CA2383790C - Telomere restoration and extension of cell life-span in animals cloned from senescent somatic cells - Google Patents
Telomere restoration and extension of cell life-span in animals cloned from senescent somatic cells Download PDFInfo
- Publication number
- CA2383790C CA2383790C CA2383790A CA2383790A CA2383790C CA 2383790 C CA2383790 C CA 2383790C CA 2383790 A CA2383790 A CA 2383790A CA 2383790 A CA2383790 A CA 2383790A CA 2383790 C CA2383790 C CA 2383790C
- Authority
- CA
- Canada
- Prior art keywords
- cells
- cell
- cloned
- senescent
- telomerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241001465754 Metazoa Species 0.000 title claims abstract description 95
- 108091035539 telomere Proteins 0.000 title claims description 112
- 102000055501 telomere Human genes 0.000 title claims description 112
- 210000003411 telomere Anatomy 0.000 title claims description 106
- 210000004027 cell Anatomy 0.000 title abstract description 415
- 210000001082 somatic cell Anatomy 0.000 title abstract description 57
- 238000000034 method Methods 0.000 claims abstract description 98
- 230000000694 effects Effects 0.000 claims abstract description 54
- 238000010367 cloning Methods 0.000 claims abstract description 22
- 101001073422 Homo sapiens Pigment epithelium-derived factor Proteins 0.000 claims abstract 3
- 102100035846 Pigment epithelium-derived factor Human genes 0.000 claims abstract 3
- 238000012546 transfer Methods 0.000 claims description 69
- 210000002950 fibroblast Anatomy 0.000 claims description 48
- 210000003754 fetus Anatomy 0.000 claims description 38
- 241000283690 Bos taurus Species 0.000 claims description 33
- 210000001161 mammalian embryo Anatomy 0.000 claims description 25
- 210000000287 oocyte Anatomy 0.000 claims description 24
- 210000004940 nucleus Anatomy 0.000 claims description 23
- 108020004414 DNA Proteins 0.000 claims description 21
- 238000005516 engineering process Methods 0.000 claims description 11
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 10
- 230000012010 growth Effects 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 108020004511 Recombinant DNA Proteins 0.000 claims 3
- 108010017842 Telomerase Proteins 0.000 abstract description 100
- 108090000623 proteins and genes Proteins 0.000 abstract description 64
- 230000003716 rejuvenation Effects 0.000 abstract description 24
- 241000124008 Mammalia Species 0.000 abstract description 17
- 230000032677 cell aging Effects 0.000 abstract description 10
- 238000002054 transplantation Methods 0.000 abstract description 10
- 230000037361 pathway Effects 0.000 abstract description 7
- 230000035755 proliferation Effects 0.000 abstract description 5
- 230000003213 activating effect Effects 0.000 abstract description 4
- 230000009758 senescence Effects 0.000 description 44
- 210000001519 tissue Anatomy 0.000 description 35
- 230000014509 gene expression Effects 0.000 description 30
- 108090000102 pigment epithelium-derived factor Proteins 0.000 description 21
- 230000001965 increasing effect Effects 0.000 description 17
- 210000000349 chromosome Anatomy 0.000 description 15
- 206010043276 Teratoma Diseases 0.000 description 14
- 230000032683 aging Effects 0.000 description 14
- 230000002068 genetic effect Effects 0.000 description 14
- 210000002257 embryonic structure Anatomy 0.000 description 13
- 102000004169 proteins and genes Human genes 0.000 description 13
- 238000004458 analytical method Methods 0.000 description 12
- 244000309466 calf Species 0.000 description 12
- 230000022131 cell cycle Effects 0.000 description 11
- 150000001875 compounds Chemical class 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 11
- 210000000056 organ Anatomy 0.000 description 11
- 235000013601 eggs Nutrition 0.000 description 9
- 238000010353 genetic engineering Methods 0.000 description 9
- 210000004602 germ cell Anatomy 0.000 description 9
- 108020004999 messenger RNA Proteins 0.000 description 9
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 8
- 206010028980 Neoplasm Diseases 0.000 description 8
- 230000004913 activation Effects 0.000 description 8
- 238000003556 assay Methods 0.000 description 8
- 230000033228 biological regulation Effects 0.000 description 8
- 201000011510 cancer Diseases 0.000 description 8
- 238000012239 gene modification Methods 0.000 description 8
- 230000005017 genetic modification Effects 0.000 description 8
- 235000013617 genetically modified food Nutrition 0.000 description 8
- 230000004044 response Effects 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 7
- 108700019146 Transgenes Proteins 0.000 description 7
- 210000002459 blastocyst Anatomy 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 210000002919 epithelial cell Anatomy 0.000 description 7
- 239000003550 marker Substances 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 230000002062 proliferating effect Effects 0.000 description 7
- 238000004904 shortening Methods 0.000 description 7
- 230000008901 benefit Effects 0.000 description 6
- 230000004663 cell proliferation Effects 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 239000002299 complementary DNA Substances 0.000 description 6
- 230000001419 dependent effect Effects 0.000 description 6
- 230000002500 effect on skin Effects 0.000 description 6
- 210000005260 human cell Anatomy 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 6
- 238000001727 in vivo Methods 0.000 description 6
- 230000007246 mechanism Effects 0.000 description 6
- 201000006938 muscular dystrophy Diseases 0.000 description 6
- 230000035935 pregnancy Effects 0.000 description 6
- 230000001105 regulatory effect Effects 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 238000012216 screening Methods 0.000 description 6
- 238000001890 transfection Methods 0.000 description 6
- 230000009261 transgenic effect Effects 0.000 description 6
- 238000011282 treatment Methods 0.000 description 6
- 241001494479 Pecora Species 0.000 description 5
- 230000003247 decreasing effect Effects 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 5
- 230000001605 fetal effect Effects 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 231100000118 genetic alteration Toxicity 0.000 description 5
- 230000004077 genetic alteration Effects 0.000 description 5
- 210000001654 germ layer Anatomy 0.000 description 5
- 230000000394 mitotic effect Effects 0.000 description 5
- 230000008943 replicative senescence Effects 0.000 description 5
- 230000000392 somatic effect Effects 0.000 description 5
- 230000001225 therapeutic effect Effects 0.000 description 5
- 230000012746 DNA damage checkpoint Effects 0.000 description 4
- 206010016654 Fibrosis Diseases 0.000 description 4
- 108010033737 Pokeweed Mitogens Proteins 0.000 description 4
- 210000001744 T-lymphocyte Anatomy 0.000 description 4
- 238000010171 animal model Methods 0.000 description 4
- 210000003719 b-lymphocyte Anatomy 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 210000000601 blood cell Anatomy 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 208000019425 cirrhosis of liver Diseases 0.000 description 4
- 230000001086 cytosolic effect Effects 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 210000002308 embryonic cell Anatomy 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 4
- 230000028993 immune response Effects 0.000 description 4
- 239000003226 mitogen Substances 0.000 description 4
- 230000001459 mortal effect Effects 0.000 description 4
- 230000008439 repair process Effects 0.000 description 4
- 210000000130 stem cell Anatomy 0.000 description 4
- 230000003827 upregulation Effects 0.000 description 4
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 3
- 102000029816 Collagenase Human genes 0.000 description 3
- 108060005980 Collagenase Proteins 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 108060002716 Exonuclease Proteins 0.000 description 3
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 3
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 230000018199 S phase Effects 0.000 description 3
- 102000007316 Telomeric Repeat Binding Protein 2 Human genes 0.000 description 3
- 108010033710 Telomeric Repeat Binding Protein 2 Proteins 0.000 description 3
- 239000011543 agarose gel Substances 0.000 description 3
- 206010064930 age-related macular degeneration Diseases 0.000 description 3
- 210000004671 cell-free system Anatomy 0.000 description 3
- 230000007882 cirrhosis Effects 0.000 description 3
- 229960002424 collagenase Drugs 0.000 description 3
- 210000004748 cultured cell Anatomy 0.000 description 3
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 210000003981 ectoderm Anatomy 0.000 description 3
- 230000013020 embryo development Effects 0.000 description 3
- 210000001900 endoderm Anatomy 0.000 description 3
- 102000013165 exonuclease Human genes 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- 238000003209 gene knockout Methods 0.000 description 3
- 239000005090 green fluorescent protein Substances 0.000 description 3
- 238000009396 hybridization Methods 0.000 description 3
- 210000002865 immune cell Anatomy 0.000 description 3
- 210000002510 keratinocyte Anatomy 0.000 description 3
- 208000002780 macular degeneration Diseases 0.000 description 3
- 210000004962 mammalian cell Anatomy 0.000 description 3
