CA2372485A1 - Cell concentration and lysate clearance using paramagnetic particles - Google Patents

Cell concentration and lysate clearance using paramagnetic particles Download PDF

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Publication number
CA2372485A1
CA2372485A1 CA002372485A CA2372485A CA2372485A1 CA 2372485 A1 CA2372485 A1 CA 2372485A1 CA 002372485 A CA002372485 A CA 002372485A CA 2372485 A CA2372485 A CA 2372485A CA 2372485 A1 CA2372485 A1 CA 2372485A1
Authority
CA
Canada
Prior art keywords
magnetic particles
particles
solution
magnetic
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002372485A
Other languages
English (en)
French (fr)
Inventor
Rex M. Bitner
Craig E. Smith
Jacqueline Sankbeil
Braeden L. Butler
Douglas H. White
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of CA2372485A1 publication Critical patent/CA2372485A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002372485A 1999-05-14 1999-12-30 Cell concentration and lysate clearance using paramagnetic particles Abandoned CA2372485A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13415699P 1999-05-14 1999-05-14
US60/134,156 1999-05-14
PCT/US1999/031207 WO2000070040A1 (en) 1999-05-14 1999-12-30 Cell concentration and lysate clearance using paramagnetic particles

Publications (1)

Publication Number Publication Date
CA2372485A1 true CA2372485A1 (en) 2000-11-23

Family

ID=22462019

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002372485A Abandoned CA2372485A1 (en) 1999-05-14 1999-12-30 Cell concentration and lysate clearance using paramagnetic particles

Country Status (6)

Country Link
EP (1) EP1179058B1 (https=)
JP (2) JP4551568B2 (https=)
AT (2) ATE521703T1 (https=)
AU (1) AU778486B2 (https=)
CA (1) CA2372485A1 (https=)
WO (1) WO2000070040A1 (https=)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
DE10033991A1 (de) * 2000-07-12 2002-01-24 Qiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren
ES2273915T3 (es) * 2000-11-13 2007-05-16 Promega Corporation Depuracion de lisado y aislamiento de acido nucleico utilizando matrices de silica silanizadas.
EP1549737A1 (en) * 2002-05-02 2005-07-06 Angiogenetics Sweden AB Isolation of target cells, capillaries and microorgans
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
DE602004016004D1 (de) * 2003-08-26 2008-10-02 Univ Danmarks Tekniske Kontinuierliches verfahren zur anordnung von makromolekulären substanzen und die anschliessende aufnahme und isolierung einer makromolekulären anordnung, sowie ein für dieses verfahren geeignetes system
DE10358137A1 (de) * 2003-12-12 2005-07-07 Merck Patent Gmbh Verfahren und Kit zur Isolierung von RNA
CA2552208A1 (en) 2003-12-30 2005-07-14 3M Innovative Properties Company Detection cartridges, modules, systems and methods
US20060024776A1 (en) * 2004-08-02 2006-02-02 Mcmillian Ray Magnetic particle capture of whole intact organisms from clinical samples
DE102005002343A1 (de) 2005-01-18 2006-07-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur spezifischen oder unspezifischen Separation von Zellen und/oder Viren aus flüssigen Medien und dessen Verwendung
EP1882738A4 (en) * 2005-05-20 2009-02-25 Arkray Inc METHOD FOR OBTAINING MICROORGANISMS AND NUCLEIC ACIDS USING FINE PARTICLES AND FOR THE METHOD OF USING THE KIT
DE102008029356A1 (de) 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
CN104583423A (zh) * 2012-08-30 2015-04-29 凯杰有限公司 确定细胞样品中靶核酸存在或不存在的方法
US20180135040A1 (en) 2016-02-16 2018-05-17 Life Magnetics, Inc. Methods for separating nucleic acids with graphene coated magnetic beads
KR102047362B1 (ko) * 2017-01-16 2019-11-21 (주) 바이오팩트 아민-코팅 자성 나노입자를 이용한 플라스미드 dna 정제 방법 및 조성물
US20220251539A1 (en) * 2019-10-29 2022-08-11 Hewlett-Packard Development Company, L.P. Concentrating biological components

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9003253D0 (en) * 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
US5523231A (en) 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
US5395498A (en) 1991-11-06 1995-03-07 Gombinsky; Moshe Method for separating biological macromolecules and means therfor
DE4307262A1 (de) 1993-03-02 1994-09-08 Christian Bergemann Magnetisches polymeres Siliciumdioxid
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5652348A (en) 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US5660984A (en) 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
DE19512368A1 (de) * 1995-04-01 1996-10-02 Boehringer Mannheim Gmbh System zur Freisetzung und Isolierung von Nukleinsäuren
EP0741141A2 (en) * 1995-05-04 1996-11-06 Hewlett-Packard Company Method of purifying ologonucleotide from biological samples
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

Also Published As

Publication number Publication date
JP4551568B2 (ja) 2010-09-29
WO2000070040A1 (en) 2000-11-23
ATE530648T1 (de) 2011-11-15
AU2398100A (en) 2000-12-05
JP2009118858A (ja) 2009-06-04
AU778486B2 (en) 2004-12-09
JP2002543835A (ja) 2002-12-24
EP1179058A1 (en) 2002-02-13
EP1179058B1 (en) 2011-08-24
ATE521703T1 (de) 2011-09-15

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued