CA2383423A1 - Reverse transfection method - Google Patents
Reverse transfection method Download PDFInfo
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- CA2383423A1 CA2383423A1 CA002383423A CA2383423A CA2383423A1 CA 2383423 A1 CA2383423 A1 CA 2383423A1 CA 002383423 A CA002383423 A CA 002383423A CA 2383423 A CA2383423 A CA 2383423A CA 2383423 A1 CA2383423 A1 CA 2383423A1
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- 229910052722 tritium Inorganic materials 0.000 description 1
- 102000047459 trkC Receptor Human genes 0.000 description 1
- 108010064892 trkC Receptor Proteins 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Laminated Bodies (AREA)
- Wrappers (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15473799P | 1999-09-17 | 1999-09-17 | |
| US60/154,737 | 1999-09-17 | ||
| US19358000P | 2000-03-30 | 2000-03-30 | |
| US60/193,580 | 2000-03-30 | ||
| PCT/US2000/025457 WO2001020015A1 (en) | 1999-09-17 | 2000-09-18 | Reverse transfection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2383423A1 true CA2383423A1 (en) | 2001-03-22 |
Family
ID=26851730
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002383423A Abandoned CA2383423A1 (en) | 1999-09-17 | 2000-09-18 | Reverse transfection method |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US6544790B1 (https=) |
| EP (1) | EP1218529B1 (https=) |
| JP (1) | JP2003509060A (https=) |
| AT (1) | ATE346156T1 (https=) |
| CA (1) | CA2383423A1 (https=) |
| DE (1) | DE60031997D1 (https=) |
| WO (1) | WO2001020015A1 (https=) |
Families Citing this family (104)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6515009B1 (en) | 1991-09-27 | 2003-02-04 | Neorx Corporation | Therapeutic inhibitor of vascular smooth muscle cells |
| US5811447A (en) | 1993-01-28 | 1998-09-22 | Neorx Corporation | Therapeutic inhibitor of vascular smooth muscle cells |
| US6143557A (en) * | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
| US6720140B1 (en) * | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
| US7351578B2 (en) | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
| US20030124555A1 (en) * | 2001-05-21 | 2003-07-03 | Invitrogen Corporation | Compositions and methods for use in isolation of nucleic acid molecules |
| EP1025217B1 (en) * | 1997-10-24 | 2006-10-04 | Invitrogen Corporation | Recombinational cloning using nucleic acids having recombination sites |
| US20050221280A1 (en) | 1998-02-02 | 2005-10-06 | Odyssey Thera, Inc. | Protein-protein interactions for pharmacological profiling |
| US6990221B2 (en) * | 1998-02-07 | 2006-01-24 | Biodiscovery, Inc. | Automated DNA array image segmentation and analysis |
| EP1173460B1 (en) * | 1999-03-02 | 2009-09-16 | Life Technologies Corporation | Compositions and methods for use in recombinational cloning of nucleic acids |
| US20020006664A1 (en) * | 1999-09-17 | 2002-01-17 | Sabatini David M. | Arrayed transfection method and uses related thereto |
| US6731781B1 (en) * | 1999-09-30 | 2004-05-04 | Biodiscovery, Inc. | System and method for automatically processing microarrays |
| US7099502B2 (en) * | 1999-10-12 | 2006-08-29 | Biodiscovery, Inc. | System and method for automatically processing microarrays |
| NZ539430A (en) * | 1999-12-10 | 2006-09-29 | Invitrogen Corp | Use of multiple recombination sites with unique specificity in recombinational cloning |