- 210000003716 mesoderm Anatomy 0.000 description 3
- 210000002569 neuron Anatomy 0.000 description 3
- 201000008482 osteoarthritis Diseases 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 230000003362 replicative effect Effects 0.000 description 3
- 210000003491 skin Anatomy 0.000 description 3
- 238000002560 therapeutic procedure Methods 0.000 description 3
- 208000030507 AIDS Diseases 0.000 description 2
- 208000024827 Alzheimer disease Diseases 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 2
- 241000271566 Aves Species 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 208000002177 Cataract Diseases 0.000 description 2
- 206010014561 Emphysema Diseases 0.000 description 2
- 208000007514 Herpes zoster Diseases 0.000 description 2
- 206010020772 Hypertension Diseases 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- WGZDBVOTUVNQFP-UHFFFAOYSA-N N-(1-phthalazinylamino)carbamic acid ethyl ester Chemical compound C1=CC=C2C(NNC(=O)OCC)=NN=CC2=C1 WGZDBVOTUVNQFP-UHFFFAOYSA-N 0.000 description 2
- 208000036830 Normal foetus Diseases 0.000 description 2
- 108091093105 Nuclear DNA Proteins 0.000 description 2
- 108700020796 Oncogene Proteins 0.000 description 2
- 208000001132 Osteoporosis Diseases 0.000 description 2
- 208000018737 Parkinson disease Diseases 0.000 description 2
- 108091093037 Peptide nucleic acid Proteins 0.000 description 2
- 102100024553 Telomerase protein component 1 Human genes 0.000 description 2
- 101710169579 Telomerase protein component 1 Proteins 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 238000003149 assay kit Methods 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000003822 cell turnover Effects 0.000 description 2
- 208000029078 coronary artery disease Diseases 0.000 description 2
- 230000001351 cycling effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000002939 deleterious effect Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 230000003828 downregulation Effects 0.000 description 2
- 238000001493 electron microscopy Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 231100000024 genotoxic Toxicity 0.000 description 2
- 230000001738 genotoxic effect Effects 0.000 description 2
- LEQAOMBKQFMDFZ-UHFFFAOYSA-N glyoxal Chemical compound O=CC=O LEQAOMBKQFMDFZ-UHFFFAOYSA-N 0.000 description 2
- 210000003494 hepatocyte Anatomy 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000009545 invasion Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000002132 lysosomal effect Effects 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 210000001724 microfibril Anatomy 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 208000010125 myocardial infarction Diseases 0.000 description 2
- 208000015122 neurodegenerative disease Diseases 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 230000002028 premature Effects 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000008844 regulatory mechanism Effects 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 230000002207 retinal effect Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000014639 sexual reproduction Effects 0.000 description 2
- 230000019491 signal transduction Effects 0.000 description 2
- 230000009759 skin aging Effects 0.000 description 2
- 210000004927 skin cell Anatomy 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000008093 supporting effect Effects 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 2
- 238000010246 ultrastructural analysis Methods 0.000 description 2
- 210000004291 uterus Anatomy 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 238000010600 3H thymidine incorporation assay Methods 0.000 description 1
- RSWGJHLUYNHPMX-UHFFFAOYSA-N Abietic-Saeure Natural products C12CCC(C(C)C)=CC2=CCC2C1(C)CCCC2(C)C(O)=O RSWGJHLUYNHPMX-UHFFFAOYSA-N 0.000 description 1
- 102000006306 Antigen Receptors Human genes 0.000 description 1
- 108010083359 Antigen Receptors Proteins 0.000 description 1
- 208000023514 Barrett esophagus Diseases 0.000 description 1
- 208000023665 Barrett oesophagus Diseases 0.000 description 1
- 102000015081 Blood Coagulation Factors Human genes 0.000 description 1
- 108010039209 Blood Coagulation Factors Proteins 0.000 description 1
- 101100537096 Bos taurus ALYREF gene Proteins 0.000 description 1
- 101150013553 CD40 gene Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 208000037051 Chromosomal Instability Diseases 0.000 description 1
- 208000000094 Chronic Pain Diseases 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 206010010144 Completed suicide Diseases 0.000 description 1
- 108010062580 Concanavalin A Proteins 0.000 description 1
- 241000596148 Crocus Species 0.000 description 1
- 235000004237 Crocus Nutrition 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 101100016370 Danio rerio hsp90a.1 gene Proteins 0.000 description 1
- 101100285708 Dictyostelium discoideum hspD gene Proteins 0.000 description 1
- 206010013883 Dwarfism Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000555712 Forsythia Species 0.000 description 1
- 108010034791 Heterochromatin Proteins 0.000 description 1
- 241001640034 Heteropterys Species 0.000 description 1
- 208000023105 Huntington disease Diseases 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 102000004388 Interleukin-4 Human genes 0.000 description 1
- 108090000978 Interleukin-4 Proteins 0.000 description 1
- 102000011782 Keratins Human genes 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- 241000234435 Lilium Species 0.000 description 1
- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 102000005431 Molecular Chaperones Human genes 0.000 description 1
- 108010006519 Molecular Chaperones Proteins 0.000 description 1
- 208000019022 Mood disease Diseases 0.000 description 1
- YXOLAZRVSSWPPT-UHFFFAOYSA-N Morin Chemical compound OC1=CC(O)=CC=C1C1=C(O)C(=O)C2=C(O)C=C(O)C=C2O1 YXOLAZRVSSWPPT-UHFFFAOYSA-N 0.000 description 1
- 101000686985 Mouse mammary tumor virus (strain C3H) Protein PR73 Proteins 0.000 description 1
- 101100447665 Mus musculus Gas2 gene Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 102100038895 Myc proto-oncogene protein Human genes 0.000 description 1
- 101710135898 Myc proto-oncogene protein Proteins 0.000 description 1
- 241000234479 Narcissus Species 0.000 description 1
- 229930193140 Neomycin Natural products 0.000 description 1
- 101100257637 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) trf-2 gene Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 108010067035 Pancrelipase Proteins 0.000 description 1
- 241000009328 Perro Species 0.000 description 1
- 108090000430 Phosphatidylinositol 3-kinases Proteins 0.000 description 1
- 102000003993 Phosphatidylinositol 3-kinases Human genes 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 239000013616 RNA primer Substances 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 206010038687 Respiratory distress Diseases 0.000 description 1
- 108050002653 Retinoblastoma protein Proteins 0.000 description 1
- 108010081734 Ribonucleoproteins Proteins 0.000 description 1
- 102000004389 Ribonucleoproteins Human genes 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- KHPCPRHQVVSZAH-HUOMCSJISA-N Rosin Natural products O(C/C=C/c1ccccc1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 KHPCPRHQVVSZAH-HUOMCSJISA-N 0.000 description 1
- 238000011579 SCID mouse model Methods 0.000 description 1
- 101100071627 Schizosaccharomyces pombe (strain 972 / ATCC 24843) swo1 gene Proteins 0.000 description 1
- 206010040070 Septic Shock Diseases 0.000 description 1
- 206010040943 Skin Ulcer Diseases 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 230000006052 T cell proliferation Effects 0.000 description 1
- 210000000662 T-lymphocyte subset Anatomy 0.000 description 1
- 206010044248 Toxic shock syndrome Diseases 0.000 description 1
- 231100000650 Toxic shock syndrome Toxicity 0.000 description 1
- 206010052779 Transplant rejections Diseases 0.000 description 1
- 102100040245 Tumor necrosis factor receptor superfamily member 5 Human genes 0.000 description 1
- 102000013127 Vimentin Human genes 0.000 description 1
- 108010065472 Vimentin Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 206010002026 amyotrophic lateral sclerosis Diseases 0.000 description 1
- 238000004135 animal tissue culture Methods 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000003114 blood coagulation factor Substances 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000004413 cardiac myocyte Anatomy 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000025084 cell cycle arrest Effects 0.000 description 1
- 230000009744 cell cycle exit Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 230000002032 cellular defenses Effects 0.000 description 1
- 230000023715 cellular developmental process Effects 0.000 description 1
- 210000002230 centromere Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 210000001612 chondrocyte Anatomy 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 101150058580 cls-3 gene Proteins 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000024203 complement activation Effects 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 210000001771 cumulus cell Anatomy 0.000 description 1
- 229940043378 cyclin-dependent kinase inhibitor Drugs 0.000 description 1
- 230000021953 cytokinesis Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 210000004443 dendritic cell Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000008143 early embryonic development Effects 0.000 description 1
- 238000000635 electron micrograph Methods 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000009483 enzymatic pathway Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 206010015037 epilepsy Diseases 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 210000000232 gallbladder Anatomy 0.000 description 1
- 238000010363 gene targeting Methods 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 210000002980 germ line cell Anatomy 0.000 description 1
- 229940015043 glyoxal Drugs 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 210000000442 hair follicle cell Anatomy 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 210000002064 heart cell Anatomy 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 210000004458 heterochromatin Anatomy 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- -1 i.e. Proteins 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000006028 immune-suppresssive effect Effects 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 238000011532 immunohistochemical staining Methods 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000010874 in vitro model Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 229940028885 interleukin-4 Drugs 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 210000004153 islets of langerhan Anatomy 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 208000017169 kidney disease Diseases 0.000 description 1
- 210000002450 kidney nerve cell Anatomy 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 210000003712 lysosome Anatomy 0.000 description 1
- 230000001868 lysosomic effect Effects 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 1
- 235000007708 morin Nutrition 0.000 description 1
- 210000000472 morula Anatomy 0.000 description 1
- 201000006417 multiple sclerosis Diseases 0.000 description 1
- 210000003098 myoblast Anatomy 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 230000017066 negative regulation of growth Effects 0.000 description 1
- 229960004927 neomycin Drugs 0.000 description 1
- 210000003061 neural cell Anatomy 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 230000004770 neurodegeneration Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000011580 nude mouse model Methods 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 238000002135 phase contrast microscopy Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 210000001778 pluripotent stem cell Anatomy 0.000 description 1
- 206010036067 polydipsia Diseases 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000012743 protein tagging Effects 0.000 description 1
- 208000002815 pulmonary hypertension Diseases 0.000 description 1
- 238000009256 replacement therapy Methods 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 230000008672 reprogramming Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 210000001525 retina Anatomy 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 206010039073 rheumatoid arthritis Diseases 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 235000002020 sage Nutrition 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 210000004683 skeletal myoblast Anatomy 0.000 description 1
- 231100000019 skin ulcer Toxicity 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 238000010374 somatic cell nuclear transfer Methods 0.000 description 1
- 208000020431 spinal cord injury Diseases 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000003153 stable transfection Methods 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 231100000617 superantigen Toxicity 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 108010090575 telomere terminal transferase Proteins 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 208000037816 tissue injury Diseases 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- KHPCPRHQVVSZAH-UHFFFAOYSA-N trans-cinnamyl beta-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OCC=CC1=CC=CC=C1 KHPCPRHQVVSZAH-UHFFFAOYSA-N 0.000 description 1
- 230000037426 transcriptional repression Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 208000027930 type IV hypersensitivity disease Diseases 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000005167 vascular cell Anatomy 0.000 description 1
- 210000005048 vimentin Anatomy 0.000 description 1
- 230000036266 weeks of gestation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
- A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
- A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/08—Antiepileptics; Anticonvulsants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
- A61P25/16—Anti-Parkinson drugs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/18—Antivirals for RNA viruses for HIV
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
- A61P5/14—Drugs for disorders of the endocrine system of the thyroid hormones, e.g. T3, T4
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2517/00—Cells related to new breeds of animals
- C12N2517/04—Cells produced using nuclear transfer
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Biomedical Technology (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Physical Education & Sports Medicine (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Diabetes (AREA)
- Rheumatology (AREA)
- Endocrinology (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Psychology (AREA)
- Virology (AREA)
- Reproductive Health (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Pain & Pain Management (AREA)
- Emergency Medicine (AREA)
- Oncology (AREA)
- Vascular Medicine (AREA)
- AIDS & HIV (AREA)
- Obesity (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15234099P | 1999-09-07 | 1999-09-07 | |
| US60/152,340 | 1999-09-07 | ||
| US17948600P | 2000-02-01 | 2000-02-01 | |
| US60/179,486 | 2000-02-01 | ||
| US52702600A | 2000-03-16 | 2000-03-16 | |
| US09/527,026 | 2000-03-16 | ||
| US52087900A | 2000-04-05 | 2000-04-05 | |
| US09/520,879 | 2000-04-05 | ||
| PCT/US2000/024393 WO2001018236A1 (en) | 1999-09-07 | 2000-09-06 | Telomere restoration and extension of cell life-span in animals cloned from senescent somatic cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2383790A1 CA2383790A1 (en) | 2001-03-15 |
| CA2383790C true CA2383790C (en) | 2016-11-22 |
Family
ID=27496015
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2383790A Expired - Lifetime CA2383790C (en) | 1999-09-07 | 2000-09-06 | Telomere restoration and extension of cell life-span in animals cloned from senescent somatic cells |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP1214443A4 (https=) |
| JP (1) | JP2003508079A (https=) |
| CN (1) | CN1377424A (https=) |
| AU (1) | AU7350800A (https=) |
| BR (1) | BR0013844A (https=) |
| CA (1) | CA2383790C (https=) |
| IL (2) | IL148457A0 (https=) |
| MX (1) | MXPA02002443A (https=) |
| NZ (1) | NZ531844A (https=) |
| WO (1) | WO2001018236A1 (https=) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001018193A1 (en) * | 1999-09-07 | 2001-03-15 | Advanced Cell Technology, Inc. | Method for generating immune-compatible cells and tissues using nuclear transfer techniques |
| US7947457B2 (en) | 2007-01-08 | 2011-05-24 | The Invention Science Fund I, Llc | Systems for genome selection |
| US7709245B2 (en) | 2007-01-08 | 2010-05-04 | The Invention Science Fund I, Llc | Systems for genome selection |
| US8003371B2 (en) | 2007-01-08 | 2011-08-23 | The Invention Science Fund I, Llc | Systems for genome selection |
| US7718418B2 (en) | 2007-01-08 | 2010-05-18 | The Invention Science Fund I, Llc | Systems for genome selection |
| US7709244B2 (en) | 2007-01-08 | 2010-05-04 | The Invention Science Fund I, Llc | Systems for genome selection |
| US8012740B2 (en) | 2007-01-08 | 2011-09-06 | The Invention Science Fund I, Llc | Systems for genome selection |
| US7947455B2 (en) | 2007-01-08 | 2011-05-24 | The Invention Science Fund I, Llc | System for genome selection |
| US7985578B2 (en) | 2007-01-08 | 2011-07-26 | The Invention Science Fund I, Llc | Systems for genome selection |
| US8521440B2 (en) | 2007-01-08 | 2013-08-27 | The Invention Science Fund I, Llc | Systems for genome selection |
| US7648823B2 (en) | 2007-01-08 | 2010-01-19 | Searete Llc | Systems for genome selection |
| US7713728B2 (en) | 2007-01-08 | 2010-05-11 | The Invention Sciencefund I, Llc | Systems for genome selection |
| US20110086424A1 (en) * | 2008-05-06 | 2011-04-14 | Advanced Cell Technology, Inc. | Methods for producing enucleated erythroid cells derived from pluripotent stem cells |
| JP5888852B2 (ja) * | 2010-12-08 | 2016-03-22 | 学校法人近畿大学 | 免疫不全動物を用いた細胞の製法 |
| CN103562376B (zh) * | 2011-04-08 | 2017-12-29 | 国家医疗保健研究所 | 复壮细胞的方法 |
| EP3782628B1 (en) * | 2013-03-15 | 2024-08-07 | Elixirgen Therapeutics, Inc. | Methods of using zscan4 for rejuvenating human cells |
| SG10201907918VA (en) | 2015-03-23 | 2019-10-30 | Astellas Inst For Regenerative Medicine | Improved assays for potency of human retinal pigment epithelium (rpe) cells and photoreceptor progenitors |
| CN112654244B (zh) * | 2018-09-06 | 2023-07-25 | 德克萨斯大学系统董事会 | 端粒酶全酶复合体及其使用方法 |
| EP4525895A1 (en) * | 2022-05-17 | 2025-03-26 | National University of Singapore | Chimeric ovarian follicle-based therapy to treat female infertility |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5489508A (en) * | 1992-05-13 | 1996-02-06 | University Of Texas System Board Of Regents | Therapy and diagnosis of conditions related to telomere length and/or telomerase activity |
| US5648215A (en) * | 1992-05-13 | 1997-07-15 | Board Of Regents, The University Of Texas System | Telomerase diagnostic methods |
| US5945577A (en) * | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| AU745334B2 (en) * | 1997-03-06 | 2002-03-21 | Advanced Cell Technology, Inc. | Method of cloning animals |
| GB9809178D0 (en) * | 1998-04-29 | 1998-07-01 | Univ Edinburgh | Nuclear reprogramming of somatic cells |
| AU782286B2 (en) * | 1999-06-30 | 2005-07-14 | Advanced Cell Technology, Inc. | Cytoplasmic transfer to de-differentiate recipient cells |
| WO2001018193A1 (en) * | 1999-09-07 | 2001-03-15 | Advanced Cell Technology, Inc. | Method for generating immune-compatible cells and tissues using nuclear transfer techniques |
-
2000
- 2000-09-06 CN CN00813712A patent/CN1377424A/zh active Pending
- 2000-09-06 JP JP2001521771A patent/JP2003508079A/ja active Pending
- 2000-09-06 IL IL14845700A patent/IL148457A0/xx unknown
- 2000-09-06 MX MXPA02002443A patent/MXPA02002443A/es not_active Application Discontinuation
- 2000-09-06 AU AU73508/00A patent/AU7350800A/en not_active Abandoned
- 2000-09-06 EP EP00961569A patent/EP1214443A4/en not_active Ceased
- 2000-09-06 WO PCT/US2000/024393 patent/WO2001018236A1/en not_active Ceased
- 2000-09-06 BR BR0013844-4A patent/BR0013844A/pt not_active Application Discontinuation
- 2000-09-06 NZ NZ531844A patent/NZ531844A/en not_active IP Right Cessation
- 2000-09-06 CA CA2383790A patent/CA2383790C/en not_active Expired - Lifetime
-
2002
- 2002-02-28 IL IL148457A patent/IL148457A/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| WO2001018236A1 (en) | 2001-03-15 |
| NZ531844A (en) | 2005-08-26 |
| IL148457A (en) | 2013-01-31 |
| JP2003508079A (ja) | 2003-03-04 |
| EP1214443A4 (en) | 2003-08-27 |
| CA2383790A1 (en) | 2001-03-15 |
| AU7350800A (en) | 2001-04-10 |
| CN1377424A (zh) | 2002-10-30 |
| MXPA02002443A (es) | 2004-07-16 |
| IL148457A0 (en) | 2002-09-12 |
| BR0013844A (pt) | 2002-05-14 |
| EP1214443A1 (en) | 2002-06-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20040180430A1 (en) | Methods of restoring telomere length and extending cell lifespan using nuclear transfer | |
| CA2383790C (en) | Telomere restoration and extension of cell life-span in animals cloned from senescent somatic cells | |
| WO2001018236A9 (en) | Telomere restoration and extension of cell life-span in animals cloned from senescent somatic cells | |
| Marques-Mari et al. | Differentiation of germ cells and gametes from stem cells | |
| US20050210537A1 (en) | Pluripotent mammalian cells | |
| JPWO2005080598A1 (ja) | 体細胞核初期化物質のスクリーニング方法 | |
| JP2011188873A (ja) | 体細胞の遺伝的改変とそれらの使用 | |
| JP6980218B2 (ja) | Ｃａｓ９タンパク質を哺乳動物の受精卵に導入する方法 | |
| JP2019502400A (ja) | キメラ胚補助臓器作製用の組成物及び方法 | |
| EP2785832A1 (en) | Haploid cells | |
| Wilmut et al. | Embryonic and somatic cell cloning | |
| JP2002512787A (ja) | 幹細胞を得る方法 | |
| Loi et al. | Asymmetric nuclear reprogramming in somatic cell nuclear transfer? | |
| CN110283847A (zh) | 一种同时定点整合fad3和fabp4基因的载体及重组细胞 | |
| US20120196769A1 (en) | Methods of repairing tandemly repeated DNA sequences and extending cell life-span using nuclear transfer | |
| WO2001084920A1 (en) | The production of agricultural animals from embryonic stem (es) cells | |
| US20060041946A1 (en) | Nuclear transfer nuclei from histone hypomethylated donor cells | |
| AU2006252076A1 (en) | Telomere restoration and extension of cell life-span in animals cloned from senescent somatic cells | |
| KR102270151B1 (ko) | Pkd2 넉아웃 질환모델용 돼지 및 이의 용도 | |
| Georgiou et al. | In vitro spermatogenesis as a method to bypass pre‐meiotic or post‐meiotic barriers blocking the spermatogenetic process: genetic and epigenetic implications in assisted reproductive technology | |
| Miyashita et al. | Cloned cows with short telomeres deliver healthy offspring with normal-length telomeres | |
| Mezger et al. | Heat shock protein synthesis in preimplantation mouse embryos and embryonal carcinoma cells | |
| Zhang et al. | Deletion of RBM20 exon 9 impairs skeletal muscle growth and satellite cell function in pigs | |
| West | Embryonic stem cells: prospects of regenerative medicine for the treatment of human aging | |
| KR20020008398A (ko) | 핵 이식용 세포분열 말기의 세포핵 결실 난모세포 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20200908 |