CA2329031C - High numerical aperture flow cytometer and method of using same - Google Patents
High numerical aperture flow cytometer and method of using same Download PDFInfo
- Publication number
- CA2329031C CA2329031C CA002329031A CA2329031A CA2329031C CA 2329031 C CA2329031 C CA 2329031C CA 002329031 A CA002329031 A CA 002329031A CA 2329031 A CA2329031 A CA 2329031A CA 2329031 C CA2329031 C CA 2329031C
- Authority
- CA
- Canada
- Prior art keywords
- light
- scattered
- flow cytometer
- laser
- light detector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims description 21
- 210000004027 cell Anatomy 0.000 claims abstract description 52
- 210000000601 blood cell Anatomy 0.000 claims abstract description 19
- 210000003979 eosinophil Anatomy 0.000 claims description 31
- 239000002245 particle Substances 0.000 claims description 24
- 239000012530 fluid Substances 0.000 claims description 19
- 210000000265 leukocyte Anatomy 0.000 claims description 12
- 241000700605 Viruses Species 0.000 claims description 2
- 239000010453 quartz Substances 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 239000004065 semiconductor Substances 0.000 claims 1
- 241000897276 Termes Species 0.000 abstract 1
- 230000010287 polarization Effects 0.000 description 12
- 210000004369 blood Anatomy 0.000 description 9
- 239000008280 blood Substances 0.000 description 9
- 238000000926 separation method Methods 0.000 description 7
- 241000282465 Canis Species 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 230000002934 lysing effect Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- NWXMGUDVXFXRIG-NAOJVREFSA-N (4s,4as,5as,12ar)-4-(dimethylamino)-1,6,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide Chemical compound C1=CC=C2C(O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(=O)C(C(N)=O)=C(O)[C@@]4(O)C(=O)C3=C(O)C2=C1O NWXMGUDVXFXRIG-NAOJVREFSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 206010012289 Dementia Diseases 0.000 description 1
- 241001233242 Lontra Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241001274197 Scatophagus argus Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000005862 Whey Substances 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000028161 membrane depolarization Effects 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229910000028 potassium bicarbonate Inorganic materials 0.000 description 1
- 235000015497 potassium bicarbonate Nutrition 0.000 description 1
- 239000011736 potassium bicarbonate Substances 0.000 description 1
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/016—White blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N2015/1447—Spatial selection
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12080499P | 1999-02-19 | 1999-02-19 | |
| US60/120,804 | 1999-02-19 | ||
| PCT/US2000/004069 WO2000049387A2 (en) | 1999-02-19 | 2000-02-18 | High numerical aperture flow cytometer and method of using same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2329031A1 CA2329031A1 (en) | 2000-08-24 |
| CA2329031C true CA2329031C (en) | 2003-09-23 |
Family
ID=22392650
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002329031A Expired - Fee Related CA2329031C (en) | 1999-02-19 | 2000-02-18 | High numerical aperture flow cytometer and method of using same |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1095256A2 (enExample) |
| JP (1) | JP2002537557A (enExample) |
| AU (1) | AU768616C (enExample) |
| CA (1) | CA2329031C (enExample) |
| WO (1) | WO2000049387A2 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69830598T2 (de) | 1997-01-31 | 2006-05-18 | The Horticulture And Food Research Institute Of New Zealand Limited | Optische vorrichtung und methode |
| US6071689A (en) | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
| US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| US6618143B2 (en) * | 2000-02-18 | 2003-09-09 | Idexx Laboratories, Inc. | High numerical aperture flow cytometer and method of using same |
| AU2002237689B2 (en) | 2000-11-29 | 2008-01-10 | Xy, Llc. | System to separate frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations |
| US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
| DK2308419T3 (da) | 2003-03-28 | 2016-06-06 | Inguran Llc | Fremgangsmåde til bedømmelse af farvningstilstandene for dyresperm, som skal sorteres. |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4818103A (en) * | 1981-05-15 | 1989-04-04 | Ratcom | Flow cytometry |
| US4606636A (en) * | 1983-10-25 | 1986-08-19 | Universite De Saint-Etienne | Optical apparatus for identifying the individual multiparametric properties of particles or bodies in a continuous flow |
| NL8601000A (nl) * | 1986-04-21 | 1987-11-16 | Jan Greve T H Twente Afdeling | Het gebruik van gepolariseerd licht in stromingscytometrie. |
| US5057413A (en) * | 1988-06-13 | 1991-10-15 | Becton, Dickinson And Company | Method for discriminating between intact and damaged cells in a sample |
| US4954715A (en) * | 1989-06-26 | 1990-09-04 | Zoeld Tibor | Method and apparatus for an optimized multiparameter flow-through particle and cell analyzer |
| US5631165A (en) * | 1994-08-01 | 1997-05-20 | Abbott Laboratories | Method for performing automated hematology and cytometry analysis |
| ES2251722T3 (es) * | 1994-08-01 | 2006-05-01 | Abbott Laboratories | Procedimiento y aparato para la realizacion de analisis automatizados. |
| JP3324050B2 (ja) * | 1994-10-31 | 2002-09-17 | 日本光電工業株式会社 | 白血球分類用試薬および白血球分類方法 |
| US5650847A (en) * | 1995-06-14 | 1997-07-22 | Erkki Soini | Method and device for determination of parameters of individual microparticles |
| JP3504029B2 (ja) * | 1995-07-04 | 2004-03-08 | シスメックス株式会社 | 粒子分析装置 |
| EP0805441B1 (de) * | 1996-05-03 | 2003-10-08 | Ciba SC Holding AG | Optische Speichermedien mit hoher Kapazität, die Xanthenfarbstoffe enthalten |
| DE19700648A1 (de) * | 1997-01-10 | 1998-07-23 | Basf Ag | Verfahren und Vorrichtung zur Bestimmung der Größenverteilung von verschiedenartigen Partikeln in einer Probe |
| JP3642658B2 (ja) * | 1997-06-30 | 2005-04-27 | シスメックス株式会社 | 尿中有形成分分析装置および分析方法 |
-
2000
- 2000-02-18 WO PCT/US2000/004069 patent/WO2000049387A2/en not_active Ceased
- 2000-02-18 AU AU35976/00A patent/AU768616C/en not_active Ceased
- 2000-02-18 CA CA002329031A patent/CA2329031C/en not_active Expired - Fee Related
- 2000-02-18 EP EP00914609A patent/EP1095256A2/en not_active Ceased
- 2000-02-18 JP JP2000600079A patent/JP2002537557A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU3597600A (en) | 2000-09-04 |
| AU768616B2 (en) | 2003-12-18 |
| CA2329031A1 (en) | 2000-08-24 |
| JP2002537557A (ja) | 2002-11-05 |
| AU768616C (en) | 2004-12-16 |
| WO2000049387A9 (en) | 2001-09-27 |
| WO2000049387A2 (en) | 2000-08-24 |
| WO2000049387A3 (en) | 2001-02-15 |
| EP1095256A2 (en) | 2001-05-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20180219 |