| US8143195B2 (en) * | 2000-01-24 | 2012-03-27 | Yingjian Wang | Arrays for bringing two or more reagents in contact with one or more biological targets and methods for making and using the arrays |
| US7244560B2 (en) * | 2000-05-21 | 2007-07-17 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| US7198924B2 (en) * | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| WO2002018537A2 (en) * | 2000-08-29 | 2002-03-07 | Yeda Research And Development Co. Ltd. | Methods of isolating genes encoding proteins of specific function and of screening for pharmaceutically active agents |
| US20050158702A1 (en) * | 2000-09-05 | 2005-07-21 | Stuelpnagel John R. | Cellular arrays comprising encoded cells |
| JP2004537712A (ja) | 2000-10-18 | 2004-12-16 | バーチャル・アレイズ・インコーポレーテッド | 多重細胞分析システム |
| US6902933B2 (en) * | 2000-11-03 | 2005-06-07 | Regents Of The University Of Michigan | Surface transfection and expression procedure |
| US6897067B2 (en) | 2000-11-03 | 2005-05-24 | Regents Of The University Of Michigan | Surface transfection and expression procedure |
| EP1330516B1 (en) * | 2000-11-03 | 2007-01-17 | The Regents Of The University Of Michigan | Surface transfection and expression procedure |
| US6890556B1 (en) * | 2001-02-14 | 2005-05-10 | Northwestern University | Controlled surface-associated delivery of genes and oligonucleotides |
| US7029697B2 (en) * | 2001-02-14 | 2006-04-18 | Northwestern University | Controlled surface-associated delivery of genes and oligonucleotides |
| US20030096414A1 (en) * | 2001-03-27 | 2003-05-22 | Invitrogen Corporation | Culture medium for cell growth and transfection |
| US20070111201A1 (en) * | 2001-04-30 | 2007-05-17 | Benjamin Doranz | Reverse transfection of cell arrays for structural and functional analyses of proteins |
| US6613083B2 (en) | 2001-05-02 | 2003-09-02 | Eckhard Alt | Stent device and method |
| AU2002303645A1 (en) * | 2001-05-04 | 2002-11-18 | Axiom Biotechnologies, Inc. | Matrix assays in genomically indexed cells |
| US6670129B2 (en) | 2001-09-24 | 2003-12-30 | Corning Incorporated | Cell transfection apparatus and methods for making and using the cell transfection apparatus |
| US20030124549A1 (en) * | 2001-10-11 | 2003-07-03 | Xerox Corporation | Devices and methods for detecting genetic sequences |
| AU2003213791A1 (en) * | 2002-03-05 | 2003-09-22 | Vitra Bioscience, Inc. | Multiplexed cell transfection using coded carriers |
| US20070054266A1 (en) * | 2002-05-28 | 2007-03-08 | National Institute Of Advanced Industrial Science And Technology | Chemical sensor system |
| JP2005528102A (ja) * | 2002-06-03 | 2005-09-22 | パムジーン ビー．ブイ． | 多用途の生存マイクロアレイを用いる、細胞に基づく高処理量アッセイのための方法 |
| US7105347B2 (en) | 2002-07-30 | 2006-09-12 | Corning Incorporated | Method and device for protein delivery into cells |
| JP3887588B2 (ja) * | 2002-08-30 | 2007-02-28 | 株式会社リガク | Ｘ線回折による応力測定法 |
| US7512496B2 (en) * | 2002-09-25 | 2009-03-31 | Soheil Shams | Apparatus, method, and computer program product for determining confidence measures and combined confidence measures for assessing the quality of microarrays |
| US7422853B1 (en) | 2002-10-04 | 2008-09-09 | Myriad Genetics, Inc. | RNA interference using a universal target |
| US6905878B2 (en) * | 2002-12-19 | 2005-06-14 | The Scripps Research Institute | DNA array for high throughput solid-phase transfection and method for producing the same |
| US20040138154A1 (en) | 2003-01-13 | 2004-07-15 | Lei Yu | Solid surface for biomolecule delivery and high-throughput assay |
| US20070269891A9 (en) * | 2003-01-13 | 2007-11-22 | Yasunobu Tanaka | Solid surface with immobilized degradable cationic polymer for transfecting eukaryotic cells |
| US7691580B2 (en) | 2003-01-29 | 2010-04-06 | Corning Incorporated | Reverse protein delivery into cells on coded microparticles |
| WO2005028615A2 (en) * | 2003-06-26 | 2005-03-31 | Invitrogen Corporation | Methods and compositions for detecting promoter activity and expressing fusion proteins |
| US7883720B2 (en) | 2003-07-09 | 2011-02-08 | Wisconsin Alumni Research Foundation | Charge-dynamic polymers and delivery of anionic compounds |
| AU2004261999B2 (en) * | 2003-08-01 | 2010-05-20 | Dynal Biotech Inc. | Self-hybridizing multiple target nucleic acid probes and methods of use |
| US7488451B2 (en) | 2003-09-15 | 2009-02-10 | Millipore Corporation | Systems for particle manipulation |
| WO2005054438A2 (en) | 2003-12-01 | 2005-06-16 | Invitrogen Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
| US7553631B2 (en) * | 2003-12-11 | 2009-06-30 | Beth Israel Deaconess Medical Center, Inc. | RBP4 in insulin sensitivity/resistance, diabetes, and obesity |
| WO2005078094A2 (en) * | 2004-02-06 | 2005-08-25 | Dharmacon, Inc. | Stabilized rnas as transfection controls and silencing reagents |
| US20090280567A1 (en) * | 2004-02-06 | 2009-11-12 | Dharmacon, Inc. | Stabilized sirnas as transfection controls and silencing reagents |
| US7125709B2 (en) * | 2004-02-10 | 2006-10-24 | Nitto Denko Corporation | Culture device and method for eukaryotic cell transfection |
| JP4551903B2 (ja) * | 2004-02-18 | 2010-09-29 | 日東電工株式会社 | 真核細胞トランスフェクションのための培養装置および方法 |
| KR101147147B1 (ko) * | 2004-04-01 | 2012-05-25 | 머크 샤프 앤드 돔 코포레이션 | Ｒｎａ 간섭의 오프 타겟 효과 감소를 위한 변형된폴리뉴클레오타이드 |
| GB0410105D0 (en) * | 2004-05-06 | 2004-06-09 | Amersham Biosciences Uk Ltd | Method for characterising compounds |
| US7935811B2 (en) * | 2004-11-22 | 2011-05-03 | Dharmacon, Inc. | Apparatus and system having dry gene silencing compositions |
| US7923207B2 (en) * | 2004-11-22 | 2011-04-12 | Dharmacon, Inc. | Apparatus and system having dry gene silencing pools |
| US20060166234A1 (en) * | 2004-11-22 | 2006-07-27 | Barbara Robertson | Apparatus and system having dry control gene silencing compositions |
| EP1814994A4 (en) * | 2004-11-22 | 2010-07-14 | Dharmacon Inc | APPARATUS AND SYSTEM COMPRISING DRY EXTINCTION DRY COMPOSITIONS |
| WO2006060646A2 (en) * | 2004-12-03 | 2006-06-08 | Board Of Regents, The University Of Texas System | Cell microarray for profiling of cellular phenotypes and gene function |
| EP1836293A2 (en) * | 2004-12-17 | 2007-09-26 | Nitto Denko Corporation | Immobilized degradable cationic polymer for transfecting eukaryotic cells |
| CA2596758A1 (en) * | 2005-02-03 | 2006-08-10 | King Faisal Specialist Hospital & Research Centre | Method of generating translationally active linear dna molecules and use thereof in array formats |
| US20060223777A1 (en) * | 2005-03-29 | 2006-10-05 | Dharmacon, Inc. | Highly functional short hairpin RNA |
| US20060257858A1 (en) * | 2005-04-01 | 2006-11-16 | Invitrogen Corporation | Methods and compositions for introducing biopolymers into cells |
| US8076090B2 (en) | 2005-04-05 | 2011-12-13 | Corning Incorporated | Label free biosensors and cells |
| US8734851B2 (en) * | 2005-04-29 | 2014-05-27 | Wisconsin Alumni Research Foundation | Localized delivery of nucleic acid by polyelectrolyte assemblies |
