CA2436862C - Promoteurs d'elongase - Google Patents

Promoteurs d'elongase Download PDF

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Publication number
CA2436862C
CA2436862C CA2436862A CA2436862A CA2436862C CA 2436862 C CA2436862 C CA 2436862C CA 2436862 A CA2436862 A CA 2436862A CA 2436862 A CA2436862 A CA 2436862A CA 2436862 C CA2436862 C CA 2436862C
Authority
CA
Canada
Prior art keywords
nucleic acid
sequence
promoter
oil
plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2436862A
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English (en)
Other versions
CA2436862A1 (fr
Inventor
Jeroen Alexander Wilmer
Emma Jane Wallington
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biogemma UK Ltd
Original Assignee
Biogemma UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogemma UK Ltd filed Critical Biogemma UK Ltd
Publication of CA2436862A1 publication Critical patent/CA2436862A1/fr
Application granted granted Critical
Publication of CA2436862C publication Critical patent/CA2436862C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne l'utilisation de la technologie de l'ADN recombinante dans le domaine de la modification d'huiles végétales. L'invention concerne plus particulièrement l'identification de nouvelles séquences de promoteurs des gènes FAE1-1 et FAE1-2 deBrassica napus et le gène FAE1 de Arabidopsis thaliana. L'invention concerne également les cellules hôtes et les plantes transformées avec les séquences d'acides nucléiques de cette invention et les procédés permettant d'obtenir de l'huile.
CA2436862A 2000-12-22 2001-12-21 Promoteurs d'elongase Expired - Fee Related CA2436862C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0031558.0 2000-12-22
GBGB0031558.0A GB0031558D0 (en) 2000-12-22 2000-12-22 Elongase promoters
PCT/GB2001/005766 WO2002052024A2 (fr) 2000-12-22 2001-12-21 Promoteurs d'elongase

Publications (2)

Publication Number Publication Date
CA2436862A1 CA2436862A1 (fr) 2002-07-04
CA2436862C true CA2436862C (fr) 2012-06-26

Family

ID=9905825

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2436862A Expired - Fee Related CA2436862C (fr) 2000-12-22 2001-12-21 Promoteurs d'elongase

Country Status (7)

Country Link
US (1) US7169968B2 (fr)
EP (1) EP1346046B1 (fr)
AU (1) AU2002216268A1 (fr)
CA (1) CA2436862C (fr)
DE (1) DE60136956D1 (fr)
GB (1) GB0031558D0 (fr)
WO (1) WO2002052024A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8653325B2 (en) * 2005-12-15 2014-02-18 Targeted Growth, Inc. Increased seed size and seed number through transgenic over expression of revoluta protein during early embryo development
CA2612505A1 (fr) * 2006-12-28 2008-06-28 University Of Manitoba Marqueurs moleculaires specifiques du genome a haut rendement pour les genes exempts d'acide erucique de brassica napus (l.)
CA2729496C (fr) * 2008-07-01 2018-02-13 Basf Plant Science Gmbh Promoteurs de brassica napus pour l'expression de genes specifique de graines
BR112013033021A2 (pt) 2011-06-23 2017-01-31 Rho Renewables Inc "célula hospedeira e métodos para produzir composto fenólico usando policetídeo sintases aromáticas"
TWI528669B (zh) * 2014-02-19 2016-04-01 勝德國際研發股份有限公司 電源延長線及其纜線
JP7423177B2 (ja) 2014-11-14 2024-01-29 ビーエーエスエフ プラント サイエンス カンパニー ゲーエムベーハー Ｐｕｆａを含有する植物性脂質の改質

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE52800T1 (de) 1983-01-13 1990-06-15 Max Planck Gesellschaft Verfahren zum einbringen von expressionsfaehigen genen in pflanzenzellgenome und hybride ti plasmidvektoren enthaltende agrobacterium-staemme verwendbar in diesem verfahren.
ES2018274T5 (es) 1986-03-11 1996-12-16 Plant Genetic Systems Nv Celulas vegetales resistentes a los inhibidores de glutamina sintetasa, preparadas por ingenieria genetica.
NZ221259A (en) 1986-07-31 1990-05-28 Calgene Inc Seed specific transcriptional regulation
EP0265556A1 (fr) 1986-10-31 1988-05-04 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Vecteurs binaires stables pour l'agrobactérium et leur utilisation
GB8626862D0 (en) 1986-11-11 1986-12-10 Jefferson R A Gene fusion
US5589615A (en) * 1988-10-14 1996-12-31 Plant Genetic Systems N.V. Process for the production of transgenic plants with increased nutritional value via the expression of modified 2S storage albumins
GB8825402D0 (en) 1988-10-31 1988-11-30 Cambridge Advanced Tech Sulfonamide resistance genes
US5618988A (en) * 1990-03-02 1997-04-08 Amoco Corporation Enhanced carotenoid accumulation in storage organs of genetically engineered plants
DK0563191T4 (da) * 1990-12-20 2000-07-17 Du Pont Nucleotidsekvenser af sojabønne-acyl-ACP-thioesterase-gener
DK0580649T3 (da) * 1991-04-09 2001-08-27 Unilever Nv Plantepromotor inddraget i kontrol af lipidbiosyntese i frø
US5679881A (en) 1991-11-20 1997-10-21 Calgene, Inc. Nucleic acid sequences encoding a plant cytoplasmic protein involved in fatty acyl-CoA metabolism
GB9225845D0 (en) 1992-12-10 1993-02-03 Nickerson Biocem Ltd Modified plants
AU695775B2 (en) * 1993-09-04 1998-08-20 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Promoters
WO1996013582A1 (fr) 1994-10-26 1996-05-09 Dna Plant Technology Corporation Les genes fae1 et leurs utilisations
GB9502468D0 (en) 1995-02-09 1995-03-29 Gene Shears Pty Ltd DNA Sequence
GB9506684D0 (en) 1995-03-31 1995-05-24 Nickerson Biocem Ltd Control of pod dehiscence
GB9526218D0 (en) 1995-12-21 1996-02-21 Gene Shears Pty Ltd DNA Sequences
US6100450A (en) * 1997-10-22 2000-08-08 Rhone-Poulenc Agrochimie Seed specific promoters based on arabidopsis genes
WO2001011061A2 (fr) * 1999-08-04 2001-02-15 The University Of British Columbia Regulation de la transcription embryonnaire dans des plantes
DE19950589A1 (de) * 1999-10-20 2001-05-23 Gvs Ges Fuer Erwerb Und Verwer Elongasepromotoren für gewebespezifische Expression von Transgenen in Pflanzen

Also Published As

Publication number Publication date
US20040106122A1 (en) 2004-06-03
DE60136956D1 (de) 2009-01-22
GB0031558D0 (en) 2001-02-07
EP1346046A2 (fr) 2003-09-24
EP1346046B1 (fr) 2008-12-10
WO2002052024A2 (fr) 2002-07-04
WO2002052024A3 (fr) 2002-10-17
AU2002216268A1 (en) 2002-07-08
CA2436862A1 (fr) 2002-07-04
US7169968B2 (en) 2007-01-30

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Effective date: 20151221