CA2304984A1 - Systeme binaire d'expression virale dans des plantes - Google Patents

Systeme binaire d'expression virale dans des plantes Download PDF

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Publication number
CA2304984A1
CA2304984A1 CA002304984A CA2304984A CA2304984A1 CA 2304984 A1 CA2304984 A1 CA 2304984A1 CA 002304984 A CA002304984 A CA 002304984A CA 2304984 A CA2304984 A CA 2304984A CA 2304984 A1 CA2304984 A1 CA 2304984A1
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Canada
Prior art keywords
replication
gene
plant
viral
protein
Prior art date
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Abandoned
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CA002304984A
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English (en)
Inventor
Narendra S. Yadav
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EIDP Inc
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Individual
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Publication date
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Publication of CA2304984A1 publication Critical patent/CA2304984A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/12011—Geminiviridae
    • C12N2750/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un système binaire d'expression virale régulée dans des plantes. Il est composé de deux éléments intégrés dans les chromosomes. Un élément est un proréplicon contenant des séquences virales à action cis nécessaires pour la réplication dont une séquence contient un gène ciblé. L'autre élément est un gène chimère de réplication à action trans comprenant un promoteur relié à la région codante pour la réplication de la protéine virale. Le proréplicon est exempt du gène de réplication essentiel pour la réplication du réplicon et, de ce fait, ne peut pas subir de réplication épisomique autonome. Cependant, l'expression régulée de la protéine de réplication à action trans dans des cellules de la plante contenant également le proréplicon déclenchera la libération d'un réplicon libre depuis le proréplicon intégré, ce qui produira sa réplication épisomique in trans, ainsi que l'expression du gène ciblé, s'il est présent, par l'intermédiaire de l'amplification génique. Ce système d'expression est utile à la fois pour la production de protéines étrangères et la mise en sommeil de gènes endogènes et de transgènes dans le tissu de la plante. L'expression spécifique au tissu est commandée par le choix du promoteur régulant la transcription du gène de réplication à action trans.
CA002304984A 1997-10-24 1998-10-23 Systeme binaire d'expression virale dans des plantes Abandoned CA2304984A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US6350497P 1997-10-24 1997-10-24
US60/063,504 1997-10-24
US10155898P 1998-09-23 1998-09-23
US60/101,558 1998-09-23
PCT/US1998/022688 WO1999022003A1 (fr) 1997-10-24 1998-10-23 Systeme binaire d'expression virale dans des plantes

Publications (1)

Publication Number Publication Date
CA2304984A1 true CA2304984A1 (fr) 1999-05-06

Family

ID=26743483

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002304984A Abandoned CA2304984A1 (fr) 1997-10-24 1998-10-23 Systeme binaire d'expression virale dans des plantes

Country Status (7)

Country Link
EP (1) EP1025234A1 (fr)
AU (1) AU731330B2 (fr)
BR (1) BR9815260A (fr)
CA (1) CA2304984A1 (fr)
HU (1) HUP0004222A3 (fr)
PL (1) PL340297A1 (fr)
WO (1) WO1999022003A1 (fr)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69941451D1 (de) * 1998-04-01 2009-11-05 Univ North Carolina State Verfahren zur unterdrückung der genexpression in pflanzen
US6759571B1 (en) 1998-04-01 2004-07-06 North Carolina State University Method of suppressing gene expression in plants
US7468475B2 (en) 2000-06-16 2008-12-23 Schmuelling Thomas Method for modifying plant morphology, biochemistry and physiology
US6512165B1 (en) * 2000-07-10 2003-01-28 Pioneer Hi-Bred International, Inc. Methods for enhancing plant transformation frequencies
ATE469231T1 (de) 2003-11-10 2010-06-15 Icon Genetics Gmbh Von rna-virus abgeleitetes pflanzenexpressionssystem
US9267143B2 (en) 2003-11-10 2016-02-23 Icon Genetics Gmbh RNA virus-derived plant expression system
EP1564295A1 (fr) 2004-01-23 2005-08-17 Icon Genetics AG Système d'expression derivé des virus à ARN
EP1616959A1 (fr) 2004-07-07 2006-01-18 Icon Genetics AG Procédé d'expression transitoire de protéines dans les plantes biologiquement sur
US9187761B2 (en) 2006-09-25 2015-11-17 Thomas Schmulling Transcriptional repressors of cytokinin signaling and their use
ATE550350T1 (de) 2009-07-10 2012-04-15 Univ Berlin Freie Rock2 und rock3, zwei neue gain-of-function- varianten der cytokininrezeptoren ahk2 und ahk3
EP2418283A1 (fr) 2010-08-07 2012-02-15 Nomad Bioscience GmbH Procédé de transfection de plantes
EP2584042A1 (fr) 2011-10-17 2013-04-24 Nomad Bioscience GmbH Production, stockage et utilisation d'enzymes dégradant la paroi cellulaire
EP2647715A1 (fr) 2012-04-03 2013-10-09 Nomad Bioscience GmbH Agrobacterium pour transfection transitoire de plantes entières
WO2014187571A1 (fr) 2013-05-23 2014-11-27 Nomad Bioscience Gmbh Procédé de production de plantes à résistance au stress abiotique
EP3097783B1 (fr) 2015-05-26 2019-11-13 Nomad Bioscience GmbH Colicine m pour le contrôle de ehec
EP3372091A1 (fr) 2017-03-07 2018-09-12 Nomad Bioscience GmbH Procédé de réduction de la contamination d'un objet par clostridium
EP3378485A1 (fr) 2017-03-24 2018-09-26 Nomad Bioscience GmbH Bactériocines pour le contrôle de salmonella enterica
JP2021514391A (ja) 2018-02-15 2021-06-10 アイコン ジェネティクス ゲーエムベーハー ノロウイルスに対する免疫応答を生成するための免疫原性組成物及びワクチン
US20220152186A1 (en) 2019-03-12 2022-05-19 Icon Genetics Gmbh Norovirus-like particles with improved stability
BR112021024197A2 (pt) 2019-06-06 2022-04-26 Nomad Bioscience Gmbh Klebicinas para o controle de klebsiella
MX2023000708A (es) 2020-07-16 2023-02-14 Nomad Bioscience Gmbh Productos para consumo oral con contenido reducido de azucar.
EP4248987A1 (fr) 2022-03-21 2023-09-27 Nomad Bioscience GmbH Bactériocines chimériques et procédé de lutte contre les pseudomonas

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3686633T2 (de) * 1985-10-25 1993-04-15 David Matthew Bisaro Pflanzenvektoren.
NL9001711A (nl) * 1989-10-03 1991-05-01 Clovis Matton N V Genetische manipulaties met recombinant dna, dat van rna virus afgeleide sequenties omvat.
CA2156720A1 (fr) * 1993-02-26 1994-09-01 Jean C. Kridl Systeme d'expression de gene a base de geminivirus
US6077992A (en) * 1997-10-24 2000-06-20 E. I. Du Pont De Nemours And Company Binary viral expression system in plants

Also Published As

Publication number Publication date
EP1025234A1 (fr) 2000-08-09
AU1122599A (en) 1999-05-17
HUP0004222A2 (en) 2001-03-28
HUP0004222A3 (en) 2002-11-28
PL340297A1 (en) 2001-01-29
BR9815260A (pt) 2000-11-21
AU731330B2 (en) 2001-03-29
WO1999022003A1 (fr) 1999-05-06

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FZDE Discontinued