| US20090131271A1 (en) * | 2005-05-17 | 2009-05-21 | Kyushu University, National University Corporation | Method for delivering nucleic acid and device for delivering nucleic acid |
| CA2619139C (en) * | 2005-09-09 | 2013-06-25 | Jianhui Rong | Cell-based microarrays and methods of use |
| WO2007042771A1 (en) * | 2005-10-10 | 2007-04-19 | Astrazeneca Ab | Three hybrid screening assay using reverse transfected mammalian cell arrays |
| US20070161031A1 (en) * | 2005-12-16 | 2007-07-12 | The Board Of Trustees Of The Leland Stanford Junior University | Functional arrays for high throughput characterization of gene expression regulatory elements |
| AU2007299705B2 (en) * | 2006-09-22 | 2012-09-06 | Dharmacon, Inc. | Duplex oligonucleotide complexes and methods for gene silencing by RNA interference |
| US7687267B2 (en) * | 2006-09-30 | 2010-03-30 | Rational Biotechnology Inc. | High-throughput cell transfection device and methods of using thereof |
| EP2125220A1 (en) * | 2006-12-13 | 2009-12-02 | Qiagen GmbH | Transfection microarrays |
| US8834918B2 (en) * | 2007-01-22 | 2014-09-16 | Wisconsin Alumni Research Foundation | Modified multilayered film |
| EP2135080A4 (en) * | 2007-03-08 | 2010-12-01 | Switchgear Genomics | FUNCTIONAL NETWORKS FOR THE CHARACTERIZATION OF TREATMENT OF REGULATORY ELEMENTS IN NON-TRANSLATED REGIONS OF GENES |
| CA2697658A1 (en) * | 2007-08-24 | 2009-03-05 | Cytopathfinder, Inc. | Transfection device utilizing sericin |
| WO2009049100A2 (en) | 2007-10-09 | 2009-04-16 | Wisconsin Alumni Research Foundation | Ultrathin multilayered films for controlled release of anionic reagents |
| US7845686B2 (en) * | 2007-12-17 | 2010-12-07 | S & B Technical Products, Inc. | Restrained pipe joining system for plastic pipe |
| US8188060B2 (en) | 2008-02-11 | 2012-05-29 | Dharmacon, Inc. | Duplex oligonucleotides with enhanced functionality in gene regulation |
| US8324333B2 (en) | 2008-06-05 | 2012-12-04 | Wisconsin Alumni Research Foundation | Anionic charge-dynamic polymers for release of cationic agents |
| US8815779B2 (en) | 2009-09-16 | 2014-08-26 | SwitchGear Genomics, Inc. | Transcription biomarkers of biological responses and methods |
| CN101787375B (zh) * | 2010-02-10 | 2012-08-22 | 浙江大学 | 一种反向非病毒载体基因转染的方法 |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| US9029297B2 (en) * | 2011-07-25 | 2015-05-12 | Csir | Array printing |
| US9034797B2 (en) | 2011-07-25 | 2015-05-19 | Csir | Array printing |
| US9012375B2 (en) | 2011-07-25 | 2015-04-21 | Csir | Array printing |
| EP4592400A3 (en) | 2012-10-17 | 2025-10-29 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| CA2916662C (en) | 2013-06-25 | 2022-03-08 | Prognosys Biosciences, Inc. | Methods and systems for determining spatial patterns of biological targets in a sample |
| LT2981616T (lt) * | 2013-07-15 | 2018-11-26 | Universität Heidelberg | Baltymų ir nukleino rūgščių kietos fazės transfekavimas |
| US20170362588A1 (en) * | 2014-12-15 | 2017-12-21 | Novellusdx Ltd. | Systems, devices, kits and methods for indirect transfection of multiple sets of nucleic-acids and transfer of molecules |
| EP3901282B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| WO2020123316A2 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Methods for determining a location of a biological analyte in a biological sample |
