CA2339415C - Increasing stearate content in soybean oil by expression of acyl-acp thiosterase - Google Patents

Increasing stearate content in soybean oil by expression of acyl-acp thiosterase Download PDF

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Publication number
CA2339415C
CA2339415C CA2339415A CA2339415A CA2339415C CA 2339415 C CA2339415 C CA 2339415C CA 2339415 A CA2339415 A CA 2339415A CA 2339415 A CA2339415 A CA 2339415A CA 2339415 C CA2339415 C CA 2339415C
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CA
Canada
Prior art keywords
soybean
plant cell
oil
soybean plant
stearate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2339415A
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English (en)
French (fr)
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CA2339415A1 (en
Inventor
Jean C. Kridl
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Monsanto Technology LLC
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Monsanto Co
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Publication of CA2339415C publication Critical patent/CA2339415C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Edible Oils And Fats (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Fats And Perfumes (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dairy Products (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2339415A 1998-08-14 1999-07-22 Increasing stearate content in soybean oil by expression of acyl-acp thiosterase Expired - Lifetime CA2339415C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/134,262 1998-08-14
US09/134,262 US6365802B2 (en) 1998-08-14 1998-08-14 Methods for increasing stearate content in soybean oil
PCT/US1999/017109 WO2000009721A1 (en) 1998-08-14 1999-07-22 Increasing stearate content in soybean oil by expression of acyl-acp thiosterase

Publications (2)

Publication Number Publication Date
CA2339415A1 CA2339415A1 (en) 2000-02-24
CA2339415C true CA2339415C (en) 2012-06-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2339415A Expired - Lifetime CA2339415C (en) 1998-08-14 1999-07-22 Increasing stearate content in soybean oil by expression of acyl-acp thiosterase

Country Status (7)

Country Link
US (5) US6365802B2 (https=)
EP (1) EP1104477B1 (https=)
JP (1) JP4511043B2 (https=)
AR (1) AR021766A1 (https=)
BR (1) BR9913032A (https=)
CA (1) CA2339415C (https=)
WO (1) WO2000009721A1 (https=)

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Also Published As

Publication number Publication date
US20010002489A1 (en) 2001-05-31
US6365802B2 (en) 2002-04-02
US6380462B1 (en) 2002-04-30
EP1104477B1 (en) 2015-10-28
AR021766A1 (es) 2002-08-07
EP1104477A1 (en) 2001-06-06
CA2339415A1 (en) 2000-02-24
HK1039148A1 (en) 2002-04-12
JP4511043B2 (ja) 2010-07-28
US20090214744A1 (en) 2009-08-27
BR9913032A (pt) 2001-11-27
JP2002522088A (ja) 2002-07-23
US20030070190A1 (en) 2003-04-10
WO2000009721A1 (en) 2000-02-24
US7504563B1 (en) 2009-03-17

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