CA2367198A1 - Method of producing and purifying angiostatin protein - Google Patents

Method of producing and purifying angiostatin protein Download PDF

Info

Publication number
CA2367198A1
CA2367198A1 CA002367198A CA2367198A CA2367198A1 CA 2367198 A1 CA2367198 A1 CA 2367198A1 CA 002367198 A CA002367198 A CA 002367198A CA 2367198 A CA2367198 A CA 2367198A CA 2367198 A1 CA2367198 A1 CA 2367198A1
Authority
CA
Canada
Prior art keywords
protein
angiostatin
column
sodium phosphate
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002367198A
Other languages
English (en)
French (fr)
Inventor
John Madsen
Hong Liang
Kim Lee Sim
Xinhua Zhou
Amy Chang-Murad
Renee J. Boerner
Lourdes L. Bermejo
Firoz Rustom Mistry
Jeffrey L. Schrimsher
Scott R. Shepard
Jeremy Johnston
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Entremed Inc
Covance Biotechnology Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Entremed Inc, Covance Biotechnology Services Inc filed Critical Entremed Inc
Publication of CA2367198A1 publication Critical patent/CA2367198A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA002367198A 1999-12-03 2000-12-04 Method of producing and purifying angiostatin protein Abandoned CA2367198A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16891999P 1999-12-03 1999-12-03
US60/168,919 1999-12-03
PCT/US2000/032843 WO2001040260A2 (en) 1999-12-03 2000-12-04 Method of producing and purifying angiostatin

Publications (1)

Publication Number Publication Date
CA2367198A1 true CA2367198A1 (en) 2001-06-07

Family

ID=22613496

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002367198A Abandoned CA2367198A1 (en) 1999-12-03 2000-12-04 Method of producing and purifying angiostatin protein

Country Status (4)

Country Link
EP (1) EP1218495A2 (de)
AU (1) AU781204B2 (de)
CA (1) CA2367198A1 (de)
WO (1) WO2001040260A2 (de)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639725A (en) * 1994-04-26 1997-06-17 Children's Hospital Medical Center Corp. Angiostatin protein
PT1047771E (pt) * 1998-01-12 2003-08-29 Searle & Co Metodo para renaturacao de angiostatina

Also Published As

Publication number Publication date
AU2906101A (en) 2001-06-12
WO2001040260A9 (en) 2002-08-15
WO2001040260A2 (en) 2001-06-07
EP1218495A2 (de) 2002-07-03
AU781204B2 (en) 2005-05-12
WO2001040260A3 (en) 2002-05-02

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Legal Events

Date Code Title Description
FZDE Discontinued