CA2381666C - Method of producing an undifferentiated avian cell culture using avian primordial germ cells - Google Patents

Method of producing an undifferentiated avian cell culture using avian primordial germ cells Download PDF

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Publication number
CA2381666C
CA2381666C CA2381666A CA2381666A CA2381666C CA 2381666 C CA2381666 C CA 2381666C CA 2381666 A CA2381666 A CA 2381666A CA 2381666 A CA2381666 A CA 2381666A CA 2381666 C CA2381666 C CA 2381666C
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Canada
Prior art keywords
cells
avian
fibroblast
feeder
preconditioned
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CA2381666A
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English (en)
French (fr)
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CA2381666A1 (en
Inventor
James N. Pettite
Yi Guo Zhang
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North Carolina State University
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North Carolina State University
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Publication of CA2381666A1 publication Critical patent/CA2381666A1/en
Application granted granted Critical
Publication of CA2381666C publication Critical patent/CA2381666C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/13Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/04Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from germ cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2381666A 1999-08-09 2000-08-07 Method of producing an undifferentiated avian cell culture using avian primordial germ cells Expired - Fee Related CA2381666C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/370,708 1999-08-09
US09/370,708 US6333192B1 (en) 1999-08-09 1999-08-09 Method of producing an undifferentiated avian cell culture using avian primordial germ cells
PCT/US2000/021545 WO2001011019A1 (en) 1999-08-09 2000-08-07 Method of producing an undifferentiated avian cell culture using avian primordial germ cells

Publications (2)

Publication Number Publication Date
CA2381666A1 CA2381666A1 (en) 2001-02-15
CA2381666C true CA2381666C (en) 2012-02-21

Family

ID=23460834

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2381666A Expired - Fee Related CA2381666C (en) 1999-08-09 2000-08-07 Method of producing an undifferentiated avian cell culture using avian primordial germ cells

Country Status (12)

Country Link
US (2) US6333192B1 (enExample)
EP (2) EP1200556B1 (enExample)
JP (1) JP4841777B2 (enExample)
KR (1) KR100511827B1 (enExample)
AT (2) ATE355361T1 (enExample)
AU (1) AU782640B2 (enExample)
CA (1) CA2381666C (enExample)
DE (2) DE60033663T2 (enExample)
DK (1) DK1200556T3 (enExample)
ES (1) ES2282129T3 (enExample)
NZ (2) NZ516958A (enExample)
WO (1) WO2001011019A1 (enExample)

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AU3597093A (en) * 1992-01-27 1993-09-01 Embrex Inc. Gene transfer in poultry by introduction of embryo cells (in ovo)
US6333192B1 (en) * 1999-08-09 2001-12-25 North Carolina State University Method of producing an undifferentiated avian cell culture using avian primordial germ cells
IL136232A0 (en) * 2000-05-18 2001-05-20 Bar Ilan University Res Author Measurements of enzymatic activity in a single, individual cell in population
US20020162134A1 (en) * 2001-02-16 2002-10-31 Alexander Baguisi Primordial germ cell-based germ line production of birds
JP4381139B2 (ja) * 2001-08-13 2009-12-09 エンブレクス,インコーポレイテッド 鳥類の卵の処理方法
US7145057B2 (en) * 2002-02-01 2006-12-05 Origen Therapeutics, Inc. Chimeric bird from embryonic stem cells
WO2004065558A2 (en) * 2003-01-16 2004-08-05 North Carolina State University Depletion of endogenous primordial germ cells in avian species
IL154677A0 (en) 2003-02-27 2003-09-17 Univ Bar Ilan A method and apparatus for manipulating an individual cell
US7888110B2 (en) 2003-06-26 2011-02-15 Seng Enterprises Ltd. Pico liter well holding device and method of making the same
US9200245B2 (en) 2003-06-26 2015-12-01 Seng Enterprises Ltd. Multiwell plate
US8597597B2 (en) 2003-06-26 2013-12-03 Seng Enterprises Ltd. Picoliter well holding device and method of making the same
US7982751B2 (en) * 2003-07-11 2011-07-19 The University Of North Carolina Methods and systems for controlling a computer using a video image and for combining the video image with a computer desktop
KR100569168B1 (ko) * 2003-08-08 2006-04-07 (주)아비코아생명공학연구소 조류 정원줄기세포의 배양방법 및 이에 의해 수득한 조류정원줄기세포
US20050064524A1 (en) * 2003-08-11 2005-03-24 Mordechai Deutsch Population of cells utilizable for substance detection and methods and devices using same
BRPI0506610A (pt) * 2004-02-03 2007-05-02 Chemagis Ltd montelucaste sódico amorfo, processo de preparação de montelucaste sódico amorfo, composição farmacêutica, método de tratamento utilizando a administração de montelucaste sódico amorfo, co-precipitado amorfo de montelucaste sódico e processo de preparação de co-precipitado amorfo de montelucaste sódico
US7403647B2 (en) * 2004-09-13 2008-07-22 Seng Enterprises Ltd. Method for identifying an image of a well in an image of a well-bearing component
US20060110824A1 (en) * 2004-11-24 2006-05-25 Lih-Ren Chen Method for culturing avian primordial germ cells (PGCs) for a long period and preparing a medium for culturing avian PGCs for a long period
ES2352344T3 (es) 2005-01-25 2011-02-17 Seng Enterprises Limited Dispositivo de microfluido para estudio de células.
US20060174362A1 (en) 2005-02-01 2006-08-03 Origen Therapeutics, Inc. Long-term culture of avian primordial germ cells (PGCs)
DE102005034043B4 (de) * 2005-07-18 2019-12-12 Südzucker Aktiengesellschaft Mannheim/Ochsenfurt Gemisch, enthaltend L-Carnitin und Trehalulose, sowie Produkt enthaltend das Gemisch
US8288120B2 (en) 2005-11-03 2012-10-16 Seng Enterprises Ltd. Method for studying floating, living cells
US20060223999A1 (en) * 2006-05-10 2006-10-05 Chemagis Ltd. Process for preparing montelukast and precursors thereof
US9145540B1 (en) 2007-11-15 2015-09-29 Seng Enterprises Ltd. Device for the study of living cells
WO2009081409A2 (en) 2007-12-26 2009-07-02 Seng Enterprises Ltd. Device for the study of living cells
EP2464220A4 (en) 2009-08-13 2014-05-07 Crystal Bioscience Inc Transgenic animal for the production of antibodies with minimal complementarity-determining regions
US8865462B2 (en) * 2010-01-20 2014-10-21 Crystal Bioscience Inc. Sustained culture of avian gonocytes
US9150637B2 (en) 2010-11-05 2015-10-06 Baxalta Inc. Variant of antihemophilic factor VIII having increased specific activity
CN106106336A (zh) * 2016-06-23 2016-11-16 田东县浙缘农业科技有限公司 一种防治小鹅瘟的养鹅方法
CN106212384A (zh) * 2016-09-30 2016-12-14 界首市英琪养殖专业合作社 一种0~21日龄雏鹅的养殖方法
CN106508800A (zh) * 2016-10-28 2017-03-22 六安市裕安区固镇军明白鹅养殖专业合作社 一种皖西大白鹅放养繁育方法
CN106614283A (zh) * 2016-10-28 2017-05-10 六安市裕安区固镇军明白鹅养殖专业合作社 一种基于提高皖西大白鹅种鹅生产性能的饲养方法
AU2020344163A1 (en) * 2019-09-10 2022-03-31 Vital Meat Avian stem cells for the production of a food product
CN111500531A (zh) * 2020-05-15 2020-08-07 扬州大学 一种鸡PGCs在体外长期培养的方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU623922B2 (en) * 1988-08-04 1992-05-28 Amrad Operations Pty. Limited In vitro propagation of embryonic stem cells
US5340740A (en) 1992-05-15 1994-08-23 North Carolina State University Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process
US5690926A (en) 1992-10-08 1997-11-25 Vanderbilt University Pluripotential embryonic cells and methods of making same
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
WO1996006160A1 (en) * 1994-08-24 1996-02-29 Meiji Milk Products Co., Ltd. Method of culturing avian cells and cell line obtained thereby
FR2726003B1 (fr) 1994-10-21 2002-10-18 Agronomique Inst Nat Rech Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires
US5985659A (en) * 1996-05-21 1999-11-16 The Institute Of Physical And Chemical Research Embryonic stem cell lines obtained from C3H/HeN and DBA/1J mouse strains
ES2255174T3 (es) 1997-08-04 2006-06-16 Univ. Massachusetts, A Public Inst. Higher Educ. Of Commonwealth Massach. Repr By Its Amherst Campus Produccion de lineas de celulas germinales embrionarias (eg) aviares por cultivo prolongado de pgc, y uso de las mismas para clonacion y quimerizacion.
JP2003532364A (ja) 1997-08-04 2003-11-05 ユニバシティ オブ マサチューセッツ、ア パブリック インスチチユーション オブ ハイアー エデュケイション オブ ザ コモンウエルス オブ マサチューセッツ、アズ リプリゼンテッド バイ イッツ アマースト キャンパス トリ始原生殖細胞(pgc)細胞系とその長期間培養法
US6156569A (en) * 1997-08-04 2000-12-05 University Of Massachusetts Office Of Vice Chancellor For Research At Amherst Prolonged culturing of avian primordial germ cells (PGCs) using specific growth factors, use thereof to produce chimeric avians
CA2359775C (en) * 1999-02-11 2007-09-18 Jae Yong Han Avian pluripotent embryonic germ cell line
US6333192B1 (en) * 1999-08-09 2001-12-25 North Carolina State University Method of producing an undifferentiated avian cell culture using avian primordial germ cells
US6354242B1 (en) * 2000-03-23 2002-03-12 North Carolina State University Methods for gamete production in birds

Also Published As

Publication number Publication date
US20010021528A1 (en) 2001-09-13
DK1200556T3 (da) 2007-06-18
DE60043554D1 (de) 2010-01-28
DE60033663D1 (de) 2007-04-12
ES2282129T3 (es) 2007-10-16
US6333192B1 (en) 2001-12-25
EP1200556A1 (en) 2002-05-02
EP1200556A4 (en) 2003-07-09
AU782640B2 (en) 2005-08-18
KR20020029096A (ko) 2002-04-17
ATE355361T1 (de) 2006-03-15
NZ520338A (en) 2003-06-30
NZ516958A (en) 2002-08-28
JP2003506076A (ja) 2003-02-18
EP1200556B1 (en) 2007-02-28
DE60033663T2 (de) 2007-11-22
WO2001011019A1 (en) 2001-02-15
CA2381666A1 (en) 2001-02-15
ATE452182T1 (de) 2010-01-15
JP4841777B2 (ja) 2011-12-21
EP1780263B1 (en) 2009-12-16
US7422897B2 (en) 2008-09-09
KR100511827B1 (ko) 2005-09-02
EP1780263A2 (en) 2007-05-02
AU6526900A (en) 2001-03-05
EP1780263A3 (en) 2007-06-20

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