CA2334079A1 - Genes for the biosynthesis of anticapsin and bacilysine, their isolation and their use - Google Patents
Genes for the biosynthesis of anticapsin and bacilysine, their isolation and their use Download PDFInfo
- Publication number
- CA2334079A1 CA2334079A1 CA002334079A CA2334079A CA2334079A1 CA 2334079 A1 CA2334079 A1 CA 2334079A1 CA 002334079 A CA002334079 A CA 002334079A CA 2334079 A CA2334079 A CA 2334079A CA 2334079 A1 CA2334079 A1 CA 2334079A1
- Authority
- CA
- Canada
- Prior art keywords
- bacilysin
- genes
- plasmid
- bac
- bacillus subtilis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 98
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 92
- KHVZXXWDPSCGEK-UHFFFAOYSA-N Anticapsin Natural products OC(=O)C(N)CC1CCC(=O)C2OC12 KHVZXXWDPSCGEK-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 238000002955 isolation Methods 0.000 title claims abstract description 8
- KHVZXXWDPSCGEK-ROHCDXGRSA-N (2s)-2-amino-3-[(1r,2r,6r)-5-oxo-7-oxabicyclo[4.1.0]heptan-2-yl]propanoic acid Chemical compound OC(=O)[C@@H](N)C[C@H]1CCC(=O)[C@@H]2O[C@H]12 KHVZXXWDPSCGEK-ROHCDXGRSA-N 0.000 title claims abstract 7
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 16
- 238000003786 synthesis reaction Methods 0.000 claims abstract description 15
- XFOUAXMJRHNTOP-ZABDLSDTSA-N (2s)-2-[[(2s)-2-aminopropanoyl]amino]-3-[(1r,2r,6r)-5-oxo-7-oxabicyclo[4.1.0]heptan-2-yl]propanoic acid Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)C[C@H]1CCC(=O)[C@@H]2O[C@H]12 XFOUAXMJRHNTOP-ZABDLSDTSA-N 0.000 claims description 131
- XFOUAXMJRHNTOP-UHFFFAOYSA-N Bacilysin Natural products CC(N)C(=O)NC(C(O)=O)CC1CCC(=O)C2OC12 XFOUAXMJRHNTOP-UHFFFAOYSA-N 0.000 claims description 130
- 108700023668 bacilysin Proteins 0.000 claims description 130
- 239000013612 plasmid Substances 0.000 claims description 56
- 241000276408 Bacillus subtilis subsp. subtilis str. 168 Species 0.000 claims description 35
- 238000000034 method Methods 0.000 claims description 25
- 238000010367 cloning Methods 0.000 claims description 23
- 101150022095 bacB gene Proteins 0.000 claims description 22
- 238000003780 insertion Methods 0.000 claims description 21
- 230000037431 insertion Effects 0.000 claims description 21
- 244000063299 Bacillus subtilis Species 0.000 claims description 20
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 20
- 108020004414 DNA Proteins 0.000 claims description 19
- 101100539529 Bacillus thuringiensis subsp. konkukian (strain 97-27) uppP2 gene Proteins 0.000 claims description 17
- 101100155460 Bacillus thuringiensis subsp. konkukian (strain 97-27) uppP3 gene Proteins 0.000 claims description 17
- 101100155465 Bacillus thuringiensis subsp. konkukian (strain 97-27) uppP4 gene Proteins 0.000 claims description 17
- 101150084317 bacA gene Proteins 0.000 claims description 17
- 101150103473 bacE gene Proteins 0.000 claims description 17
- 101150020973 uppP gene Proteins 0.000 claims description 17
- 101150058431 uppP1 gene Proteins 0.000 claims description 17
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 16
- 239000012634 fragment Substances 0.000 claims description 15
- 101100165124 Bacillus subtilis (strain 168) bacF gene Proteins 0.000 claims description 14
- 230000005764 inhibitory process Effects 0.000 claims description 13
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 12
- 239000013600 plasmid vector Substances 0.000 claims description 12
- 238000003776 cleavage reaction Methods 0.000 claims description 10
- 230000007017 scission Effects 0.000 claims description 10
- 239000013598 vector Substances 0.000 claims description 10
- 101150078992 bacC gene Proteins 0.000 claims description 8
- 230000012010 growth Effects 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 239000013611 chromosomal DNA Substances 0.000 claims description 7
- 108091008146 restriction endonucleases Proteins 0.000 claims description 7
- 230000000087 stabilizing effect Effects 0.000 claims description 7
- 230000009466 transformation Effects 0.000 claims description 7
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 6
- 241001522878 Escherichia coli B Species 0.000 claims description 5
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 claims description 5
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 claims description 5
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 claims description 5
- 108700026244 Open Reading Frames Proteins 0.000 claims description 5
- 229950006780 n-acetylglucosamine Drugs 0.000 claims description 5
- 241000194103 Bacillus pumilus Species 0.000 claims description 4
- 241000588767 Proteus vulgaris Species 0.000 claims description 4
- -1 aromatic amino acids Chemical class 0.000 claims description 4
- 238000012261 overproduction Methods 0.000 claims description 4
- 229940007042 proteus vulgaris Drugs 0.000 claims description 4
- 101100165122 Bacillus subtilis (strain 168) bacE gene Proteins 0.000 claims description 3
- 101100268025 Bacillus subtilis (strain 168) ywfA gene Proteins 0.000 claims description 3
- 206010061765 Chromosomal mutation Diseases 0.000 claims description 3
- 230000004544 DNA amplification Effects 0.000 claims description 3
- 101100268026 Lactococcus lactis subsp. lactis (strain IL1403) ywfF gene Proteins 0.000 claims description 3
- 230000002269 spontaneous effect Effects 0.000 claims description 3
- 238000001712 DNA sequencing Methods 0.000 claims description 2
- 230000000295 complement effect Effects 0.000 claims description 2
- 230000002068 genetic effect Effects 0.000 claims description 2
- 101100111169 Bacillus subtilis (strain 168) bacA gene Proteins 0.000 claims 1
- 101100325727 Bacillus subtilis (strain 168) bacB gene Proteins 0.000 claims 1
- 101100325731 Bacillus subtilis (strain 168) bacC gene Proteins 0.000 claims 1
- 101100165111 Bacillus subtilis (strain 168) bacD gene Proteins 0.000 claims 1
- 108010077805 Bacterial Proteins Proteins 0.000 claims 1
- 230000002950 deficient Effects 0.000 claims 1
- 238000009776 industrial production Methods 0.000 claims 1
- 230000003115 biocidal effect Effects 0.000 abstract description 11
- 244000000010 microbial pathogen Species 0.000 abstract description 5
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 abstract description 3
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 3
- 229960002442 glucosamine Drugs 0.000 abstract description 3
- 239000005557 antagonist Substances 0.000 abstract description 2
- KHVZXXWDPSCGEK-MGVQOFIGSA-N anticapsin zwitterion Chemical compound OC(=O)[C@@H](N)C[C@@H]1CCC(=O)[C@@H]2O[C@H]12 KHVZXXWDPSCGEK-MGVQOFIGSA-N 0.000 description 33
- 230000002759 chromosomal effect Effects 0.000 description 18
- FPWMCUPFBRFMLH-UHFFFAOYSA-N prephenic acid Chemical compound OC1C=CC(CC(=O)C(O)=O)(C(O)=O)C=C1 FPWMCUPFBRFMLH-UHFFFAOYSA-N 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 16
- 230000035772 mutation Effects 0.000 description 14
- 238000012217 deletion Methods 0.000 description 13
- 230000037430 deletion Effects 0.000 description 13
- 229920001817 Agar Polymers 0.000 description 10
- 230000010354 integration Effects 0.000 description 10
- 235000010419 agar Nutrition 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 239000008272 agar Substances 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 7
- 101100496432 Streptomyces lividans cmlR gene Proteins 0.000 description 7
- 229960003767 alanine Drugs 0.000 description 7
- 229940024606 amino acid Drugs 0.000 description 7
- 235000001014 amino acid Nutrition 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 235000018102 proteins Nutrition 0.000 description 7
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 6
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 6
- 239000003242 anti bacterial agent Substances 0.000 description 6
- 229940088710 antibiotic agent Drugs 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 101150017091 bacD gene Proteins 0.000 description 5
- 239000012228 culture supernatant Substances 0.000 description 5
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 4
- 230000006037 cell lysis Effects 0.000 description 4
- 229960005091 chloramphenicol Drugs 0.000 description 4
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 4
- 230000007547 defect Effects 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 101150058765 BACE1 gene Proteins 0.000 description 3
- 241000193749 Bacillus coagulans Species 0.000 description 3
- 241000194107 Bacillus megaterium Species 0.000 description 3
- CDXAUQAPYPMADZ-UHFFFAOYSA-N Chlorotetaine Natural products CC(N)NC(=O)C(CC1CCC(=O)C(=C1)Cl)C(=O)O CDXAUQAPYPMADZ-UHFFFAOYSA-N 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 229940054340 bacillus coagulans Drugs 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 3
- 108700029774 chlorotetaine Proteins 0.000 description 3
- 239000013599 cloning vector Substances 0.000 description 3
- 101150051570 cmlR gene Proteins 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- 230000029142 excretion Effects 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000004807 localization Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000002703 mutagenesis Methods 0.000 description 3
- 231100000350 mutagenesis Toxicity 0.000 description 3
- 150000007523 nucleic acids Chemical group 0.000 description 3
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 241000194108 Bacillus licheniformis Species 0.000 description 2
- 241000195576 Bacillus subtilis group Species 0.000 description 2
- 108020003338 D-alanine-D-alanine ligase Proteins 0.000 description 2
- 108010016626 Dipeptides Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 241000206672 Gelidium Species 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- 101100095205 Lactococcus lactis subsp. lactis scrB gene Proteins 0.000 description 2
- 230000001594 aberrant effect Effects 0.000 description 2
- 238000012459 agar diffusion assay Methods 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 101150059761 ctrA gene Proteins 0.000 description 2
- 229960003276 erythromycin Drugs 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 238000007169 ligase reaction Methods 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 101150054232 pyrG gene Proteins 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 101150082821 sacA gene Proteins 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 238000010361 transduction Methods 0.000 description 2
- 230000026683 transduction Effects 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 108010054473 Bacteria oligopeptide permease Proteins 0.000 description 1
- 241000123650 Botrytis cinerea Species 0.000 description 1
- 101000583086 Bunodosoma granuliferum Delta-actitoxin-Bgr2b Proteins 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 241001646716 Escherichia coli K-12 Species 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000004894 Glutamine-fructose-6-phosphate transaminase (isomerizing) Human genes 0.000 description 1
- 108090001031 Glutamine-fructose-6-phosphate transaminase (isomerizing) Proteins 0.000 description 1
- 241000606768 Haemophilus influenzae Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 238000002768 Kirby-Bauer method Methods 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000301 Membrane transport proteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 108010015724 Prephenate Dehydratase Proteins 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 241000588770 Proteus mirabilis Species 0.000 description 1
- 241000607132 Salmonella enterica subsp. enterica serovar Gallinarum Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000193996 Streptococcus pyogenes Species 0.000 description 1
- 241000187432 Streptomyces coelicolor Species 0.000 description 1
- 241000936710 Streptomyces griseoplanus Species 0.000 description 1
- 206010042674 Swelling Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 210000003578 bacterial chromosome Anatomy 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
- 238000013452 biotechnological production Methods 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000006957 competitive inhibition Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 108091008053 gene clusters Proteins 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000002337 glycosamines Chemical class 0.000 description 1
- 229940047650 haemophilus influenzae Drugs 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 108010082406 peptide permease Proteins 0.000 description 1
- 235000020030 perry Nutrition 0.000 description 1
- 230000003032 phytopathogenic effect Effects 0.000 description 1
- 230000008659 phytopathology Effects 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 244000045561 useful plants Species 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19830912.0 | 1998-07-10 | ||
| DE19830912 | 1998-07-10 | ||
| DE19921807.2 | 1999-05-11 | ||
| DE19921807 | 1999-05-11 | ||
| PCT/DE1999/002179 WO2000003009A2 (de) | 1998-07-10 | 1999-07-12 | Gene zur biosynthese von anticapsin und bacilysin, ihre isolierung und verwendung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2334079A1 true CA2334079A1 (en) | 2000-01-20 |
Family
ID=26047325
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002334079A Abandoned CA2334079A1 (en) | 1998-07-10 | 1999-07-12 | Genes for the biosynthesis of anticapsin and bacilysine, their isolation and their use |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1097214A2 (de) |
| AU (1) | AU6077499A (de) |
| CA (1) | CA2334079A1 (de) |
| DE (1) | DE19932908A1 (de) |
| WO (1) | WO2000003009A2 (de) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7514242B2 (en) | 2002-12-26 | 2009-04-07 | Kyowa Hakko Kogyo Co., Ltd. | Process for producing dipeptides |
| US7939302B2 (en) | 2004-06-25 | 2011-05-10 | Kyowa Hakko Bio Co., Ltd. | Process for producing dipeptides |
| US8709752B2 (en) | 2004-06-25 | 2014-04-29 | Kyowa Hakko Bio Co., Ltd. | Process for producing dipeptides or dipeptide derivatives |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006001381A1 (ja) * | 2004-06-25 | 2006-01-05 | Kyowa Hakko Kogyo Co., Ltd. | ジペプチドまたはジペプチド誘導体の製造法 |
| EP2179652B1 (de) | 2008-09-10 | 2019-05-08 | ABiTEP GmbH Gesellschaft für AgroBioTechnische Entwicklung und Produktion | Verwendung eines antibakteriellen Mittels zur Behandlung von bakteriellen Infektionen bei Kulturpflanzen |
| CN112226437B (zh) * | 2020-10-26 | 2022-02-15 | 湖北大学 | 梯度调控芽胞杆菌启动子启动效率的序列组合及应用 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19654841A1 (de) * | 1996-12-31 | 1998-07-02 | Inst Pflanzengenetik & Kultur | Zweifach positiv selektive Klonierungsvektoren |
-
1999
- 1999-07-12 WO PCT/DE1999/002179 patent/WO2000003009A2/de not_active Application Discontinuation
- 1999-07-12 CA CA002334079A patent/CA2334079A1/en not_active Abandoned
- 1999-07-12 DE DE19932908A patent/DE19932908A1/de not_active Withdrawn
- 1999-07-12 AU AU60774/99A patent/AU6077499A/en not_active Abandoned
- 1999-07-12 EP EP99947209A patent/EP1097214A2/de not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7514242B2 (en) | 2002-12-26 | 2009-04-07 | Kyowa Hakko Kogyo Co., Ltd. | Process for producing dipeptides |
| US8039236B2 (en) | 2002-12-26 | 2011-10-18 | Kyowa Hakko Bio Co., Ltd. | Process for producing dipeptides |
| US7939302B2 (en) | 2004-06-25 | 2011-05-10 | Kyowa Hakko Bio Co., Ltd. | Process for producing dipeptides |
| US8709752B2 (en) | 2004-06-25 | 2014-04-29 | Kyowa Hakko Bio Co., Ltd. | Process for producing dipeptides or dipeptide derivatives |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1097214A2 (de) | 2001-05-09 |
| AU6077499A (en) | 2000-02-01 |
| DE19932908A1 (de) | 2000-01-20 |
| WO2000003009A2 (de) | 2000-01-20 |
| WO2000003009A3 (de) | 2000-05-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Martin et al. | Cross‐regulation of competence pheromone production and export in the early control of transformation in Streptococcus pneumoniae | |
| US6207384B1 (en) | Systematic identification of essential genes by in vitro transposon mutagenesis | |
| McLaughlan et al. | Molecular characterization of an autolytic amidase of Listeria monocytogenes EGD | |
| JPH11509096A (ja) | 胞子形成できないバチルスを用いるタンパク質の生産 | |
| EP0830457A1 (de) | Verfahren zur auffindung von antimikrobiellen zielen | |
| O'Connell et al. | Sequence and mapping of the aroA gene of Staphylococcus aureus 8325-4 | |
| Liu et al. | Plasmid-amplified comS enhances genetic competence and suppresses sinR in Bacillus subtilis | |
| US6136587A (en) | Auxiliary genes and proteins of methicillin resistant bacteria and antagonists thereof | |
| CA2334079A1 (en) | Genes for the biosynthesis of anticapsin and bacilysine, their isolation and their use | |
| JPH03500606A (ja) | プロテアーゼ欠損グラム陽性菌類及び組換え体産生用宿主有機体としての用途 | |
| Nakamura et al. | Complementation of the protein transport defect of an Escherichia coli sec Y mutant (sec Y24) by Bacillus subtilis sec Y homologue | |
| JP2003509010A (ja) | ペプチドのスクリーニング方法 | |
| US20230101904A1 (en) | Gram-positive bacteria of the species lactococcus lactis or streptococcus thermophilus having a very low surface proteolysis, processes for obtaining them and uses thereof | |
| Köroğlu et al. | The novel gene yvfI in Bacillus subtilis is essential for bacilysin biosynthesis | |
| Schrögel et al. | Expression of a pepT homologue from Bacillus subtilis | |
| Fu et al. | Fengycin controls the apple blue disease caused by Penicillium expansum. | |
| US20110020869A1 (en) | Methods for altering acetic acid production and enhancing cell death in bacteria | |
| JP2002511239A (ja) | スクリーニングのための調節された標的発現 | |
| Wang et al. | Three non-aspartate amino acid mutations in the ComA response regulator receiver motif severely decrease surfactin production, competence development, and spore formation in Bacillus subtilis | |
| Buchholz et al. | yneA mRNA instability is involved in temporary inhibition of cell division during the SOS response of Bacillus megaterium | |
| EP1472347B1 (de) | Verbesserte proteinexpression in bacillus subtilis | |
| Ramakrishnan et al. | Partial characterization and cloning of protease from Bacillus | |
| Buurman et al. | Regulation of both gene expression and protein stability provides genetically assisted target evaluation (GATE) for microbial target validation | |
| Kim et al. | Operon required for fruiting body development in Myxococcus xanthus | |
| Hartzell | Genetic tools for dissecting motility and development of Myxococcus xanthus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |