CA2309000A1 - Systeme de transposon a base de tc1 - Google Patents

Systeme de transposon a base de tc1 Download PDF

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Publication number
CA2309000A1
CA2309000A1 CA002309000A CA2309000A CA2309000A1 CA 2309000 A1 CA2309000 A1 CA 2309000A1 CA 002309000 A CA002309000 A CA 002309000A CA 2309000 A CA2309000 A CA 2309000A CA 2309000 A1 CA2309000 A1 CA 2309000A1
Authority
CA
Canada
Prior art keywords
nucleic acid
cell
sequence
coding sequence
transposase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002309000A
Other languages
English (en)
Inventor
Perry B. Hackett
Karl J. Clark
Adam J. Dupuy
Stephen C. Ekker
David A. Largaespada
Zoltan Ivics
Zsuzsanna Izsvak
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Minnesota
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2309000A1 publication Critical patent/CA2309000A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/60—Vectors containing traps for, e.g. exons, promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des vecteurs de transposon codant pour des pièges à gènes et des pièges à régions de commande d'expression. La présente invention concerne également des vecteurs dicistroniques. Selon certains modes de réalisation, l'invention concerne, en outre, des sites d'entrée de ribosome interne.
CA002309000A 1997-11-13 1998-11-13 Systeme de transposon a base de tc1 Abandoned CA2309000A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6530397P 1997-11-13 1997-11-13
US60/065,303 1997-11-13
PCT/US1998/024348 WO1999025817A2 (fr) 1997-11-13 1998-11-13 Systeme de transposon a base d'adn permettant d'introduire un acide nucleique dans l'adn d'une cellule

Publications (1)

Publication Number Publication Date
CA2309000A1 true CA2309000A1 (fr) 1999-05-27

Family

ID=22061760

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002309000A Abandoned CA2309000A1 (fr) 1997-11-13 1998-11-13 Systeme de transposon a base de tc1

Country Status (5)

Country Link
EP (1) EP1034258A2 (fr)
JP (1) JP2001523450A (fr)
AU (1) AU1410399A (fr)
CA (1) CA2309000A1 (fr)
WO (1) WO1999025817A2 (fr)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2280997C (fr) 1997-03-11 2013-05-28 Perry B. Hackett Systeme transposon a base d'adn permettant d'introduire de l'acide nucleique dans l'adn d'une cellule
US7160682B2 (en) 1998-11-13 2007-01-09 Regents Of The University Of Minnesota Nucleic acid transfer vector for the introduction of nucleic acid into the DNA of a cell
WO2000068399A2 (fr) * 1999-05-11 2000-11-16 Regents Of The University Of Minnesota Diffusion de sequences de transposon integratreur par des vecteurs
WO2001029205A2 (fr) * 1999-10-19 2001-04-26 Minos Biosystems Limited Procede de manipulation genetique
WO2001030965A2 (fr) 1999-10-28 2001-05-03 The Board Of Trustees Of The Leland Stanford Junior University Methodes de transfert genique in vivo a l'aide d'un systeme de transposon «sleeping beauty»
EP1274838A1 (fr) 2000-01-28 2003-01-15 The Scripps Research Institute Sites d'entree internes du ribosome de synthese et procedes d'identification correspondants
AU2001265756A1 (en) * 2000-04-27 2001-11-07 Max-Delbruck-Centrum Fur Molekulare Medizin Sleeping beauty, a transposon vector with a broad host range for the genetic transfprmation in vertebrates
EP1286699A2 (fr) * 2000-05-19 2003-03-05 Regents Of The University Of Minnesota Composition pour l'introduction de composes dans des cellules
WO2003089618A2 (fr) 2002-04-22 2003-10-30 Regents Of The University Of Minnesota Systeme de transposons, et procedes d'utilisation
DE10224242A1 (de) * 2002-05-29 2003-12-11 Max Delbrueck Centrum Frog Prince, ein Transposonvektor für den Gentransfer bei Wirbeltieren
US7985739B2 (en) 2003-06-04 2011-07-26 The Board Of Trustees Of The Leland Stanford Junior University Enhanced sleeping beauty transposon system and methods for using the same
US7919583B2 (en) 2005-08-08 2011-04-05 Discovery Genomics, Inc. Integration-site directed vector systems
AU2008271523B2 (en) 2007-07-04 2015-09-10 Max-Delbruck-Centrum Fur Molekulare Medizin Hyperactive variants of the transposase protein of the transposon system Sleeping Beauty
WO2011153440A2 (fr) 2010-06-04 2011-12-08 Regents Of The University Of Minnesota Système de mutagenèse par transposons et ses méthodes d'utilisation
RU2688485C2 (ru) 2014-01-07 2019-05-21 Фундасио Привада Институт Де Медисина Предиктива И Персоналицада Дель Кансер Способы получения библиотек двухцепочечных днк и способы секвенирования для идентификации метилированных цитозинов
BR112020000406A2 (pt) * 2017-07-10 2022-09-13 Inst De Biologia Molecular Do Parana Ibmp Plataforma genética para superexpressão heteróloga associada à seleção de células altamente produtoras de proteínas
MX2020000900A (es) 2017-07-29 2021-01-08 Juno Therapeutics Inc Reactivos para expandir celulas que expresan receptores recombinantes.
KR20200099132A (ko) 2017-11-01 2020-08-21 주노 쎄러퓨티크스 인코퍼레이티드 조작된 세포의 치료적 조성물을 생성하기 위한 프로세스
WO2019090004A1 (fr) 2017-11-01 2019-05-09 Juno Therapeutics, Inc. Procédé pour la production d'une composition de lymphocytes t
MA51114A (fr) 2017-12-08 2020-10-14 Juno Therapeutics Inc Procédé de production d'une compositions de lymphocytes t modifiés
JP2022554348A (ja) 2019-11-05 2022-12-28 ジュノー セラピューティクス インコーポレイテッド 治療用ｔ細胞組成物の属性を決定する方法
EP4127184A4 (fr) * 2020-03-30 2024-04-24 Inst Zoology Cas Systèmes de transposon d'adn actif et leurs procédés d'utilisation
KR20230024283A (ko) 2020-05-13 2023-02-20 주노 쎄러퓨티크스 인코퍼레이티드 임상 반응과 관련된 특징을 식별하는 방법 및 이의 용도
WO2022204070A1 (fr) 2021-03-22 2022-09-29 Juno Therapeutics, Inc. Procédés de détermination de la puissance d'une composition de cellules thérapeutiques
WO2023122716A1 (fr) 2021-12-22 2023-06-29 Vanderbilt University Transpososomes de nouvelle génération
WO2023230548A1 (fr) 2022-05-25 2023-11-30 Celgene Corporation Procédé de prédiction d'une réponse à une thérapie par lymphocyte t
US20240000844A1 (en) 2022-05-27 2024-01-04 Kite Pharma, Inc. Non-viral delivery of cell therapy constructs

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0885307A1 (fr) * 1996-02-09 1998-12-23 Vereniging Het Nederlands Kanker Instituut Vecteurs et methodes afferentes permettant d'apporter a des cellules des acides nucleiques supplementaires s'integrant dans leur genome
CA2280997C (fr) * 1997-03-11 2013-05-28 Perry B. Hackett Systeme transposon a base d'adn permettant d'introduire de l'acide nucleique dans l'adn d'une cellule

Also Published As

Publication number Publication date
WO1999025817A9 (fr) 1999-09-10
JP2001523450A (ja) 2001-11-27
WO1999025817A2 (fr) 1999-05-27
WO1999025817A3 (fr) 1999-08-05
AU1410399A (en) 1999-06-07
EP1034258A2 (fr) 2000-09-13

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FZDE Discontinued