethoxy)(hydroxyphosphoryl)oxy]acetic acid NHS 5. Figure 5 shows the synthesis of propargylphosphorylamino-ddATP 11. Figure 6 shows the synthesis of aminomethyl-FAM-propargylphosphorylamino-ddATP

15. Figure 7 shows the synthesis of tricyclic amine 20.

Figure 8 shows the synthesis of NHS-rhodamine dye 17.

Figure 9 shows the structure of energy-transfer ddATP terminator 25.

Figure 10 shows the synthesis of bis-trifluoroacetamide rhodamine NHS 27. Figure 11 shows the synthesis of aminomethylbenzamide-aminomethyl-FAM- propargylethoxyamino-ddTTP 32. Figure 12 shows the structure of energy-transfer ddTTP terminator 26. Figure 13a shows DNA sequencing of pGEM with phosphate-linker, energy-transfer terminator ddATP 25, fragment size 119-242 bp.

Figure 13b shows DNA sequencing of pGEM with phosphate-linker, energy-transfer terminator ddATP 25, fragment size 148-205 bp. Figure 13c shows DNA sequencing of pGEM with sulfonate-linker, energy-transfer terminator ddATP 33, fragment size 148-242 bp. Figure 13d shows DNA sequencing of pGEM with energy-transfer terminator ddGTP 34, fragment size 24-99 bp. Figure 14 shows the structure of sulfonate-linker, energy-transfer terminator ddATP 33. Figure 15 shows the structure of energy-transfer terminator ddGTP 34.

V. DETAILED DESCRIPTION OF EXEMPLARY EMBODIMENTS Reference will now be made in detail to certain embodiments ofthe invention, examples of which are illustrated in the accompanying drawings. While the invention will be described in conjunction with the illustrated embodiments, it will be understood that they are not intended to limit the invention to those embodiments. On the contrary, the invention is intended to cover all alternatives, modifications, and equivalents, which may be included within the scope ofthe claimed invention.

V.l DEFINITIONS

Stereochemical definitions and conventions used herein generally follow McGraw- Hill Dictionary of Chemical Terms (1984) S.P. Parker, Ed., McGraw-Hill Book Company, New York and Stereochemistry of Organic Compounds (1994); and Eliel, E. and Wilen, S., John Wiley & Sons, Inc., New York. Many organic compounds exist in optically active forms, i.e., they have the ability to rotate the plane of plane-polarized light. In describing an optically active compound, the prefixes D and L or R and S are used to denote the absolute configuration ofthe molecule about its chiral center(s). The prefixes d and 1 or (+) and (-) are employed to designate the sign of rotation of plane-polarized light by the compound, with (-) or 1 meaning that the compound is levorotatory. A compound prefixed with (+) or d is dextrorotatory. For a given chemical structure, these compounds, called stereoisomers, are identical except that they are mirror images of one another. A specific stereoisomer may also be referred to as an enantiomer, and a mixture of such isomers is often called an enantiomeric mixture. A 50:50 mixture of enantiomers is referred to as a racemic mixture or a racemate. The terms "racemic mixture" and "racemate" refer to an equimolar mixture of two enantiomeric species, devoid of optical activity.

The term "chiral" refers to molecules which have the property of non- superimposability ofthe mirror image partner, while the term "achiral" refers to molecules which are superimposable on their mirror image partner.

The term "stereoisomers" refers to compounds which have identical chemical constitution, but differ with regard to the arrangement ofthe atoms or groups in space.

"Diastereomer" refers to a stereoisomer with two or more centers of chirality and whose molecules are not mirror images of one another. Diastereomers have different physical properties, e.g. melting points, boiling points, spectral properties, and reactivities. Mixtures of diastereomers may separate under high resolution analytical procedures such as electrophoresis and chromatography.

"Enantiomers" refer to two stereoisomers of a compound which are non- superimposable mirror images of one another.

The term "atropisomer" refers to a stereoisomer resulting from restricted rotation about single bonds where the rotation barrier is high enough to permit isolation ofthe isomeric species. Atropisomers are enantiomers without a single asymmetric atom.

Atropisomers are conformational stereoisomers which occur when rotation about a single bond in the molecule is prevented, or greatly slowed, as a result of steric interactions with other parts ofthe molecule and the substituents at both ends ofthe single bond are unsymmetrical. The terms "substantially pure atropisomer" and "substantially free of its stereoisomer" mean that the composition contains at least 90% by weight of one atropisomer, and 10% by weight or less ofthe stereoisomeric atropisomer.

The term "atropisomerically enriched" means that the composition is a greater proportion or percentage of one ofthe atropisomers ofthe xanthene compound, in relation to the other atropisomer.

"Nucleobase" means a nitrogen-containing heterocychc moiety capable of forming Watson-Crick hydrogen bonds in pairing with a complementary nucleobase or nucleobase analog, e.g. a purine, a 7-deazapurine, or a pyrimidine. Typical nucleobases are the naturally occurring nucleobases adenine, guanine, cytosine, uracil, thymine, and analogs ofthe naturally occurring nucleobases, e.g. 7-deazaadenine, 7-deazaguanine, 7-deaza-8- azaguanine, 7-deaza-8-azaadenine (Kutyavin, U.S. Patent No. 5,912,340), inosine, nebularine, nitropyrrole, nitroindole, 2-aminopurine, 2,6-diaminopurine, hypoxanthine, pseudouridine, pseudocytosine, pseudoisocytosine, 5-propynylcytosine, isocytosine, isoguanine, 7-deazaguanine, 2-thiopyrimidine, 6-thioguanine, 4-thiothymine, 4- thiouracil, O^-methylguanine, N^-methyladenine, O -methylthymine, 5,6-dihydrothymine, 5,6- dihydrouracil, 4-methyl-indole, and ethenoadenine (Fasman (1989) Practical Handbook of Biochemistry and Molecular Biology, pp. 385-394, CRC Press, Boca Raton, FI).

"Νucleoside" means a compound consisting of a nucleobase linked to the C-l' carbon of a ribose sugar. The ribose may be substituted or unsubstituted. Substituted ribose sugars include, but are not limited to, those riboses in which one or more ofthe carbon atoms, e.g., the 3 '-carbon atom, is substituted with one or more ofthe same or different -R, -OR, -ΝRR or halogen groups, where each R is independently hydrogen, C C₆ alkyl or C₅-Cι₄ aryl. Riboses include ribose, 2'-deoxyribose, 2',3'-dideoxyribose, 3'-haloribose, 3'- fluororibose, 3'-chlororibose, 3'-alkylribose, e.g. 2'-O-methyl, 4' -α-anomeric nucleotides, l'-α-anomeric nucleotides, and 2'-4'-linked and other "locked", bicyclic sugar modifications (Imanishi WO 98/22489; Imanishi WO 98/39352; Wengel WO 99/14226). When the nucleobase is purine, e.g. A or G, the ribose sugar is attached to the Ν⁹-position ofthe nucleobase. When the nucleobase is pyrimidine, e.g. C, T or U, the pentose sugar is attached to the N¹ -position ofthe nucleobase (Kornberg and Baker, (1992) DNA Replication, 2^nd Ed., Freeman, San Francisco, CA).

"Nucleotide" means a phosphate ester of a nucleoside, as a monomer unit or within a nucleic acid. Nucleotides are sometimes denoted as "NTP", or "dNTP" and "ddNTP" to particularly point out the structural features ofthe ribose sugar. "Nucleotide 5'-triphosphate" refers to a nucleotide with a triphosphate ester group at the 5' position. The triphosphate ester group may include sulfur substitutions for the various oxygens, e.g. α-thio-nucleotide 5'-triphosphates.

As used herein, the terms "oligonucleotide" and "polynucleotide" are used interchangeably and mean single-stranded and double-stranded polymers of nucleotide monomers, including 2'-deoxyribonucleotides (DNA) and ribonucleotides (RNA) linked by internucleotide phosphodiester bond linkages, or internucleotide analogs, and associated counter ions, e.g., H^1", L*, trialkylammonium, Mg^2"1", Na⁺ and the like. A polynucleotide maybe composed entirely of deoxyribonucleotides, entirely of ribonucleotides, or chimeric mixtures thereof. Polynucleotides may be comprised of internucleotide, nucleobase and sugar analogs. Polynucleotides typically range in size from a few monomeric units, e.g. 5-40, when they are frequently referred to as oligonucleotides, to several thousands of monomeric nucleotide units. Unless denoted otherwise, whenever a polynucleotide sequence is represented, it will be understood that the nucleotides are in 5' to 3' order from left to right and that "A" denotes deoxyadenosine, "C" denotes deoxycytidine, "G" denotes deoxyguanosine, and "T" denotes thymidine, unless otherwise noted.

"Attachment site" means a site on a label or a substrate, such as an oligonucleotide, which is covalently attached to a linker. "Linker" means a chemical moiety comprising a covalent bond or a chain of atoms that covalently attaches a label to a polynucleotide, or one label to another.

"Linking moiety" means a chemically reactive group, substituent or moiety, e.g. a nucleophile or electrophile, capable of reacting with another molecule to form a covalent bond, or linkage. "Alkyl" means a saturated or unsaturated, branched, straight-chain, branched, or cyclic hydrocarbon radical derived by the removal of one hydrogen atom from a single carbon atom of a parent alkane, alkene, or alkyne. Typical alkyl groups consist of 1-12 saturated and/or unsaturated carbons, including, but not limited to, methyl, ethyl, propyl, butyl, and the like. "Alkoxy" means -OR where R is (Ci -C₆) alkyl.

"Alkyldiyl" means a saturated or unsaturated, branched, straight chain or cyclic hydrocarbon radical of 1-20 carbon atoms, and having two monovalent radical centers derived by the removal of two hydrogen atoms from the same or two different carbon atoms of a parent alkane, alkene or alkyne. Typical alkyldiyl radicals include, but are not limited to, 1,2-ethyldiyl, 1,3-propyldiyl, 1,4-butyldiyl, and the like.

"Aryl" means a monovalent aromatic hydrocarbon radical of 6-20 carbon atoms derived by the removal of one hydrogen atom from a single carbon atom of a parent aromatic ring system. Typical aryl groups include, but are not limited to, radicals derived from benzene, substituted benzene, naphthalene, anthracene, biphenyl, and the like. "Aryldiyl" means an unsaturated cyclic or polycyclic hydrocarbon radical of 6-20 carbon atoms having a conjugated resonance electron system and at least two monovalent radical centers derived by the removal of two hydrogen atoms from two different carbon atoms of a parent aryl compound. "Substituted alkyl", "substituted alkyldiyl", "substituted aryl" and "substituted aryldiyl" mean alkyl, alkyldiyl, aryl and aryldiyl respectively, in which one or more hydrogen atoms are each independently replaced with another substituent. Typical substituents include, but are not limited to, -X, -R, -O^", -OR, -SR, -S^", -NR₂, -NR₃, =NR, -CX₃, -CN, -OCN, -SCN, -NCO, -NCS, -NO, -NO₂, =N₂, -N₃, NC(O)R, -C(O)R, -

C(O)NRR -S(O)₂O^", -S(O)₂OH, -S(O)₂R, -OS(O)₂OR, -S(O)₂NR, -S(O)R, -OP(O)O₂RR - P(O)O₂RR -P(O)(O^")₂, -P(O)(OH)₂, -C(O)R, -C(O)X, -C(S)R, -C(O)OR, -C(O)O^", -C(S)OR, -C(O)SR, -C(S)SR, -C(O)NRR, -C(S)NRR, -C(NR)NRR, where each X is independently a halogen and each R is independently -H, alkyl, aryl, heterocycle, or linking group.

"Internucleotide analog" means a phosphate ester analog of an oligonucleotide such as: (i) alkylphosphonate, e.g. Cι-C₄ alkylphosphonate, especially methylphosphonate; (ii) phosphoramidate; (iii) alkylphosphotriester, e.g. Cι-C alkylphosphotriester; (iv) phosphorothioate; and (v) phosphorodithioate. Internucleotide analogs also include non- phosphate analogs wherein the sugar/phosphate subunit is replaced by an a non-phosphate containing backbone structure. One type of non-phosphate oligonucleotide analogs has an amide linkage, such as a 2-aminoethylglycine unit, commonly referred to as PNA (Nielsen (1991) "Sequence-selective recognition of DNA by strand displacement with a thymidine- substituted polyamide", Science 254:1497-1500). The terms "target sequence" and "target polynucleotide" mean a polynucleotide sequence that is the subject of hybridization with a complementary polynucleotide, e.g., a primer or probe. The sequence can be composed of DNA, RNA, an analog thereof, including combinations thereof.

The term "label", as used herein, means any moiety which can be attached to a substrate, e.g., an oligonucleotide, nucleotide or nucleotide 5'-triphosphate, and that functions to: (i) provide a detectable signal; (ii) interact with a second label to modify the detectable signal provided by the first or second label, e.g. FRET; (iii) stabilize hybridization, i.e. duplex formation; (iv) affect mobility, e.g. electrophoretic mobility or cell-permeability, by charge, hydrophobicity, shape, or other physical parameters, or (v) provide a capture moiety, e.g., affinity, antibody/antigen, or ionic complexation.

"Heterocycle" means a molecule with a ring system in which one or more ring atoms have been replaced with a heteroatom, e.g. nitrogen, oxygen, and sulfur.

"Electron-deficient nitrogen heterocycle" is a monovalent electron-deficient nitrogen heterocycle derived by the removal of one hydrogen atom from a single atom ofthe ring system to join the heterocycle as a substituent to the fluorescein dyes ofthe invention (Joule, Heterocychc Chemistry, 3rd Ed., Stanley Thornes Publisher, Ltd., Cheltenham, U.K. (1998); Acheson, R., An Introduction to the Chemistry of Heterocychc Compounds, 2nd Ed. friterscience Publishers, division of John Wiley & Sons, New York (1967)). "Substrate" is an entity to which dye compounds ofthe present invention are attached.

Substrates include, but are not limited to a (i) polynucleotide, (ii) nucleoside and nucleotide, (iii) polypeptide, (iv) carbohydrate, (v) ligand, and (vi) any analog ofthe preceding (i) to (v). "Enzymatically incorporatable" is a property of a nucleotide in which it is capable of being enzymatically incorporated onto the terminus, e.g. 3', of a nascent polynucleotide chain through the action of a polymerase enzyme.

"Terminator" means an enzymatically incorporatable nucleotide which prevents subsequent incorporations of nucleotides to the resulting polynucleotide chain and thereby halt polymerase extension. Typical terminators lack a 3 '-hydroxyl substituent and include 2',3'-dideoxyribose, 2',3'-didehydroribose, and 2',3'-dideoxy, 3'-haloribose, e.g. 3'-fluoro. Alternatively, a ribofuranose analog could be used, such as arabinose. Exemplary nucleotide terminators include 2',3'-dideoxy-β-D-ribofuranosyl, β-D-arabinofuranosyl, 3'- deoxy-β-D-arabinofuranosyl, 3'-amino-2',3'-dideoxy-β-D-ribofuranosyl, and 2',3'- dideoxy-3'-fluoro-β-D-ribofuranosyl (Chidgeavadze (1984) Nucleic Acids Res., 12: 1671- 1686; and Chidgeavadze (1985) FEB. Lett., 183: 275-278). Nucleotide terminators also include reversible nucleotide terminators (Metzker (1994) Nucleic Acids Res., 22(20): 4259).

"Enzymatically extendable" is a property of a nucleotide in which it is enzymatically incorporatable at the terminus of a polynucleotide and the resulting extended polynucleotide can undergo subsequent incorporations of nucleotides or nucleotide analogs.

V.2 ATROPISOMER COMPOUNDS

The compositions ofthe invention are asymmetric xanthene compounds that exist in stable atropisomeric forms. Aryl substituents can restrict rotation around the biannular bond between C-10 and C-15 (noted by an arrow) in the following structure I:

Asymmetric compounds ofthe invention include xanthene dyes characterized by the general structure II:

and include asymmetric fluorescent dye classes such as fluorescein (Z¹, Z² = O), rhodol (Z¹ = O, Z² = NR₂), and rhodamine (Z¹, Z² = NR₂). Where Z¹ or Z² is NR₂, R may independently be hydrogen, Cι-Cι₂ alkyl, phenyl, benzyl, aryl, heterocycle, or a linking moiety. Substituents R¹, R⁴, R⁵, R¹¹, R¹³, R¹⁴, R¹⁷, R¹⁸, R¹⁹, and R²⁰ may be independently fluorine, chlorine, Cι-C₈ alkyl, carboxylate, sulfate, sulfonate (-SO₃ ), alkylsulfonate

(-R-SO₃ ), aminomethyl (-CH NH₂), aminoalkyl, 4-dialkylaminopyridinium, hydroxymethyl (-CH₂OH), methoxy (-OCH₃), hydroxyalkyl (-ROH), thiomethyl (-CH₂SH), thioalkyl (-RSH), alkylsulfone (-SO₂R), arylthio (-SAr), arylsulfone (-SO₂Ar), sulfonamide (-SO₂NR₂), alkylsulfoxide (-SOR), arylsulfoxide (-SOAr), amino (-NH₂), ammonium (-NH₃ ⁺), amido (-CONR₂), nitrile (-CN), -Cs alkoxy (-OR), phenoxy, phenolic, tolyl, phenyl, aryl, benzyl, heterocycle, phosphonate, phosphate, quaternary amine, sulfate, polyethyleneoxy, and linking moiety.

The compounds ofthe invention include fused benzo rings where R¹³ and R¹⁴, or R⁴ and R , taken together form benzo, and where the fused benzo groups are substituted with substituents.

Substituents R¹, R⁴, R⁵, R¹¹, R¹³, R¹⁴, R¹⁷, R¹⁸, R¹⁹, and R²⁰ may also be independently an electron-deficient heterocycle, including 2-pyridyl, 3-pyridyl, 4-pyridyl, 2- quinolyl, 3-quinolyl, 4-quinolyl, 2-imidazole, 4-imidazole, 3-pyrazole, 4-pyrazole, pyridazine, pyrimidine, pyrazine, cinnoline, pthalazine, quinazoline, quinoxaline, 3-(l,2,4-N)- triazolyl, 5-(l,2,4-N)-triazolyl, 5-tetrazolyl, 4-(l-O, 3-N)-oxazole, 5-(l-O, 3-N)-oxazole, 4-(l- S, 3-N)-thiazole, 5-(l-S, 3-N)-thiazole, 2-benzoxazole, 2-benzothiazole, 4-(l,2,3-N)- benzotriazole, or benzimidazole.

Examples of asymmetric fluorescein dyes include the structures:

The compounds of the invention include atropisomeric, asymmetric rhodamines with ring structures formed by the Z¹ nitrogen, the Z^bonded carbon, and the R¹ -bonded carbon, to make a first ring structure having from 4 to 7 members. Optionally, the compounds may have a second ring structure formed by the Z² nitrogen, the Z -bonded carbon, and the R -bonded carbon, also having from 4 to 7 members. An example includes the structure Ila:

Asymmetry results where either: (1) R¹ ≠ R¹¹, R⁴ ≠ R¹⁴, R⁵ ≠ R¹³, or Z¹ ≠ Z², and (2) R ≠ R¹⁹, or R²⁰ ≠ X. In other words, both aryl substituents on the biannular sp2-sp2 bond are asymmetric and the compound lacks a C₂ axis of symmetry along the biannular bond axis. C₂ symmetry is defined by taking the biannular bond in I or II as the axis such that rotation of 180° around the axis, gives the same molecule. An example of a symmetric xanthene compound with C₂ symmetry is fluorescein (R¹, R⁴, R⁵, R¹¹, R¹³, R¹⁴,

17 1 Q 1 Q f) R , R , R , R = H; X = CO H). An example of an asymmetric xanthene compound without C₂ symmetry is C-l 1 aminomethyl, C-19 carboxyfluorescein (R¹, R⁴, R⁵, R¹³, R¹⁴, R¹⁷, R¹⁸, R²⁰ = H; R¹¹ = CH₂NH₂; R¹⁹, X = CO₂H). This compound is atropisomeric because the substituents adjacent to the C-l 0 to C-l 5 biannular bond are sufficiently bulky that rotation is hindered. The energy barrier to rotation is sufficiently high that stable, non-superimposable, mirror image atropisomeric forms 2a and 2b result, as shown:

Rotation around the biannular bond results in racemization and loss of atropisomerism. Typically, racemization ofthe asymmetric xanthene compounds occurs upon heating.

The compounds ofthe present invention can be prepared by any suitable method available in the art. Exemplary methods for preparing a variety of different asymmetric xanthene compounds can be found in the Example section below, and as discussed in greater detail below.

As a specific example, reference is made throughout the specification to Z¹ and Z² substituents. As this nomenclature corresponds to the illustrated structural formulae, which represent only one of several possible tautomeric forms (or resonance structures) of the compounds, it will be understood that these references are for convenience only, and that any such references are not intended to limit the scope ofthe compounds described herein.

Those of skill in the art will also recognize that the compounds ofthe invention may exist in many different protonation states, depending on, among other things, the pH of their environment. While the structural formulae provided herein depict the compounds in only one of several possible protonation states, it will be understood that these structures are illustrative only, and that the invention is not limited to any particular protonation state - any and all protonated forms ofthe compounds are intended to fall within the scope ofthe invention. The compounds ofthe invention may bear multiple positive or negative charges.

The net charge ofthe dyes ofthe invention may be either positive or negative. The counter ions associated with the dyes are typically dictated by the synthesis and/or isolation methods by which the compounds are obtained. Typical counter ions include, but are not limited to ammonium, sodium, potassium, lithium, halides, acetate, trifluoroacetate, etc., and mixtures thereof. It will be understood that the identity of any associated counter ion is not a critical feature ofthe invention, and that the invention encompasses the dyes in association with any type of counter ion. Moreover, as the compounds can exists in a variety of different forms, the invention is intended to encompass not only forms ofthe dyes that are in association with counter ions (e.g., dry salts), but also forms that are not in association with counter ions (e.g., aqueous or organic solutions).

Xanthene dyes have important applications as detectable fluorescent labels of nucleic acids (US Patent Nos. 5,188,934; 5,654,442; 5,885,778,; 6,096,723; 6,020,481;

5,863,727; 5,800996; 5,945,526; 5,847,162; 6,025,505; 6,008,379; 5,936,087; 6,015,719). Xanthene compounds containing an asymmetric biannular bond can exist in stable atropisomeric forms. Conjugates of atropisomeric xanthene compounds and chiral substrates, such as nucleotides, polynucleotides, polypeptides, and carbohydrates, form diastereomers. These diastereomeric conjugates can separate under certain conditions, such as electrophoresis, chromatography, and other methods. Separation of diastereomers can hinder detection by display of double peaks or bands, i.e. "peak doubling". Thus, atropisomerically enriched or purified forms of xanthene dyes are important as labels for methods based on separation and detection of analytes.

Asymmetric xanthene compounds can be conveniently synthesized from precursors (US Patent Nos. 5,188,934; 5,654,442; 5,885,778; 6,096,723; 6,020,481;

5,863,727; 5,800,996; 5,945,526; 5,847,162; 6,025,505; 6,008,379; 5,936,087; 6,015,719). An exemplary synthetic route starts by aminomethylation of C-l 9 carboxyfluorescein (Shipchandler (1987) Anal. Biochem. 162:89-101; U.S. Patent No. 4,510,251; EP 232736; EP110186) to give C-l (C-ll) aminomethyl, C-l 9 carboxyfluorescein. An atropisomer substantially free of its stereoisomer may be obtained by resolution ofthe mixture of stereoisomers of a xanthene compound using a method such as formation of diastereomers using optically active resolving agents ("Stereochemistry of Carbon Compounds," (1962) by E. L. Eliel, McGraw Hill; Lochmuller, C. H., (1975) J. Chromatogr., 113:(3) 283-302). Atropisomers of xanthene dyes can be separated and isolated, prior to, or after, derivatization to give reactive labelling reagents. Separation of the atropisomer xanthene compounds ofthe invention from the racemic mixture can be accomplished by any suitable method, including: (1) formation of ionic, diastereomeric salts with chiral compounds and separation by fractional crystallization or other methods, (2) formation of diastereomeric compounds with chiral derivatizing reagents, separation of the diastereomers, and conversion to the pure atropisomers, and (3) separation ofthe atropisomers directly under chiral conditions.

Under method (1), diastereomeric salts can be formed by reaction of enantiomerically pure chiral bases such as brucine, quinine, ephedrine, strychnine, α- methyl-β-phenylethylamine (amphetamine), and the like with asymmetric xanthene compounds bearing acidic functionality, such as carboxylic acid and sulfonic acid. The diastereomeric salts may be induced to separate by fractional crystallization or ionic chromatography. For separation ofthe optical isomers of amino compounds, addition of chiral carboxylic or sulfonic acids, such as camphorsulfonic acid, tartaric acid, mandelic acid, or lactic acid can result in formation ofthe diastereomeric salts.

Alternatively, by method (2), the substrate to be resolved is reacted with one enantiomer of a chiral compound to form a diastereomeric pair (Eliel, E. and Wilen, S. (1994) Stereochemistry of Organic Compounds, John Wiley & Sons, Inc., p. 322).

Diastereomeric compounds can be formed by reacting asymmetric xanthene compounds with enantiomerically pure chiral derivatizing reagents, such as menthyl derivatives, followed by separation ofthe diastereomers and hydrolysis to yield the free, enantiomerically enriched xanthene. A method of determining optical purity involves making chiral esters, such as a menthyl ester or Mosher ester, α-methoxy-α-

(trifluoromethyl)phenyl acetate (Jacob III. (1982) J. Org. Chem. 47:4165), ofthe racemic mixture, and analyzing the NMR spectrum for the presence ofthe two atropisomeric diastereomers. For example, C-l aminomethyl, C-l 9 carboxy fluorescein, an asymmetric xanthene compound useful for attaching to nucleotides, polynucleotides, and other fluorescent dyes was reacted with (-) menthyl chloroformate in the presence of base to form the diastereomeric mixture of menthyl carbamates la and lb (Example 1, Figure la). Stable diastereomers of atropisomeric xanthene compounds can be separated and isolated by normal- and reverse-phase cliromatography following methods for separation of atropisomeric naphthyl-isoquinolines (Hoye, T., WO 96/15111). Diastereomers la and lb were separated by preparative reverse-phase HPLC (Example 2, Figure lb).

By method (3), a racemic mixture of two asymmetric enantiomers can be separated by chromatography using a chiral stationary phase ("Chiral Liquid Chromatography" (1989) W. J. Lough, Ed. Chapman and Hall, New York; Okamoto, (1990) "Optical resolution of dihydropyridine enantiomers by High-performance liquid chromatography using phenylcarbamates of polysaccharides as a chiral stationary phase", J. of Chromatogr. 513:375-378). Enantiomeric atropisomers of xanthene compounds can be separated and isolated by chromatography on chiral stationary phase. A sample of racemic, C-l aminomethyl, C-l 9 carboxy fluorescein gave two peaks, resolving the atropisomeric stereoisomers, by HPLC analysis on a chiral adsorbent column (Figure 2b). When the atropisomers are separated, for example, by the chiral derivatization method (Example 1), preparative HPLC separation (Example 2) and hydrolysis ofthe chiral menthyl auxiliaries (Example 3, Figure 2a), the separated atropisomer 2a showed a single peak when analyzed by HPLC on the chiral adsorbent column (Figure 2c). Atropisomers can be distinguished by methods used to distinguish other chiral molecules with asymmetric carbon atoms, such as optical rotation and circular dichroism.

N.3 ENERGY-TRANSFER DYES

In another aspect, the present invention comprises energy-transfer dye compounds containing atropisomeric xanthene compounds such as those defined by structure U. Generally, the energy-transfer dyes ofthe present invention include a donor dye which absorbs light at a first wavelength and emits excitation energy in response, an acceptor dye which is capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response. The donor dye may be attached to the acceptor dye through a linker, the linker being effective to facilitate efficient energy transfer between the donor and acceptor dyes (Lee, "Energy-transfer dyes with enhanced fluorescence", U.S. Patent No. 5,800,996; Lee "Energy-transfer dyes with enhanced fluorescence", U.S. Patent No. 5,945,526; Mathies, " Fluorescent labels and their use in separations", U.S. Patent No. 5,654,419; Lee (1997) Nucleic Acids Res. 25:2816-22). Alternatively, the donor dye and the acceptor dye may be labelled at different attachment sites on the substrate. For example, an oligonucleotide may be labelled with a donor dye at the 5' terminus and an acceptor dye at the 3' terminus. A polypeptide may be labelled with a donor dye at the carboxyl terminus and an acceptor dye at an internal cysteine or lysine sidechain (Komoriya, "Compositions for the detection of proteases in biological samples and methods of use thereof, US Patent No. 5,605,809). In the energy-transfer dye ofthe invention, at least one ofthe donor or acceptor dyes which label a substrate is an atropisomeric xanthene compounds. Other dyes comprising the energy-transfer dye may be any fluorescent moiety which undergoes the energy transfer process with an atropisomeric xanthene compound, including a fluorescein, rhodol, and a rhodamine. Other dyes include classes of fluorescent dyes such as cyanine, phthalocyanine, squaraine, bodipy, benzophenoxazine, fluorescein, dibenzorhodamine, or rhodamine.

Energy-transfer dyes have advantages for use in the simultaneous detection of multiple labelled substrates in a mixture, such as DNA sequencing. A single donor dye can be used in a set of energy-transfer dyes so that each dye has strong absorption at a common wavelength. By then varying the acceptor dye in the energy-transfer set, the acceptor dyes can be spectrally resolved by their respective emission maxima. Energy- transfer dyes also provide a larger effective Stokes shift than non-energy-transfer dyes. The Stokes shift is the difference between the excitation maximum, the wavelength at which the donor dye maximally absorbs light, and the emission maximum, the wavelength at which the acceptor maximally emits light.

Generally the linker between the donor dye and acceptor dye has the structures:

wherein Z is selected from the group consisting of NH, S and O; R²¹ is a C₁-C₁₂ alkyl attached to the donor dye; R²² is a substituent selected from the group consisting of a C₁-C₁₂ alkyldiyl, a five and six membered ring having at least one unsaturated bond and a fused ring structure which is attached to the carbonyl carbon; and R²³ includes a functional group which attaches the linker to the acceptor dye. R²² may be a five or six membered ring such as cyclopentene, cyclohexene, cyclopentadiene, cyclohexadiene, furan, thiofuran, pyrrole, isopyrole, isoazole, pyrazole, isoimidazole, pyran, pyrone, benzene, pyridine, pyridazine, pyrimidine, pyrazine oxazine, indene, benzofuran, thionaphthene, indole and naphthalene. Specifically, the linker may have the structure:

where n ranges from 2 to 10.

Generally also, R²³ may have the structure:

wherein R²⁴ is a -C12 alkyl and

In one embodiment, the linker between the donor dye and acceptor dye includes a functional group which gives the linker some degree of structural rigidity, such as an alkene, diene, an alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure. The donor dye and the acceptor dye ofthe energy-transfer dye may be attached by linkers which have the exemplary structures:

DONOR wherein D is a donor dye, A is an acceptor dye and n is 1 or 2. The phenyl rings may be • substituted with groups such as sulfonate, phosphonate, and other charged groups.

The attachment sites ofthe linker between the donor dye and acceptor dye of an energy-transfer dye may be at any position where one or both ofthe donor dye and acceptor dye is a compound ofthe present invention. Exemplary attachment sites include R¹, R¹¹, R¹⁸, R¹⁹, Z¹ and Z². Examples of linkers and attachment sites are shown in terminator nucleotides 25, 26, 33, and 34 where the linkers attach at R¹ or R¹¹ ofthe donor fluorescein dye and at R¹⁸ or R¹⁹ ofthe acceptor rhodamine dye. An alternative embodiment is where the donor dye and the acceptor dye are attached by a linker through the R¹⁸ or R ⁹ sites, and either the donor dye or the acceptor dye is attached to a substrate through the R¹, R¹¹, Z¹ or Z² site. Another alternative embodiment is where the donor dye and the acceptor dye are attached by a linker through the R¹ , R¹¹ , Z¹ or Z² sites, and either the donor dye or the acceptor dye is attached to a substrate through the R¹⁸ or R¹⁹ site.

The energy-transfer dye compound is covalently attached to a substrate through a linker. The linker may be a bond, Cι-C₁₂ alkyldiyl or C₆-C ₀ aryldiyl. The linker may bear functional groups including amide, carbamate, urea, thiourea, phosphate, phosphonate, sulfonate, phosphorothioate, and the like. Preferred linkers include 1,2- ethyldiyl and 1,6-hexyldiyl. The attachment sites ofthe linker between the energy-transfer dye and the substrate may be at any position on the energy-transfer dye, where one or both ofthe donor dye and acceptor dye is a dye ofthe present invention. Where the substrate is a nucleoside or nucleotide, a preferred attachment site on the substrate is on the ■ nucleobase. If the nucleobase is a purine, the linker may be attached at the 8-position. If the nucleobase is a 7-deazapurine, the linker may be attached at the 7-position or 8- position. If the nucleobase is a pyrimidine, the linker may be attached at the 5-position. As examples, in terminator nucleotide examples 25 and 26, the energy-transfer dye is attached to the nucleobase at R¹⁹. Where the substrate is an oligonucleotide, preferred attachment sites include the 3' and 5' terminii. Other oligonucleotide attachment sites include the internucleotide phosphate, or phosphate-analog linkage, or at a position on the sugar, e.g. 2' or 4'. Where the substrate is a polypeptide (peptide or protein), preferred attachment sites include the amino and carboxyl termini, and lysine residue amino substituents.

N.4 METHODS OF LABELLING

The present invention comprises labelling reagents wherein atropisomeric xanthene compounds are in reactive form to react with substrates. In another aspect, the present invention comprises substrates labelled, i.e. conjugated, with the compounds ofthe invention, formula I. Substrates can be virtually any molecule or substance to which the dyes ofthe invention can be conjugated, including by way of example and not limitation, a polynucleotide, a nucleotide, a nucleoside, a polypeptide, a carbohydrate, a ligand, a substantially enantiomerically pure compound, a particle, a surface, a lipid, a solid support, organic and inorganic polymers, and combinations and assemblages thereof, such as chromosomes, nuclei, living cells (e.g., bacteria or other microorganisms, mammalian cells, tissues, etc.), and the like. A particle may include a nanoparticle, a microsphere, a bead, or a liposome. A surface may be glass or other non-porous planar material. The compounds ofthe invention are conjugated with the substrate via an optional linker by a variety of means, including hydrophobic attraction, ionic attraction, and covalent attachment.

Labelling typically results from mixing an appropriate reactive atropisomeric xanthene and a substrate to be conjugated in a suitable solvent, using methods well-known in the art (Hermanson, Bioconjugate Techniques, (1996) Academic Press, San Diego, CA. pp. 40-55, 643-71), followed by separation ofthe labelled substrate, conjugate, from any unconjugated starting materials or unwanted by-products. The conjugate can be stored dry or in solution for later use.

A racemic mixture of atropisomeric xanthenes may be separated to isolate substantially pure atropisomers at any intermediate stage in the synthesis ofthe labelling reagents, according to the aforementioned separation and isolation methods

(1), (2), and (3).

The atropisomeric xanthene may include a linking moiety at one ofthe substituent positions or covalent attachment ofthe dye to another molecule. A linking moiety is typically an electrophilic functional group, capable of forming a covalent bond by reacting with nucleophilic functionality on a substrate. Nucleophilic functionality may include, for example, alcohols, alkoxides, amines, hydroxylamines, and thiols. Alternatively, a linking moiety may include nucleophilic functionality that reacts with an electrophilic group on a substrate. Examples of linking moieties include azido, monosubstituted primary amine, disubstituted secondary amine, thiol, hydroxyl, halide, epoxide, N-hydroxysuccinimidyl ester, carboxyl, isothiocyanate, sulfonyl chloride, sulfonate ester, silyl halide, chlorotriazinyl, succinimidyl ester, pentafluorophenyl ester, maleimide, haloacetyl, epoxide, alkylhalide, allyl halide, aldehyde, ketone, acylazide, anhydride, iodoacetamide and an activated ester.

One linking moiety is N-hydroxysuccinimidyl ester (NHS) of a carboxyl group substituent ofthe atropisomeric xanthene compound (Figures 3, 6, 10, 11). The NHS ester form ofthe compound is a labelling reagent. The NHS ester ofthe dye may be preformed, isolated, purified, and/or characterized, or it may be formed in situ and reacted with a nucleophilic group of a substrate, such as an oligonucleotide, a nucleotide, a polypeptide, or the like (Brinkley, M. (1992) Bioconjugate Chem. 3:2-13). Typically, the carboxyl form ofthe dye is activated by reacting with some combination of: (1) a carbodiimide reagent, e.g. dicyclohexylcarbodiimide, diisopropylcarbodiimide, or a uranium reagent, e.g. TSTU (O-(N-Succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate, HBTU (O-benzotriazol- 1 -yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate), HATU (O-(7- azabenzotriazol-l-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate); (2) an activator, such as 1-hydroxybenzotriazole (HOBt); and (3) N-hydroxysuccinimide to give the NHS ester ofthe dye. A representative example of an NHS ester are structures 4a and 4b (Figure 3), 8 (Figure 4), and 13 (Figure 5). Functional groups on an atropisomeric xanthene compound may be protected prior to derivatization and reaction at other functional groups on the compound. For example, the amino group of atropisomers 2a and 2b were trifluoroacetylated to give 3a and 3b, separately (Figure 2a, Examples 5 and 6). The carboxyl groups of were then converted to the active ester, NHS with N-hydroxysuccinimide and a carbodiimide reagent, e.g. DAE to give 4a and 4b, separately (Figure 3, Examples 7 and 8).

In some cases, the atropisomeric xanthene compound and the substrate may be coupled by in situ activation ofthe compound and reaction with the substrate to form the atropisomeric xanthene-substrate conjugate in one step. Other activating and coupling reagents include TBTU (2-(lH-benzotriazo-l-yl)-l-l,3,3-tetramethyluronium hexafluorophosphate), TFFH (N,N',N",N'"-tetramethyluronium 2-fluoro- hexafluorophosphate), PyBOP (benzotriazole- 1 -yl-oxy-tris-pyrrolidino-phosphonium hexafluorophosphate, EEDQ (2-ethoxy- l-ethoxycarbonyl-l,2-dihydro-quinoline), DCC (dicyclohexylcarbodiimide); DIPCDI (diisopropylcarbodiimide), MSNT (l-(mesitylene-2- sulfonyl)-3-nitro-lH-l,2,4-triazole, and aryl sulfonyl halides, e.g. triisopropylbenzenesulfonyl chloride.

Another preferred reactive linking group is a phosphoramidite form of asymmetric xanthene compounds. Phosphoramidite dye reagents are particularly useful for the automated synthesis of oligonucleotides labelled with the dyes ofthe invention. Most conveniently, phosphoramidite dye reagents may be coupled to oligonucleotides bound to a solid support during the normal course of solid-phase synthesis. Oligonucleotides are commonly synthesized on solid supports by the phosphoramidite method (Caruthers, M. and Beaucage, S. "Phosphoramidite compounds and processes", U.S. Patent No. 4,415,732; Caruthers, M. and Matteucci, M. "Process for preparing polynucleotides", US Patent No. 4,458,066; Beaucage, S. and Iyer, R. (1992) "Advances in the synthesis of oligonucleotides by the phosphoramidite approach", Tetrahedron 48:2223-2311).

Phosphoramidite atropisomeric xanthene reagents can be nucleosidic or non- nucleosidic. Non-nucleosidic forms ofthe phosphoramidite reagents have the general formula III:

where DYE is a protected or unprotected form of atropisomer xanthene π, including energy-transfer dye; L is a linker; R and R taken separately are Cι-C₁₂ alkyl, C₄-C₁₀ aryl, and cycloalkyl containing up to 10 carbon atoms, or R³⁰ and R³¹ taken together with the phosphoramidite nitrogen atom form a saturated nitrogen heterocycle; and R³² is a phosphite ester protecting group which prevents unwanted extension ofthe oligonucleotide.

Generally, R is stable to oligonucleotide synthesis conditions yet is able to be removed from a synthetic oligonucleotide product with a reagent that does not adversely affect the integrity ofthe oligonucleotide or the dye. R³² may be: (i) methyl, (ii) 2-cyanoethyl; -

CH₂CH₂CN, or (iii) 2-(4-nitrophenyl)ethyl; -CH₂CH₂(p-NO₂Ph). Embodiments of phosphoramidite reagents include where: (i) R and R are each isopropyl, (ii) R and R taken together is morpholino, (iii) L is -C₁₂ alkyl, (iv) R is 2-cyanoethyl, and (v) DYE is attached at R¹⁸ or R¹⁹ by a linker. The linker, L, may alternatively be:

where n ranges from 1 to 10. An example of phosphoramidite reagent III has the structure:

Phosphoramidite dye reagents in effect labelling of a substrate with a single, substantially pure, atropisomeric xanthene ofthe invention. Where the substrate is an oligonucleotide, the dye will be attached at the 5' terminus ofthe oligonucleotide, as a consequence ofthe typical 3' to 5' direction of synthesis, or at the 3' terminus ofthe oligonucleotide when the 5' to 3' direction synthesis method is practiced (Wagner (1997) Nucleosides & Nucleotides 16:1657-60). Reagent in may be coupled to a polynucleotide which is bound to a solid support, e.g. through the 3' terminus. Other phosphoramidite dye reagents, nucleosidic and non-nucleosidic allow for labelling at other sites of an oligonucleotide, e.g. 3' terminus, nucleobase, internucleotide linkage, sugar. Labelling at the nucleobase, internucleotide linkage, and sugar sites allows for internal and multiple labelling with fluorescent dyes.

An atropisomeric xanthene compound ofthe invention may be converted to a non-nucleosidic, phosphoramidite labelling reagent by any known method of phosphitylation of nucleophilic functionality with trivalent phosphitylating reagents. For example, when the xanthene contains a carboxyl group, e.g. R¹⁹ = CO₂H, the carboxyl may be activated, e.g. to the NHS, and amidated with 6-amino-l-hexanol. The resulting hydroxyl may be phosphitylated with bis(diisopropylamino)cyanoethylphosphite or chloro-diisopropylamino- cyanoethylphosphine to give the phosphoramidite dye-labelling reagent (Theisen (1992) "Fluorescent dye phosphoramidite labelling of oligonucleotides", in Nucleic Acid Symposium Series No. 27, Oxford University Press, Oxford, pp. 99-100). Alternatively, the carboxyl group ofthe compound may be reduced to the hydroxyl, to be phosphitylated. The phosphoramidite reagent HI reacts with a hydroxyl group, e.g. 5' terminal OH of an oligonucleotide bound to a solid support, under mild acid activation, to form an internucleotide phosphite group which is then oxidized to an internucleotide phosphate group. In some instances, the xanthene compound may contain functional groups, e.g. Z¹ and Z² oxygens as in structure I, that require protection either during the synthesis ofthe phosphoramidite reagent or during its subsequent use to label molecules such as oligonucleotides. The protecting group(s) used will depend upon the nature ofthe functional groups, and will be apparent to those having skill in the art (Greene, T. and Wuts, P. Protective Groups in Organic Synthesis, 2nd Ed., John Wiley & Sons, New York, 1991). Generally, the protecting groups used should be stable under the acidic conditions (e.g. trichloroacetic acid, dichloroacetic acid) commonly employed in oligonucleotide synthesis to remove 5'-hydroxyl protecting groups (e.g., dimethoxytrityl) and labile under the basic conditions (ammonium hydroxide, aqueous methylamine) used to deprotect and/or cleave synthetic oligonucleotides from solid supports.

Polypeptides, antibodies, and other biopolymers comprised of amino acids and amino acid analogs may be covalently labelled by conjugation with the atropisomeric xanthene compounds ofthe invention. Typically, the compound is in electrophilic form, e.g. NHS reactive linking group, which reacts with a nucleophilic group ofthe peptide, e.g. amino terminus, or amino side chain of an amino acid such as lysine. Alternatively, the dye may be in nucleophilic form, e.g. amino- or thiol-reactive linking group, which may react with an electrophilic group ofthe peptide, e.g. NHS ofthe carboxyl terminus or carboxyl side chain of an amino acid. Labelled polypeptides may retain their specific binding and recognition properties in interacting with cell surface and intracellular components. The xanthene compound, acting as a dye, provides a detection element for localizing, visualizing, and quantitating the binding or recognition event. Polypeptides can also be labelled with two moieties, a fluorescent reporter and quencher, which together undergo fluorescence resonance energy transfer (FRET). The fluorescent reporter may be partially or significantly quenched by the quencher moiety in an intact polypeptide. Upon cleavage ofthe polypeptide by a peptidase or protease, a detectable increase in fluorescence maybe measured (Knight, C. (1995) "Fluorimetric Assays of Proteolytic Enzymes", Methods in Enzymology, Academic Press, 248:18-34).

V.4A LABELLED NUCLEOTIDES A preferred class of labelled substrates include conjugates of nucleosides and nucleotides that are labelled with the dyes ofthe invention. Such labelled nucleosides and nucleotides are particularly useful for labelling polynucleotides formed by enzymatic synthesis, e.g., labelled nucleotide 5'-triphosphates used in the context of PCR amplification, Sanger-type polynucleotide sequencing, and nick-translation reactions.

Nucleosides and nucleotides can be labelled at sites on the sugar or nucleobase moieties. Preferred nucleobase labelling sites include the 8-C of a purine nucleobase, the 7- C or 8-C of a 7-deazapurine nucleobase, and the 5-position of a pyrimidine nucleobase. Between a nucleoside or nucleotide and a dye, a linker may attach to an atropisomeric xanthene compound at any position.

The labelled nucleoside or nucleotide may be enzymatically incorporatable and enzymatically extendable. Nucleosides or nucleotides labelled with compounds ofthe invention may have formula IN:

where DYE is a protected or unprotected form of compounds I or II, including energy- transfer dye. B may be any nucleobase, e.g. uracil, thymine, cytosine, adenine, 7- deazaadenine, guanine, and 8-deazaguanosine. R²⁵ is H, monophosphate, diphosphate, triphosphate, thiophosphate, or phosphate ester analog. R²⁶ and R²⁷, when taken alone, are each independently H, HO, F and a phosphoramidite. Where R²⁶ or R²⁷ is phosphoramidite, R²⁵ is an acid-cleavable hydroxyl protecting group, e.g. dimethoxytrityl, which allows subsequent monomer coupling under automated synthesis conditions (Caruthers, "Phosphoramidite compounds and processes", U.S. Patent No. 4,415,732; Caruthers, "Process for preparing polynucleotides", US Patent No. 4,458,066; Beaucage, S. and Iyer, R. (1992) "Advances in the synthesis of oligonucleotides by the phosphoramidite approach", Tetrahedron 48:2223-2311). Where the labelled nucleoside or nucleotide is a terminator, R²⁶ and R²⁷ are selected to block polymerase-mediated template-directed polymerization. In terminator nucleotides, R²⁶ and R²⁷, when taken alone, are each independently H, F, and a moiety which blocks polymerase-mediated template-directed polymerization, or when taken together form 2'-3'-didehydroribose. In formula IV, when both R²⁶ and R²⁷ are hydroxyl, the resultant compounds are labelled ribonucleosides and ribonucleotides (NTP). When R²⁷ is hydrogen and R²⁶ is hydroxyl, the resultant compounds are labelled 2'-deoxyribonucleosides and nucleotides (dNTP). When R 26 and R²⁷ are each hydrogen, the resultant compounds are 2',3'-dideoxyribonucleosides and nucleotides (ddNTP). Labelled ddNTP find particular use as terminators in Sanger-type DNA sequencing methods utilizing fluorescent detection. Labelled 2'-deoxyribonucleoside-5'-triphosphates (dNTP) find particular use as reagents for labelling DNA polymerase extension products, e.g., in the polymerase chain reaction or nick-translation. Labelled ribonucleoside-5'-triphosphates (NTP) find particular use as reagents for labelling RNA polymerase extension products.

2',3^,-dehydro-ddNTP

Alkynylamino-linked compounds IV, where L includes an alkyndiyl group, are useful for conjugating atropisomeric xanthene compounds to nucleosides, nucleotides and analogs therein. Their synthesis is taught in EP 87305844.0 and Hobbs, (1989) J. Org.

Chem. 54:3420. The corresponding nucleoside mono-, di- and triphosphates are obtained by standard techniques (for example, the methods described in U.S. Patent Nos. 5,821,356; 5,770,716; 5,948,648; 6,096,875). Methods for synthesizing compounds IV with modified propargylethoxyamido linkers L can also be found in these patents. Additional synthesis procedures suitable for use in synthesizing compounds according to structural formula IV are described, for example, in Gibson (1987) Nucl. Acids Res. 15:6455-6467; Gebeyehu (1987) Nucl. Acids Res. 15:4513-4535; Haralambidis (1987) Nucl. Acids Res. 15:4856-4876; Nelson (1986) Nucleosides and Nucleotides. 5(3):233-241; Bergstrom (1989) J. Am. Chem. Soc. 111:374-375; U.S. Patent No. 4,855,225, U.S. Patent No. 5,231,191 and U.S. Patent No. 5,449,767, which are . incorporated herein by reference. Any of these methods can be routinely adapted or modified as necessary to synthesize the full range of labelled nucleosides, nucleotides, and analogs described herein.

One embodiment ofthe alkynyl linker L may be:

O — C≡≡C— CH₂- (OCH₂CH₂)_n— NH— C — wherein n is 0, 1 , or 2.

Energy-transfer dye pairs can be conjugated to a nucleotide 5 '-triphosphate by linking through a nucleobase amino group to: (i) an activated ester of a energy-transfer dye pair, or (ii) stepwise coupling to one dye, e.g. R -protected aminomethyl, R - carboxyl fluorescein, then coupling the unprotected R¹ ^aminomethyl to the second dye ofthe pair.

Linker reagents may be prepared by known synthetic methods. For example, phosphate linker reagent 5 is synthesized starting from the cyclic phosphoramidite 7. Phosphitylation of 7 with methyl glycolate 6 was followed by in situ oxidation to the pentavalent phosphate 8. Hydrolysis ofthe methyl ester, the trifluoroacetate group, and demethylation gave 9. Protection ofthe amino group with Fmoc gave 10 which was activated as the N-hydroxysuccinimide ester, linker reagent 5 (Figure 4, Example

9).

An alkynylamino-lihked nucleotide can be prepared by first coupling NHS linker reagent 5 with 7-deaza-7-propargylamino-ddATP 12 to give 13, followed by hydrolysis ofthe Fmoc group to give 11 (Figure 5, Example 10). The amino atropisomeric xanthene la is coupled with the N-Fmoc, NHS ester of p- aminomethylbenzoic acid (Example 11) and then activated as the NHS ester to give 14 (Figure 6). Reaction of 11 and 14 gave the atropisomeric xanthene ddATP compound 16. The Fmoc group was removed with ammonium hydroxide to give 15 (Figure 6, Example 12).

The NHS-rhodamine dye 17 was synthesized from bicyclic amine 18. Cyclization with l-bromo-3-chloropropane gave tricyclic ester 19, which was hydrolyzed to tricyclic amine 20 (Figure 7, Example 13). Friedel-Crafts acylation of 20 with anhydride 21 gave the ketone 22 which was reacted with another equivalent of 20 to give symmetric rhodamine isopropyl ester 23. The ester of 23 was cleaved and the carboxylic acid 24 was converted to NHS-rhodamine dye 17 (Figure 8, Example 13). The substantially pure atropisomer xanthene energy transfer ddATP terminator 25 was formed by coupling 15 with 17, followed by anion-exchange HPLC purification (Figure 9, Example 14).

Alternative synthetic routes to energy-transfer nucleotides and polynucleotides, with different convergent schemes may be practiced. The substrate, dye, and linker subunits, or synthons, may be assembled for coupling in any order. For example, the energy-transfer pair of donor dye and acceptor dye may be covalently attached through a linker and then coupled to the nucleotide or polynucleotide. Many different synthetic routes can be practiced which result in the labelling of nucleotides with the dyes ofthe invention. Reactive functionality, such as carboxylic acid, amino, hydroxyl groups, may require protection, utilizing the vast art of organic synthesis methodology.

Another rhodamine dye 28 was protected as the bis-trifluoroacetamide 29 and converted to the NHS compound 27 (Figure 10, Example 15). Propargylethoxyamino ddTTP 30 was coupled with atropisomer xanthene compound 14 to give Fmoc atropisomer xanthene ddTTP 31 which was hydrolyzed to 32 (Figure 11, Example 16). Reaction of 27 and 32 gave atropisomer, energy-transfer terminator ddTTP 26, purified by anion-exchange HPLC (Figure 12, Example 17).

V.4B LABELLED OLIGONUCLEOTIDES Oligonucleotides are commonly synthesized on solid supports by the phosphoramidite method (U.S. Patent Nos. 4,415,732; 4,973,679; 4,458,066; Beaucage, S. and Iyer, R. (1992) Tetrahedron 48:2223-2311) using commercially available phosphoramidite nucleosides, supports e.g. silica, controlled-pore-glass (U.S. Patent No. 4,458,066) and polystyrene (U.S. Patent Nos. 5,047,524 and 5,262,530) and automated synthesizers (Models 392, 394, 3948 DNA/RNA Synthesizers, Applied Biosystems). Another preferred class of labelled substrates include conjugates of oligonucleotides and the compounds ofthe invention. Such conjugates may find utility as DNA sequencing primers, PCR primers, oligonucleotide hybridization probes, oligonucleotide ligation probes, double-labelled 5'-exonuclease (TaqMan™) probes, and the like (Fung, U.S. Patent No. 4,757,141; Andrus, "Chemical methods for 5' non-isotopic labelling of PCR probes and primers" (1995) in PCR 2: A Practical Approach, Oxford University Press, Oxford, pp. 39-54; Hermanson, Bioconjugate Techniques, (1996) Academic Press, San Diego, CA. pp. 40-55, 643-71; Mullah (1998) "Efficient synthesis of double dye-labelled oligodeoxyribonucleotide probes and their application in a real time PCR assay", Nucl. Acids Res. 26:1026-1031). A labelled oligonucleotide may have formula V:

where the oligonucleotide comprises 2 to 100 nucleotides. DYE is a protected or unprotected form of compounds I or II, including energy-transfer dye. B is any nucleobase, e.g. uracil, thymine, cytosine, adenine, 7-deazaadenine, guanine, and 8- deazaguanosine. L is a linker. R²⁷ is H, OH, halide, azide, amine, Cι-C₆ aminoalkyl, Cι-C₆ alkyl, allyl, Cι-C₆ alkoxy, OCH₃, or OCH₂CH=CH . R²² is H, phosphate, internucleotide phosphodiester, or internucleotide analog. R²⁹ is H, phosphate, internucleotide phosphodiester, or internucleotide analog. In this embodiment, structure V, the nucleobase-labelled oligonucleotide may bear multiple dyes ofthe invention attached through the nucleobases. Nucleobase-labelled oligonucleotide V may be formed by: (i) enzymatic incorporation of enzymatically incorporatable nucleotide reagents IV where R²⁵ is triphosphate, by a DNA polymerase or ligase, and (ii) coupling of a nucleoside phosphoramidite reagent by automated synthesis. Whereas, nucleobase-labelled oligonucleotides V may be multiply labelled by incorporation of more than one incorporatable nucleotide IV, labelling with a dye label reagent such as III leads to singly 5'-labelled oligonucleotides, according to formula VI:

where X is O, NH, or S; R²⁷ is H, OH, halide, azide, amine, Cι-C₆ aminoalkyl, Cι-C₆ alkyl, allyl, Cι-C₆ alkoxy, OCH₃, or OCH₂CH=CH₂; R²⁸ is H, phosphate, internucleotide phosphodiester, or internucleotide analog; and L is Ci-C₁₂ alkyl, aryl, or polyethyleneoxy of up to 100 ethyleneoxy units. The linker L in formulas V or VI may be attached at any site on the atropisomeric xanthene compound ofthe invention, DYE, including R¹, R^π, R¹⁸, R¹⁹, Z¹ and Z² of structure I. hi a first method for labelling synthetic oligonucleotides, a nucleophilic functionality, e.g. a primary aliphatic amine, is introduced at a labelling attachment site on an oligonucleotide, e.g. a 5' terminus. After automated, solid-support synthesis is complete, the oligonucleotide is cleaved from the support and all protecting groups are removed. The nucleophile-oligonucleotide is reacted with an excess of a label reagent containing an electrophilic moiety, e.g. isothiocyanate or activated ester, e.g. N- hydroxysuccinimide (NHS), under homogeneous solution conditions (Hermanson, Bioconjugate Techniques, (1996) Academic Press, San Diego, CA. pp. 40-55, 643-71; Andrus, A. "Chemical methods for 5' non-isotopic labelling of PCR probes and primers" (1995) in PCR 2: A Practical Approach, Oxford University Press, Oxford, pp. 39-54). Labelled oligonucleotides VI may be formed by reacting a reactive linking group form , e.g. NHS, of a dye, with a 5'-aminoalkyl oligonucleotide.

In a second method, a label is directly incorporated into the oligonucleotide during or prior to automated synthesis, for example as a support reagent (Mullah, "Solid support reagents for the direct synthesis of 3'-labelled polynucleotides", U.S. patent 5,736,626; Nelson, "Multifunctional controlled pore glass reagent for solid phase oligonucleotide synthesis", U.S. Patent No. 5,141,813) or as a phosphoramidite reagent III. Certain fluorescent dyes and other labels have been functionalized as phosphoramidite reagents for 5' labelling (Theisen (1992) Nucleic Acid Symposium Series No. 27, Oxford University Press, Oxford, pp. 99-100).

Generally, if the labelled oligonucleotide is made by enzymatic synthesis, the following procedure may be used. A target DNA is denatured and an oligonucleotide primer is annealed to the template DNA. A mixture of enzymatically-incorporatable nucleotides or nucleotide analogs capable of supporting continuous template-directed enzymatic extension ofthe primed target (e.g., a mixture including dGTP, dATP, dCTP and dTTP or dUTP) is added to the primed target. At least a fraction ofthe nucleotides are labelled terminators IN, labelled with an atropisomer xanthene dye II. A polymerase enzyme is next added to the mixture under conditions where the polymerase enzyme is active. A labelled oligonucleotide is formed by the incorporation ofthe labelled nucleotides or terminators during polymerase-mediated strand synthesis. In an alternative enzymatic synthesis method, two primers are used instead of one: one complementary to the (+) strand ofthe target and another complementary to the (-) strand ofthe target, the polymerase is a thermostable polymerase and the reaction temperature is cycled between a denaturation temperature and an extension temperature, thereby exponentially synthesizing a labelled complement to the target sequence by PCR (Innis (1990) PCR Protocols, Eds., Academic Press).

In one preferred post-synthesis chemical labelling method an oligonucleotide is labelled as follows. An NHS form of a dye according to structure I is dissolved or suspended in DMSO and added in excess (5-20 equivalents) to a 5'-aminohexyl oligonucleotide in 0.25 M bicarbonate/carbonate buffer at about pH 9 and allowed to react for 6 hours, e.g., U.S. Patent No. 4,757,141. The atropisomer xanthene labelled oligonucleotide can be separated from unreacted dye by passage through a size-exclusion chromatography column eluting with buffer, e.g., 0.1 molar triethylamine acetate (TEAA). The fraction containing the crude labelled oligonucleotide is further purified by reverse phase HPLC employing gradient elution. Polynucleotides labelled with the atropisomer xanthene compounds of the present invention may be additionally labelled with moieties that affect the rate of elecxrophoretic migration, i.e. mobility-modifying labels. Mobility-modifying labels include polyethyleneoxy units, -(CH₂CH₂O)_n- where n may be 1 to 100 (Grossman, U.S. Patent No. 5,624,800). Preferably, n is from 2 to 20. The polyethyleneoxy units may be interspersed with phosphate groups. Specifically labelling atropisomer xanthene-labelled polynucleotides with additional labels of polyethyleneoxy of discrete and known size allows for separation by electrophoresis, substantially independent ofthe number of nucleotides in the polynucleotide. That is, polynucleotides of he same length may be discriminated upon by the presence of spectrally resolvable dye labels and mobility-modifying labels. Polynucleotides bearing both dye labels and mobility-modifying labels may be formed enzymatically by ligation or polymerase extension ofthe single-labelled polynucleotide or nucleotide constituents.

V.5 METHODS

Methods requiring simultaneous detection of multiple spatially-overlapping analytes may benefit from substantially pure atropisomers of asymmetric xanthene dyes as labels. The atropisomer xanthene compounds ofthe present invention are well suited for any method utilizing fluorescent detection, such as polymerase chain reaction (PCR) amplification, DNA sequencing, antisense transcriptional and translational control of gene expression, genetic analysis, and DNA probe-based diagnostic testing (Kricka, L. (1992) Nonisotopic DNA Probe Techniques, Academic Press, San Diego, pp.3-28). Fluorescence detection of fluorescent dye-labelled oligonucleotides is the basis for nucleic acid sequence detection assays such as 5' exonuclease assay (Livak, U.S. Patent No. 5,723,591), FRET hybridization (Tyagi, S. and Kramer, F. (1996) "Molecular Beacons: Probes that fluoresce upon hybridization", Nature Biotechnology, 14:303-08), genetic linkage mapping (Dib (1996) "A comprehensive genetic map ofthe human genome based on 5,264 microsatellites", Nature 380:152-54) and oligonucleotide-ligation assay (Grossman (1994) "High-density multiplex detection of nucleic acid sequences: oligonucleotide ligation assay and sequence-coded separation", Nucl. Acids Res. 22:4527- 34).

The present invention is particularly well suited for detecting classes of differently- labelled polynucleotides that have been subjected to a biochemical separation procedure, such as electrophoresis (Rickwood and Hames, Eds., Gel Electrophoresis of Nucleic Acids: A Practical Approach, IRL Press Limited, London, 1981). The electrophoretic matrix may be a sieving polymer, e.g. crosslinked or uncrosslinked polyacrylamide, or other amide- containing polymer, having a concentration (weight to volume) of between about 2-20 weight percent (Madabhushi, US Patent Nos. 5,552,028; 5,567,292; 5,916,426). The electrophoretic matrix may be configured in a slab gel or capillary format (Rosenblum, (1997) Nucleic Acids Res. 25:3925-29; Mathies, US Patent No. 5,274,240).

V.5A PRIMER EXTENSION

In a preferred category of methods referred to herein as "fragment analysis" or "genetic analysis" methods, polynucleotide fragments labelled with fluorescent dyes, including substantially pure atropisomeric xanthene compounds, are generated through template-directed enzymatic synthesis using labelled primers or nucleotides, e.g. by ligation or polymerase-directed primer extension. The polynucleotide fragments may be subjected to a size-dependent separation process, e.g., electrophoresis or chromatography, and the separated fragments are detected subsequent to the separation, e.g., by laser-induced fluorescence (Hunkapiller, U.S. Patent No. 4,811,218). Multiple classes of polynucleotides may be separated simultaneously and the different classes are distinguished by spectrally resolvable labels, including dyes ofthe invention. In electrophoresis, the classes separate on the basis of electrophoretic migration rate.

V.5B DNA SEQUENCING Preferably, the chain termination methods of DNA sequencing, i.e. dideoxy DNA sequencing, or Sanger-type sequencing, and fragment analysis is employed (Sanger (1977) "DNA sequencing with chain-terminating inhibitors", Proc. Natl. Acad. Sci. USA 74:5463- 5467). Exemplary chain-terminating nucleotide analogs include the 2',3'-dideoxynucleoside 5'-triphosphates (ddNTP) which lack the 3'-OH group necessary for 3' to 5' DNA chain elongation. Primers or ddNTP may be labelled with the substantially pure atropisomer xanthene dyes ofthe invention and detected by fluorescence after separation ofthe , fragments by high-resolution electrophoresis. Dyes can be linked to functionality on the 5' terminus ofthe primer, e.g. amino (Fung, U.S. Patent No.4,757,141), on the nucleobase of a primer; or on the nucleobase of a dideoxynucleotide, e.g. via alkynylamino linking groups (Khan, U.S. Patent No. 5,770,716; and 5,821,356; Hobbs, U.S. Patent No. 5,151,507).

Each ofthe terminators bears a different fluorescent dye and collectively the terminators ofthe experiment bear a set of dyes including one or more from the dyes ofthe invention. In a preferred fragment analysis method, fragments labelled with dyes are identified by relative size, i.e. sequence length. Correspondence between fragment size and sequence is established by incorporation ofthe four possible terminating nucleotides ("terminators") and the members of a set of spectrally resolvable dyes (Bergot, U.S. Patent No. 5,366,860). The set of spectrally resolvable dyes may include at least one substantially pure atropisomeric xanthene compound.

V.6 LIGATION

The covalent joining of nucleic acid probes by ligase enzymes is one ofthe most useful tools available to molecular biologists. When two probes are annealed to a template nucleic acid where the two probes are adjacent and without intervening gaps, a phosphodiester bond can be formed between a 5' terminus of one probe and the 3' terminus ofthe other probe by a ligase enzyme, (Whiteley, U.S. Patent No. 4,883,750; Landegren, (1988) "A ligase mediated gene detection technique", Science 241:1077-80; Nickerson, "Automated DNA diagnostics using an ELISA-based oligonucleotide assay" (1990) Proc. Natl. Acad. Sci USA 87:8923-27). Oligonucleotide ligation assays detect the presence of specific sequences in target DNA sample. Where one or both probes are labelled with a dye, the ligation product may be detected by fluorescence. One or both probes may be labelled with a substantially pure atropisomeric xanthene dye. Ligation products may be detected by electrophoresis, chromatography, or other size- or charge-based separation method. V.7 AMPLIFICATION

The atropisomer xanthene compounds ofthe invention find applications as labels on 5'-labelled oligonucleotide primers for the polymerase chain reaction (PCR) and other nucleic acid amplification and selection methods. PCR applications include the use of labelled oligonucleotides for genotyping by variable number tandem repeat (VNTR), short tandem repeat (STR), and microsatellite methods of amplification of repeat regions of double-stranded DNA that contain adjacent multiple copies of a particular sequence, with the number of repeating units being variable. Preferably, in such PCR genotyping methods, the PCR primer is labelled with an atropisomer xanthene ofthe invention. In a particularly preferred embodiment, atropisomer xanthene compounds may be used in quantitative methods and reagents that provide real time or end-point measurements of amplification products during PCR (US Patent Nos. 5,210,015; 5,538,848). The exonuclease assay (Taqman®) employing fluorescent dye-quencher probes (US Patent 5,723,591; Mullah, (1998) "Efficient synthesis of double dye-labelled oligodeoxyribonucleotide probes and their application in a real time PCR assay", Nucl. Acids Res. 26:1026-1031) gives direct detection of polymerase chain reaction (PCR) products in a closed-tube system, with no sample processing beyond that required to perform the PCR. In the Taqman assay, the polymerase that conducts primer extension and amplifies the polynucleotide also displaces and cleaves a probe annealed to target sequence by 5' to 3' exonuclease activity. In a Taqman-type assay, the probe is self- quenching, labelled with fluorescent dye and quencher moieties, either of which may be dyes ofthe invention. Spectral overlap allows for efficient energy transfer (FRET) when the probe is intact (Clegg, (1992) "Fluorescence resonance energy transfer and nucleic acids", Meth. Enzymol. 211 :353-388). When hybridized to a target sequence, the probe is cleaved during PCR to release a fluorescent signal that is proportional to the amount of target-probe hybrid present (US Patent Nos. 5,538,848; 5,723,591).

The progress of amplification can be monitored continuously, i.e. real-time detection. Spectrally-resolvable atropisomer xanthene dyes ofthe invention are useful in genotyping experiments after PCR amplification of target. In particular, a set of primer oligonucleotides, labelled at the 5' terminus, each with different dyes, can amplify multiple loci and discriminate single nucleotide polymorphisms (SNP) and alleles. Electrophoretic separation ofthe dye-labelled amplification products, with size standards, establishes a profile or characteristic data set indicating a certain genotype dependent on the set of primer sequences.

V.7A HYBRIDIZATION ASSAYS

Certain fluorescent dye-quencher probes which hybridize to target nucleic acids are useful in hybridization assays. When the probe is not hybridized to target, the probe may attain conformations that allow spatial proximity between the fluorescent dye and the quencher moieties resulting in fluorescence quenching. Upon hybridization to target, the moieties are physically separated, quenching ceases or diminishes, and fluorescence increases. Where the fluorescence is detectable or quantitated, the presence of target sequence in the sample is deduced. The atropisomeric dyes ofthe invention can also be employed as the fluorescent dye or the quencher moiety. Fluorescent dye-quencher probes with self-complementary sequences that form a "hairpin" region, so called "Molecular beacons" (Tyagi and Kramer) undergo the fluorescent change upon hybridization to their complementary target sequence, e.g. in situ quantitation of mRNA in living cells. Hybridization probes labelled with different fluorescent dyes, including the atropisomeric dyes ofthe invention, enable multiplex, homogeneous hybridization assays to be carried out in sealed reaction tubes.

V.8 CHROMATOGRAPHY

The aforementioned methods employing substrates labelled with substantially pure atropisomer xanthene compounds may also be conducted where the labelled substrates are detected by chromatography (HPLC ofMacromolecules, A Practical Approach, Second Edition, R.W.A. Oliver, Ed. (1997) Oxford University Press). The well established techniques of HPLC enable the separation of large substrates such as polynucleotides under reverse phase conditions where the sample substrate is dissolved and eluted in aqueous organic mobile phase from sorptive ion-exchange or hydrophobic interactions with an immobilized solid phase. When a chiral substrate such as a polynucleotide, polypeptide, or polysaccharide is labelled with a racemic mixture of atropisomeric xanthene compounds, diastereomers result. Essentially a redundant set of analytes are created which may obscure the analytical result. Analysis ofthe resulting diastereomeric mixture may lead to double peaks, broad peaks, and other limiting artifacts under the high- resolution conditions of HPLC. This problem is especially exacerbated where the chiral substrate is a mixture of closely related compounds, such as the nested set of polynucleotide fragments generated by the S anger sequencing method. Use of a substantially pure atropisomeric form of xanthene compounds as labels for chiral substrates prevents this unwanted hindrance to analysis by removing one ofthe diastereomers. The surprising and unexpected benefit ofthe invention may be exemplified by sharper peaks, less split peaks, and better resolution in general.

V.9 KITS

The invention includes kits comprising the substantially pure atropisomer xanthene compounds ofthe invention and/or their labelled conjugates. In one embodiment, the kits are useful for conjugating an atropisomer xanthene compound with a linking moiety to another molecule, i.e. a substrate. Such kits generally comprise an atropisomer xanthene ofthe invention including an optional linking moiety and reagents, enzymes, buffers, solvents, etc. suitable for conjugating the dye to another molecule or substance. The atropisomer xanthene may be an acceptor or donor of an energy-transfer dye.

In one embodiment, the kits are useful for labelling enzymatically synthesized oligonucleotides and polynucleotides with the atropisomer xanthenes of the invention. Such kits generally comprise a labelled enzymatically-incorporatable nucleotide or nucleotide analog according to the invention, a mixture of enzymatically-incorporatable nucleotides or nucleotide analogs capable of supporting continuous primer extension and a polymerase enzyme. Preferably, the labelled erizymatically-incorporatable nucleotide or nucleotide analog is a compound according to structure IV, most preferably a labelled terminator. Preferred polymerases are thermostable, such as AMPLITAQ^® DNA polymerase FS (Applied Biosystems, Foster City, CA).

Alternatively, the kit may include one or more primers. The primers may be labelled with atropisomer xanthenes and energy-transfer dyes including atropisomer xanthenes.

V.10 EXAMPLES

The invention will be further clarified by a consideration ofthe following examples, which are intended to be purely exemplary ofthe present invention and not to in any way limit its scope.

Example 1 Preparation of menthyl carbamate diastereomers of C-l aminomethyl, C-l 9 carboxy fluorescein, la and lb. The hydrochloride salt of C-l aminomethyl, C-l 9 carboxy fluorescein (5.16 gm, 11.6 mMol, 441.8 MW; Shipchandler (1987) Anal. Biochem. 162:89-101) was dissolved in 50 ml of deionized formamide and 10.2 ml diisopropylethylamine. (-) Menthyl chloroformate (3.06 gm, 3.0 ml, 14 mMol, 219 MW; Aldrich Chemical, Milwaukee, Wl; Jour. Chem. Soc, Chem. Commun. (1987) 470; Yodo (1988) Chem. Pharm. Bull. 36:902) was added dropwise with stirring at room temperature under argon. After 1.5 hours, TLC analysis (ethyl acetate/hexane : 4/1) showed partial conversion of reactant to a higher Rf spot. Another 1 ml (-) Menthyl chloroformate was added and stirring was continued for another 0.5 hour. TLC analysis showed complete conversion to the higher Rf product. Dilution ofthe reaction mixture with saturated aqueous NaHCO₃ was followed by extraction with 500 ml ethyl acetate. The aqueous fraction was acidified to pH 3 and extracted with ethyl acetate. The combined organic fractions were dried over Na₂SO₄, filtered and concentrated under vacuum to give 4.5 gm, 66% yield of a mixture of la and lb as a yellow powder (Figure la).

Example 2 Separation and isolation of diastereomers la and lb by HPLC.

Crude diastereomers la and lb were separated and purified by a two stage chromatography process on an open column, flash reverse phase column rough separation, followed by preparative reverse phase HPLC. • The mixture of la and lb was dissolved in ethyl acetate and adsorbed on C-l 8 reverse phase silica gel. The solvent was removed under vacuum and the solid was loaded on the top of a pre-equilibrated C-l 8 reverse phase column (21cm length x 6cm diameter). The diastereomers were separated and eluted with 25% CH₃CN in 100 mM TEAA (triethylammonium acetate) by collecting fractions. The fractions were analyzed by analytical reverse phase HPLC on a C-l 8 column (Metachem ODS3,

25cm length x 4.6mm inner diameter) with a linear gradient of 25% to 35% CH₃CN in 100 mM TEAA from 0 to 30 minutes at 1.0 ml/min flow rate and 260 nm UV detection. The fractions that contained the first eluting diastereomer of at least 75% purity were combined and concentrated under vacuum to an orange oil. The first eluting diastereomer was arbitrarily assigned structure la. The fractions that contained the second eluting diastereomer of at least 75% purity were combined and concentrated under vacuum to an orange oil. The second eluting diastereomer was arbitrarily assigned structure lb. Diastereomer la was purified to 99% isomeric purity by preparative reverse phase HPLC by loading 600-800 mg of la purified to 75% purity by the flash process, dissolved in 500 ml of 100 mM TEAA on to a Metachem ODS3 8μ column (Waters Prep LC 2000 System) and eluting under a gradient of 0 to 10% CH₃CN in 100 mM TEAA over 16 min., 10 to 35% CH3CN over 80 min., then hold at 35% CH₃CN for 32 min., at a flow rate of 40 ml/min., with UV detection at 260 nm. Fractions were collected and analyzed by the analytical reverse phase HPLC conditions above (Figure lb). Fractions with isomeric purity of at least 99% were combined, acidified to pH 2 with 6N HCI and extracted with ethyl acetate. The ethyl acetate fraction was washed with saturated NaCl, dried over anhydrous Na₂SO₄, concentrated under vacuum, precipitated with hexane, filtered, and dried to yield 300 to 500 mg of la as a bright yellow solid. 1H NMR (Acetone-d6) δ 9.85, 2H, br; 9.10, IH, br; 8.35, IH, d; 8.15, IH, d; 7.83, IH, s; 7.43, IH, br; 6.95, IH, s; 6.70, 4H, m; 4.60, 3H, m; 1.90, 2H, m; 1.65, 2H, m; 1.45, IH, m; 1.30, 2H, m; 0.89, 3H, d; 0.82, 3H, d; 0.78, 3H, d. Electrospray Mass Spectroscopy: 610 (M + Na), 588.5 (M + H),

Diastereomer lb is purified by the same preparative reverse phase HPLC process.

Example 3 Synthesis of atropisomer amine 2a

Diastereomer la (1.1 gm, 1.87 mmoles, 587.6 MW) was dissolved in 100 ml water and cooled to 0 °C. Concentrated sulfuric acid (15 ml) was added dropwise to give a brownish solution (Figure 2a). The temperature was allowed to rise to room temperature and the mixture was stirred overnight. The mixture was added slowly to

1.5 ml of ice water and then adsorbed on pre-equilibrated C-l 8 silica gel (4 cm length x 3 cm diameter). The support was washed with water until the pH ofthe eluent was neutral. The crude product was eluted with 200 ml CH₃OH which was concentrated under vacuum and dried to yield atropisomer 2a C-l aminomethyl, C-l 9 carboxy fluorescein sulfate salt as an orange solid (0.93 gm, 95% yield, 503.4 MW). 1H NMR (methanol-d4) δ 8.43, IH, d; 8.34, IH, d; 7.92, IH, s; 7.23, 3H, m; 7.06, IH, d; 6.98, IH, d; 4.58, 2H, s. The enantiomeric purity of hydrolyzed and purified 2a was analyzed by chiral column HPLC (Regies (S,S) Whelk-01 10-100 Kromasil FEC column, 25 cm length x

4.6 mm ID). The sample 2a was dissolved in water and eluted with a gradient of 0 to 35%o ethanol in water containing 0.1 % acetic acid over 30 minutes at 1 ml/min. with 254 nm UV detection (Figure 2c) and distinguished from the racemic mixture (Figure 2b).

Example 4 Synthesis of atropisomer amine 2b

Diastereomer lb is hydrolyzed, purified, and analyzed to give atropisomer 2b by the same processes as Example 3 (Figure 2a).

Example 5 Synthesis of atropisomeric trifluoroacetamide 3 a

Atropisomer 2a as the sulfate salt (0.93 gm, 1.84 mmoles, 503.4 MW) was dissolved in 15 ml ethanol. Triethylamine (1.8 ml, 13 mmoles) and ethyl trifluoroacetate (2.2 ml, 18 mmoles) were slowly added (Figure 2a). The mixture was stirred at room temperature under argon for 2.5 hours. Volatiles were removed under vacuum and the resulting residue was dissolved in 300 ml ethyl acetate and washed with 2 x 50 ml of 5% HCI. The ethyl acetate fraction was dried over anhydrous Na₂SO , filtered and concentrated under vacuum to yield atropisomeric trifluoroacetamide 3a as an orange solid (0.92 gm, 100% yield, 501.4 MW). 1H NMR (methanol-d4) δ 8.35, IH, d; 8.15, IH, d; 7.80, IH, s; 6.82, IH, s; 6.65, 4H, m; 4.82, 2H, s.

Example 6 Synthesis of atropisomeric trifluoroacetamide 3b

Atropisomer 2b is converted to atropisomeric trifluoroacetamide 3b by the same process and analyzed by the same methods as Example 5 (Figure 2a).

Example 7 Synthesis of atropisomeric NHS ester 4a

Atropisomeric trifluoroacetamide 3a (0.92 gm, 1.83 mmoles, 501.4), N- hydroxysuccinimide (0.85 gm, 7.3 mmoles), and l-[3-(dimethylamino)propyl]-3- ethylcarbodiimide hydrochloride (DAE) (1.05 gm, 5.5 mmoles) were dissolved in 24 ml ethyl acetate and 12 ml of l-methyl-2-pyrrolidinone (NMP) and stirred at room temperature under argon for 2.5 hours (Figure 3). The mixture was diluted with 300 ml ethyl acetate and washed with 2 x 80 ml of 5% HCI, dried over anhydrous Na₂SO₄, filtered, concentrated under vacuum, and adsorbed on silica gel. The silica gel with adsorbed product was loaded on the top of a dry-packed column of silica gel (12 cm length x 3 cm ID) and eluted with ethyl acetate:hexane / 2:1. Fractions containing atropisomeric NHS ester 4a were collected and combined, concentrated under vacuum and precipitated from hexane to yield 4a as a bright yellow solid (0.74 gm, 67% yield, 598.4 MW). 1H NMR (methanol-d4) δ 8.42, IH, d; 8.22, IH, d; 7.95, IH, s; 6.81, IH, s; 6.68, 4H, m; 4.82, 2H, s; 2.85, 4H, s.

Example 8 Synthesis of atropisomeric NHS ester 4b

Atropisomeric trifluoroacetamide 3b is converted to atropisomeric NHS ester 4b by the same process and analyzed by the same methods as Example 7 (Figure 3).

Example 9 Synthesis of 2- [(2-Fmoc-aminoethoxy)(hydroxyphosphoryl)oxy] acetic acid NHS 5

Protected phosphodiester linker synthon 5 was prepared by reacting methyl glycolate 6 (4.5 eq.) with cyclic phosphoramidite Amino-Link™ 7 (1 eq.) (Connell (1987) BioTechniques 5:342-348; US Patent 4,757,151) and 4-N,N-dimethylaminopyridine

(DMAP) (0.1 eq.). The mixture was stirred at ambient temperature for 1 hour. After the reaction was complete (TLC analysis), the solution was cooled with ice-bath and then treated with a solution of 3-chloroperoxybenzoic acid (4 eq.) in methylene chloride. The ice-bath was removed. After 30 minutes, an aqueous solution of NaHSO₃ (10%) was added. The mixture was diluted with ethyl acetate. The organic layer was washed with NaHSO₃ (10%), saturated solution of NaHCO₃, and dried with Na₂SO₄. The crude product was purified by flash chromatography to afford ester 8, which was heated at reflux for 3 hours (36 mM, 1 eq.) in methylethylketone and Nal (10 eq.). The crude demethylated phosphodiester was dissolved in 0.3 M solution of LiOH (5 eq.) in H₂O/CH₃OH : 1/3) and stirred overnight to cleave the methyl ester. Solvent was removed to afford crude compound 9 which was then dissolved in aqueous Na₂CO₃ (5%). N-(9- Fluorenylmethoxy-carbonyloxy)succinimide (FmocOSu, 1.5 eq.) in THF was added in one portion and stirred at ambient temperature for 3 hours. The crude product was diluted with ethyl acetate and washed with 10% aqueous HCI. The organic layer was dried with Na₂SO₄, filtered, concentrated under vacuum, and purified by flash cliromatography to afford Fmoc-acid 10 as a yellow oil.

Fmoc-acid 10 was dissolved in anhydrous CH₂C1₂ (1 eq.). N-hydroxysuccinimide (4 eq.) was added. The solution was cooled with an ice-bath and then treated with dicyclohexyl carbodiimide (DCC, 2 eq.). The ice-bath was then removed, and stirring was continued for 2 hours (with TLC analysis). When the reaction was complete, ethyl acetate was added and the solution was washed with 5% aqueous HCI. Removal of solvent gave 2-[(2-Fmoc-aminoethoxy)(hydroxyphosphoryl)oxy]acetic acid NHS 5 (Figure 4). Example 10 Synthesis of 7-propargylphosphorylamino-7-deaza-ddATP 11

7-Deaza-7-propargylamino-ddATP 12 (7-(3-amino- 1 -propynyl)-2 ' ,3 '-dideoxy-7- deazaadenosine-5 '-triphosphate; US Patent Nos. 5,047,519 and 5,151,507) was suspended in 250 mM bicarbonate (pH 9.0) and a solution of 2-[(2-Fmoc- aminoethoxy)(hydroxyphosphoryl)oxy] acetic acid NHS 5 in DMSO was added. After 1 hour, the reaction mixture was purified by HPLC (AX-300 anion exchange). The product fractions were collected, concentrated to dryness, and purified by RP HPLC (C-l 8 reverse phase) to afford Fmoc-linker ddATP 13. Concentrated ammonium hydroxide (28-30%) was added to Fmoc-linker ddATP 13 and the solution was heated to 55°C for 20 minutes. Concentration under vacuum gave crude 7-propargylphosphorylamino-7-deaza-ddATP 11 which was purified by C-l 8 reverse phase HPLC (Figure 5).

Example 11 Synthesis of Fmoc-aminomethyl-NHS-FAM 14

Fmoc-aminomethyl-NHS-FAM 14 was prepared by reacting the fluorenylmethoxy-carbonyloxy ester of N-hydroxysuccinimide (Fmoc-OSu) with the HCI salt ofp-aminomethylbenzoic acid (both commercially available) in the presence of base to form the expected N-Fmoc derivative. This product was then reacted with N- hydroxysuccinimide in the presence of DCC to form the NHS ester ofthe benzoic acid carboxyl group. This NHS-ester, N-Fmoc derivative of/?-aminomethylbenzoic acid having the structure:

was then reacted with atropisomer C-l aminomethyl, C-l 9 carboxy fluorescein 2a, purified by the method of Example 2, followed by reaction with N-hydroxysuccinimide in the presence of DCC to produce substantially pure atropisomer, Fmoc-aminomethyl-NHS- FAM 14 (Figure 6).

Example 12 Synthesis of Aminomethylbenzamide-aminomethyl-FAM- propargylphosphorylamino-ddATP 15 A solution of Fmoc-aminomethyl-NHS-FAM 14 in DMSO was added to 7- propargylphosphorylamino-7-deaza-ddATP 11 suspended in 250 mM bicarbonate (pH 9.0). The reaction mixture was placed in the dark at ambient temperature for 2 hours. The Fmoc-amino protected product 16 was purified by HPLC (AX-300 anion exchange), then heated at 55°C in concentrated ammonium hydroxide (28-30%) for 20 minutes to hydrolyze the Fmoc group. Concentration under vacuum gave crude, substantially pure atropisomer, aminomethylbenzamide-aminomethyl-FAM-propargylphosphorylamino- ddATP 15 which was purified by C-l 8 reverse phase HPLC (Figure 6).

Example 13 Synthesis of NHS-rhodamine dye 17 Bicyclic amine 18 (12.8 gm, 47 mmole, US Patent No. 5,688,808), l-bromo-3- chloropropane (29.3 gm, 187 mmole), sodium iodide (56.4 gm, 376 mmole) and sodium bicarbonate (7.9 gm, 94 mmole) was refluxed in 150 ml CH₃CN for 18 hours. The mixture was cooled to room temperature, filtered, and evaporated. The filter cake was washed with 300 ml hexane which was combined with the filtrate and washed with 2 x 50 ml water and 50 ml saturated NaCl, dried over MgSO₄, filtered, and concentrated under vacuum. The product was purified by chromatography on silica gel, eluting with hexane/ethyl acetate : 20/1, to give tricyclic amine pivalate ester 19 as a pale yellow oil (9.5 gm, 30 mmole, 64% yield). The ester of 19 was hydrolyzed in a solution of lithium hydroxide monohydrate (2.6 gm, 60 mmole) in 15 ml water and 120 ml methanol. After stirring for one hour at room temperature, the mixture was concentrated under vacuum and dissolved in 30 ml 1M HCI which was extracted with 3 x 100 ml of diethylether. The combined ether extracts were washed with 50 ml of 200 mM pH 7 phosphate buffer, dried over MgSO₄, filtered and concentrated under vacuum to give tricyclic amine 20 as a brown solid (Figure 7). Tricyclic amine 20 and 3,6-dichloro, 4-isopropylcarboxylate phthalic anhydride 21 were refluxed in toluene to give Friedel-Craft acylation product ketone 22 (Abs. max 400 nm) (Figure 8).

Cyclization of 22 with 20 in phosphoryl trichloride and chloroform at reflux gave 23 as a mixture of isopropylcarboxylate regioisomers. After cleavage ofthe isopropyl group, the rhodamine carboxylic acid 24 was converted to NHS-rhodamine dye 17 (Figure 8).

Example 14 Synthesis of phosphate-linker, energy-transfer terminator ddATP 25 Aminomethylbenzamide-aminomethyl-FAM-propargylphosphorylamino-ddATP 15 from Example 12 was suspended in a solution of 250 mM bicarbonate (pH 9.0). A solution of NHS ester 17 (US Patent 5,847,162 for synthesis) in DMSO was added. The reaction mixture was placed in the dark at ambient temperature for 2 hours. Purification was done by HPLC, AX-300 anion exchange and then C-l 8 reverse phase to afford pure energy-transfer ddATP terminator 25 (Figure 9).

Example 15 Synthesis of bis-trifluoroacetamide rhodamine NHS 27

Rhodamine dye 28 was converted to the bis-trifluoroacetamide 29 by treatment with trifluoroacetic anhydride and triethylamine in diethylether at room temperature. The carboxylic acid was converted to the NHS ester with dicyclohexylcarbodiimide and N- hydroxysuccinimide to give bis-trifluoroacetamide rhodamine NHS 27 (Figure 10).

Example 16 Synthesis of Aminomethylbenzamide-aminomethyl-FAM- propargylethoxyamino-ddTTP 32

5-(3-Aminoethoxy-l -propynyl)-2',3 '-dideoxythymidine-5 '-triphosphate 30 (US Patent 5,821,356) was reacted with substantially pure atropisomer, Fmoc-aminomethyl- NHS-FAM 14 under the same conditions as Example 12 to give Fmoc- aminomethylbenzamide-aminomethyl-FAM-propargylethoxyamino-ddTTP 31 which was purified by anion-exchange HPLC. The Fmoc group of 31 was cleaved to give substantially pure atropisomer, aminomethylbenzamide-aminomethyl-FAM- propargylethoxyamino-ddTTP 32 (Figure 11).

Example 17 Synthesis of energy-transfer terminator ddTTP 26

Following the conditions of Example 14, substantially pure atropisomer, aminomethylbenzamide-aminomethyl-FAM-propargylethoxyamino-ddTTP 32 was suspended in a solution of 250 mM bicarbonate (pH 9.0). A solution of bis- trifluoroacetamide rhodamine NHS ester 27 in DMSO was added. The reaction mixture was placed in the dark at ambient temperature for 2 hours. Ammonium hydroxide was added to cleave the trifluoroacetamide groups. Purification was done by HPLC, AX-300 anion exchange and then C-l 8 reverse phase to afford pure energy-transfer ddTTP terminator 26 (Figure 12).

Example 18 Sequencing of pGEM with phosphate-linker, energy-transfer terminator ddATP 25 Following the conditions of U.S. Patent Nos. 5,770,716; 5,948,648; and 6096875, the energy-transfer ddATP terminator 25 was used with other standard reagents in a S anger-type, one-color automated DNA sequencing experiment. The terminator nucleotide 25 was tested as the racemic mixture of atropisomers (top electropherogram) and as a substantially pure atropisomer (bottom electropherogram) shown in Figures 13a and 13b.

The dye-terminator sequencing reactions were performed using AmpliTaq DNA Polymerase, FS following protocols provided in the ABI PRISM™ Dye Terminator Cycle Sequencing Core Kit Manual (Applied Biosystems p/n 402116). Sequencing ofthe pGEM-3Zf(+) template was conducted with unlabelled -21 M13 sequencing primer (forward). Reagents, including buffer, unlabelled primer, AmpliTaq DNA Polymerase, FS, were from an ABI PRISM™ Dye Terminator Core Kit (Applied Biosystems p/n 402117). The dNTP mix consisted of 2 mM each of dATP, dCTP, dITP, and dUTP or dTTP. A premix of reaction components was prepared including: 5X Buffer 4.0μL; dNTP mix 1.OμL; Template:pGEM^®-3Zf(+), 0.2μg/μL, 2.0 μL; Primer: -21 M13 (forward), 0.8 pmol/μL, 4.0 μL; AmpliTaq DNA Polymerase, FS, 0.5 μL; and H₂O 3.5 μL, wherein all quantities are given on a per reaction basis.

Reactions were assembled in 0.5 ml tubes adapted for the Perkin-Elmer 480 DNA Thermal Cycler (Applied Biosystems p/n N801 - 100 or 0.2 ml tubes for the Applied Biosystems Gene Amp PCR System 9700). From 1 to 250 pmol ofthe dye terminator was added to each reaction. 30 μL of mineral oil was added to the top of each reaction to prevent evaporation (when using the Applied Biosystems 480 Thermal Cycler). Reaction volumes were 20 μL, including 15 μL ofthe above reaction premix, a variable amount of dye labelled terminator, and a sufficient volume of water to bring the total reaction volume up to 20 μL. Reactions were thermocycled as follows: 96°C for 30 sec, 50°C for 15 sec, and 60°C for 4 min, for 25 cycles; followed by a 4°C hold cycle.

All reactions were purified by spin-column purification on Centri-Sep spin columns according to manufacturer's instructions (Princeton Separations p/n CS-901). Gel material in the column was hydrated with 0.8 mL deionized water for at least 30 minutes at room temperature. After the column was hydrated and it was determined that no bubbles were trapped in the gel material, the upper and lower end caps ofthe column were removed, and the column was allowed to drain by gravity. The column was then inserted into the wash tubes provided in the kit and centrifuged in a variable speed microcentrifuge at 1300g for 2 minutes, removed from the wash tube, and inserted into a sample collection tube. The reaction mixture was carefully removed from under the oil and loaded onto the gel material and the tube re-centrifuged. Eluted samples were then dried in a vacuum centrifuge.

Prior to loading onto a sequencing gel, the dried samples were resuspended in 25 μL of Template Suppression Reagent (Applied Biosystems p/n 401674), vortexed, heated to 95°C for 2 minutes, cooled on ice, vortexed again, and centrifuged (13,000 x g). 10 μL ofthe resuspended sample was aliquoted into sample vials (Applied Biosystems p/n 401957) adapted for the ABI PRISM™ 310 Genetic Analyzer (Applied Biosystems p/n 310-00-100/120). Electrophoresis on the 310 Genetic Analyzer was performed with sieving polymers and capillaries specially adapted for DNA sequencing analysis (PE Applied Biosystems p/n 402837 or 4313087 (polymer) and p/n 402840 (capillary)). In each case, the sieving polymer included nucleic acid denaturants. Samples were electrokinetically injected onto the capillary for 30 sec at 2.5 kV, and run for up to 2 hr at 10 to 12.2 kV with the outside wall ofthe capillary maintained at 50°C to generate electropherograms as sequencing data (Figures 13a-d).

The electropherograms in Figures 13a and 13b show the specific incorporation of the energy-transfer terminator 25 onto the 3' terminus of primer extension, polynucleotide fragments during single color sequencing reactions. The electropherograms plot the fluorescence intensity emitted (Emission maxima about 650 nm) by the acceptor rhodamine dye ofthe labelled fragments between about 20 to about 600 nucleotides in length as a function of time during an electrophoresis run on the ABI PRISM™ 310 Genetic Analyzer.

Eluting fragments from 119 to 242 base pairs are plotted in Figure 13a. Each of the fragments was 3' terminated by energy-transfer terminator 25. Figure 13b shows a more magnified view of fragments 148 to 205 base pairs. The three regions under the arrows in Figures 13a and 13b illustrate the surprising and unexpected improvement in separating fragments labelled with the substantially pure atropisomer form of 25 (bottom panels) relative to the separation of fragments labelled with the racemic mixture of 25. Better resolution ofthe fragments was observed in the bottom electropherogram with the substantially pure afropisomer than with the racemic mixture of atropisomers in 25. The locations marked with arrows are particular loci where substantially pure atropisomer form of 25 provided the unexpected benefit of better resolution. By contrast, use ofthe racemic mixture of atropisomeric form of 25 in labelling the chiral primer extension products led to diastereomeric populations of fragments which migrate electrophoretically at different rates, as exemplified by the broad, overlapping, and split peaks under the arrows in the top electropherogram.

Additionally, the bottom electropherogram shows more even peak heights throughout the sequencing ladder than was observed in the top electropherogram with the racemic mixture of atropisomers in 25.

Example 19 Sequencing of pGEM with sulfonate-linker, energy-transfer terminator ddATP 33

Following the general synthesis routes and conditions ofthe previous Examples, substantially pure atropisomer, sulfonate-linker, energy-transfer terminator ddATP 33 (Figure 14) was synthesized. Following the protocol and conditions of Example 18, 33 was used in single-color sequencing the pGEM target. Separately, the racemic mixture of 33 was also used in the same single-color sequencing experiment. Eluting fragments from 148 to 242 base pairs are plotted in Figure 13c. Each ofthe fragments was 3' terminated by energy-transfer terminator 33. The three regions under the arrows in Figure 13c illustrate the surprising and unexpected improvement in separating fragments labelled with the substantially pure atropisomer form of 33 (bottom panel) relative to the separation of fragments labelled with the racemic mixture of 33 (top panel). Better resolution ofthe fragments was observed in the bottom electropherogram with the substantially pure afropisomer than with the racemic mixture of atropisomers in 33.

Example 20 Sequencing of pGEM with energy-transfer terminator ddGTP 34 Following the general synthesis routes and reaction conditions ofthe previous

Examples, energy-transfer terminator ddGTP 34 (Figure 15) was synthesized. The atropisomer forms were separated at the final stage of synthesis, i.e. compound 34, by reverse-phase HPLC. Following the protocol and conditions of Example 18, a substantially pure atropisomer of 34 was used in single-color sequencing the pGEM target. Separately, the racemic mixture of 34 was also used in the same single-color sequencing experiment. Eluting fragments from 24 to 99 base pairs, detected at about 535 nm, are plotted in Figure 13d. Each ofthe fragments was 3' terminated by energy-transfer terminator 34. The regions under the arrows in Figure 13d illustrates the surprising and unexpected improvement in separating fragments labelled with the substantially pure atropisomer form of 34 (bottom panel) relative to the separation of fragments labelled with the racemic mixture of 34 (top panel). Better resolution of virtually every fragment was observed in the bottom electropherogram with the substantially pure atropisomer than with the racemic mixture of atropisomers in 34. The locations marked with arrows are particular loci where substantially pure atropisomer form of 34 provided the unexpected benefit of better resolution. Every fragment in the bottom panel, labelled with racemic 34, separates into two peaks, detracting from the utility ofthe data. Although only a few embodiments have been described in detail above, those having ordinary skill in the chemical and molecular biology arts will clearly understand that many modifications are possible in the illustrated embodiments without departing from the teachings thereof. All such modifications are intended to be encompassed within the following claims.

Claims (110)

1. We claim: 1. A substantially pure afropisomer compound having the structure:

   Z¹ is OH, NH , NHR, or NR₂, wherein each R is independently hydrogen, Cι-Cι₂ alkyl, phenyl, benzyl, aryl, heterocycle, or a linking moiety;

   Z² is 0, NH , ⁺NHR, or ⁺TSJR₂, wherein each R is independently hydrogen, C₁-C₁2 alkyl, phenyl, benzyl, aryl, heterocycle, or a linking moiety; X is carboxylate or sulfonate; and said structure includes aryl-substituted forms thereof.
2. 2. The substantially pure atropisomer compound ofclaim 1 wherein Z¹ is OH, Z² is O, and X is carboxylate.
3. 3. The substantially pure afropisomer compound ofclaim 1 wherein Z¹ is NR₂, Z² is ⁺NR , and X is carboxylate.
4. 4. The substantially pure atropisomer compound ofclaim 1 having the structure:

   wherein at least one of R¹, R⁴, R⁵, R¹¹, R¹³, R¹⁴, R¹⁷, R¹⁸, R¹⁹, R²⁰, Z¹, or Z² is a linking moiety selected from the group consisting of azido, monosubstituted primary amine, disubstituted secondary amine, thiol, hydroxyl, halide, epoxide, N- hydroxysuccinimidyl ester, carboxyl, isothiocyanate, sulfonyl chloride, sulfonate ester, silyl halide, chlorotriazmyl, succinimidyl ester, pentafluorophenyl ester, maleimide, haloacetyl, epoxide, alkylhalide, allyl halide, aldehyde, ketone, acylazide, anhydride, iodoacetamide and an activated ester.
5. 5. The compound of claim 4 wherein one of R¹ and R¹¹ is a linking moiety.
6. 6. The compound of claim 4 comprising one or more substituents R¹, R⁴, R⁵, R¹¹, R¹³, R¹⁴, R¹⁷, R¹⁸, R¹⁹, and R²⁰, each of which is independently fluorine, chlorine, Cι-C₈ alkyl, carboxylate, sulfate, sulfonate, alkylsulfonate, aminomethyl (-CH₂NH₂), aminoalkyl, 4-dialkylaminopyridinium, hydroxymethyl (-CH OH), methoxy (-OCH₃), hydroxyalkyl (-ROH), thiomethyl (-CH₂SH), thioalkyl (-RSH), alkylsulfone (-SO₂R), arylthio (-SAr), arylsulfone (-SO₂Ar), sulfonamide (-SO₂NR₂), alkylsulfoxide (-SOR), arylsulfoxide (-SOAr), amino (-NH₂), ammonium (-NH₃ ⁺), amido (-CONR ), nitrile (-CN), Cι-C₈ alkoxy (-OR), phenoxy, phenolic, tolyl, phenyl, aryl, benzyl, heterocycle, phosphonate, phosphate, quaternary amine, sulfate, polyethyleneoxy, and linking moiety.
7. 7. The compound of claim 4 wherein R¹³ and R¹⁴ taken together form benzo.
8. 8. The compound ofclaim 4 wherein at least one of R¹ , R⁴, R⁵, R¹¹, R¹³, and R¹⁴ is an electron-deficient heterocycle selected from 2-pyridyl, 3-pyridyl, 4-pyridyl, 2-quinolyl, 3-quinolyl, 4-quinolyl, 2-imidazole, 4-imidazole, 3-pyrazole, 4-pyrazole, pyridazine, pyrimidine, pyrazine, cinnoline, pthalazine, quinazoline, quinoxaline, 3- (l,2,4-N)-triazolyl, 5-(l,2,4-N)-triazolyl, 5-tetrazolyl, 4-(l-0, 3-N)-oxazole, 5-(l-0, 3- N)-oxazole, 4-(l-S, 3-N)-thiazole, 5-(l-S, 3-N)-thiazole, 2-benzoxazole, 2-benzothiazole, 4-(l ,2,3-N)-benzotriazole, and benzimidazole.
9. 9. The compound ofclaim 4 wherein at least one of R¹, R⁴, R⁵, R¹¹, R¹³, and R¹⁴ is phenyl or substituted phenyl.
10. 10. The compound ofclaim 4 wherein at least one of R¹, R⁴, R⁵, Rⁿ, R¹³, and R¹⁴ is naphthyl or substituted naphthyl.
11. 11. The compound of claim 4 wherein at least one of R¹ , R⁴, R⁵, R¹¹ , R¹³, and R¹⁴ is fluorine or chlorine.
12. 12. The compound of claim 4 wherein at least one of R¹ , R⁴, R⁵, R¹¹ , R¹³, and R¹⁴ is 2-pyridyl or 3-pyridyl.
13. 13. The compound ofclaim 4 wherein at least one of R¹, R⁴, R⁵, R¹¹, R¹³, and R¹⁴ is 2-quinolyl or 3-quinolyl.
14. 14. The compound ofclaim 4 wherein at least one of R¹, R⁴, R⁵, Rⁿ, R¹³, and R¹⁴ is methoxy (-OCH₃).
15. 15. The compound ofclaim 4 wherein at least one of R¹, R⁴, R⁵, R¹¹, R¹³, and R¹⁴ is aminomethyl (-CH₂NH₂).
16. 16. The compound ofclaim 4 wherein at least one of R¹⁷, R¹⁸, R¹⁹, and R²⁰ is chlorine.
17. 17. The compound ofclaim 4 wherein at least one of R¹⁷, R¹⁸, R¹⁹, and R²⁰ is fluorine.
18. 18. The compound ofclaim 4 wherein at least one of R¹⁷, R¹⁸, R¹⁹, and R²⁰ is

    4-dialkylaminopyridinium.
19. 19. The compound ofclaim 4 wherein at least one of R¹⁷, R¹⁸, R¹⁹, and R²⁰ is thiophenyl.
20. 20. The compound of claim 4 wherein at least one of R¹⁷, R¹⁸, R¹⁹, and R²⁰ is thio-4-carboxyphenyl.
21. 21. The compound of claim 4 wherein one of R¹⁸ and R¹⁹ is carboxyl and the other is hydrogen.
22. 22. The compound of claim 4 wherein one of R¹⁸ and R¹⁹ is a linking moiety and the other is hydrogen.
23. 23. The compound of claim 4 wherein R¹⁷ and R²⁰ are chlorine; one R¹⁸ and

    R¹⁹ is a linking moiety and the other is hydrogen; and X is carboxyl.
24. 24. The compound of claim 4 comprising a first bridging group which when taken together with a Z¹ nitrogen, the Z¹ -bonded carbon, and the R¹ -bonded carbon, forms a first ring structure having from 4 to 7 members; and

    • ■ optionally, a second bridging group which when taken together with a Z

    9 1 1 nitrogen, the Z -bonded carbon, and the R -bonded carbon forms a second ring structure having from 4 to 7 members.
25. 25. The compound of claim 24 wherein one or both of the first and second ring structures has five members.
26. 26. The compound of claim 25 wherein the five membered ring structure includes one gem disubstituted carbon.
27. 27. The compound of claim 29 wherein the gem substituents are Cι-C₈ alkyl.
28. 28. The compound of claim 27 wherein the gem substituents are methyl.
29. 29. The compound of claim 25 wherein the five membered ring is substituted with a linking moiety.
30. 30. A substantially pure atropisomer compound selected from the group consisting ofthe structures:
31. 31. An energy-transfer dye comprising: a donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto; an acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response; and a linker for linking the donor dye and the acceptor dye; wherein at least one ofthe donor dye and acceptor dye is a substantially pure atropisomer of a xanthene compound having the structure:

    wherein Z¹ and Z² are O, OH, NH₂, NHR, or NR₂; X is carboxylate or sulfonate, and aryl-substituted forms thereof.
32. 32. The energy-transfer dye ofclaim 31 wherein the donor dye is a substantially pure atropisomer of a xanthene compound and the acceptor dye is a cyanine, a phthalocyanine, a squaraine, a bodipy, a benzophenoxazine, a fluorescein, a dibenzorhodamine, or a rhodamine dye.
33. 33. The energy-transfer dye ofclaim 31 wherein the acceptor dye is a substantially pure afropisomer of a xanthene compound and the donor dye is linked to the xanthene compound and to a polynucleotide.
34. 34. The energy-transfer dye of claim 33 wherein the donor dye is linked to the 5'-terminus ofthe polynucleotide.
35. 35. The energy-transfer dye ofclaim 33 wherein the donor dye is linked to the 3'-terminus ofthe polynucleotide.
36. 36. The energy-transfer dye of claim 33 wherein the donor dye is linked to a nucleobase ofthe polynucleotide, wherein if the nucleobase is a purine, the linker is attached at the 8-position, if the nucleobase is a 7-deazapurine, the linker is attached at the 7-position or 8-position, and if the nucleobase is a pyrimidine, the linker is attached at the 5-position.
37. 37. The energy-transfer dye of claim 31 wherein the linker has the structures:

    O O

    — R²¹— Z- — or — R²¹— Z-C— R²²— R²³ — - wherein

    Z is selected from the group consisting of NH, S and O; R²¹ is a Cι-C₁₂ alkyl attached to the donor dye;

    R²² is a substituent selected from the group consisting of a C₁-C₁₂ alkyldiyl, a five and six membered ring having at least one unsaturated bond and a fused ring structure which is attached to the carbonyl carbon; and

    R²³ includes a functional group which attaches the linker to the acceptor dye.
38. 38. The compound ofclaim 37 wherein the linker has the structure:

    O

    II (CH₂)n— NH-C — and n ranges from 2 to 10.
39. 39. The energy-transfer dye of claim 37 wherein R²³ has the structure

    O

    _R _Z-C— wherein R 24 . is a Cι-C₁₂ alkyl.

    99
40. 40. The energy-transfer dye of claim 37 wherein R is a five or six membered ring selected from the group consisting of cyclopentene, cyclohexene, cyclopentadiene, cyclohexadiene, furan, thiofuran, pyrrole, isopyrole, isoazole, pyrazole, isoimidazole, pyran, pyrone, benzene, pyridine, pyridazine, pyrimidine, pyrazine oxazine, indene, benzofuran, thionaphthene, indole and naphthalene.
41. 41. The energy-transfer dye of claim 31 wherein the linker has the structure

    DONOR
42. 42. The energy-transfer dye of claim 31 wherein the linker has the structure
43. 43. The energy-transfer dye of claim 31 wherein the linker has the structure
44. 44. The energy-transfer dye ofclaim 31 in which the linker has the structure:

    wherein D is a donor dye, A is an acceptor dye and n is 1 or 2.
45. 45. The energy-transfer dye ofclaim 31 wherein the linker is attached at R¹, R .11 , R or R .19 of the substantially pure atropisomeric xanthene compound having the structure:
46. 46. The energy-transfer dye ofclaim 45 wherein a linker to the donor or the acceptor is attached to R¹ or R¹¹, and a linker to a polynucleotide is attached to R¹⁸ or

    R 19
47. 47. The energy-transfer dye ofclaim 45 wherein the linker is attached at one of positions Z¹ or Z² ofthe substantially pure atropisomeric xanthene compound.
48. 48. A labelled nucleoside or nucleotide having the formula:

    wherein DYE is a substantially pure atropisomer of a xanthene compound having the structure:

    Z is OH, NH₂, NHR, or NR₂, wherein each R is independently hydrogen, C₁-C₁₂ alkyl, phenyl, benzyl, aryl, heterocycle, or a linking moiety;

    Z² is O, ⁺NH₂, ⁺NHR, or ⁺NR₂, wherein each R is independently hydrogen, C1-C₁₂ alkyl, phenyl, benzyl, aryl, heterocycle, or a linking moiety; X is carboxylate or sulfonate; or

    DYE is an energy-transfer dye comprising a donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto; an acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response; and a linker for linking the donor dye and the acceptor dye; wherein at least one ofthe donor dye and acceptor dye is a said substantially pure afropisomer of a xanthene compound;

    B is a nucleobase;

    L is a linker; R²⁵ is H, monophosphate, diphosphate, triphosphate, thiophosphate, or phosphate analog; and

    R²⁶ and R²⁷, when taken alone, are each independently H, HO, F, or other moiety which blocks polymerase-mediated target-directed polymerization, or when taken together, R²⁶ and R²⁷ form 2'-3'-didehydroribose; and wherein DYE is optionally aryl-substituted.
49. 49. The labelled nucleoside or nucleotide of claim 48 wherein B is selected from the group consisting of uracil, thymine, cytosine, adenine, 7-deazaadenine, guanine, and 7-deazaguanosine.
50. 50. The labelled nucleoside or nucleotide ofclaim 48 in which L is:

    wherein n is 0, 1, or 2.
51. 51. The labelled nucleoside or nucleotide of claim 48 which is enzymatically incorporatable.
52. 52. The labelled nucleoside or nucleotide of claim 48 which is a terminator.
53. 53. The labelled nucleoside or nucleotide of claim 52 which has the structure:

    wherein R²⁶ and R²⁷, when taken alone, are each independently H, F, or other moiety which blocks polymerase-mediated target-directed polymerization, or when taken together form 2'-3'-didehydroribose.
54. 54. The labelled nucleoside or nucleotide of claim 48 which is enzymatically extendable.
55. 55. A labelled polynucleotide comprising a polynucleotide covalently attached to a label, wherein the label is a substantially pure afropisomer of a xanthene compound having the structure:

    Z¹ is OH, NH₂, NHR, or NR₂, wherein each R is independently hydrogen,

    Cι-C₁₂ alkyl, phenyl, benzyl, aryl, heterocycle, or a linking moiety;

    Z² is O, ⁺NH , ^"ΗHR, or ⁺NR₂, wherein each R is independently hydrogen, C₁-C₁₂ alkyl, phenyl, benzyl, aryl, heterocycle, or a linking moiety; X is carboxylate or sulfonate; or an energy-transfer dye comprising a donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto; an acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response; and a linker for linking the donor dye and the acceptor dye; wherein at least one ofthe donor dye and acceptor dye is a said substantially pure atropisomer of a xanthene compound; and wherein said substantially pure afropisomer of a xanthene compound is optionally aryl-substituted.
56. 56. The labelled polynucleotide ofclaim 55 comprising the formula:

    wherein DYE is a substantially pure atropisomer of a xanthene compound or an energy-transfer dye; B is a nucleobase; L is a linker;

    97 R is H, OH, halide, azide, amine, alkylamine, Cι-C₆ alkyl, allyl, Cι-C₆ alkoxy, OCH₃, or OCH₂CH=CH₂; and

    R²⁸ and R²⁹when taken alone, are each independently H, phosphate, internucleotide phosphodiester, or internucleotide analog; wherein the polynucleotide comprises 2 to 100 nucleotides.
57. 57. The labelled polynucleotide ofclaim 56 wherein B is selected from the group consisting of uracil, thymine, cytosine, adenine, 7-deazaadenine, guanine, and 7- deazaguanosine.
58. 58. The labelled polynucleotide ofclaim 55 comprising the formula:

    wherein DYE is a substantially pure atropisomer of a xanthene compound or an energy-transfer dye;

    B is a nucleobase; X is O, NH, or S; L is a linker; R²⁷ is H, OH, halide, azide, amine, alkylamine, Cι-C₆ alkyl, allyl, Cι-C₆ alkoxy, OCH₃, or OCH₂CH=CH₂; and

    9R

    R is internucleotide phosphodiester or internucleotide analog; wherein the polynucleotide comprises 2 to 100 nucleotides.
59. 59. The labelled polynucleotide ofclaim 58 wherein B is selected from the group consisting of uracil, thymine, cytosine, adenine, 7-deazaadenine, guanine, and 7- deazaguanosine.
60. 60. The labelled polynucleotide ofclaim 58 in which L is Cι-Cι₂ alkyldiyl.
61. 61. The labelled polynucleotide of claim 58 in which L comprises -(CH₂CH₂O)_n- where n is 1 to 100.
62. 62. A labelled polypeptide comprising a polypeptide covalently attached to a substantially pure afropisomer of a xanthene compound having the structure:

    Z¹ is OH, NH₂, NHR, or NR₂, wherein each R is independently hydrogen, C₁-C₁₂ alkyl, phenyl, benzyl, aryl, heterocycle, or a linking moiety;

    Z² is O, ⁺NEΪ₂, ⁺NHR, or ⁺NR₂, wherein each R is independently hydrogen, C₁-C₁₂ alkyl, phenyl, benzyl, aryl, heterocycle, or a linking moiety;

    X is carboxylate or sulfonate; or the polypeptide covalently attached to an energy-transfer dye comprising a donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto; an acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response; and a linker for linking the donor dye and the acceptor dye; wherein at least one ofthe donor dye and acceptor dye is the said substantially pure afropisomer of a xanthene compound; and wherein a said substantially pure atropisomer of a xanthene compound is optionally aryl-substituted.
63. 63. A phosphoramidite compound having the formula:

    wherein DYE is a substantially pure afropisomer of a xanthene compound having the structure:

    Z¹ is OH, NH₂, NHR, or NR₂, wherein each R is independently hydrogen, C₁-C₁₂ alkyl, phenyl, benzyl, aryl, heterocycle, or a linking moiety;

    Z² is O, ^'+NH₂, ⁺NHR, or ⁺NR , wherein each R is independently hydrogen, C₁-C₁₂ alkyl, phenyl, benzyl, aryl, heterocycle, or a linking moiety;

    X is carboxylate or sulfonate; or

    DYE is an energy-transfer dye comprising a donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto; an acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response; and a linker for linking the donor dye and the acceptor dye; wherein at least one ofthe donor dye and acceptor dye is a said substantially pure atropisomer of a xanthene compound; and wherein DYE is optionally aryl-substituted;

    L is a linker; R³⁰ and R³¹ taken separately are selected from the group consisting of Cι-C₁₂ alkyl, Cι-C₁₂ cycloalkyl, and aryl; or R³⁰ and R³¹ taken together with the nitrogen atom form a saturated nitrogen heterocycle; and 9 •

    R is a phosphite ester protecting group.

    39
64. 64. The phosphoramidite compound of claim 64 wherein R is selected from the group consisting of methyl, 2-cyanoethyl, and 2-(4-nitrophenyl)ethyl.
65. 65. The phosphoramidite compound ofclaim 63 wherein R³⁰ and R³¹ are each isopropyl.
66. 66. The phosphoramidite compound of claim 63 wherein R³⁰ and R³¹ taken together is morpholino.
67. 67. The phosphoramidite compound of claim 63 wherein L is C₁-C₁₂ alkyldiyl.
68. 68. The phosphoramidite compound of claim 63 wherein L is attached at R 18 or R¹⁹ of DYE having the structure:
69. 69. The phosphoramidite compound of claim 68 having the structure:
70. 70. The phosphoramidite compound of claim 63 wherein L is

    O (CH₂CH₂O)n— CH₂CH₂-NH— C — and n ranges from 1 to 10.
71. 71. A method of forming a labelled substrate comprising the step of reacting a subsfrate selected from the group consisting of a polynucleotide, a nucleotide, a nucleoside, a polypeptide, a carbohydrate, a ligand, a substantially enantiomerically pure compound, a particle, and a surface, with the linking moiety ofthe compound ofclaim 5 or the energy-transfer dye ofclaim 31, whereby a labelled substrate is formed.
72. 72. The method of claim 71 wherein the linking moiety is N- hydroxysuccinimide.
73. 73. The method of claim 71 wherein the linking moiety is a phosphoramidite.
74. 74. The method ofclaim 71 wherein the substrate is substantially enantiomerically pure.
75. 75. The method ofclaim 71 wherein the substantially enantiomerically pure compound is (+) menthyl chloroformate or (-) menthyl chloroformate.
76. 76. The method ofclaim 71 wherein the labelled substrate comprises C-l 1 aminomethyl, C-l 9 carboxyl fluorescein having the structure:
77. 77. The method ofclaim 71 wherein the particle is a nanoparticle, a microsphere, a bead, or a liposome.
78. 78. The method ofclaim 71 wherein the surface is glass.
79. 79. A method of synthesizing a labelled polynucleotide comprising the step of coupling the phosphoramidite compound ofclaim 63 to a polynucleotide, wherein the polynucleotide is bound to a solid support, whereby a labelled polynucleotide is formed.
80. 80. A method of separating atropisomers of a C- 11 aminomethyl, C- 19 carboxyl fluorescein compound comprising the steps of:

    (a) reacting a C-l 1 aminomethyl, C-l 9 carboxyl fluorescein with a substantially pure enantiomer of an active ester or carboxylic acid to form diastereomeric carbamates;

    (b) separating the diastereomeric carbamates; and (c) hydrolyzing the separated diastereomers with aqueous acid.
81. 81. The method of claim 80 wherein the active ester is menthyl chloroformate.
82. 82. The method ofclaim 80 wherein the diastereomeric carbamates are separated by reverse-phase HPLC.
83. 83. A method of separating a mixture of labelled substrates wherein the labelled substrates are comprised of a compound ofclaim 1 or an energy-transfer dye of claim 31 , comprising the steps of:

    (a) separating a mixture of labelled subsfrates by electrophoresis; and

    (b) detecting the labelled subsfrates by fluorescence detection.
84. 84. The method ofclaim 83 wherein the labelled substrates are labelled polynucleotides.
85. 85. A method of separating a mixture of labelled substrates wherein the labelled substrates are comprised of a compound ofclaim 1 or an energy-transfer dye of claim 31, comprising the steps of:

    (a) separating a mixture of labelled substrates by chromatography; and

    (b) detecting the labelled substrates by fluorescence detection.
86. 86. A method of generating a labelled primer extension product, comprising the step of extending a primer-target hybrid with an enzymatically-incorporatable nucleotide, wherein said primer or said nucleotide is labelled with a compound according to claim 1 or an energy-transfer compound ofclaim 31, whereby the primer is extended.
87. 87. The method ofclaim 86 wherein the nucleotide is enzymatically- extendable.
88. 88. The method of claim 86 wherein the primer is a labelled polynucleotide comprising the formula:

    wherein DYE is a substantially pure atropisomer of a xanthene compound or an energy-transfer dye; B is a nucleobase; X is O, NH, or S; L is a linker;

    R²⁷ is H, OH, halide, azide, amine, alkylamine, Cι-C₆ alkyl, allyl, Cι-C₆ alkoxy, OCH₃, or OCH₂CH=CH₂; and R²⁸ is internucleotide phosphodiester or internucleotide analog; wherein the polynucleotide comprises 2 to 100 nucleotides.
89. 89. The method ofclaim 88 wherein B is selected from the group consisting of uracil, thymine, cytosine, adenine, 7-deazaadenine, guanine, and 7-deazaguanosine.
90. 90. The method ofclaim 86 wherein the enzymatically-incorporatable nucleotide is a labelled nucleoside or nucleotide having the formula: >

    wherein DYE is a substantially pure atropisomer of a xanthene compound or an energy-transfer dye; B is a nucleobase; L is a linker;

    R²⁵ is H, monophosphate, diphosphate, triphosphate, thiophosphate, or phosphate analog; and

    R²⁶ and R²⁷, when taken alone, are each independently H, HO, and F.
91. 91. The method of claim 86 further comprising a terminator nucleotide.
92. 92. The method of claim 90 wherein R²⁶ and R²⁷, when taken alone, are each independently H, F, a moiety which blocks polymerase-mediated target-directed primer extension, or when taken together form 2'-3'-didehydroribose.
93. 93. A method of polynucleotide sequencing comprising the steps of: forming a mixture of a first, a second, a third, and a fourth class of polynucleotides such that: each polynucleotide in the first class includes a 3 '-terminal dideoxyadenosine and is labelled with a first dye; each polynucleotide in the second class includes a 3 '-terminal dideoxycytidine and is labelled with a second dye; each polynucleotide in the third class includes a 3 '-terminal dideoxyguanosine and is labelled with a third dye; and each polynucleotide in the fourth class includes a 3 '-terminal dideoxythymidine and is labelled with a fourth dye; wherein one ofthe first, second, third, or fourth dyes is a compound according to claim 1 or an energy-transfer dye ofclaim 38, the other dyes are spectrally resolvable from each other; and separating the polynucleotides on the basis of size.
94. 94. The method of claim 93 further comprising the step of detecting the separated polynucleotides by fluorescence detection.
95. 95. The method ofclaim 93 further comprising the step of identifying the 3'- terminal nucleotide ofthe polynucleotides by the fluorescence spectrum ofthe dyes.
96. 96. A method of oligonucleotide ligation, comprising the steps of annealing two probes to a target sequence and forming a phosphodiester bond between the 5' terminus of one probe and the 3' terminus ofthe other probe; wherein one or both probes are labelled with a compound according to claim 1 or an energy-transfer dye ofclaim 31.
97. 97. A method of fragment analysis comprising the steps of separating labelled polynucleotide fragments by a size-dependent separation process, and detecting the separated labelled polynucleotide fragments subsequent to the separation process; wherein the fragments are labelled with a compound ofclaim 1 or an energy- transfer dye ofclaim 31.
98. 98. The method ofclaim 97 wherein the fragments are labelled with a mobility-modifying label.
99. 99. The method of claim 97 wherein the fragments are formed by ligation.
100. 100. The method of claim 97 wherein the size-dependent separation process is electrophoresis and the labelled polynucleotide fragments are detected by fluorescence.
101. 101. A method of amplification comprising the steps of annealing two or more primers to a target polynucleotide and extending the primers by a polymerase and a mixture of enzymatically-extendable nucleotides; wherein at least one ofthe primers is a labelled polynucleotide according to claim 55.
102. 102. A method of amplification comprising the steps of annealing two or more primers to a target polynucleotide and extending the primers by a polymerase and a mixture of enzymatically-extendable nucleotides; wherein at least one ofthe nucleotides is a labelled nucleotide according to claim 48.
103. 103. A method of amplification comprising the steps of annealing two or more primers and a fluorescent dye-quencher probe to a target nucleic acid and extending the primers by polymerase and a mixture of enzymatically-extendable nucleotides; wherein the probe is a labelled polynucleotide according to claim 55.
104. 104. A kit for labelling a polynucleotide, comprising a compound including a linking moiety according to claim 4 and a polynucleotide.
105. 105. A kit for labelling a polynucleotide, comprising a energy-transfer dye according to claim 31 and a polynucleotide.
106. 106. A kit for labelling a polynucleotide, comprising a phosphoramidite compound according to claim 63 and a polynucleotide.
107. 107. A kit for generating a labelled primer extension product, comprising one or more enzymatically-incorporatable nucleotides and a primer, wherein said primer is a labelled polynucleotide according to claim 55.
108. 108. A kit for generating a labelled primer extension product, comprising one or more enzymatically-incorporatable nucleotides and a primer, wherein at least one nucleotide is a labelled nucleotide according to claim 48.
109. 109. The kit of claim 108 wherein the labelled nucleotide is a terminator.
110. 110. The kit of claim 109 which comprises four different terminators, one which terminates at a target A, one which terminates at a target G, one which terminates at a target C and one which terminates at a target T or U.