CA2376532A1 - Biocapteurs utilisant des polymeres conjugues a charge neutre - Google Patents

Biocapteurs utilisant des polymeres conjugues a charge neutre Download PDF

Info

Publication number
CA2376532A1
CA2376532A1 CA002376532A CA2376532A CA2376532A1 CA 2376532 A1 CA2376532 A1 CA 2376532A1 CA 002376532 A CA002376532 A CA 002376532A CA 2376532 A CA2376532 A CA 2376532A CA 2376532 A1 CA2376532 A1 CA 2376532A1
Authority
CA
Canada
Prior art keywords
conjugated polymer
biomolecule
neutral conjugated
charge neutral
electrodes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002376532A
Other languages
English (en)
Inventor
Song Shi
Changming Li
Vi-En Choong
George Maracas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Motorola Solutions Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2376532A1 publication Critical patent/CA2376532A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00709—Type of synthesis
    • B01J2219/00713—Electrochemical synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds

Abstract

La présente invention concerne un polymère conjugué à charge neutre ou des copolymères qui possèdent un groupe fonctionnel permettant la liaison d'une sonde biomoléculaire. Cette invention concerne aussi des électrodes et un réseau d'électrodes en contact électrique avec ces polymères, et une sonde biomoléculaire est liée de façon covalente au polymère. Cette invention comprend des biocapteurs qui utilisent les électrodes revêtues du polymère conjugué, et la liaison de la sonde biomoléculaire est détectée par une composante électrique telle qu'une impédance relative au courant alternatif.
CA002376532A 1999-06-10 2000-06-09 Biocapteurs utilisant des polymeres conjugues a charge neutre Abandoned CA2376532A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13843799P 1999-06-10 1999-06-10
US60/138,437 1999-06-10
PCT/US2000/015832 WO2000077523A1 (fr) 1999-06-10 2000-06-09 Biocapteurs utilisant des polymeres conjugues a charge neutre

Publications (1)

Publication Number Publication Date
CA2376532A1 true CA2376532A1 (fr) 2000-12-21

Family

ID=22482012

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002376532A Abandoned CA2376532A1 (fr) 1999-06-10 2000-06-09 Biocapteurs utilisant des polymeres conjugues a charge neutre

Country Status (5)

Country Link
EP (1) EP1190253A1 (fr)
JP (1) JP2003508730A (fr)
AU (1) AU776997B2 (fr)
CA (1) CA2376532A1 (fr)
WO (1) WO2000077523A1 (fr)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19938138C2 (de) * 1999-08-16 2003-02-13 November Ag Molekulare Medizin Verfahren und Vorrichtung zur Identifikation einer Biopolymersequenz auf Festkörperoberflächen
US6824669B1 (en) 2000-02-17 2004-11-30 Motorola, Inc. Protein and peptide sensors using electrical detection methods
DE10022750A1 (de) * 2000-05-10 2001-11-22 Wolfgang Schuhmann Verfahren zur Immobilisierung von Erkennungskomponenten
US7063978B2 (en) 2001-11-01 2006-06-20 3M Innovative Properties Company Coated film laminate having an electrically conductive surface
DE10228260A1 (de) * 2002-06-25 2004-01-22 Bayer Ag Methode und Vorrichtung zum impedimetrischen Nachweis eines oder mehrerer Analyten in einer Probe
US7598344B2 (en) * 2002-09-04 2009-10-06 Board Of Regents, The University Of Texas System Composition, method and use of bi-functional biomaterials
AU2003285301A1 (en) * 2002-10-28 2004-05-13 Apibio Sas Biochip and method for processing a plurality of fiochips
AU2003266711A1 (en) * 2002-11-14 2004-06-03 Cosel Co., Ltd. Method of detecting hybridization
FR2849038B1 (fr) 2002-12-19 2005-03-11 Apibio Nouveaux pyrroles substitues avec des oligonucleotides, polymeres electroactifs et leurs utilisations
DE10311315A1 (de) * 2003-03-14 2004-09-30 Apibio Sas Verfahren und Vorrichtung zur Detektion von Biomolekülen
DE10319155B4 (de) * 2003-04-29 2008-02-14 Bruker Daltonik Gmbh Elektrisch auslesbare Bindungen von Analytmolekülen an immobilisierten Sondenmolekülen
CN1914331A (zh) 2004-02-06 2007-02-14 拜尔健康护理有限责任公司 作为生物传感器的内部参照的可氧化种类和使用方法
SG151335A1 (en) 2004-04-01 2009-04-30 Univ Nanyang Addressable chem/bio chip array
KR101477948B1 (ko) 2005-09-30 2014-12-30 바이엘 헬스케어 엘엘씨 게이트형 전압 전류 측정 분석 구간 결정 방법
KR100663713B1 (ko) * 2005-12-16 2007-01-03 성균관대학교산학협력단 신규한 폴리디아세틸렌 초분자체 색 전이 센서
BRPI0717620A2 (pt) 2006-10-24 2013-10-22 Bayer Healthcare Llc Amperometria de decaimento transitório
WO2009076302A1 (fr) 2007-12-10 2009-06-18 Bayer Healthcare Llc Marqueurs de contrôle pour la détection automatique d'une solution de contrôle et procédés d'utilisation
FR2962445B1 (fr) 2010-07-08 2013-06-28 Biomerieux Sa Procede de detection et d'identification directe d'un microorganisme dans un echantillon biologique dilue dans un bouillon d'enrichissement
CN102520187B (zh) * 2011-11-23 2014-06-18 江南大学 一种基于聚苯胺纳米金复合膜免疫传感器的制备方法及其应用
JP6185935B2 (ja) * 2012-01-27 2017-08-23 ユニバーシティ オブ テネシー リサーチ ファウンデーション 交流動電によるバイオマーカーの検出のための方法および装置

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2703359B1 (fr) * 1993-03-31 1995-06-23 Cis Bio Int Copolymère nucléotide(s)/polymère conducteur électronique ; son procédé de préparation et son utilisation .
FR2720832A1 (fr) * 1994-04-22 1995-12-08 Francis Garnier Electrodes et membranes électroactives à base de peptides bioactifs, pour la reconnaissance, l'extraction ou le relargage d'espèces biologiquement actives.
DE19507413A1 (de) * 1994-05-06 1995-11-09 Bayer Ag Leitfähige Beschichtungen
KR0162864B1 (ko) * 1995-01-19 1999-01-15 김은영 가용 전기전도성 폴리피롤의 제조방법

Also Published As

Publication number Publication date
AU5475700A (en) 2001-01-02
JP2003508730A (ja) 2003-03-04
EP1190253A1 (fr) 2002-03-27
AU776997B2 (en) 2004-09-30
WO2000077523A1 (fr) 2000-12-21

Similar Documents

Publication Publication Date Title
AU776997B2 (en) Biosensors which utilize charge neutral conjugated polymers
Wang et al. New label-free DNA recognition based on doping nucleic-acid probes within conducting polymer films
Xiao et al. Carbon paste electrode modified with duplex molecularly imprinted polymer hybrid film for metronidazole detection
Caruana et al. Enzyme-Amplified Amperometric Detection of Hybridization and of a Single Base Pair Mutation in an 18 Base Oligonucleotide on a 7 µm Diameter Microelectrode
Aoki et al. Electrochemical Detection of a One‐Base Mismatch in an Oligonucleotide Using Ion‐Channel Sensors with Self‐Assembled PNA Monolayers
Ghita et al. Dopamine voltammetry at overoxidised polyindole electrodes
AU2002321531B2 (en) Methods for producing highly sensitive potentiometric sensors
Li et al. Impedance labelless detection-based polypyrrole DNA biosensor
CA2393766A1 (fr) Matrice de biopuces, haute densite, adressable par colonnes et rangees
Aoki et al. Trace analysis of an oligonucleotide with a specific sequence using PNA-based ion-channel sensors
US6468785B1 (en) Doped conducting polymers applications and methods
Aydemir et al. New immobilisation method for oligonucleotides on electrodes enables highly-sensitive, electrochemical label-free gene sensing
Bizid et al. Direct Electrochemical DNA Sensor based on a new redox oligomer modified with ferrocene and carboxylic acid: Application to the detection of Mycobacterium tuberculosis mutant strain
Hashimoto et al. Preliminary evaluation of electrochemical PNA array for detection of single base mismatch mutations
CA2432864A1 (fr) Methode de detection par mesure de l'impedance d'une ou plusieurs substances a analyser dans un echantillon, et dispositif connexe
US7879594B2 (en) Method and device for detecting biomolecules
Porfir’eva et al. An electrochemical DNA sensor for doxorubicin based on a polyelectrolyte complex and aminated thiacalix [4] arene
Calvo-Muñoz et al. Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly
JP3760158B2 (ja) 新規の伝導性高分子、これを利用したセンサー及び標的物質検出方法
JP2006510366A (ja) ヌクレオチド配列のｐｃｒ増幅および検出方法および装置
Shamsi et al. Probing nucleobase mismatch variations by electrochemical techniques: exploring the effects of position and nature of the single-nucleotide mismatch
CA2401830A1 (fr) Quantification des molecules cibles contenues dans un liquide
CA2441180C (fr) Procede permettant la detection electrochimique de la complementarite d'une paire de nucleobase
CN115201302A (zh) 一种电化学传感器及其制备方法与应用
US20020051975A1 (en) Reporterless genosensors using electrical detection methods

Legal Events

Date Code Title Description
FZDE Dead