AU768616C - High numerical aperture flow cytometer and method of using same - Google Patents
High numerical aperture flow cytometer and method of using sameInfo
- Publication number
- AU768616C AU768616C AU35976/00A AU3597600A AU768616C AU 768616 C AU768616 C AU 768616C AU 35976/00 A AU35976/00 A AU 35976/00A AU 3597600 A AU3597600 A AU 3597600A AU 768616 C AU768616 C AU 768616C
- Authority
- AU
- Australia
- Prior art keywords
- light
- scattered
- light detector
- flow cytometer
- laser
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000000034 method Methods 0.000 title claims description 20
- 210000004027 cell Anatomy 0.000 claims description 43
- 210000003979 eosinophil Anatomy 0.000 claims description 32
- 239000002245 particle Substances 0.000 claims description 18
- 210000000601 blood cell Anatomy 0.000 claims description 16
- 239000012530 fluid Substances 0.000 claims description 11
- 210000000265 leukocyte Anatomy 0.000 claims description 10
- 241000700605 Viruses Species 0.000 claims description 2
- 239000010453 quartz Substances 0.000 claims description 2
- 239000004065 semiconductor Substances 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 230000010287 polarization Effects 0.000 description 12
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 241000282465 Canis Species 0.000 description 7
- 238000000926 separation method Methods 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 3
- 238000012512 characterization method Methods 0.000 description 3
- 230000002934 lysing effect Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000028161 membrane depolarization Effects 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229910000028 potassium bicarbonate Inorganic materials 0.000 description 1
- 235000015497 potassium bicarbonate Nutrition 0.000 description 1
- 239000011736 potassium bicarbonate Substances 0.000 description 1
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/016—White blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N2015/1447—Spatial selection
Landscapes
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Dispersion Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12080499P | 1999-02-19 | 1999-02-19 | |
| US60/120804 | 1999-02-19 | ||
| PCT/US2000/004069 WO2000049387A2 (en) | 1999-02-19 | 2000-02-18 | High numerical aperture flow cytometer and method of using same |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| AU3597600A AU3597600A (en) | 2000-09-04 |
| AU768616B2 AU768616B2 (en) | 2003-12-18 |
| AU768616C true AU768616C (en) | 2004-12-16 |
Family
ID=22392650
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU35976/00A Ceased AU768616C (en) | 1999-02-19 | 2000-02-18 | High numerical aperture flow cytometer and method of using same |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1095256A2 (enExample) |
| JP (1) | JP2002537557A (enExample) |
| AU (1) | AU768616C (enExample) |
| CA (1) | CA2329031C (enExample) |
| WO (1) | WO2000049387A2 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2264428B1 (en) | 1997-01-31 | 2017-05-03 | Xy, Llc | Optical apparatus with focussing reflector for converging radiation onto a flow of particles |
| US6071689A (en) | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
| US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| US6618143B2 (en) * | 2000-02-18 | 2003-09-09 | Idexx Laboratories, Inc. | High numerical aperture flow cytometer and method of using same |
| AU2002237689B2 (en) | 2000-11-29 | 2008-01-10 | Xy, Llc. | System to separate frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations |
| US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
| EP2305171B1 (en) | 2003-03-28 | 2021-12-29 | Inguran, LLC | Apparatus and methods for providing sex-sorted animal sperm |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5650847A (en) * | 1995-06-14 | 1997-07-22 | Erkki Soini | Method and device for determination of parameters of individual microparticles |
| US5747343A (en) * | 1994-10-31 | 1998-05-05 | Nihon Kohden Corporation | Leukocyte classification reagent |
| US5940177A (en) * | 1997-01-10 | 1999-08-17 | Basf Aktiengesellschaft | Method and apparatus for determining the size distribution of different types of particles in a sample |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4818103A (en) * | 1981-05-15 | 1989-04-04 | Ratcom | Flow cytometry |
| US4606636A (en) * | 1983-10-25 | 1986-08-19 | Universite De Saint-Etienne | Optical apparatus for identifying the individual multiparametric properties of particles or bodies in a continuous flow |
| NL8601000A (nl) * | 1986-04-21 | 1987-11-16 | Jan Greve T H Twente Afdeling | Het gebruik van gepolariseerd licht in stromingscytometrie. |
| US5057413A (en) * | 1988-06-13 | 1991-10-15 | Becton, Dickinson And Company | Method for discriminating between intact and damaged cells in a sample |
| US4954715A (en) * | 1989-06-26 | 1990-09-04 | Zoeld Tibor | Method and apparatus for an optimized multiparameter flow-through particle and cell analyzer |
| DE69532045T2 (de) * | 1994-08-01 | 2004-07-08 | Abbott Laboratories, Abbott Park | Verfahren und Vorrichtung zur Vorbereitung einer Flüssigkeit |
| US5631165A (en) * | 1994-08-01 | 1997-05-20 | Abbott Laboratories | Method for performing automated hematology and cytometry analysis |
| JP3504029B2 (ja) * | 1995-07-04 | 2004-03-08 | シスメックス株式会社 | 粒子分析装置 |
| EP0805441B1 (de) * | 1996-05-03 | 2003-10-08 | Ciba SC Holding AG | Optische Speichermedien mit hoher Kapazität, die Xanthenfarbstoffe enthalten |
| JP3642658B2 (ja) * | 1997-06-30 | 2005-04-27 | シスメックス株式会社 | 尿中有形成分分析装置および分析方法 |
-
2000
- 2000-02-18 AU AU35976/00A patent/AU768616C/en not_active Ceased
- 2000-02-18 JP JP2000600079A patent/JP2002537557A/ja active Pending
- 2000-02-18 CA CA002329031A patent/CA2329031C/en not_active Expired - Fee Related
- 2000-02-18 WO PCT/US2000/004069 patent/WO2000049387A2/en not_active Ceased
- 2000-02-18 EP EP00914609A patent/EP1095256A2/en not_active Ceased
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5747343A (en) * | 1994-10-31 | 1998-05-05 | Nihon Kohden Corporation | Leukocyte classification reagent |
| US5650847A (en) * | 1995-06-14 | 1997-07-22 | Erkki Soini | Method and device for determination of parameters of individual microparticles |
| US5940177A (en) * | 1997-01-10 | 1999-08-17 | Basf Aktiengesellschaft | Method and apparatus for determining the size distribution of different types of particles in a sample |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2329031C (en) | 2003-09-23 |
| AU3597600A (en) | 2000-09-04 |
| WO2000049387A3 (en) | 2001-02-15 |
| AU768616B2 (en) | 2003-12-18 |
| EP1095256A2 (en) | 2001-05-02 |
| CA2329031A1 (en) | 2000-08-24 |
| WO2000049387A2 (en) | 2000-08-24 |
| WO2000049387A9 (en) | 2001-09-27 |
| JP2002537557A (ja) | 2002-11-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| DA2 | Applications for amendment section 104 |
Free format text: THE NATURE OF THE PROPOSED AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 20040429 |