CA2375210A1 - Tests homogenes permettant de determiner les fractions de lipoproteine de maniere sequentielle - Google Patents

Tests homogenes permettant de determiner les fractions de lipoproteine de maniere sequentielle Download PDF

Info

Publication number
CA2375210A1
CA2375210A1 CA002375210A CA2375210A CA2375210A1 CA 2375210 A1 CA2375210 A1 CA 2375210A1 CA 002375210 A CA002375210 A CA 002375210A CA 2375210 A CA2375210 A CA 2375210A CA 2375210 A1 CA2375210 A1 CA 2375210A1
Authority
CA
Canada
Prior art keywords
cholesterol
complex
hdl
sample
lipoprotein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002375210A
Other languages
English (en)
Inventor
Alan T. Remaley
Maureen L. Sampson
Gyorgy Csako
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Health and Human Services
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2375210A1 publication Critical patent/CA2375210A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des nouvelles analyses homogènes permettant de déterminer la quantité de cholestérol à lipoprotéines de basse densité (LDL-C) et de cholestérol à lipoprotéines de haute densité (HDL-C), ainsi que la quantité totale de cholestérol dans un échantillon. Le procédé consiste : à former un complexe composé d'une première fraction de lipoprotéine et d'un agent complexant, tel qu'un anticorps, à l'aide d'enzymes afin de détecter le cholestérol dans la fraction de lipoprotéine non complexée ; à mesurer la quantité de cholestérol dans la fraction de lipoprotéine non complexée de manière à fournir une première valeur de cholestérol ; puis, à séparer la fraction de lipoprotéine complexée du complexant de sorte que le cholestérol soit disponible pour servir de substrat aux enzymes. La quantité totale de cholestérol présent dans l'échantillon peut ainsi être déterminée. En outre, la première valeur de cholestérol obtenue peut être soustraite de la quantité totale de cholestérol de manière à obtenir une valeur pour la première fraction de lipoprotéine présente dans l'échantillon. Eventuellement, une analyse de la triglycéride peut également être effectuée sur l'échantillon contenu dans le tube.
CA002375210A 1999-05-28 2000-05-26 Tests homogenes permettant de determiner les fractions de lipoproteine de maniere sequentielle Abandoned CA2375210A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13670999P 1999-05-28 1999-05-28
US60/136,709 1999-05-28
PCT/US2000/014827 WO2000073797A2 (fr) 1999-05-28 2000-05-26 Tests homogenes permettant de determiner les fractions de lipoproteine de maniere sequentielle

Publications (1)

Publication Number Publication Date
CA2375210A1 true CA2375210A1 (fr) 2000-12-07

Family

ID=22474024

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002375210A Abandoned CA2375210A1 (fr) 1999-05-28 2000-05-26 Tests homogenes permettant de determiner les fractions de lipoproteine de maniere sequentielle

Country Status (5)

Country Link
EP (1) EP1183535A2 (fr)
JP (1) JP2003501630A (fr)
AU (1) AU771951B2 (fr)
CA (1) CA2375210A1 (fr)
WO (1) WO2000073797A2 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7939283B2 (en) 2001-11-01 2011-05-10 Fisher Scientific Company L.L.C. Analyte binding turbidity assay
EP1577398A4 (fr) * 2002-12-13 2006-07-26 Denka Seiken Kk Methode de quantification multiple du cholesterol dans des lipoproteines a faible densite
US7625721B2 (en) 2004-02-03 2009-12-01 Polymer Technology Systems, Inc. Non-precipitating bodily fluid analysis system
US7435577B2 (en) 2004-02-03 2008-10-14 Polymer Technology Systems, Inc. Direct measurement of chlolesterol from low density lipoprotein with test strip
JP4647927B2 (ja) * 2004-03-31 2011-03-09 デンカ生研株式会社 低密度リポ蛋白中コレステロールのマルチ定量法
US20060062690A1 (en) 2004-08-17 2006-03-23 Polymer Technology Systems, Inc. Apparatus and method of manufacturing bodily fluid test strip
CA2576453C (fr) * 2004-08-17 2013-12-03 Polymer Technology Systems, Inc. Systeme d'analyse d'un fluide corporel ne precipitant pas
CN100365409C (zh) * 2005-06-01 2008-01-30 王贤理 一种测定低密度脂蛋白胆固醇的试剂及制备方法
JP5129572B2 (ja) * 2005-09-30 2013-01-30 デンカ生研株式会社 生体試料中の２項目の測定対象物の同時分別定量方法
GB0526051D0 (en) * 2005-12-21 2006-02-01 Oxford Biosensors Ltd Cholesterol sensor
CN101078729B (zh) * 2006-05-10 2012-07-18 富士胶片株式会社 高密度脂蛋白胆固醇的测定方法
CN105044364B (zh) * 2015-05-02 2016-09-14 王贤俊 一种采用联合遮蔽法制备的ldl-c检测试剂盒
WO2017007966A1 (fr) * 2015-07-07 2017-01-12 Ashmaig Mohmed E Méthodes de détermination d'un taux de phospholipides et de lipoprotéines de haute densité dans un échantillon
CN113631272B (zh) * 2019-03-19 2022-12-20 美国西门子医学诊断股份有限公司 减轻疏水分析物的体外诊断测定中的脂蛋白干扰的组合物、装置和方法
CN111893163B (zh) * 2020-08-06 2023-01-17 武汉生之源生物科技股份有限公司 一种高密度脂蛋白3胆固醇检测试剂盒、制备方法及用途
CN112114127A (zh) * 2020-09-09 2020-12-22 武汉生之源生物科技股份有限公司 一种甘胆酸均相酶免疫测定试剂盒及其制备方法和应用
CN112695071B (zh) * 2020-12-16 2022-12-30 浙江伊利康生物技术有限公司 一种高密度脂蛋白3的测定试剂、方法和试剂盒

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2165947T3 (es) * 1995-07-21 2002-04-01 Wako Pure Chem Ind Ltd Metodo para medir la cantidad de un constituyente contenido en una lipoproteina especifica.
US5814472A (en) * 1997-05-13 1998-09-29 Wako Pure Chemical Industries, Ltd. Measurement of LDL-cholesterol

Also Published As

Publication number Publication date
WO2000073797A3 (fr) 2001-09-13
AU5449300A (en) 2000-12-18
AU771951B2 (en) 2004-04-08
EP1183535A2 (fr) 2002-03-06
WO2000073797A9 (fr) 2002-02-21
WO2000073797A2 (fr) 2000-12-07
JP2003501630A (ja) 2003-01-14

Similar Documents

Publication Publication Date Title
AU771951B2 (en) Homogeneous tests for sequentially determining lipoprotein fractions
JP2600065B2 (ja) 高密度リポ蛋白中のコレステロールの定量法
US20160177371A1 (en) Method of pretreatment of sample for quantitating cholesterol and method for quantitating cholesterol in specific lipoproteins by using the same
US8785146B2 (en) Method for quantitative measurements of HDL-C and LDL-C
JPH09313200A (ja) Ｌｄｌコレステロールの定量方法
EP0878716B1 (fr) Mesure du cholestérol des lipoprotéines à faible densité (LDL)
US8765394B2 (en) Method of quantifying cholesterol in high density lipoprotein and reagent compositions
CA2589459C (fr) Procede de mesure du cholesterol dans des lipoproteines du type residuelles
JPH09285298A (ja) Ｈｄｌ−コレステロールの測定方法及び測定用試薬
JP4542234B2 (ja) コレステロール定量用試料の前処理方法およびこれを利用する特定のリポ蛋白中のコレステロール定量法
EP0676642A1 (fr) Méthode d'analyse quantitative d'un composant dans une fraction lipoprotéinique
AU2015202990B2 (en) Method of pretreatment of sample for quantitating cholesterol and method for quantitating cholesterol in specific lipoproteins by using the same
Winder et al. ACP Broad Sheet no 151: September 1997. Investigation of dyslipidaemias.
CA2117745A1 (fr) Traitement d'echantillons contenant des lipoproteines
AU2012203506A1 (en) Method of pretreatment of sample for quantitating cholesterol and method for quantitating cholesterol in specific lipoproteins by using the same
Soh et al. 17 LIPIDS AND DYSLIPOPROTEINEMIA
KR20000015925A (ko) 엘디엘 콜레스테롤의 정량방법

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued