AU782640B2 - Method of producing an undifferentiated avian cell culture using avian primordial germ cells - Google Patents
Method of producing an undifferentiated avian cell culture using avian primordial germ cells Download PDFInfo
- Publication number
- AU782640B2 AU782640B2 AU65269/00A AU6526900A AU782640B2 AU 782640 B2 AU782640 B2 AU 782640B2 AU 65269/00 A AU65269/00 A AU 65269/00A AU 6526900 A AU6526900 A AU 6526900A AU 782640 B2 AU782640 B2 AU 782640B2
- Authority
- AU
- Australia
- Prior art keywords
- cells
- avian
- fibroblast
- feeder
- gonadal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 241000271566 Aves Species 0.000 title claims abstract description 137
- 238000000034 method Methods 0.000 title claims abstract description 71
- 210000004602 germ cell Anatomy 0.000 title claims abstract description 27
- 238000004113 cell culture Methods 0.000 title claims abstract description 15
- 210000004027 cell Anatomy 0.000 claims abstract description 225
- 230000002710 gonadal effect Effects 0.000 claims abstract description 81
- 210000001671 embryonic stem cell Anatomy 0.000 claims abstract description 52
- 239000011159 matrix material Substances 0.000 claims abstract description 51
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 47
- 238000000151 deposition Methods 0.000 claims abstract description 9
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 6
- 210000001811 primitive streak Anatomy 0.000 claims abstract description 6
- 210000002950 fibroblast Anatomy 0.000 claims description 54
- 230000002459 sustained effect Effects 0.000 claims description 27
- 241000287828 Gallus gallus Species 0.000 claims description 15
- 239000003636 conditioned culture medium Substances 0.000 claims description 14
- 241000286209 Phasianidae Species 0.000 claims description 10
- 210000002536 stromal cell Anatomy 0.000 claims description 9
- 210000004392 genitalia Anatomy 0.000 claims description 8
- 108090000581 Leukemia inhibitory factor Proteins 0.000 claims description 7
- 102000018233 Fibroblast Growth Factor Human genes 0.000 claims description 6
- 108050007372 Fibroblast Growth Factor Proteins 0.000 claims description 6
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 claims description 6
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims description 6
- 102100020880 Kit ligand Human genes 0.000 claims description 6
- 241000894007 species Species 0.000 claims description 6
- 210000000130 stem cell Anatomy 0.000 claims description 6
- 241000272525 Anas platyrhynchos Species 0.000 claims description 5
- 241000272814 Anser sp. Species 0.000 claims description 5
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 claims description 5
- 102000014429 Insulin-like growth factor Human genes 0.000 claims description 5
- 229940126864 fibroblast growth factor Drugs 0.000 claims description 5
- 239000003102 growth factor Substances 0.000 claims description 5
- 230000012010 growth Effects 0.000 claims description 4
- 230000001131 transforming effect Effects 0.000 claims description 4
- 102000004058 Leukemia inhibitory factor Human genes 0.000 claims description 3
- 108010039445 Stem Cell Factor Proteins 0.000 claims description 3
- 230000000153 supplemental effect Effects 0.000 claims 1
- 229960001727 tretinoin Drugs 0.000 claims 1
- 230000001143 conditioned effect Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 description 20
- LAQPKDLYOBZWBT-NYLDSJSYSA-N (2s,4s,5r,6r)-5-acetamido-2-{[(2s,3r,4s,5s,6r)-2-{[(2r,3r,4r,5r)-5-acetamido-1,2-dihydroxy-6-oxo-4-{[(2s,3s,4r,5s,6s)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxy}hexan-3-yl]oxy}-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy}-4-hydroxy-6-[(1r,2r)-1,2,3-trihydrox Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@H]([C@@H](NC(C)=O)C=O)[C@@H]([C@H](O)CO)O[C@H]1[C@H](O)[C@@H](O[C@]2(O[C@H]([C@H](NC(C)=O)[C@@H](O)C2)[C@H](O)[C@H](O)CO)C(O)=O)[C@@H](O)[C@@H](CO)O1 LAQPKDLYOBZWBT-NYLDSJSYSA-N 0.000 description 18
- 239000010410 layer Substances 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 11
- 102000004169 proteins and genes Human genes 0.000 description 11
- 238000011161 development Methods 0.000 description 9
- 230000018109 developmental process Effects 0.000 description 9
- 210000002257 embryonic structure Anatomy 0.000 description 9
- 210000002149 gonad Anatomy 0.000 description 9
- 238000010899 nucleation Methods 0.000 description 9
- 230000009261 transgenic effect Effects 0.000 description 9
- 239000013598 vector Substances 0.000 description 9
- 108020004414 DNA Proteins 0.000 description 8
- 235000013601 eggs Nutrition 0.000 description 8
- 238000002347 injection Methods 0.000 description 8
- 239000007924 injection Substances 0.000 description 8
- 230000004083 survival effect Effects 0.000 description 8
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 7
- 108091028043 Nucleic acid sequence Proteins 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 230000035755 proliferation Effects 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- 239000012091 fetal bovine serum Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 244000144977 poultry Species 0.000 description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
- 238000012258 culturing Methods 0.000 description 5
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 5
- 239000002953 phosphate buffered saline Substances 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 239000002356 single layer Substances 0.000 description 5
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 4
- 102100032352 Leukemia inhibitory factor Human genes 0.000 description 4
- 210000002304 esc Anatomy 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 238000004520 electroporation Methods 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 101710145634 Antigen 1 Proteins 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241001529936 Murinae Species 0.000 description 2
- NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000013020 embryo development Effects 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000006801 homologous recombination Effects 0.000 description 2
- 238000002744 homologous recombination Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- XNSAINXGIQZQOO-SRVKXCTJSA-N protirelin Chemical compound NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H]1NC(=O)CC1)CC1=CN=CN1 XNSAINXGIQZQOO-SRVKXCTJSA-N 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000003566 sealing material Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 208000003495 Coccidiosis Diseases 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 206010023076 Isosporiasis Diseases 0.000 description 1
- 101710177504 Kit ligand Proteins 0.000 description 1
- 101100407657 Mus musculus Pgc gene Proteins 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000700157 Rattus norvegicus Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102100038803 Somatotropin Human genes 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000001668 ameliorated effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000002459 blastocyst Anatomy 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000003711 chorioallantoic membrane Anatomy 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 230000008143 early embryonic development Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- -1 electroporation Substances 0.000 description 1
- 229940071106 ethylenediaminetetraacetate Drugs 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 210000002219 extraembryonic membrane Anatomy 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 229960004857 mitomycin Drugs 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 230000006461 physiological response Effects 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 210000001325 yolk sac Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/04—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from germ cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Gynecology & Obstetrics (AREA)
- Biotechnology (AREA)
- Reproductive Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Developmental Biology & Embryology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/370,708 US6333192B1 (en) | 1999-08-09 | 1999-08-09 | Method of producing an undifferentiated avian cell culture using avian primordial germ cells |
| US09/370708 | 1999-08-09 | ||
| PCT/US2000/021545 WO2001011019A1 (en) | 1999-08-09 | 2000-08-07 | Method of producing an undifferentiated avian cell culture using avian primordial germ cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU6526900A AU6526900A (en) | 2001-03-05 |
| AU782640B2 true AU782640B2 (en) | 2005-08-18 |
Family
ID=23460834
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU65269/00A Ceased AU782640B2 (en) | 1999-08-09 | 2000-08-07 | Method of producing an undifferentiated avian cell culture using avian primordial germ cells |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US6333192B1 (enExample) |
| EP (2) | EP1200556B1 (enExample) |
| JP (1) | JP4841777B2 (enExample) |
| KR (1) | KR100511827B1 (enExample) |
| AT (2) | ATE355361T1 (enExample) |
| AU (1) | AU782640B2 (enExample) |
| CA (1) | CA2381666C (enExample) |
| DE (2) | DE60033663T2 (enExample) |
| DK (1) | DK1200556T3 (enExample) |
| ES (1) | ES2282129T3 (enExample) |
| NZ (2) | NZ516958A (enExample) |
| WO (1) | WO2001011019A1 (enExample) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU3597093A (en) * | 1992-01-27 | 1993-09-01 | Embrex Inc. | Gene transfer in poultry by introduction of embryo cells (in ovo) |
| US6333192B1 (en) * | 1999-08-09 | 2001-12-25 | North Carolina State University | Method of producing an undifferentiated avian cell culture using avian primordial germ cells |
| IL136232A0 (en) * | 2000-05-18 | 2001-05-20 | Bar Ilan University Res Author | Measurements of enzymatic activity in a single, individual cell in population |
| US20020162134A1 (en) * | 2001-02-16 | 2002-10-31 | Alexander Baguisi | Primordial germ cell-based germ line production of birds |
| JP4381139B2 (ja) * | 2001-08-13 | 2009-12-09 | エンブレクス，インコーポレイテッド | 鳥類の卵の処理方法 |
| US7145057B2 (en) * | 2002-02-01 | 2006-12-05 | Origen Therapeutics, Inc. | Chimeric bird from embryonic stem cells |
| WO2004065558A2 (en) * | 2003-01-16 | 2004-08-05 | North Carolina State University | Depletion of endogenous primordial germ cells in avian species |
| IL154677A0 (en) | 2003-02-27 | 2003-09-17 | Univ Bar Ilan | A method and apparatus for manipulating an individual cell |
| US7888110B2 (en) | 2003-06-26 | 2011-02-15 | Seng Enterprises Ltd. | Pico liter well holding device and method of making the same |
| US9200245B2 (en) | 2003-06-26 | 2015-12-01 | Seng Enterprises Ltd. | Multiwell plate |
| US8597597B2 (en) | 2003-06-26 | 2013-12-03 | Seng Enterprises Ltd. | Picoliter well holding device and method of making the same |
| US7982751B2 (en) * | 2003-07-11 | 2011-07-19 | The University Of North Carolina | Methods and systems for controlling a computer using a video image and for combining the video image with a computer desktop |
| KR100569168B1 (ko) * | 2003-08-08 | 2006-04-07 | (주)아비코아생명공학연구소 | 조류 정원줄기세포의 배양방법 및 이에 의해 수득한 조류정원줄기세포 |
| US20050064524A1 (en) * | 2003-08-11 | 2005-03-24 | Mordechai Deutsch | Population of cells utilizable for substance detection and methods and devices using same |
| BRPI0506610A (pt) * | 2004-02-03 | 2007-05-02 | Chemagis Ltd | montelucaste sódico amorfo, processo de preparação de montelucaste sódico amorfo, composição farmacêutica, método de tratamento utilizando a administração de montelucaste sódico amorfo, co-precipitado amorfo de montelucaste sódico e processo de preparação de co-precipitado amorfo de montelucaste sódico |
| US7403647B2 (en) * | 2004-09-13 | 2008-07-22 | Seng Enterprises Ltd. | Method for identifying an image of a well in an image of a well-bearing component |
| US20060110824A1 (en) * | 2004-11-24 | 2006-05-25 | Lih-Ren Chen | Method for culturing avian primordial germ cells (PGCs) for a long period and preparing a medium for culturing avian PGCs for a long period |
| ES2352344T3 (es) | 2005-01-25 | 2011-02-17 | Seng Enterprises Limited | Dispositivo de microfluido para estudio de células. |
| US20060174362A1 (en) | 2005-02-01 | 2006-08-03 | Origen Therapeutics, Inc. | Long-term culture of avian primordial germ cells (PGCs) |
| DE102005034043B4 (de) * | 2005-07-18 | 2019-12-12 | Südzucker Aktiengesellschaft Mannheim/Ochsenfurt | Gemisch, enthaltend L-Carnitin und Trehalulose, sowie Produkt enthaltend das Gemisch |
| US8288120B2 (en) | 2005-11-03 | 2012-10-16 | Seng Enterprises Ltd. | Method for studying floating, living cells |
| US20060223999A1 (en) * | 2006-05-10 | 2006-10-05 | Chemagis Ltd. | Process for preparing montelukast and precursors thereof |
| US9145540B1 (en) | 2007-11-15 | 2015-09-29 | Seng Enterprises Ltd. | Device for the study of living cells |
| WO2009081409A2 (en) | 2007-12-26 | 2009-07-02 | Seng Enterprises Ltd. | Device for the study of living cells |
| EP2464220A4 (en) | 2009-08-13 | 2014-05-07 | Crystal Bioscience Inc | Transgenic animal for the production of antibodies with minimal complementarity-determining regions |
| US8865462B2 (en) * | 2010-01-20 | 2014-10-21 | Crystal Bioscience Inc. | Sustained culture of avian gonocytes |
| US9150637B2 (en) | 2010-11-05 | 2015-10-06 | Baxalta Inc. | Variant of antihemophilic factor VIII having increased specific activity |
| CN106106336A (zh) * | 2016-06-23 | 2016-11-16 | 田东县浙缘农业科技有限公司 | 一种防治小鹅瘟的养鹅方法 |
| CN106212384A (zh) * | 2016-09-30 | 2016-12-14 | 界首市英琪养殖专业合作社 | 一种0~21日龄雏鹅的养殖方法 |
| CN106508800A (zh) * | 2016-10-28 | 2017-03-22 | 六安市裕安区固镇军明白鹅养殖专业合作社 | 一种皖西大白鹅放养繁育方法 |
| CN106614283A (zh) * | 2016-10-28 | 2017-05-10 | 六安市裕安区固镇军明白鹅养殖专业合作社 | 一种基于提高皖西大白鹅种鹅生产性能的饲养方法 |
| AU2020344163A1 (en) * | 2019-09-10 | 2022-03-31 | Vital Meat | Avian stem cells for the production of a food product |
| CN111500531A (zh) * | 2020-05-15 | 2020-08-07 | 扬州大学 | 一种鸡PGCs在体外长期培养的方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU623922B2 (en) * | 1988-08-04 | 1992-05-28 | Amrad Operations Pty. Limited | In vitro propagation of embryonic stem cells |
| US5340740A (en) | 1992-05-15 | 1994-08-23 | North Carolina State University | Method of producing an avian embryonic stem cell culture and the avian embryonic stem cell culture produced by the process |
| US5690926A (en) | 1992-10-08 | 1997-11-25 | Vanderbilt University | Pluripotential embryonic cells and methods of making same |
| US5453357A (en) | 1992-10-08 | 1995-09-26 | Vanderbilt University | Pluripotential embryonic stem cells and methods of making same |
| WO1996006160A1 (en) * | 1994-08-24 | 1996-02-29 | Meiji Milk Products Co., Ltd. | Method of culturing avian cells and cell line obtained thereby |
| FR2726003B1 (fr) | 1994-10-21 | 2002-10-18 | Agronomique Inst Nat Rech | Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires |
| US5985659A (en) * | 1996-05-21 | 1999-11-16 | The Institute Of Physical And Chemical Research | Embryonic stem cell lines obtained from C3H/HeN and DBA/1J mouse strains |
| ES2255174T3 (es) | 1997-08-04 | 2006-06-16 | Univ. Massachusetts, A Public Inst. Higher Educ. Of Commonwealth Massach. Repr By Its Amherst Campus | Produccion de lineas de celulas germinales embrionarias (eg) aviares por cultivo prolongado de pgc, y uso de las mismas para clonacion y quimerizacion. |
| JP2003532364A (ja) | 1997-08-04 | 2003-11-05 | ユニバシティ オブ マサチューセッツ、ア パブリック インスチチユーション オブ ハイアー エデュケイション オブ ザ コモンウエルス オブ マサチューセッツ、アズ リプリゼンテッド バイ イッツ アマースト キャンパス | トリ始原生殖細胞（ｐｇｃ）細胞系とその長期間培養法 |
