DK1015575T3 - Shuffling af heterologe DNA-sekvenser - Google Patents

Shuffling af heterologe DNA-sekvenser

Info

Publication number
DK1015575T3
DK1015575T3 DK98907912.4T DK98907912T DK1015575T3 DK 1015575 T3 DK1015575 T3 DK 1015575T3 DK 98907912 T DK98907912 T DK 98907912T DK 1015575 T3 DK1015575 T3 DK 1015575T3
Authority
DK
Denmark
Prior art keywords
shuffling
conserved region
fragments
interest
identification
Prior art date
Application number
DK98907912.4T
Other languages
Danish (da)
English (en)
Inventor
Joel R Cherry
Torben Vedel Borchert
Titus Kretzschmar
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26063733&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1015575(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK1015575T3 publication Critical patent/DK1015575T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DK98907912.4T 1997-03-18 1998-03-18 Shuffling af heterologe DNA-sekvenser DK1015575T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK30497 1997-03-18
DK43297 1997-04-17
PCT/DK1998/000105 WO1998041623A1 (en) 1997-03-18 1998-03-18 Shuffling of heterologous dna sequences

Publications (1)

Publication Number Publication Date
DK1015575T3 true DK1015575T3 (da) 2010-08-23

Family

ID=26063733

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98907912.4T DK1015575T3 (da) 1997-03-18 1998-03-18 Shuffling af heterologe DNA-sekvenser

Country Status (10)

Country Link
EP (1) EP1015575B1 (enExample)
JP (1) JP2001516216A (enExample)
AT (1) ATE468394T1 (enExample)
AU (1) AU738461B2 (enExample)
CA (1) CA2284097A1 (enExample)
DE (1) DE69841674D1 (enExample)
DK (1) DK1015575T3 (enExample)
IL (1) IL131986A0 (enExample)
NZ (1) NZ337910A (enExample)
WO (1) WO1998041623A1 (enExample)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
DE1193314T1 (de) 1997-04-09 2002-10-02 Danisco A/S, Copenhagen Lipase und Verwendung davon zur Verbesserung von Teigen und Backwaren
WO1999029902A1 (en) 1997-12-08 1999-06-17 California Institute Of Technology Method for creating polynucleotide and polypeptide sequences
US7390619B1 (en) 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
CA2325567A1 (en) 1998-05-01 1999-11-11 Maxygen, Inc. Optimization of pest resistance genes using dna shuffling
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US6846655B1 (en) 1998-06-29 2005-01-25 Phylos, Inc. Methods for generating highly diverse libraries
WO2000009682A1 (en) 1998-08-12 2000-02-24 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals
WO2000020573A2 (en) 1998-10-07 2000-04-13 Maxygen, Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
EP1129184A1 (en) 1998-11-10 2001-09-05 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimation of plant phenotypes
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
WO2000042560A2 (en) 1999-01-19 2000-07-20 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
IL144657A0 (en) 1999-02-11 2002-06-30 Maxygen Inc High throughput mass spectrometry
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
AU4456200A (en) * 1999-04-13 2000-11-14 Maxygen, Inc. Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
IL150291A0 (en) 2000-01-11 2002-12-01 Maxygen Inc Integrated systems and methods for diversity generation and screening
US6248541B1 (en) 2000-04-21 2001-06-19 Genencor International, Inc. Screening under nutrient limited conditions
US6534292B1 (en) 2000-05-08 2003-03-18 Genencor International, Inc. Methods for forming recombined nucleic acids
US20020045175A1 (en) * 2000-05-23 2002-04-18 Zhen-Gang Wang Gene recombination and hybrid protein development
AU2001268716A1 (en) 2000-06-23 2002-01-08 Maxygen, Inc. Novel chimeric promoters
AU2001272978A1 (en) 2000-06-23 2002-01-08 Maxygen, Inc. Novel co-stimulatory molecules
EP2281878A1 (en) 2000-06-26 2011-02-09 Novozymes A/S Lipolytic enzyme
US6994963B1 (en) 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
AUPQ974900A0 (en) * 2000-08-29 2000-09-21 Macquarie Research Limited Degenerate oligonucleotide gene-shuffling
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
DE60220155T2 (de) 2001-05-18 2008-02-07 Danisco A/S Verfahren zur herstellung von teig mit einem enzym
DK1421224T3 (da) 2001-06-26 2013-01-21 Novozymes As Polypeptider med cellobiohydrolase I aktivitet og polynukleotider der koder for samme
EP1409667B2 (en) 2001-07-23 2009-06-10 DSM IP Assets B.V. Process for preparing variant polynucleotides
DK1504098T4 (da) 2002-05-17 2011-05-23 Alligator Bioscience Ab Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion
CN1729287A (zh) 2002-12-20 2006-02-01 诺维信公司 具有纤维二糖水解酶ⅱ活性的多肽及编码它的多核苷酸
ES2534903T3 (es) 2002-12-20 2015-04-30 Novozymes A/S Variantes de galactanasas
MXPA05007653A (es) 2003-01-17 2005-09-30 Danisco Metodo.
EP2228440B1 (en) 2003-05-02 2012-08-22 Novozymes Inc. Variants of beta-glucosidases
CN103088001A (zh) 2003-08-25 2013-05-08 诺维信股份有限公司 糖苷水解酶的变体
JP2007509603A (ja) 2003-10-10 2007-04-19 ノボザイムス アクティーゼルスカブ プロテアーゼ変異型
WO2005080559A1 (en) 2004-02-25 2005-09-01 Novozymes A/S Fungall cell wall degrading enzyme
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
DK1766040T3 (da) 2004-05-27 2017-11-13 Novozymes Inc Fremgangsmåder til transformering og ekspressions-screening af filamentøse svampeceller med et DNA-bibliotek
EP2267107A3 (en) 2004-07-16 2011-05-25 Danisco A/S Lipolytic Enzyme. Uses Thereof In The Food Industry
WO2016164583A2 (en) 2015-04-07 2016-10-13 Novozymes A/S Methods for selecting enzymes having enhanced activity
CN107864658A (zh) 2015-04-07 2018-03-30 诺维信公司 用于选择具有脂肪酶活性的酶的方法
CN109312333A (zh) 2016-04-07 2019-02-05 诺维信公司 选择具有蛋白酶活性的酶的方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination

Also Published As

Publication number Publication date
DE69841674D1 (de) 2010-07-01
EP1015575A1 (en) 2000-07-05
AU738461B2 (en) 2001-09-20
EP1015575B1 (en) 2010-05-19
ATE468394T1 (de) 2010-06-15
CA2284097A1 (en) 1998-09-24
JP2001516216A (ja) 2001-09-25
NZ337910A (en) 2001-10-26
AU6611698A (en) 1998-10-12
IL131986A0 (en) 2001-03-19
WO1998041623A1 (en) 1998-09-24

Similar Documents

Publication Publication Date Title
DK1015575T3 (da) Shuffling af heterologe DNA-sekvenser
DE69826465D1 (de) Verfahren zur in vitro evolution von proteinfunktionen
WO2000075350A3 (en) Nucleic acid sequences encoding proteins involved in fatty acid beta-oxidation and methods of use
DE60144145D1 (de) Subtilisin-variante
ATE342359T1 (de) Design von beta-faltblatt-proteinen mit spezifischen bindungseigenschaften
DE69739996D1 (de) Methoden und Zusammensetzungen zur Herstellung von Polypeptiden
NO960767L (no) Protocadherinproteiner og deres anvendelse
ATE108205T1 (de) Rekombinantverfahren zur herstellung von serinproteaseinhibitoren, sowie dns-sequenzen dazu.
ATE243753T1 (de) Mch4 und mch5, apoptotische proteasen, dafür kodierende nukleinsäure und verfahren zur verwendung
DK1117798T3 (da) Fungal transskriptionel aktivator egnet ved fremgangsmåder til produktion af polypeptider
ATE135371T1 (de) Mit akuter pankreatitis assoziiertes protein, mittel zur diagnose von akuter pankreatitis
WO1995006735A3 (en) Nucleotide sequences for novel protein tyrosine phosphatases
ATE464320T1 (de) Ntb-a, ein oberflächenmolekül impliziert in der aktivität natürlicher killerzellen (nk)
ATE291088T1 (de) Immunologisch aktive proteine von borrelia burgdorferi, dafür kodierende nukleinsäuren sowie deren verwendung in testkits und als impfstoffe
DK0672124T3 (da) Anvendelse af en DNA-sekvens, der koder for et oxalsyre-nedbrydende protein i form af et selektionsgen
ATE510911T1 (de) Prothrombin aktivierendes protein
WO2002062964A3 (en) Ap1 amine oxidase variants
DK1078072T3 (da) Nye peptidfragmenter til oprensning af proteiner
WO1999055885A3 (en) Novel serine protease capable of selective cleavage of insulin-like growth factor binding protein
ATE435914T1 (de) Induktion der blütenbildung
EP0945512A3 (en) Novel serine proteases
ATE455863T1 (de) Methoden und zusammensetzungen zur identifikation von gen-funktion
WO2005003289A3 (en) Ap1 amine oxidase variants
WO2003004595A3 (en) Materials and methods relating to protein and nucleic acid evolution