CA2309835A1 - Vecteurs d'alphavirus - Google Patents

Vecteurs d'alphavirus Download PDF

Info

Publication number
CA2309835A1
CA2309835A1 CA002309835A CA2309835A CA2309835A1 CA 2309835 A1 CA2309835 A1 CA 2309835A1 CA 002309835 A CA002309835 A CA 002309835A CA 2309835 A CA2309835 A CA 2309835A CA 2309835 A1 CA2309835 A1 CA 2309835A1
Authority
CA
Canada
Prior art keywords
sequence
dna molecule
dna
vector
alphavirus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002309835A
Other languages
English (en)
Inventor
Mark Parrington
Michel H. Klein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Pasteur Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2309835A1 publication Critical patent/CA2309835A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/36011—Togaviridae
    • C12N2770/36111—Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
    • C12N2770/36141—Use of virus, viral particle or viral elements as a vector
    • C12N2770/36143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA

Abstract

L'invention concerne un vecteur d'expression d'alphavirus modifié, selon lequel au moins un site d'épissage hétérologue optimal est introduit dans le réplicon de l'alphavirus pour empêcher tout épissage aberrant de l'alphavirus, qui peut être le virus Semliki Forest, après administration du vecteur à un hôte.
CA002309835A 1997-11-14 1998-11-13 Vecteurs d'alphavirus Abandoned CA2309835A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6579397P 1997-11-14 1997-11-14
US60/065,793 1997-11-14
PCT/CA1998/001065 WO1999025859A1 (fr) 1997-11-14 1998-11-13 Vecteurs d'alphavirus

Publications (1)

Publication Number Publication Date
CA2309835A1 true CA2309835A1 (fr) 1999-05-27

Family

ID=22065156

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002309835A Abandoned CA2309835A1 (fr) 1997-11-14 1998-11-13 Vecteurs d'alphavirus

Country Status (11)

Country Link
EP (1) EP1029069B9 (fr)
JP (1) JP3835669B2 (fr)
AT (1) ATE334217T1 (fr)
AU (1) AU753729B2 (fr)
BR (1) BR9814171A (fr)
CA (1) CA2309835A1 (fr)
DE (1) DE69835369T2 (fr)
DK (1) DK1029069T3 (fr)
ES (1) ES2268797T3 (fr)
PT (1) PT1029069E (fr)
WO (1) WO1999025859A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6015686A (en) 1993-09-15 2000-01-18 Chiron Viagene, Inc. Eukaryotic layered vector initiation systems
US6451592B1 (en) 1996-04-05 2002-09-17 Chiron Corporation Recombinant alphavirus-based vectors with reduced inhibition of cellular macromolecular synthesis
US8647864B2 (en) 1999-04-14 2014-02-11 Novartis Ag Compositions and methods for generating an immune response utilizing alphavirus-based vector systems
EP1285080B1 (fr) 2000-05-31 2008-10-01 Novartis Vaccines and Diagnostics, Inc. Procede de purification de particules de replicon des alphavirus
ES2292271B1 (es) * 2004-05-20 2009-02-16 Proyecto De Biomedicina Cima, S.L. Un vector hibrido adenovirus-alfavirus para la administracion eficaz y expresion de genes terapeuticos en celulas tumorales.
CN1927403B (zh) * 2006-09-29 2010-09-29 张永亮 用于促进仔猪生长、提高仔猪免疫力的药物及制备方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE9401091D0 (sv) * 1994-03-31 1994-03-31 Bioption Ab Alphavirus cDNA vectors
AU4594996A (en) * 1994-11-30 1996-06-19 Chiron Viagene, Inc. Recombinant alphavirus vectors
US6019980A (en) * 1995-06-07 2000-02-01 Connaught Laboratories Limited Nucleic acid respiratory syncytial virus vaccines

Also Published As

Publication number Publication date
EP1029069B1 (fr) 2006-07-26
ATE334217T1 (de) 2006-08-15
JP3835669B2 (ja) 2006-10-18
PT1029069E (pt) 2006-12-29
DE69835369D1 (de) 2006-09-07
BR9814171A (pt) 2001-10-30
WO1999025859A1 (fr) 1999-05-27
AU1139199A (en) 1999-06-07
AU753729B2 (en) 2002-10-24
DE69835369T2 (de) 2007-08-23
DK1029069T3 (da) 2006-12-18
EP1029069A1 (fr) 2000-08-23
EP1029069B9 (fr) 2006-12-27
JP2002508925A (ja) 2002-03-26
ES2268797T3 (es) 2007-03-16

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued