CA2378070A1 - Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede - Google Patents

Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede Download PDF

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Publication number
CA2378070A1
CA2378070A1 CA002378070A CA2378070A CA2378070A1 CA 2378070 A1 CA2378070 A1 CA 2378070A1 CA 002378070 A CA002378070 A CA 002378070A CA 2378070 A CA2378070 A CA 2378070A CA 2378070 A1 CA2378070 A1 CA 2378070A1
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CA
Canada
Prior art keywords
sequence
primer
heel
heeled
population
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002378070A
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English (en)
Inventor
Peter Richardson
Peter Cox
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cambridge University Technical Services Ltd CUTS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2378070A1 publication Critical patent/CA2378070A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des procédé ainsi que des amorces d'acide nucléique et des kits les renfermant qui permettent de réaliser une amplification efficace de séquences d'acides nucléiques prises dans un échantillon, plus particulièrement de séquences d'acide nucléiques dont la représentation au sein dudit échantillon est relativement faible au départ.
CA002378070A 1999-07-19 2000-07-19 Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede Abandoned CA2378070A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14466699P 1999-07-19 1999-07-19
US60/144,666 1999-07-19
PCT/EP2000/006887 WO2001006004A2 (fr) 1999-07-19 2000-07-19 Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede

Publications (1)

Publication Number Publication Date
CA2378070A1 true CA2378070A1 (fr) 2001-01-25

Family

ID=22509592

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002378070A Abandoned CA2378070A1 (fr) 1999-07-19 2000-07-19 Procede d'amplification de sequences d'acide nucleique peu abondantes et moyens mis en oeuvre pour ce procede

Country Status (6)

Country Link
EP (1) EP1196639A2 (fr)
JP (1) JP2003505034A (fr)
KR (1) KR20020034161A (fr)
AU (1) AU6695600A (fr)
CA (1) CA2378070A1 (fr)
WO (1) WO2001006004A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1275738A1 (fr) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Procédé pour la synthèse aléatoire et l'amplification d'ADNc
US7414111B2 (en) 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
DE60234369D1 (de) * 2001-09-19 2009-12-24 Alexion Pharma Inc Manipulierte matrizen und ihre verwendung bei der single-primer-amplifikation
GB0216026D0 (en) * 2002-07-10 2002-08-21 Cambridge Biotechnology Ltd Nucleic acid amplification method
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
ES2380923T3 (es) * 2004-04-07 2012-05-21 Exiqon A/S Procedimientos de cuantificación de microARN y ARN interferentes pequeños
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
KR100590621B1 (ko) * 2004-09-21 2006-06-19 (주)지노믹트리 하이힐 프라이머를 이용한 ｒｎａ의 선형 증폭방법
JP4777631B2 (ja) * 2004-09-27 2011-09-21 株式会社日立ハイテクノロジーズ 核酸増幅分析法および装置
ES2403729T3 (es) * 2007-04-05 2013-05-21 Genera Biosystems Limited Composiciones y métodos de detección
CN102685048B (zh) * 2012-05-28 2014-10-29 上海师范大学 一种突发信号盲均衡算法
WO2016082057A1 (fr) * 2014-11-25 2016-06-02 深圳华大基因研究院 Procédé de construction d'une banque de séquençage d'adn libre

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7653691A (en) * 1990-04-05 1991-10-30 United States of America, as represented by the Secretary, U.S. Department of Commerce, The Modified rna template-specific polymerase chain reaction
GB9618050D0 (en) * 1996-08-29 1996-10-09 Cancer Res Campaign Tech Global amplification of nucleic acids
GB9817055D0 (en) * 1998-08-05 1998-09-30 Medical Res Council Reverse transcription and amplification processes and primers therefore

Also Published As

Publication number Publication date
KR20020034161A (ko) 2002-05-08
EP1196639A2 (fr) 2002-04-17
AU6695600A (en) 2001-02-05
JP2003505034A (ja) 2003-02-12
WO2001006004A2 (fr) 2001-01-25
WO2001006004A3 (fr) 2001-08-09

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