CA2122734A1 - Dosage par amplification de restriction - Google Patents

Dosage par amplification de restriction

Info

Publication number
CA2122734A1
CA2122734A1 CA 2122734 CA2122734A CA2122734A1 CA 2122734 A1 CA2122734 A1 CA 2122734A1 CA 2122734 CA2122734 CA 2122734 CA 2122734 A CA2122734 A CA 2122734A CA 2122734 A1 CA2122734 A1 CA 2122734A1
Authority
CA
Canada
Prior art keywords
oligonucleotide
target
tris
sequence
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2122734
Other languages
English (en)
Inventor
Albert L. George, Jr.
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oncor Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2122734A1 publication Critical patent/CA2122734A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA 2122734 1991-11-12 1992-11-12 Dosage par amplification de restriction Abandoned CA2122734A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79017491A 1991-11-12 1991-11-12
US07/790,174 1991-11-12

Publications (1)

Publication Number Publication Date
CA2122734A1 true CA2122734A1 (fr) 1993-05-27

Family

ID=25149854

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2122734 Abandoned CA2122734A1 (fr) 1991-11-12 1992-11-12 Dosage par amplification de restriction

Country Status (4)

Country Link
EP (1) EP0620861A4 (fr)
JP (1) JPH07505760A (fr)
CA (1) CA2122734A1 (fr)
WO (1) WO1993010264A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5747255A (en) * 1995-09-29 1998-05-05 Lynx Therapeutics, Inc. Polynucleotide detection by isothermal amplification using cleavable oligonucleotides
KR20000025029A (ko) * 1998-10-07 2000-05-06 박한오 제한효소 절편의 배열화된 증폭의 전개방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4935357A (en) * 1986-02-05 1990-06-19 New England Biolabs, Inc. Universal restriction endonuclease
US5102784A (en) * 1990-05-04 1992-04-07 Oncor, Inc. Restriction amplification assay

Also Published As

Publication number Publication date
WO1993010264A1 (fr) 1993-05-27
EP0620861A1 (fr) 1994-10-26
EP0620861A4 (fr) 1995-07-19
JPH07505760A (ja) 1995-06-29

Similar Documents

Publication Publication Date Title
US5102784A (en) Restriction amplification assay
US11254970B2 (en) Detecting nucleic acid
EP1062367B1 (fr) Methodes de detection d'acide nucleique utilisant des zymogenes et des kits associees
EP0167238B1 (fr) Procédé d'analyse des polynucléotides par déplacement et réactif des complexes polynucléotidiques à cet effet
US5093245A (en) Labeling by simultaneous ligation and restriction
US6165714A (en) Devices and methods for detecting nucleic acid analytes in samples
JP4494630B2 (ja) 中型縦列繰り返しｄｎａマーカーを特定し分析するための材料と方法
US20070059690A1 (en) "Met/fret based method of target nucleic acid detection whereby the donor/acceptor moieties are on complementary strands"
Iovannisci et al. Ligation amplification and fluorescence detection of Mycobacterium tuberculosis DNA
WO2008109945A1 (fr) Analyse d'acide ribonucléique
US5451502A (en) Restriction amplification assay
Gillespie The magic and challenge of DNA probes as diagnostic reagents
CA2122734A1 (fr) Dosage par amplification de restriction
WO2022126721A1 (fr) Procédé de détection à haute spécificité d'une séquence d'acide nucléique cible
US6492120B1 (en) Nucleic acid hybridization assay utilizing tricyclic target and signal amplification
EP2561098B1 (fr) Détection d'acides nucléiques à médiation par une enzyme captive
JP4644944B2 (ja) 標的核酸検出法およびそのための試薬
WO2024062126A1 (fr) Procédé de détection d'une séquence d'acide nucléique cible
AU1523799A (en) Methods and compositions for detection of specific nucleotide sequences
US20080311568A1 (en) Compositions and Methods for Nucleic Acid Analysis of Sequences with Insertions or Deletions

Legal Events

Date Code Title Description
FZDE Dead