BR9913032A - Aumento de teor de estearatoem óleo de soja por expressão de acil-acptioesterase - Google Patents

Aumento de teor de estearatoem óleo de soja por expressão de acil-acptioesterase

Info

Publication number
BR9913032A
BR9913032A BR9913032-7A BR9913032A BR9913032A BR 9913032 A BR9913032 A BR 9913032A BR 9913032 A BR9913032 A BR 9913032A BR 9913032 A BR9913032 A BR 9913032A
Authority
BR
Brazil
Prior art keywords
stearate
acyl
soybean
acptioesterase
expression
Prior art date
Application number
BR9913032-7A
Other languages
English (en)
Inventor
Jean C Kridl
Original Assignee
Calgene Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene Llc filed Critical Calgene Llc
Publication of BR9913032A publication Critical patent/BR9913032A/pt

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Edible Oils And Fats (AREA)
  • Fats And Perfumes (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dairy Products (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

Patente de Invenção: "AUMENTO DE TEOR DE ESTEARATOEM óLEO DE SOJA POR EXPRESSãO DE ACIL-ACPTIOESTERASE". Esta invenção refere-se a um método paraaumentar o teor de estearato como um componente dostriglicerídeos totais encontrados na semente de soja. O métodogeralmente compreende cultivar uma planta de soja tendointegrada em seu genoma um construto de DNA compreendendo,na direção de transcrição de 5' para 3', um promotor funcional emuma célula de semente da planta de soja, uma seq³ência de DNAcodificando uma proteína acil-ACP tioesterase tendo atividadesubstancial em substratos de C18:0 acil-ACP, e uma região determinação de transcrição funcional em uma célula vegetal. Apresente invenção também fornece uma semente de soja comcerca de 33 por cento em peso ou mais de estearato como umcomponente dos ácidos graxos totais encontrados em triglicerídeosde sementes.
BR9913032-7A 1998-08-14 1999-07-22 Aumento de teor de estearatoem óleo de soja por expressão de acil-acptioesterase BR9913032A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/134,262 US6365802B2 (en) 1998-08-14 1998-08-14 Methods for increasing stearate content in soybean oil
PCT/US1999/017109 WO2000009721A1 (en) 1998-08-14 1999-07-22 Increasing stearate content in soybean oil by expression of acyl-acp thiosterase

Publications (1)

Publication Number Publication Date
BR9913032A true BR9913032A (pt) 2001-11-27

Family

ID=22462531

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9913032-7A BR9913032A (pt) 1998-08-14 1999-07-22 Aumento de teor de estearatoem óleo de soja por expressão de acil-acptioesterase

Country Status (8)

Country Link
US (5) US6365802B2 (pt)
EP (1) EP1104477B1 (pt)
JP (1) JP4511043B2 (pt)
AR (1) AR021766A1 (pt)
BR (1) BR9913032A (pt)
CA (1) CA2339415C (pt)
HK (1) HK1039148A1 (pt)
WO (1) WO2000009721A1 (pt)

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Also Published As

Publication number Publication date
AR021766A1 (es) 2002-08-07
US20030070190A1 (en) 2003-04-10
HK1039148A1 (en) 2002-04-12
US6365802B2 (en) 2002-04-02
US20090214744A1 (en) 2009-08-27
US20010002489A1 (en) 2001-05-31
JP4511043B2 (ja) 2010-07-28
EP1104477B1 (en) 2015-10-28
CA2339415A1 (en) 2000-02-24
WO2000009721A1 (en) 2000-02-24
JP2002522088A (ja) 2002-07-23
US6380462B1 (en) 2002-04-30
CA2339415C (en) 2012-06-19
EP1104477A1 (en) 2001-06-06
US7504563B1 (en) 2009-03-17

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