CA2278808C - Methods for cultivating cells and propagating viruses - Google Patents

Methods for cultivating cells and propagating viruses Download PDF

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Publication number
CA2278808C
CA2278808C CA002278808A CA2278808A CA2278808C CA 2278808 C CA2278808 C CA 2278808C CA 002278808 A CA002278808 A CA 002278808A CA 2278808 A CA2278808 A CA 2278808A CA 2278808 C CA2278808 C CA 2278808C
Authority
CA
Canada
Prior art keywords
cells
microcarriers
bioreactor
medium
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002278808A
Other languages
English (en)
French (fr)
Other versions
CA2278808A1 (en
Inventor
Russell G. G. Condon
Nancy V. Connelly
Andreas Frei
Edward Glowacki
Vijay Yabannavar
Serge Batandolo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Sharp and Dohme Corp
Original Assignee
Schering Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Schering Corp filed Critical Schering Corp
Priority to CA002475337A priority Critical patent/CA2475337C/en
Publication of CA2278808A1 publication Critical patent/CA2278808A1/en
Application granted granted Critical
Publication of CA2278808C publication Critical patent/CA2278808C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA002278808A 1997-01-31 1998-01-28 Methods for cultivating cells and propagating viruses Expired - Fee Related CA2278808C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002475337A CA2475337C (en) 1997-01-31 1998-01-28 Methods for cultivating cells and propagating viruses

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US79767697A 1997-01-31 1997-01-31
US79767797A 1997-01-31 1997-01-31
US08/797,676 1997-01-31
US08/797,677 1997-01-31
PCT/US1998/000911 WO1998033886A1 (en) 1997-01-31 1998-01-28 Methods for cultivating cells and propagating viruses

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002475337A Division CA2475337C (en) 1997-01-31 1998-01-28 Methods for cultivating cells and propagating viruses

Publications (2)

Publication Number Publication Date
CA2278808A1 CA2278808A1 (en) 1998-08-06
CA2278808C true CA2278808C (en) 2006-04-04

Family

ID=27121912

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002278808A Expired - Fee Related CA2278808C (en) 1997-01-31 1998-01-28 Methods for cultivating cells and propagating viruses

Country Status (10)

Country Link
EP (2) EP1731598A1 (da)
JP (2) JP2001509675A (da)
AT (1) ATE338814T1 (da)
AU (1) AU6028898A (da)
CA (1) CA2278808C (da)
DE (1) DE69835813T2 (da)
DK (1) DK0981601T3 (da)
ES (1) ES2273404T3 (da)
IL (2) IL131014A0 (da)
WO (1) WO1998033886A1 (da)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6951752B2 (en) * 2001-12-10 2005-10-04 Bexter Healthcare S.A. Method for large scale production of virus antigen
JP2003219865A (ja) * 2002-01-29 2003-08-05 Japan Tissue Engineering:Kk スフェロイドの作製方法、スフェロイド及びスフェロイド含有組成物
CN1688686A (zh) * 2002-04-08 2005-10-26 米列姆·贝尔罗吉克公司 自动化组织工程系统
AU2005214090B2 (en) 2004-02-23 2008-09-11 Crucell Holland B.V. Virus purification methods
DE102004049290A1 (de) * 2004-10-09 2006-04-20 Bayer Healthcare Ag Verfahren zur Herstellung von Virusmaterial
CN101155915B (zh) 2005-04-11 2013-12-04 克鲁塞尔荷兰公司 利用超滤进行病毒纯化
JP6303497B2 (ja) * 2013-12-27 2018-04-04 株式会社Ihi 細胞剥離回収装置及び細胞剥離回収方法及び細胞培養システム
JP2016036274A (ja) * 2014-08-06 2016-03-22 株式会社Ihi 細胞剥離方法、細胞剥離装置、及び、細胞培養システム
GB201509193D0 (en) * 2015-05-28 2015-07-15 Ge Healthcare Bio Sciences Ab Method and system for cell cultivation
EP3303549B1 (en) * 2015-05-29 2019-10-02 Mesoblast International Sàrl Methods and apparatus for separating cells from microcarriers
EP3500355B1 (en) 2016-08-21 2024-04-10 ADVA Biotechnology Ltd. Bioreactor and methods of use thereof
CA3053891C (en) * 2017-02-27 2023-05-23 I Peace, Inc. Somatic cell production system
CN108103030B (zh) * 2018-01-08 2021-04-30 兆丰华生物科技(福州)有限公司 一种微载体生物反应器培养猪圆环病毒2型的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0192705A1 (en) * 1984-08-28 1986-09-03 Massachusetts Institute Of Technology Detachment of anchorage-dependent cells from microcarriers
HRP950097A2 (en) * 1994-03-08 1997-06-30 Merck & Co Inc Hepatitis a virus culture process

Also Published As

Publication number Publication date
JP2001509675A (ja) 2001-07-24
DE69835813T2 (de) 2007-09-13
IL131014A0 (en) 2001-01-28
IL181695A0 (en) 2007-07-04
EP0981601B1 (en) 2006-09-06
CA2278808A1 (en) 1998-08-06
EP0981601A1 (en) 2000-03-01
EP1731598A1 (en) 2006-12-13
AU6028898A (en) 1998-08-25
DE69835813D1 (de) 2006-10-19
DK0981601T3 (da) 2007-01-15
ES2273404T3 (es) 2007-05-01
WO1998033886A1 (en) 1998-08-06
JP2008067720A (ja) 2008-03-27
ATE338814T1 (de) 2006-09-15

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