CA2394392C - Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus - Google Patents

Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus Download PDF

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Publication number
CA2394392C
CA2394392C CA2394392A CA2394392A CA2394392C CA 2394392 C CA2394392 C CA 2394392C CA 2394392 A CA2394392 A CA 2394392A CA 2394392 A CA2394392 A CA 2394392A CA 2394392 C CA2394392 C CA 2394392C
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CA
Canada
Prior art keywords
ligand
rna
process according
labeling
antiligand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2394392A
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English (en)
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CA2394392A1 (fr
Inventor
Ali Laayoun
Duc Do
Charles Garret Miyada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Affymetrix Inc
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Biomerieux SA
Affymetrix Inc
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Publication date
Application filed by Biomerieux SA, Affymetrix Inc filed Critical Biomerieux SA
Publication of CA2394392A1 publication Critical patent/CA2394392A1/fr
Application granted granted Critical
Publication of CA2394392C publication Critical patent/CA2394392C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Inks, Pencil-Leads, Or Crayons (AREA)

Abstract

La présente invention concerne un procédé de marquage avec amplification de signal d'un acide ribonucléique (ARN), consistant à fragmenter l'ARN, à fixer un premier ligand au phosphate terminal situé à l'extrémité 3' end et/ou à l'extrémité 5' de chaque fragment dudit ARN, ledit phosphate terminal ayant été libéré lors de la fragmentation, et à fixer un agent de marquage au premier ligand. L'invention est, de préférence, utilisée dans le domaine du diagnostic médical.
CA2394392A 1999-12-17 1999-12-17 Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus Expired - Lifetime CA2394392C (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB1999/002072 WO2001044506A1 (fr) 1999-12-17 1999-12-17 Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus

Publications (2)

Publication Number Publication Date
CA2394392A1 CA2394392A1 (fr) 2001-06-21
CA2394392C true CA2394392C (fr) 2010-11-02

Family

ID=11004951

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2394392A Expired - Lifetime CA2394392C (fr) 1999-12-17 1999-12-17 Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus

Country Status (10)

Country Link
EP (1) EP1238115B1 (fr)
JP (1) JP2003516762A (fr)
CN (1) CN1166784C (fr)
AT (1) ATE445713T1 (fr)
AU (1) AU785085B2 (fr)
CA (1) CA2394392C (fr)
DE (1) DE69941546D1 (fr)
ES (1) ES2334471T3 (fr)
HK (1) HK1050552A1 (fr)
WO (1) WO2001044506A1 (fr)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7060441B2 (en) 2001-05-04 2006-06-13 Biomerieux Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling
US7338805B2 (en) 2001-05-04 2008-03-04 Bio Merieux Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules
FR2864550B1 (fr) 2003-12-29 2006-02-24 Commissariat Energie Atomique Puce d'analyse avec gamme etalon, trousses et procedes d'analyse.
FR2868071B1 (fr) 2004-03-26 2006-06-09 Biomerieux Sa Reactifs de marquage, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
FR2881437B1 (fr) 2005-01-31 2010-11-19 Biomerieux Sa Procede pour le diagnostic/pronostic d'un syndrome septique
FR2886735B1 (fr) 2005-06-01 2015-09-11 Biomerieux Sa Procede de marquage ou de traitement d'un echantillon biologique contenant des molecules biologiques d'interet, notamment des acides nucleiques
WO2007064022A1 (fr) * 2005-11-29 2007-06-07 Mitsubishi Rayon Co., Ltd. Procede pour confirmation de preparation d'acide nucleique
FR2917090B1 (fr) 2007-06-11 2012-06-15 Biomerieux Sa Reactifs de marquage portant des fonctions diazo et nitro, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
FR2934595B1 (fr) 2008-07-29 2013-04-05 Biomerieux Sa Reactifs de marquage ayant un noyau pyridine portant une fonction diazomethyle, procedes de synthese de tels reactifs et procedes de detection de molecules biologiques
WO2011150976A1 (fr) 2010-06-04 2011-12-08 bioMérieux Procédé et kit pour le pronostic du cancer colorectal
US9110079B2 (en) 2010-09-29 2015-08-18 Biomerieux Method and kit for establishing an in vitro prognosis on a patient exhibiting SIRS
WO2012129758A1 (fr) 2011-03-25 2012-10-04 Biomerieux Procédé et kit pour déterminer in vitro la probabilité qu'un individu soit atteint d'un cancer colorectal
CN105372350B (zh) * 2015-12-07 2017-10-31 合肥今越制药有限公司 一种低糖型强力枇杷露指纹图谱控制方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5317098A (en) * 1986-03-17 1994-05-31 Hiroaki Shizuya Non-radioisotope tagging of fragments
US4987071A (en) * 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
DE3910151A1 (de) * 1989-03-29 1990-10-04 Toni Dr Lindl 5'-bromo-2'-desoxyuridinmarkierte dna- oder rna- sonden
JP3208427B2 (ja) * 1989-09-29 2001-09-10 オー・エス・アイ・ファーマシューテイカルズ・インコーポレイテッド ヒトの生物学的流体中のｎｅｕ関連タンパク質の検出及び定量
US5858650A (en) * 1992-04-03 1999-01-12 Abbott Laboratories Methods for inactivating nucleotide sequences and metal chelates for use therein
FR2706618B1 (fr) * 1993-06-11 1995-09-01 Bio Merieux Dispositif pour le dosage d'haptènes et son utilisation.
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5834198A (en) * 1996-03-21 1998-11-10 Boehringer Mamnnheim Gmbh Selective photoinducted flavin-dependent cleavage of RNA at G-U base pairs and kits therefor
US5905024A (en) * 1996-12-17 1999-05-18 University Of Chicago Method for performing site-specific affinity fractionation for use in DNA sequencing
FR2780059B1 (fr) * 1998-06-17 2002-10-11 Bio Merieux Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus

Also Published As

Publication number Publication date
DE69941546D1 (de) 2009-11-26
CN1391616A (zh) 2003-01-15
HK1050552A1 (zh) 2003-06-27
AU1791200A (en) 2001-06-25
JP2003516762A (ja) 2003-05-20
CA2394392A1 (fr) 2001-06-21
EP1238115B1 (fr) 2009-10-14
EP1238115A1 (fr) 2002-09-11
WO2001044506A1 (fr) 2001-06-21
ES2334471T3 (es) 2010-03-10
ATE445713T1 (de) 2009-10-15
CN1166784C (zh) 2004-09-15
AU785085B2 (en) 2006-09-14

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Effective date: 20191217