| US12529094B2 (en) | 2018-12-10 | 2026-01-20 | 10X Genomics, Inc. | Imaging system hardware |
| US12157124B2 (en) | 2019-11-06 | 2024-12-03 | 10X Genomics, Inc. | Imaging system hardware |
| US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4421186B1 (en) | 2020-06-08 | 2025-08-13 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| WO2021252747A1 (en) | 2020-06-10 | 2021-12-16 | 1Ox Genomics, Inc. | Fluid delivery methods |
| WO2022061150A2 (en) | 2020-09-18 | 2022-03-24 | 10X Geonomics, Inc. | Sample handling apparatus and image registration methods |
| EP4729631A2 (en) | 2020-12-21 | 2026-04-22 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| WO2022256503A1 (en) | 2021-06-03 | 2022-12-08 | 10X Genomics, Inc. | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| WO2023102118A2 (en) | 2021-12-01 | 2023-06-08 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| WO2023122033A1 (en) | 2021-12-20 | 2023-06-29 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4591567A (en) | 1982-04-21 | 1986-05-27 | California Institute Of Technology | Recombinant DNA screening system including fixed array replicator and support |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US5807522A (en) * | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| JPH11505505A (ja) | 1994-06-27 | 1999-05-21 | ザ・ジョンズ・ホプキンス・ユニバーシティー | ターゲッティングされた遺伝子輸送システム |
| US5811274A (en) | 1994-12-09 | 1998-09-22 | The Regents Of The University Of Michigan | Methods, compositions and apparatus for cell transfection |
| JP3867160B2 (ja) * | 1995-07-03 | 2007-01-10 | 大日本住友製薬株式会社 | 遺伝子製剤 |
| US5851818A (en) | 1996-05-31 | 1998-12-22 | Sequus Pharmaceuticals, Inc. | Condensed plasmid-liposome complex for transfection |
| US6133026A (en) | 1996-05-31 | 2000-10-17 | Sequus Pharmaceuticals, Inc. | Condensed plasmid-liposome complex for transfection |
| AU3572799A (en) | 1998-04-24 | 1999-11-16 | Genova Pharmaceuticals Corporation | Function-based gene discovery |
| ATE282717T1 (de) | 1998-04-30 | 2004-12-15 | Max Planck Gesellschaft | Neuartiges verfahren zur identifizierung von klonen mit einer gewünschten biologischen eigenschaft, ausgehend von einer expressionsgenbank |
| EP1073771B1 (en) | 1998-04-30 | 2004-12-01 | Max-Planck-Gesellschaft Zur Förderung Der Wissenschaften E.V. | New method for the selection of clones of an expression library involving rearraying |
| GB9908681D0 (en) | 1999-04-16 | 1999-06-09 | Central Research Lab Ltd | Apparatus for, and method of, introducing a substance into an object |
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- 2000-09-18 DE DE60031997T patent/DE60031997D1/de not_active Expired - Lifetime
- 2000-09-18 AT AT00963550T patent/ATE346156T1/de not_active IP Right Cessation
- 2000-09-18 JP JP2001523786A patent/JP2003509060A/ja active Pending
- 2000-09-18 WO PCT/US2000/025457 patent/WO2001020015A1/en not_active Ceased
- 2000-09-18 EP EP00963550A patent/EP1218529B1/en not_active Expired - Lifetime
- 2000-09-18 US US09/664,297 patent/US6544790B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE60031997D1 (de) | 2007-01-04 |
| EP1218529B1 (en) | 2006-11-22 |
| WO2001020015A1 (en) | 2001-03-22 |
| EP1218529A1 (en) | 2002-07-03 |
| WO2001020015A9 (en) | 2002-10-03 |
| JP2003509060A (ja) | 2003-03-11 |
| US6544790B1 (en) | 2003-04-08 |
| ATE346156T1 (de) | 2006-12-15 |
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