CA2289566A1 - Procede de numeration de cellules viables - Google Patents
Procede de numeration de cellules viables Download PDFInfo
- Publication number
- CA2289566A1 CA2289566A1 CA002289566A CA2289566A CA2289566A1 CA 2289566 A1 CA2289566 A1 CA 2289566A1 CA 002289566 A CA002289566 A CA 002289566A CA 2289566 A CA2289566 A CA 2289566A CA 2289566 A1 CA2289566 A1 CA 2289566A1
- Authority
- CA
- Canada
- Prior art keywords
- viability
- cells
- markers
- mixture
- cfda
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 230000009467 reduction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- SOUHUMACVWVDME-UHFFFAOYSA-N safranin O Chemical compound [Cl-].C12=CC(N)=CC=C2N=C2C=CC(N)=CC2=[N+]1C1=CC=CC=C1 SOUHUMACVWVDME-UHFFFAOYSA-N 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 150000004684 trihydrates Chemical class 0.000 description 1
- OHFMKAFDZZJAEJ-UHFFFAOYSA-K trisodium;phosphate;trihydrate Chemical compound O.O.O.[Na+].[Na+].[Na+].[O-]P([O-])([O-])=O OHFMKAFDZZJAEJ-UHFFFAOYSA-K 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9706890A FR2764305B1 (fr) | 1997-06-04 | 1997-06-04 | Procede de detection et de numeration de cellules viables dans un echantillon biologique et kit pour sa mise en oeuvre |
| FR97/06890 | 1997-06-04 | ||
| PCT/FR1998/001019 WO1998055861A1 (fr) | 1997-06-04 | 1998-05-20 | Procede de numeration de cellules viables |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2289566A1 true CA2289566A1 (fr) | 1998-12-10 |
Family
ID=9507583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002289566A Abandoned CA2289566A1 (fr) | 1997-06-04 | 1998-05-20 | Procede de numeration de cellules viables |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0986752A1 (de) |
| JP (1) | JP2002505578A (de) |
| AU (1) | AU7775498A (de) |
| CA (1) | CA2289566A1 (de) |
| FR (1) | FR2764305B1 (de) |
| WO (1) | WO1998055861A1 (de) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8993259B2 (en) | 2012-05-02 | 2015-03-31 | Charles River Laboratories, Inc. | Method of viability staining with membrane permeable fluorescent dye and membrane impermeable fluorescence quencher |
| US9709500B2 (en) | 2012-05-02 | 2017-07-18 | Charles River Laboratories, Inc. | Optical method for detecting viable microorganisms in a cell sample |
| US10324036B2 (en) | 2012-05-02 | 2019-06-18 | Charles River Laboratories, Inc. | Porous planar cell capture system |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006003696A1 (ja) * | 2004-06-30 | 2006-01-12 | Fuji Electric Holdings Co., Ltd. | 生菌数の計測方法及び計測装置 |
| FR2929291B1 (fr) | 2008-04-01 | 2010-04-23 | Millipore Corp | Composition de permeabilisation cellulaire comprenant nog, hmp, chlorure de rubidium et/ou chlorure de lithium pour la detection sur membrane de cellules vivantes. |
| FR3066503B1 (fr) | 2017-05-22 | 2021-05-07 | Commissariat Energie Atomique | Procede d'analyse de microorganismes |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2707665A1 (fr) * | 1993-06-28 | 1995-01-20 | Chemunex | Procédé de mesure et d'évaluation de la vitalité des microorganismes et ses applications. |
-
1997
- 1997-06-04 FR FR9706890A patent/FR2764305B1/fr not_active Expired - Fee Related
-
1998
- 1998-05-20 EP EP98925763A patent/EP0986752A1/de not_active Withdrawn
- 1998-05-20 JP JP50170099A patent/JP2002505578A/ja active Pending
- 1998-05-20 WO PCT/FR1998/001019 patent/WO1998055861A1/fr not_active Application Discontinuation
- 1998-05-20 CA CA002289566A patent/CA2289566A1/fr not_active Abandoned
- 1998-05-20 AU AU77754/98A patent/AU7775498A/en not_active Abandoned
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8993259B2 (en) | 2012-05-02 | 2015-03-31 | Charles River Laboratories, Inc. | Method of viability staining with membrane permeable fluorescent dye and membrane impermeable fluorescence quencher |
| US8993260B2 (en) | 2012-05-02 | 2015-03-31 | Charles River Laboratories, Inc. | Fluorescence-based viability staining method using a membrane permeable flourescent dye and membrane impermeable fluorescence quencher |
| US9709500B2 (en) | 2012-05-02 | 2017-07-18 | Charles River Laboratories, Inc. | Optical method for detecting viable microorganisms in a cell sample |
| US10324036B2 (en) | 2012-05-02 | 2019-06-18 | Charles River Laboratories, Inc. | Porous planar cell capture system |
| US10976258B2 (en) | 2012-05-02 | 2021-04-13 | Charles River Laboratories, Inc. | Porous planar cell capture system and method of use |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1998055861A1 (fr) | 1998-12-10 |
| AU7775498A (en) | 1998-12-21 |
| JP2002505578A (ja) | 2002-02-19 |
| EP0986752A1 (de) | 2000-03-22 |
| FR2764305A1 (fr) | 1998-12-11 |
| FR2764305B1 (fr) | 2000-10-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |