CA2378070A1 - Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede - Google Patents
Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede Download PDFInfo
- Publication number
- CA2378070A1 CA2378070A1 CA002378070A CA2378070A CA2378070A1 CA 2378070 A1 CA2378070 A1 CA 2378070A1 CA 002378070 A CA002378070 A CA 002378070A CA 2378070 A CA2378070 A CA 2378070A CA 2378070 A1 CA2378070 A1 CA 2378070A1
- Authority
- CA
- Canada
- Prior art keywords
- sequence
- primer
- heel
- heeled
- population
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/131—Modifications characterised by incorporating a restriction site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La présente invention concerne des procédé ainsi que des amorces d'acide nucléique et des kits les renfermant qui permettent de réaliser une amplification efficace de séquences d'acides nucléiques prises dans un échantillon, plus particulièrement de séquences d'acide nucléiques dont la représentation au sein dudit échantillon est relativement faible au départ.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14466699P | 1999-07-19 | 1999-07-19 | |
US60/144,666 | 1999-07-19 | ||
PCT/EP2000/006887 WO2001006004A2 (fr) | 1999-07-19 | 2000-07-19 | Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2378070A1 true CA2378070A1 (fr) | 2001-01-25 |
Family
ID=22509592
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002378070A Abandoned CA2378070A1 (fr) | 1999-07-19 | 2000-07-19 | Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1196639A2 (fr) |
JP (1) | JP2003505034A (fr) |
KR (1) | KR20020034161A (fr) |
AU (1) | AU6695600A (fr) |
CA (1) | CA2378070A1 (fr) |
WO (1) | WO2001006004A2 (fr) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1275738A1 (fr) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Procédé pour la synthèse aléatoire et l'amplification d'ADNc |
US7414111B2 (en) | 2001-09-19 | 2008-08-19 | Alexion Pharmaceuticals, Inc. | Engineered templates and their use in single primer amplification |
DE60234369D1 (de) * | 2001-09-19 | 2009-12-24 | Alexion Pharma Inc | Manipulierte matrizen und ihre verwendung bei der single-primer-amplifikation |
GB0216026D0 (en) * | 2002-07-10 | 2002-08-21 | Cambridge Biotechnology Ltd | Nucleic acid amplification method |
US20040219565A1 (en) | 2002-10-21 | 2004-11-04 | Sakari Kauppinen | Oligonucleotides useful for detecting and analyzing nucleic acids of interest |
ES2380923T3 (es) * | 2004-04-07 | 2012-05-21 | Exiqon A/S | Procedimientos de cuantificación de microARN y ARN interferentes pequeños |
US8192937B2 (en) | 2004-04-07 | 2012-06-05 | Exiqon A/S | Methods for quantification of microRNAs and small interfering RNAs |
KR100590621B1 (ko) * | 2004-09-21 | 2006-06-19 | (주)지노믹트리 | 하이힐 프라이머를 이용한 rna의 선형 증폭방법 |
JP4777631B2 (ja) * | 2004-09-27 | 2011-09-21 | 株式会社日立ハイテクノロジーズ | 核酸増幅分析法および装置 |
ES2403729T3 (es) * | 2007-04-05 | 2013-05-21 | Genera Biosystems Limited | Composiciones y métodos de detección |
CN102685048B (zh) * | 2012-05-28 | 2014-10-29 | 上海师范大学 | 一种突发信号盲均衡算法 |
WO2016082057A1 (fr) * | 2014-11-25 | 2016-06-02 | 深圳华大基因研究院 | Procédé de construction d'une banque de séquençage d'adn libre |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU7653691A (en) * | 1990-04-05 | 1991-10-30 | United States of America, as represented by the Secretary, U.S. Department of Commerce, The | Modified rna template-specific polymerase chain reaction |
GB9618050D0 (en) * | 1996-08-29 | 1996-10-09 | Cancer Res Campaign Tech | Global amplification of nucleic acids |
GB9817055D0 (en) * | 1998-08-05 | 1998-09-30 | Medical Res Council | Reverse transcription and amplification processes and primers therefore |
-
2000
- 2000-07-19 EP EP00954533A patent/EP1196639A2/fr not_active Withdrawn
- 2000-07-19 JP JP2001511213A patent/JP2003505034A/ja active Pending
- 2000-07-19 AU AU66956/00A patent/AU6695600A/en not_active Abandoned
- 2000-07-19 CA CA002378070A patent/CA2378070A1/fr not_active Abandoned
- 2000-07-19 KR KR1020027000769A patent/KR20020034161A/ko not_active Application Discontinuation
- 2000-07-19 WO PCT/EP2000/006887 patent/WO2001006004A2/fr not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
KR20020034161A (ko) | 2002-05-08 |
EP1196639A2 (fr) | 2002-04-17 |
AU6695600A (en) | 2001-02-05 |
JP2003505034A (ja) | 2003-02-12 |
WO2001006004A2 (fr) | 2001-01-25 |
WO2001006004A3 (fr) | 2001-08-09 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Dead |