CA2436862C - Promoteurs d'elongase - Google Patents

Promoteurs d'elongase Download PDF

Info

Publication number
CA2436862C
CA2436862C CA2436862A CA2436862A CA2436862C CA 2436862 C CA2436862 C CA 2436862C CA 2436862 A CA2436862 A CA 2436862A CA 2436862 A CA2436862 A CA 2436862A CA 2436862 C CA2436862 C CA 2436862C
Authority
CA
Canada
Prior art keywords
nucleic acid
sequence
promoter
oil
plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2436862A
Other languages
English (en)
Other versions
CA2436862A1 (fr
Inventor
Jeroen Alexander Wilmer
Emma Jane Wallington
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biogemma UK Ltd
Original Assignee
Biogemma UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogemma UK Ltd filed Critical Biogemma UK Ltd
Publication of CA2436862A1 publication Critical patent/CA2436862A1/fr
Application granted granted Critical
Publication of CA2436862C publication Critical patent/CA2436862C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne l'utilisation de la technologie de l'ADN recombinante dans le domaine de la modification d'huiles végétales. L'invention concerne plus particulièrement l'identification de nouvelles séquences de promoteurs des gènes FAE1-1 et FAE1-2 deBrassica napus et le gène FAE1 de Arabidopsis thaliana. L'invention concerne également les cellules hôtes et les plantes transformées avec les séquences d'acides nucléiques de cette invention et les procédés permettant d'obtenir de l'huile.
CA2436862A 2000-12-22 2001-12-21 Promoteurs d'elongase Expired - Fee Related CA2436862C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0031558.0A GB0031558D0 (en) 2000-12-22 2000-12-22 Elongase promoters
GB0031558.0 2000-12-22
PCT/GB2001/005766 WO2002052024A2 (fr) 2000-12-22 2001-12-21 Promoteurs d'elongase

Publications (2)

Publication Number Publication Date
CA2436862A1 CA2436862A1 (fr) 2002-07-04
CA2436862C true CA2436862C (fr) 2012-06-26

Family

ID=9905825

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2436862A Expired - Fee Related CA2436862C (fr) 2000-12-22 2001-12-21 Promoteurs d'elongase

Country Status (7)

Country Link
US (1) US7169968B2 (fr)
EP (1) EP1346046B1 (fr)
AU (1) AU2002216268A1 (fr)
CA (1) CA2436862C (fr)
DE (1) DE60136956D1 (fr)
GB (1) GB0031558D0 (fr)
WO (1) WO2002052024A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2633988A1 (fr) * 2005-12-15 2007-07-12 Targeted Growth, Inc. Taille de graines et nombre de graines accrus par la surexpression transgenique de revoluta au moment du developpement embryonnaire precoce eloppement embryonnaire precoce
US7745131B2 (en) * 2006-12-28 2010-06-29 Genyi Li High throughput genome specific molecular markers for erucic acid content genes in Brassica napus
AU2009265795B2 (en) * 2008-07-01 2014-12-11 Basf Plant Science Gmbh Promoters from Brassica napus for seed specific gene expression
AU2012272644B2 (en) 2011-06-23 2017-03-23 Rho Renewables, Inc. Recombinant production systems for aromatic molecules
TWI528669B (zh) * 2014-02-19 2016-04-01 勝德國際研發股份有限公司 電源延長線及其纜線
WO2016075313A1 (fr) * 2014-11-14 2016-05-19 Basf Plant Science Company Gmbh Matériaux et procédés pour augmenter la teneur en tocophérol dans une huile de graines

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0116718B2 (fr) 1983-01-13 1996-05-08 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Procédé pour l'introduction de gènes exprimables dans les génomes de cellules de plantes et souches d'agrobactérium contenant des vecteurs plasmidiques Ti-hybrides utilisables dans ce procédé
ATE57390T1 (de) 1986-03-11 1990-10-15 Plant Genetic Systems Nv Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen.
NZ221259A (en) 1986-07-31 1990-05-28 Calgene Inc Seed specific transcriptional regulation
EP0265556A1 (fr) 1986-10-31 1988-05-04 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Vecteurs binaires stables pour l'agrobactérium et leur utilisation
GB8626862D0 (en) 1986-11-11 1986-12-10 Jefferson R A Gene fusion
US5589615A (en) * 1988-10-14 1996-12-31 Plant Genetic Systems N.V. Process for the production of transgenic plants with increased nutritional value via the expression of modified 2S storage albumins
GB8825402D0 (en) 1988-10-31 1988-11-30 Cambridge Advanced Tech Sulfonamide resistance genes
US5618988A (en) * 1990-03-02 1997-04-08 Amoco Corporation Enhanced carotenoid accumulation in storage organs of genetically engineered plants
CA2099619C (fr) * 1990-12-20 2005-03-29 William D. Hitz Genes de la feve soja incorporant des sequences de nucleotides codant pour l'acyl-acp thioesterase
DE69231875T2 (de) * 1991-04-09 2001-10-04 Unilever Plc Pflanzen-promoter involviert in der kontrolle der lipid-biosynthese in samen
US5679881A (en) 1991-11-20 1997-10-21 Calgene, Inc. Nucleic acid sequences encoding a plant cytoplasmic protein involved in fatty acyl-CoA metabolism
GB9225845D0 (en) 1992-12-10 1993-02-03 Nickerson Biocem Ltd Modified plants
CA2169093A1 (fr) 1993-09-04 1995-03-16 Reinhard Topfer Promoteurs
CA2203754C (fr) * 1994-10-26 2010-01-12 Douglas W. James, Jr. Les genes fae1 et leurs utilisations
GB9502468D0 (en) 1995-02-09 1995-03-29 Gene Shears Pty Ltd DNA Sequence
GB9506684D0 (en) 1995-03-31 1995-05-24 Nickerson Biocem Ltd Control of pod dehiscence
GB9526218D0 (en) 1995-12-21 1996-02-21 Gene Shears Pty Ltd DNA Sequences
US6100450A (en) * 1997-10-22 2000-08-08 Rhone-Poulenc Agrochimie Seed specific promoters based on arabidopsis genes
AU6551300A (en) * 1999-08-04 2001-03-05 University Of British Columbia, The Regulation of embryonic transcription in plants
DE19950589A1 (de) * 1999-10-20 2001-05-23 Gvs Ges Fuer Erwerb Und Verwer Elongasepromotoren für gewebespezifische Expression von Transgenen in Pflanzen

Also Published As

Publication number Publication date
DE60136956D1 (de) 2009-01-22
GB0031558D0 (en) 2001-02-07
AU2002216268A1 (en) 2002-07-08
CA2436862A1 (fr) 2002-07-04
EP1346046A2 (fr) 2003-09-24
EP1346046B1 (fr) 2008-12-10
WO2002052024A2 (fr) 2002-07-04
US7169968B2 (en) 2007-01-30
WO2002052024A3 (fr) 2002-10-17
US20040106122A1 (en) 2004-06-03

Similar Documents

Publication Publication Date Title
US8030539B2 (en) Method for the stable expression of nucleic acids in transgenic plants, controlled by a parsley-ubiquitin promoter
EP2234474B1 (fr) Gènes de diacylglycérol acyltransférase 2 et protéines qu'ils codent, issus d'algues
US7193134B2 (en) Flax (Linum usitatissimum L.) fatty acid desaturase
EP1618198A1 (fr) Chalcone synthase, dihydroflavonol 4-reductase et leucoanthocyanidine reductase a partir du trefle, de fromental medicinal ou de fetuque
WO2013096562A1 (fr) Utilisation du promoteur de saccharose synthase de soja pour augmenter la teneur en lipides des graines de plantes
AU2005285015B2 (en) Promoter molecules for use in plants
CA2744379C (fr) Cassettes d'expression pour une expression specifique aux graines dans les plantes
WO2001011061A2 (fr) Regulation de la transcription embryonnaire dans des plantes
CA2436862C (fr) Promoteurs d'elongase
AU2001287627B2 (en) Method for influencing the content of sinapine in transgenic plant cells and plants
AU680551B2 (en) Glycerin-3-phosphate-dehydrogenase (GPDH)
US8148602B2 (en) Diacylglycerol acyltransferases from flax
WO1998018948A1 (fr) Promoteurs extraits du lin permettant de manipuler l'expression genique
US6784342B1 (en) Regulation of embryonic transcription in plants
US20090036695A1 (en) Oil Biosynthesis
US7179960B2 (en) Seed-associated promoter sequences
Du et al. Fatty acid elongase 1 (FAE1) promoter as a candidate for genetic engineering of fatty acids to improve seed oil composition
WO2001007586A2 (fr) Enzyme biosynthetique d'acides gras a chaines longues de plantes
CN116768991A (zh) 与油脂代谢调控相关的大豆四跨膜区蛋白GmTET270及其编码基因与应用
AU2002334151A1 (en) Production of oil with higher erucic acid content
AU2008201181A1 (en) Production of oil with higher erucic acid content
WO2011154385A1 (fr) Promoteurs de plantes et leurs utilisations

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20151221