CA2309000A1 - Systeme de transposon a base de tc1 - Google Patents
Systeme de transposon a base de tc1 Download PDFInfo
- Publication number
- CA2309000A1 CA2309000A1 CA002309000A CA2309000A CA2309000A1 CA 2309000 A1 CA2309000 A1 CA 2309000A1 CA 002309000 A CA002309000 A CA 002309000A CA 2309000 A CA2309000 A CA 2309000A CA 2309000 A1 CA2309000 A1 CA 2309000A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- cell
- sequence
- coding sequence
- transposase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/60—Vectors containing traps for, e.g. exons, promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
- C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La présente invention concerne des vecteurs de transposon codant pour des pièges à gènes et des pièges à régions de commande d'expression. La présente invention concerne également des vecteurs dicistroniques. Selon certains modes de réalisation, l'invention concerne, en outre, des sites d'entrée de ribosome interne.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US6530397P | 1997-11-13 | 1997-11-13 | |
US60/065,303 | 1997-11-13 | ||
PCT/US1998/024348 WO1999025817A2 (fr) | 1997-11-13 | 1998-11-13 | Systeme de transposon a base d'adn permettant d'introduire un acide nucleique dans l'adn d'une cellule |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2309000A1 true CA2309000A1 (fr) | 1999-05-27 |
Family
ID=22061760
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002309000A Abandoned CA2309000A1 (fr) | 1997-11-13 | 1998-11-13 | Systeme de transposon a base de tc1 |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1034258A2 (fr) |
JP (1) | JP2001523450A (fr) |
AU (1) | AU1410399A (fr) |
CA (1) | CA2309000A1 (fr) |
WO (1) | WO1999025817A2 (fr) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6489458B2 (en) | 1997-03-11 | 2002-12-03 | Regents Of The University Of Minnesota | DNA-based transposon system for the introduction of nucleic acid into DNA of a cell |
US7160682B2 (en) | 1998-11-13 | 2007-01-09 | Regents Of The University Of Minnesota | Nucleic acid transfer vector for the introduction of nucleic acid into the DNA of a cell |
CA2373121A1 (fr) * | 1999-05-11 | 2000-11-16 | Regents Of The University Of Minnesota | Diffusion de sequences de transposon integratreur par des vecteurs |
AU1292301A (en) * | 1999-10-19 | 2001-04-30 | Minos Biosystems Limited | Method for genetic manipulation |
WO2001030965A2 (fr) | 1999-10-28 | 2001-05-03 | The Board Of Trustees Of The Leland Stanford Junior University | Methodes de transfert genique in vivo a l'aide d'un systeme de transposon «sleeping beauty» |
CA2398790A1 (fr) | 2000-01-28 | 2001-08-02 | The Scripps Research Institute | Procedes pour identifier des elements regulateurs transcriptionnels et translationnels synthetiques, et compositions correspondantes |
AU2001265756A1 (en) * | 2000-04-27 | 2001-11-07 | Max-Delbruck-Centrum Fur Molekulare Medizin | Sleeping beauty, a transposon vector with a broad host range for the genetic transfprmation in vertebrates |
US20020115216A1 (en) * | 2000-05-19 | 2002-08-22 | Steer Clifford J. | Composition for delivery of compounds to cells |
US8227432B2 (en) | 2002-04-22 | 2012-07-24 | Regents Of The University Of Minnesota | Transposon system and methods of use |
DE10224242A1 (de) * | 2002-05-29 | 2003-12-11 | Max Delbrueck Centrum | Frog Prince, ein Transposonvektor für den Gentransfer bei Wirbeltieren |
US7985739B2 (en) | 2003-06-04 | 2011-07-26 | The Board Of Trustees Of The Leland Stanford Junior University | Enhanced sleeping beauty transposon system and methods for using the same |
US7919583B2 (en) | 2005-08-08 | 2011-04-05 | Discovery Genomics, Inc. | Integration-site directed vector systems |
US9228180B2 (en) | 2007-07-04 | 2016-01-05 | Max-Delbruck-Centrum Fur Molekulare Medizin | Polypeptide variants of sleeping beauty transposase |
WO2011153440A2 (fr) | 2010-06-04 | 2011-12-08 | Regents Of The University Of Minnesota | Système de mutagenèse par transposons et ses méthodes d'utilisation |
EP3092308A1 (fr) | 2014-01-07 | 2016-11-16 | Fundacio Privada Institut de Medicina Predictiva i Personalitzada del Cancer | Procédé de génération de banques adn double brin et procédés de séquençage pour l'identification des cytosines méthylées |
WO2019010551A1 (fr) * | 2017-07-10 | 2019-01-17 | Instituto De Biologia Molecular Do Paraná - Ibmp | Plateforme génétique pour la surexpression hétérologue associée à la sélection de cellules hautement productrices de protéines |
WO2019027850A1 (fr) | 2017-07-29 | 2019-02-07 | Juno Therapeutics, Inc. | Réactifs d'expansion de cellules exprimant des récepteurs recombinants |
US20210254000A1 (en) | 2017-11-01 | 2021-08-19 | Juno Therapeutics, Inc. | Process for producing a t cell composition |
CN111542596A (zh) | 2017-11-01 | 2020-08-14 | 朱诺治疗学股份有限公司 | 产生工程化细胞的治疗性组合物的方法 |
BR112020011215A2 (pt) | 2017-12-08 | 2020-11-17 | Juno Therapeutics Inc | processo para a produção de uma composição de células t modificadas |
WO2021092097A1 (fr) | 2019-11-05 | 2021-05-14 | Juno Therapeutics, Inc. | Procédés de détermination d'attributs de compositions de cellules t thérapeutiques |
JP2023520083A (ja) * | 2020-03-30 | 2023-05-15 | 中国科学院動物研究所 | 活性dnaトランスポゾンシステム及びその使用方法 |
EP4150640A1 (fr) | 2020-05-13 | 2023-03-22 | Juno Therapeutics, Inc. | Procédés d'identification de caractéristiques associées à une réponse clinique et leurs utilisations |
US20240168012A1 (en) | 2021-03-22 | 2024-05-23 | Juno Therapeutics, Inc. | Methods of determining potency of a therapeutic cell composition |
WO2023122716A1 (fr) | 2021-12-22 | 2023-06-29 | Vanderbilt University | Transpososomes de nouvelle génération |
WO2023230548A1 (fr) | 2022-05-25 | 2023-11-30 | Celgene Corporation | Procédé de prédiction d'une réponse à une thérapie par lymphocyte t |
US20240000844A1 (en) | 2022-05-27 | 2024-01-04 | Kite Pharma, Inc. | Non-viral delivery of cell therapy constructs |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0885307A1 (fr) * | 1996-02-09 | 1998-12-23 | Vereniging Het Nederlands Kanker Instituut | Vecteurs et methodes afferentes permettant d'apporter a des cellules des acides nucleiques supplementaires s'integrant dans leur genome |
US6489458B2 (en) * | 1997-03-11 | 2002-12-03 | Regents Of The University Of Minnesota | DNA-based transposon system for the introduction of nucleic acid into DNA of a cell |
-
1998
- 1998-11-13 JP JP2000521183A patent/JP2001523450A/ja not_active Withdrawn
- 1998-11-13 AU AU14103/99A patent/AU1410399A/en not_active Abandoned
- 1998-11-13 EP EP98957974A patent/EP1034258A2/fr not_active Withdrawn
- 1998-11-13 CA CA002309000A patent/CA2309000A1/fr not_active Abandoned
- 1998-11-13 WO PCT/US1998/024348 patent/WO1999025817A2/fr not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
WO1999025817A9 (fr) | 1999-09-10 |
EP1034258A2 (fr) | 2000-09-13 |
JP2001523450A (ja) | 2001-11-27 |
WO1999025817A2 (fr) | 1999-05-27 |
AU1410399A (en) | 1999-06-07 |
WO1999025817A3 (fr) | 1999-08-05 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |