CA2268745A1 - Promoteurs extraits du lin permettant de manipuler l'expression genique - Google Patents
Promoteurs extraits du lin permettant de manipuler l'expression genique Download PDFInfo
- Publication number
- CA2268745A1 CA2268745A1 CA002268745A CA2268745A CA2268745A1 CA 2268745 A1 CA2268745 A1 CA 2268745A1 CA 002268745 A CA002268745 A CA 002268745A CA 2268745 A CA2268745 A CA 2268745A CA 2268745 A1 CA2268745 A1 CA 2268745A1
- Authority
- CA
- Canada
- Prior art keywords
- seq
- sequence
- plant
- flax
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0083—Miscellaneous (1.14.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
- C12N15/8226—Stem-specific, e.g. including tubers, beets
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8234—Seed-specific, e.g. embryo, endosperm
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Pregnancy & Childbirth (AREA)
- Reproductive Health (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
L'invention porte sur des séquences promoteurs obtenues à partir de lin et servant à modifier du lin ou d'autres plantes en vue de l'expression de gènes endogènes ou étrangers. Lesdits promoteurs sont des séquences SEQ ID NO 3 et SEQ ID NO 4 et proviennent de structures nouvellement découvertes de deux gènes SAD du lin, c.-à-d. les séquences SEQ ID NO 1 et SEQ ID NO 2. Les promoteurs ont été placés dans des plasmides de clonage et déposés à la "American Type Culture Collection" sous les appellations pCDC220 et pCDC214 sous les numéros respectifs de dépôt ATCC 98192 et ATCC 98193. Lesdits promoteurs peuvent être utilisés en conjonction avec des gènes pour modifier les caractéristiques du lin et d'autres plantes. L'invention inclut les gènes SAD eux-mêmes et des séquences d'ADN sensiblement homologues des SEQ ID NO 1, SEQ ID NO 2, SEQ ID NO 3 et SEQ ID NO 4, ainsi que leurs parties importantes.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US2941696P | 1996-10-31 | 1996-10-31 | |
US60/029,416 | 1996-10-31 | ||
PCT/CA1997/000812 WO1998018948A1 (fr) | 1996-10-31 | 1997-10-30 | Promoteurs extraits du lin permettant de manipuler l'expression genique |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2268745A1 true CA2268745A1 (fr) | 1998-05-07 |
Family
ID=21848902
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002268745A Abandoned CA2268745A1 (fr) | 1996-10-31 | 1997-10-30 | Promoteurs extraits du lin permettant de manipuler l'expression genique |
Country Status (4)
Country | Link |
---|---|
AU (1) | AU4858897A (fr) |
CA (1) | CA2268745A1 (fr) |
GB (1) | GB2332908B (fr) |
WO (1) | WO1998018948A1 (fr) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7642346B2 (en) * | 1999-08-27 | 2010-01-05 | Sembiosys Genetics Inc. | Flax seed specific promoters |
US6777591B1 (en) * | 1999-08-27 | 2004-08-17 | Sembiosys Genetics Inc. | Legume-like storage protein promoter isolated from flax and methods of expressing proteins in plant seeds using the promoter |
IL148270A0 (en) * | 1999-08-27 | 2002-09-12 | Sembiosys Genetics Inc | Flax seed specific promoters |
EP1925671A1 (fr) * | 2001-06-06 | 2008-05-28 | Bioriginal Food & Science Corp. | Promoteurs spécifiques à la graine lin (Linum usitatissimum L.) |
DE60228272D1 (de) | 2001-06-06 | 2008-09-25 | Bioriginal Food & Science Corp | Samen-spezifische sequenzen aus flachs (linum usitatissimum l.) |
WO2006084352A1 (fr) * | 2005-02-09 | 2006-08-17 | Bioriginal Food & Science Corp. | Nouveaux membres de la famille de la desaturase d'acides gras omega-3 et leurs utilisations |
CN102127562B (zh) | 2009-12-09 | 2013-01-30 | 安胜军 | 一种种子特异型表达载体及其构建方法与应用 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991013972A1 (fr) * | 1990-03-16 | 1991-09-19 | Calgene, Inc. | Desaturases de plantes - compositions et emplois |
CA2083259C (fr) * | 1990-05-25 | 2007-05-15 | William D. Hitz | Sequence des nucleotides du gene de la stearoyle-acp-desaturase du soja |
US5475099A (en) * | 1990-08-15 | 1995-12-12 | Calgene Inc. | Plant fatty acid synthases |
NL9002130A (nl) * | 1990-09-28 | 1992-04-16 | Stichting Tech Wetenschapp | Dna-sequentie die ten minste een deel van een gen coderend voor stearoyl-acp-desaturase omvat, alsmede toepassing van de dna-sequentie in een werkwijze voor het wijzigen van de vetzuurbiosynthese van een plant. |
DE69333034T2 (de) * | 1992-03-13 | 2004-04-01 | Agrigenetics, Inc., San Diego | Modifikation pflanzlicher Öle durch Verwendung von Desaturase |
WO1995007357A2 (fr) * | 1993-09-04 | 1995-03-16 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Promoteurs |
KR100224120B1 (ko) * | 1993-12-28 | 1999-10-15 | 마나배게이사꾸 | 지방산의불포화화효소유전자,그유전자를함유한벡터 |
-
1997
- 1997-10-30 CA CA002268745A patent/CA2268745A1/fr not_active Abandoned
- 1997-10-30 AU AU48588/97A patent/AU4858897A/en not_active Abandoned
- 1997-10-30 WO PCT/CA1997/000812 patent/WO1998018948A1/fr active Application Filing
- 1997-10-30 GB GB9908939A patent/GB2332908B/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
GB2332908A (en) | 1999-07-07 |
AU4858897A (en) | 1998-05-22 |
WO1998018948A1 (fr) | 1998-05-07 |
GB9908939D0 (en) | 1999-06-16 |
GB2332908B (en) | 2001-03-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Discontinued |