CA2284097A1 - Shuffling of heterologous dna sequences - Google Patents
Shuffling of heterologous dna sequences Download PDFInfo
- Publication number
- CA2284097A1 CA2284097A1 CA002284097A CA2284097A CA2284097A1 CA 2284097 A1 CA2284097 A1 CA 2284097A1 CA 002284097 A CA002284097 A CA 002284097A CA 2284097 A CA2284097 A CA 2284097A CA 2284097 A1 CA2284097 A1 CA 2284097A1
- Authority
- CA
- Canada
- Prior art keywords
- sequences
- conserved region
- pcr
- primer
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 108010019653 Pwo polymerase Proteins 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK30497 | 1997-03-18 | ||
| DK0432/97 | 1997-04-17 | ||
| DK0304/97 | 1997-04-17 | ||
| DK43297 | 1997-04-17 | ||
| PCT/DK1998/000105 WO1998041623A1 (en) | 1997-03-18 | 1998-03-18 | Shuffling of heterologous dna sequences |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2284097A1 true CA2284097A1 (en) | 1998-09-24 |
Family
ID=26063733
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002284097A Abandoned CA2284097A1 (en) | 1997-03-18 | 1998-03-18 | Shuffling of heterologous dna sequences |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP1015575B1 (enExample) |
| JP (1) | JP2001516216A (enExample) |
| AT (1) | ATE468394T1 (enExample) |
| AU (1) | AU738461B2 (enExample) |
| CA (1) | CA2284097A1 (enExample) |
| DE (1) | DE69841674D1 (enExample) |
| DK (1) | DK1015575T3 (enExample) |
| IL (1) | IL131986A0 (enExample) |
| NZ (1) | NZ337910A (enExample) |
| WO (1) | WO1998041623A1 (enExample) |
Families Citing this family (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6995017B1 (en) | 1994-02-17 | 2006-02-07 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US20060257890A1 (en) | 1996-05-20 | 2006-11-16 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6936289B2 (en) | 1995-06-07 | 2005-08-30 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
| US7148054B2 (en) | 1997-01-17 | 2006-12-12 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| DE1193314T1 (de) | 1997-04-09 | 2002-10-02 | Danisco A/S, Copenhagen | Lipase und Verwendung davon zur Verbesserung von Teigen und Backwaren |
| JP3712255B2 (ja) | 1997-12-08 | 2005-11-02 | カリフォルニア・インスティチュート・オブ・テクノロジー | ポリヌクレオチドおよびポリペプチド配列を生成するための方法 |
| US7390619B1 (en) | 1998-02-11 | 2008-06-24 | Maxygen, Inc. | Optimization of immunomodulatory properties of genetic vaccines |
| WO1999057128A1 (en) | 1998-05-01 | 1999-11-11 | Maxygen, Inc. | Optimization of pest resistance genes using dna shuffling |
| US7153655B2 (en) | 1998-06-16 | 2006-12-26 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
| US6846655B1 (en) | 1998-06-29 | 2005-01-25 | Phylos, Inc. | Methods for generating highly diverse libraries |
| EP1104459A1 (en) | 1998-08-12 | 2001-06-06 | Maxygen, Inc. | Dna shuffling of monooxygenase genes for production of industrial chemicals |
| WO2000020573A2 (en) | 1998-10-07 | 2000-04-13 | Maxygen, Inc. | Dna shuffling to produce nucleic acids for mycotoxin detoxification |
| WO2000028018A1 (en) | 1998-11-10 | 2000-05-18 | Maxygen, Inc. | Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes |
| US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
| US6917882B2 (en) | 1999-01-19 | 2005-07-12 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| JP2002534966A (ja) | 1999-01-19 | 2002-10-22 | マキシジェン， インコーポレイテッド | オリゴヌクレオチド媒介核酸組換え |
| US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
| US6961664B2 (en) | 1999-01-19 | 2005-11-01 | Maxygen | Methods of populating data structures for use in evolutionary simulations |
| JP2003524394A (ja) | 1999-02-11 | 2003-08-19 | マキシジェン， インコーポレイテッド | ハイスループット質量分析法 |
| US6531316B1 (en) | 1999-03-05 | 2003-03-11 | Maxyag, Inc. | Encryption of traits using split gene sequences and engineered genetic elements |
| AU4456200A (en) * | 1999-04-13 | 2000-11-14 | Maxygen, Inc. | Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes |
| US7430477B2 (en) | 1999-10-12 | 2008-09-30 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
| US6686515B1 (en) | 1999-11-23 | 2004-02-03 | Maxygen, Inc. | Homologous recombination in plants |
| US7115712B1 (en) | 1999-12-02 | 2006-10-03 | Maxygen, Inc. | Cytokine polypeptides |
| EP1276900A2 (en) | 2000-01-11 | 2003-01-22 | Maxygen, Inc. | Integrated systems and methods for diversity generation and screening |
| US6248541B1 (en) | 2000-04-21 | 2001-06-19 | Genencor International, Inc. | Screening under nutrient limited conditions |
| US6534292B1 (en) | 2000-05-08 | 2003-03-18 | Genencor International, Inc. | Methods for forming recombined nucleic acids |
| CA2405520A1 (en) * | 2000-05-23 | 2001-11-29 | California Institute Of Technology | Gene recombination and hybrid protein development |
| JP2004513878A (ja) | 2000-06-23 | 2004-05-13 | マキシジェン， インコーポレイテッド | 新規同時刺激分子 |
| EP1297146A2 (en) | 2000-06-23 | 2003-04-02 | Maxygen, Inc. | Novel chimeric promoters |
| WO2002000852A2 (en) | 2000-06-26 | 2002-01-03 | Novozymes A/S | Lipolytic enzyme |
| US6994963B1 (en) | 2000-07-10 | 2006-02-07 | Ambion, Inc. | Methods for recombinatorial nucleic acid synthesis |
| US6858422B2 (en) | 2000-07-13 | 2005-02-22 | Codexis, Inc. | Lipase genes |
| AUPQ974900A0 (en) * | 2000-08-29 | 2000-09-21 | Macquarie Research Limited | Degenerate oligonucleotide gene-shuffling |
| US6582914B1 (en) | 2000-10-26 | 2003-06-24 | Genencor International, Inc. | Method for generating a library of oligonucleotides comprising a controlled distribution of mutations |
| US6958213B2 (en) | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
| NZ528260A (en) | 2001-05-18 | 2005-09-30 | Danisco | Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride |
| DK2295544T3 (en) | 2001-06-26 | 2016-09-26 | Novozymes As | Polypeptides with cellobiohydrolase I activity and the same coding polynucleotides |
| DE60202478T3 (de) | 2001-07-23 | 2010-01-07 | Dsm Ip Assets B.V. | Verfahren zur herstellung von polynukleotidvarianten |
| US7262012B2 (en) | 2002-05-17 | 2007-08-28 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations |
| CN101555472A (zh) | 2002-12-20 | 2009-10-14 | 诺维信公司 | 具有纤维二糖水解酶ⅱ活性的多肽及编码它的多核苷酸 |
| EP1578963B1 (en) | 2002-12-20 | 2015-02-25 | Novozymes A/S | Galactanase variants |
| MXPA05007654A (es) | 2003-01-17 | 2005-09-30 | Danisco | Metodo. |
| EP2228440B1 (en) | 2003-05-02 | 2012-08-22 | Novozymes Inc. | Variants of beta-glucosidases |
| WO2005030926A2 (en) | 2003-08-25 | 2005-04-07 | Novozymes Inc. | Variants of glycoside hydrolases |
| JP2007509603A (ja) | 2003-10-10 | 2007-04-19 | ノボザイムス アクティーゼルスカブ | プロテアーゼ変異型 |
| AU2005215852B2 (en) | 2004-02-25 | 2010-04-22 | Novozymes A/S | Fungal cell wall degrading enzyme |
| GB0405637D0 (en) | 2004-03-12 | 2004-04-21 | Danisco | Protein |
| US9045748B2 (en) | 2004-05-27 | 2015-06-02 | Novozymes, Inc. | Methods for transforming and expression screening of filamentous fungal cells with a DNA library |
| AU2005264077B2 (en) | 2004-07-16 | 2011-06-02 | Dupont Nutrition Biosciences Aps | Lipolytic enzyme uses thereof in the food industry |
| WO2016164583A2 (en) | 2015-04-07 | 2016-10-13 | Novozymes A/S | Methods for selecting enzymes having enhanced activity |
| EP3280818A2 (en) | 2015-04-07 | 2018-02-14 | Novozymes A/S | Methods for selecting enzymes having lipase activity |
| CN109312333A (zh) | 2016-04-07 | 2019-02-05 | 诺维信公司 | 选择具有蛋白酶活性的酶的方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
-
1998
- 1998-03-18 AT AT98907912T patent/ATE468394T1/de not_active IP Right Cessation
- 1998-03-18 WO PCT/DK1998/000105 patent/WO1998041623A1/en not_active Ceased
- 1998-03-18 IL IL13198698A patent/IL131986A0/xx unknown
- 1998-03-18 EP EP98907912A patent/EP1015575B1/en not_active Revoked
- 1998-03-18 DK DK98907912.4T patent/DK1015575T3/da active
- 1998-03-18 NZ NZ337910A patent/NZ337910A/en unknown
- 1998-03-18 JP JP54004498A patent/JP2001516216A/ja active Pending
- 1998-03-18 CA CA002284097A patent/CA2284097A1/en not_active Abandoned
- 1998-03-18 DE DE69841674T patent/DE69841674D1/de not_active Expired - Lifetime
- 1998-03-18 AU AU66116/98A patent/AU738461B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP1015575B1 (en) | 2010-05-19 |
| DK1015575T3 (da) | 2010-08-23 |
| WO1998041623A1 (en) | 1998-09-24 |
| DE69841674D1 (de) | 2010-07-01 |
| AU6611698A (en) | 1998-10-12 |
| NZ337910A (en) | 2001-10-26 |
| AU738461B2 (en) | 2001-09-20 |
| JP2001516216A (ja) | 2001-09-25 |
| IL131986A0 (en) | 2001-03-19 |
| EP1015575A1 (en) | 2000-07-05 |
| ATE468394T1 (de) | 2010-06-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |