CA2211666A1 - Procede de detection de variations dans des sequences d'acide nucleique - Google Patents
Procede de detection de variations dans des sequences d'acide nucleiqueInfo
- Publication number
- CA2211666A1 CA2211666A1 CA002211666A CA2211666A CA2211666A1 CA 2211666 A1 CA2211666 A1 CA 2211666A1 CA 002211666 A CA002211666 A CA 002211666A CA 2211666 A CA2211666 A CA 2211666A CA 2211666 A1 CA2211666 A1 CA 2211666A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- mismatch
- sequence
- dna
- solid support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention se rapporte à un procédé d'analyse d'échantillons contenant de l'acide nucléique visant à identifier des variations de séquences par rapport à des séquences normales d'acide nucléique. Ce procédé consiste à obtenir une séquence d'acide nucléique normale monocaténaire immobilisée sur un support solide, à hybrider un brin d'acide nucléique dérivé d'un échantillon sur le brin immobilisé, à soumettre le complexe d'acide nucléique obtenu à (i) un clivage induit par un mésappariement ou (ii) à des réactions d'allongement terminées par un mésappariement, et à détecter un éventuel clivage ou allongement-terminaison par mesure optique sur le support solide.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9500341A SE9500341D0 (sv) | 1995-01-30 | 1995-01-30 | Method for detecting DNA sequence variations |
SE9500341-4 | 1995-01-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2211666A1 true CA2211666A1 (fr) | 1996-08-08 |
Family
ID=20397030
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002211666A Abandoned CA2211666A1 (fr) | 1995-01-30 | 1996-01-30 | Procede de detection de variations dans des sequences d'acide nucleique |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0871766A1 (fr) |
JP (1) | JPH10513055A (fr) |
CA (1) | CA2211666A1 (fr) |
SE (1) | SE9500341D0 (fr) |
WO (1) | WO1996023903A1 (fr) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5770370A (en) * | 1996-06-14 | 1998-06-23 | David Sarnoff Research Center, Inc. | Nuclease protection assays |
EP0843019A3 (fr) * | 1996-11-08 | 2002-10-09 | Kyowa Medex Co., Ltd. | Méthode pour la détermination d'une séquence d'acide nucléique spécifique, et un réactif correspondant |
WO1998032849A2 (fr) * | 1997-01-29 | 1998-07-30 | Institut Pasteur | Polynucleotide associe au syndrome bo ou bor, applications diagnostiques et therapeutiques correspondantes de ce polypeptide |
DE1051515T1 (de) * | 1998-01-27 | 2001-04-19 | Cytocell Ltd., Banbury | Modifizierte nuklein-säure sonden und dessen verwendungen |
US7166432B2 (en) | 1999-03-12 | 2007-01-23 | Integragen | Compositions and methods for genetic analysis |
DE19911130A1 (de) * | 1999-03-12 | 2000-09-21 | Hager Joerg | Verfahren zur Identifikation chromosomaler Regionen und Gene |
US20030039964A1 (en) * | 2000-05-09 | 2003-02-27 | Giffard Philip Morrison | Method |
WO2003070977A2 (fr) * | 2002-02-21 | 2003-08-28 | Nanogen Recognomics Gmbh | Procede permettant de detecter des polymorphismes a nucleotide unique |
JPWO2004061100A1 (ja) * | 2002-12-10 | 2006-05-11 | オリンパス株式会社 | 核酸の変異解析方法および遺伝子の発現解析方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU3919293A (en) * | 1992-03-27 | 1993-11-08 | University Of Maryland At Baltimore | Detection of gene mutations with mismatch repair enzymes |
WO1993022457A1 (fr) * | 1992-04-24 | 1993-11-11 | Massachusetts Institute Of Technology | Criblage servant a detecter une variation genetique |
-
1995
- 1995-01-30 SE SE9500341A patent/SE9500341D0/xx unknown
-
1996
- 1996-01-30 JP JP8523465A patent/JPH10513055A/ja active Pending
- 1996-01-30 WO PCT/SE1996/000095 patent/WO1996023903A1/fr not_active Application Discontinuation
- 1996-01-30 EP EP96902038A patent/EP0871766A1/fr not_active Withdrawn
- 1996-01-30 CA CA002211666A patent/CA2211666A1/fr not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO1996023903A1 (fr) | 1996-08-08 |
SE9500341D0 (sv) | 1995-01-30 |
EP0871766A1 (fr) | 1998-10-21 |
JPH10513055A (ja) | 1998-12-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Dead |