| US6156569A (en) * | 1997-08-04 | 2000-12-05 | University Of Massachusetts Office Of Vice Chancellor For Research At Amherst | Prolonged culturing of avian primordial germ cells (PGCs) using specific growth factors, use thereof to produce chimeric avians |
| CA2359775C (en) * | 1999-02-11 | 2007-09-18 | Jae Yong Han | Avian pluripotent embryonic germ cell line |
| US6333192B1 (en) * | 1999-08-09 | 2001-12-25 | North Carolina State University | Method of producing an undifferentiated avian cell culture using avian primordial germ cells |
| US6354242B1 (en) * | 2000-03-23 | 2002-03-12 | North Carolina State University | Methods for gamete production in birds |
-
1999
- 1999-08-09 US US09/370,708 patent/US6333192B1/en not_active Expired - Lifetime
-
2000
- 2000-08-07 NZ NZ516958A patent/NZ516958A/xx not_active IP Right Cessation
- 2000-08-07 WO PCT/US2000/021545 patent/WO2001011019A1/en not_active Ceased
- 2000-08-07 AU AU65269/00A patent/AU782640B2/en not_active Ceased
- 2000-08-07 EP EP00952602A patent/EP1200556B1/en not_active Expired - Lifetime
- 2000-08-07 JP JP2001515806A patent/JP4841777B2/ja not_active Expired - Fee Related
- 2000-08-07 AT AT00952602T patent/ATE355361T1/de active
- 2000-08-07 ES ES00952602T patent/ES2282129T3/es not_active Expired - Lifetime
- 2000-08-07 AT AT06127294T patent/ATE452182T1/de not_active IP Right Cessation
- 2000-08-07 EP EP06127294A patent/EP1780263B1/en not_active Expired - Lifetime
- 2000-08-07 CA CA2381666A patent/CA2381666C/en not_active Expired - Fee Related
- 2000-08-07 DE DE60033663T patent/DE60033663T2/de not_active Expired - Lifetime
- 2000-08-07 DK DK00952602T patent/DK1200556T3/da active
- 2000-08-07 DE DE60043554T patent/DE60043554D1/de not_active Expired - Lifetime
- 2000-08-07 KR KR10-2002-7001745A patent/KR100511827B1/ko not_active Expired - Fee Related
-
2001
- 2001-01-08 US US09/757,054 patent/US7422897B2/en not_active Expired - Fee Related
-
2002
- 2002-07-23 NZ NZ520338A patent/NZ520338A/xx not_active IP Right Cessation
Non-Patent Citations (1)
| Title |
|---|
| ALLIOLI ET AL. (1994), DEV. BIOL., 165, 30-37 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US20010021528A1 (en) | 2001-09-13 |
| DK1200556T3 (da) | 2007-06-18 |
| DE60043554D1 (de) | 2010-01-28 |
| DE60033663D1 (de) | 2007-04-12 |
| ES2282129T3 (es) | 2007-10-16 |
| US6333192B1 (en) | 2001-12-25 |
| EP1200556A1 (en) | 2002-05-02 |
| EP1200556A4 (en) | 2003-07-09 |
| KR20020029096A (ko) | 2002-04-17 |
| ATE355361T1 (de) | 2006-03-15 |
| NZ520338A (en) | 2003-06-30 |
| NZ516958A (en) | 2002-08-28 |
| CA2381666C (en) | 2012-02-21 |
| JP2003506076A (ja) | 2003-02-18 |
| EP1200556B1 (en) | 2007-02-28 |
| DE60033663T2 (de) | 2007-11-22 |
| WO2001011019A1 (en) | 2001-02-15 |
| CA2381666A1 (en) | 2001-02-15 |
| ATE452182T1 (de) | 2010-01-15 |
| JP4841777B2 (ja) | 2011-12-21 |
| EP1780263B1 (en) | 2009-12-16 |
| US7422897B2 (en) | 2008-09-09 |
| KR100511827B1 (ko) | 2005-09-02 |
| EP1780263A2 (en) | 2007-05-02 |
| AU6526900A (en) | 2001-03-05 |
| EP1780263A3 (en) | 2007-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU782640B2 (en) | Method of producing an undifferentiated avian cell culture using avian primordial germ cells | |
| US5656479A (en) | Avian embryonic stem cells | |
| US6500668B2 (en) | Culture medium for avian embryonic cells | |
| US6156569A (en) | Prolonged culturing of avian primordial germ cells (PGCs) using specific growth factors, use thereof to produce chimeric avians | |
| US8962311B2 (en) | Method of obtaining chicken embryonic stem cells | |
| AU736087B2 (en) | Avian primordial germ cell (PGC) cell line and a method for long term culturing thereof | |
| JP4376901B2 (ja) | 精原細胞を利用した鳥類キメラの生産方法及び鳥類キメラ | |
| WO2000008132A1 (en) | Prolonged culturing of avian primordial germ cells using specific growth factors and use thereof | |
| MXPA00001301A (en) | Avian primordial germ cell (pgc) cell line and a method for long term culturing thereof |