CA2318662A1 - Procede de determination de la fonction de sequences nucleotidiques et les proteines pour lesquelles elles codent par transfection de telles sequences dans un hote - Google Patents

Procede de determination de la fonction de sequences nucleotidiques et les proteines pour lesquelles elles codent par transfection de telles sequences dans un hote Download PDF

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Publication number
CA2318662A1
CA2318662A1 CA002318662A CA2318662A CA2318662A1 CA 2318662 A1 CA2318662 A1 CA 2318662A1 CA 002318662 A CA002318662 A CA 002318662A CA 2318662 A CA2318662 A CA 2318662A CA 2318662 A1 CA2318662 A1 CA 2318662A1
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CA
Canada
Prior art keywords
nucleic acid
plant
viral
sequence
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002318662A
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English (en)
Inventor
Guy Della-Cioppa
Robert L. Erwin
Wayne P. Fitzmaurice
Kathleen M. Hanley
Monto H. Kumagai
John A. Lindbo
David R. Mcgee
Hal S. Padgett
Gregory P. Pogue
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Large Scale Biology Corp
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2318662A1 publication Critical patent/CA2318662A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La présente invention concerne des procédés de détermination rapide de la fonction de séquences d'acides nucléiques qui consiste en la transfection desdites séquences dans un organisme hôte afin d'y effectuer une expression; ensuite à analyser les changements phénotypiques et biochimiques ainsi produits afin de déterminer la fonction des acides nucléiques transfectés dans l'organisme hôte. L'invention concerne aussi des procédés de blocage des gènes endogènes par la transfection des hôtes ayant des séquences d'acides nucléiques pour en effectuer l'expression. L'invention concerne en outre des procédés de sélection des fonctions désirées des ARN et des protéines au moyen de vecteurs viraux en vue d'exprimer des banques des allèles des séquences d'acides nucléiques. Finalement l'invention concerne des procédés d'inhibition d'une protéase endogène d'une plante hôte.
CA002318662A 1998-01-16 1999-01-15 Procede de determination de la fonction de sequences nucleotidiques et les proteines pour lesquelles elles codent par transfection de telles sequences dans un hote Abandoned CA2318662A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US818698A 1998-01-16 1998-01-16
US09/008,186 1998-01-16
PCT/US1999/001164 WO1999036516A2 (fr) 1998-01-16 1999-01-15 Procede de determination de la fonction de sequences nucleotidiques et les proteines pour lesquelles elles codent par transfection de telles sequences dans un hote

Publications (1)

Publication Number Publication Date
CA2318662A1 true CA2318662A1 (fr) 1999-07-22

Family

ID=21730222

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002318662A Abandoned CA2318662A1 (fr) 1998-01-16 1999-01-15 Procede de determination de la fonction de sequences nucleotidiques et les proteines pour lesquelles elles codent par transfection de telles sequences dans un hote

Country Status (6)

Country Link
EP (1) EP1045899A2 (fr)
JP (1) JP2002508957A (fr)
KR (1) KR20010040337A (fr)
AU (1) AU761367B2 (fr)
CA (1) CA2318662A1 (fr)
WO (1) WO1999036516A2 (fr)

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US6300134B1 (en) 1998-01-16 2001-10-09 Large Scale Biology Corporation RNA transformation vectors derived from a single-component RNA virus and contain an intervening sequence between the cap and the 5′ end
US6468745B1 (en) 1998-01-16 2002-10-22 Large Scale Biology Corporation Method for expressing a library of nucleic acid sequence variants and selecting desired traits
US6303848B1 (en) 1998-01-16 2001-10-16 Large Scale Biology Corporation Method for conferring herbicide, pest, or disease resistance in plant hosts
US6700040B2 (en) * 1998-01-16 2004-03-02 Large Scale Biology Corporation Cytoplasmic gene inhibition or gene expression in transfected plants by a tobraviral vector
US6300133B1 (en) 1998-01-16 2001-10-09 Large Scale Biology Corporation RNA transformation vectors derived from an uncapped single-component RNA virus
WO2001007600A1 (fr) * 1999-07-21 2001-02-01 Large Scale Biology Corporation Procede de correlation de fonction de sequence par transfection en orientation antisens ou en sens positif dans un hote vegetal d'une sequence d'acide nucleique d'un organisme donneur
WO2000063397A2 (fr) * 1999-04-20 2000-10-26 Aventis Cropscience N.V. Methodes et moyens d'administration d'arn inhibiteur a des vegetaux et applications associees
IL147343A0 (en) * 1999-07-21 2002-08-14 Large Scale Biology Corp Method for enhancing ran or protein production using non-native 5' untranslated sequences in recombinant viral nucleic acids
AU1800401A (en) * 1999-11-23 2001-06-04 Maxygen, Inc. Shuffling of agrobacterium and viral genes, plasmids and genomes for improved plant transformation
WO2001059071A2 (fr) 2000-02-09 2001-08-16 Genvec, Inc. Methodes de preparation et d'utilisation d'une bibliotheque de vecteurs viraux
WO2001079514A2 (fr) * 2000-04-18 2001-10-25 Syngenta Participations Ag Genes d' « acide amine » glyoxylate aminotransferase d'origine vegetale et utilisations de ceux-ci
WO2002004628A2 (fr) 2000-07-06 2002-01-17 Genvec, Inc. Procede d'identification d'un produit genique
US6830885B1 (en) 2000-08-18 2004-12-14 Phenogene Therapeutiques Inc. Nucleic acid molecule, method and kit for selecting a nucleic acid having a desired feature
DE10049587A1 (de) * 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
DE10061150A1 (de) 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10102389A1 (de) 2001-01-19 2002-08-01 Icon Genetics Ag Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen
WO2002059378A2 (fr) * 2001-01-25 2002-08-01 Virxsys Procedes et compositions servant a identifier la fonction d'un gene
US6800748B2 (en) 2001-01-25 2004-10-05 Large Scale Biology Corporation Cytoplasmic inhibition of gene expression and expression of a foreign protein in a monocot plant by a plant viral vector
JP2002238574A (ja) * 2001-02-16 2002-08-27 Japan International Research Center For Agricultural Services パパイア奇形葉モザイクウイルスの全長ゲノムｒｎａ
DE10114209A1 (de) 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
DE10115507A1 (de) 2001-03-29 2002-10-10 Icon Genetics Ag Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus
DE10121283B4 (de) 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen
DE10132780A1 (de) 2001-07-06 2003-01-16 Icon Genetics Ag Plastidäre Genexpression über autonom replizierende Vektoren
US7635798B2 (en) 2001-08-31 2009-12-22 Dow Agrosciences, Llc Nucleic acid compositions conferring altered metabolic characteristics
ATE465258T1 (de) * 2001-08-31 2010-05-15 Riken Pflanzensystem zur umfassenden genfunktionsanalyse unter verwendung einer vollängen-cdna
DE10143237A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
DE10143238A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente
CA2470426A1 (fr) 2001-12-18 2003-06-26 Bayer Bioscience N.V. Methodes ameliorees et dispositif d'administration d'arn inhibiteur a des vegetaux et applications associees
WO2003060134A1 (fr) * 2002-01-16 2003-07-24 Cropdesign N.V. Determination du mode d'action d'un herbicide
DE10212158A1 (de) 2002-03-19 2003-10-02 Metanomics Gmbh & Co Kgaa Population transgener Pflanzen, davon abgeleitetes biologisches Material, entsprechende Plasmidkollektion und Population transformierter Wirtsorganismen, sowie deren Verwendung und Verfahren zu deren Erzeugung
US20050251885A1 (en) * 2002-07-29 2005-11-10 Universite Laval Method for enhancing yield of recombinant protein production from plants
AU2003212136A1 (en) * 2003-03-07 2004-09-28 National Research Council Of Canada The determination of function of mammalian proteins by analysis of global gene expression profiles
CN102498998B (zh) * 2011-11-24 2013-09-18 湖南杂交水稻研究中心 一种水稻光温敏不育系种子的提纯保真繁殖方法
DE102013210506A1 (de) * 2013-06-06 2014-12-11 Henkel Ag & Co. Kgaa Alkoholoxidase mit Aktivität für Glycerol
EP3645635B1 (fr) * 2017-06-30 2021-08-04 Dow Global Technologies LLC Revêtement pour la réhabilitation d'aldéhyde et procédé de fabrication
AR112465A1 (es) 2017-07-31 2019-10-30 Reynolds Tobacco Co R Métodos y composiciones para la edición de genes de base viral en plantas
EP3682012A1 (fr) 2017-09-11 2020-07-22 R. J. Reynolds Tobacco Company Méthodes et compositions permettant d'augmenter l'expression de gènes d'intérêt dans une plante par co-expression avec p21

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922602A (en) * 1988-02-26 1999-07-13 Biosource Technologies, Inc. Cytoplasmic inhibition of gene expression
GB8916213D0 (en) * 1989-07-14 1989-08-31 Ici Plc Dna constructs,cells and plants derived therefrom
GB9703146D0 (en) * 1997-02-14 1997-04-02 Innes John Centre Innov Ltd Methods and means for gene silencing in transgenic plants

Also Published As

Publication number Publication date
WO1999036516A3 (fr) 2000-03-16
AU2328699A (en) 1999-08-02
EP1045899A2 (fr) 2000-10-25
AU761367B2 (en) 2003-06-05
KR20010040337A (ko) 2001-05-15
JP2002508957A (ja) 2002-03-26
WO1999036516A2 (fr) 1999-07-22

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued