CA2155677A1 - Methods for the diagnosis of diabetes and prediabetic conditions - Google Patents
Methods for the diagnosis of diabetes and prediabetic conditionsInfo
- Publication number
- CA2155677A1 CA2155677A1 CA002155677A CA2155677A CA2155677A1 CA 2155677 A1 CA2155677 A1 CA 2155677A1 CA 002155677 A CA002155677 A CA 002155677A CA 2155677 A CA2155677 A CA 2155677A CA 2155677 A1 CA2155677 A1 CA 2155677A1
- Authority
- CA
- Canada
- Prior art keywords
- gad
- antigen
- iddm
- serum
- isoforms
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 45
- 206010012601 diabetes mellitus Diseases 0.000 title claims abstract description 22
- 206010018429 Glucose tolerance impaired Diseases 0.000 title claims abstract description 10
- 238000003745 diagnosis Methods 0.000 title description 5
- 108091022930 Glutamate decarboxylase Proteins 0.000 claims abstract description 290
- 102000008214 Glutamate decarboxylase Human genes 0.000 claims abstract description 290
- 210000002966 serum Anatomy 0.000 claims abstract description 80
- 239000000427 antigen Substances 0.000 claims abstract description 49
- 102000036639 antigens Human genes 0.000 claims abstract description 49
- 108091007433 antigens Proteins 0.000 claims abstract description 49
- 238000002360 preparation method Methods 0.000 claims abstract description 44
- 239000000539 dimer Substances 0.000 claims abstract description 28
- 102000001708 Protein Isoforms Human genes 0.000 claims abstract description 14
- 108010029485 Protein Isoforms Proteins 0.000 claims abstract description 14
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- 238000001514 detection method Methods 0.000 claims description 7
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- 230000000717 retained effect Effects 0.000 claims description 3
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 106
- 238000003556 assay Methods 0.000 description 32
- 230000009257 reactivity Effects 0.000 description 29
- 241000282320 Panthera leo Species 0.000 description 23
- 241001237728 Precis Species 0.000 description 17
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 16
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- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 5
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- 238000001262 western blot Methods 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 102000016227 Protein disulphide isomerases Human genes 0.000 description 3
- 108050004742 Protein disulphide isomerases Proteins 0.000 description 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000000599 auto-anti-genic effect Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
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- 239000003638 chemical reducing agent Substances 0.000 description 3
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- 210000001995 reticulocyte Anatomy 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 102100035902 Glutamate decarboxylase 1 Human genes 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- YFONKFDEZLYQDH-OPQQBVKSSA-N N-[(1R,2S)-2,6-dimethyindan-1-yl]-6-[(1R)-1-fluoroethyl]-1,3,5-triazine-2,4-diamine Chemical compound C[C@@H](F)C1=NC(N)=NC(N[C@H]2C3=CC(C)=CC=C3C[C@@H]2C)=N1 YFONKFDEZLYQDH-OPQQBVKSSA-N 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
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- 210000004027 cell Anatomy 0.000 description 2
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- 238000001502 gel electrophoresis Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 238000003119 immunoblot Methods 0.000 description 2
- 230000000984 immunochemical effect Effects 0.000 description 2
- 108091005979 iodinated proteins Proteins 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- QVRVXSZKCXFBTE-UHFFFAOYSA-N n-[4-(6,7-dimethoxy-3,4-dihydro-1h-isoquinolin-2-yl)butyl]-2-(2-fluoroethoxy)-5-methylbenzamide Chemical compound C1C=2C=C(OC)C(OC)=CC=2CCN1CCCCNC(=O)C1=CC(C)=CC=C1OCCF QVRVXSZKCXFBTE-UHFFFAOYSA-N 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 235000004252 protein component Nutrition 0.000 description 2
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- 238000004448 titration Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 208000035408 type 1 diabetes mellitus 1 Diseases 0.000 description 2
- 108091006112 ATPases Proteins 0.000 description 1
- 235000003625 Acrocomia mexicana Nutrition 0.000 description 1
- 244000202285 Acrocomia mexicana Species 0.000 description 1
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 1
- 101000873533 Arabidopsis thaliana Glutamate decarboxylase 1 Proteins 0.000 description 1
- 102100035875 C-C chemokine receptor type 5 Human genes 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N EtOH Substances CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 101150014889 Gad1 gene Proteins 0.000 description 1
- 208000034826 Genetic Predisposition to Disease Diseases 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 101710178376 Heat shock 70 kDa protein Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000946926 Homo sapiens C-C chemokine receptor type 5 Proteins 0.000 description 1
- 101000873546 Homo sapiens Glutamate decarboxylase 1 Proteins 0.000 description 1
- 108010036012 Iodide peroxidase Proteins 0.000 description 1
- 241001495016 Loriidae Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 101100508520 Mus musculus Nfkbiz gene Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 208000001280 Prediabetic State Diseases 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 206010072148 Stiff-Person syndrome Diseases 0.000 description 1
- 102000014267 Thyroid peroxidases Human genes 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000001261 affinity purification Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- VREFGVBLTWBCJP-UHFFFAOYSA-N alprazolam Chemical compound C12=CC(Cl)=CC=C2N2C(C)=NN=C2CN=C1C1=CC=CC=C1 VREFGVBLTWBCJP-UHFFFAOYSA-N 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
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- 238000012512 characterization method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000002967 competitive immunoassay Methods 0.000 description 1
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- 230000007717 exclusion Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000001024 immunotherapeutic effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
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- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
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- 230000003389 potentiating effect Effects 0.000 description 1
- 201000009104 prediabetes syndrome Diseases 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
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- 102000004196 processed proteins & peptides Human genes 0.000 description 1
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- 201000007846 type 1 diabetes mellitus 22 Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/04—Endocrine or metabolic disorders
- G01N2800/042—Disorders of carbohydrate metabolism, e.g. diabetes, glucose metabolism
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Rehabilitation Therapy (AREA)
- General Engineering & Computer Science (AREA)
- Rheumatology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPL716893 | 1993-02-09 | ||
| AUPL7168 | 1993-02-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2155677A1 true CA2155677A1 (en) | 1994-08-18 |
Family
ID=3776693
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002155677A Abandoned CA2155677A1 (en) | 1993-02-09 | 1994-02-09 | Methods for the diagnosis of diabetes and prediabetic conditions |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US5770381A (enExample) |
| EP (1) | EP0686262B1 (enExample) |
| JP (1) | JP3510889B2 (enExample) |
| AT (1) | ATE229650T1 (enExample) |
| CA (1) | CA2155677A1 (enExample) |
| DE (1) | DE69431874D1 (enExample) |
| IL (1) | IL108587A (enExample) |
| NZ (1) | NZ261541A (enExample) |
| SG (1) | SG45161A1 (enExample) |
| TW (1) | TW305936B (enExample) |
| WO (1) | WO1994018568A1 (enExample) |
| ZA (1) | ZA94845B (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5821334A (en) * | 1996-01-26 | 1998-10-13 | Vanderbilt University | Insulin-dependent diabetes mellitus-specific chimeric polypeptides |
| FR2759701B1 (fr) * | 1997-02-19 | 1999-06-04 | Inst Nat Sante Rech Med | Utilisation des proteines ulip dans le diagnostic et la therapie des cancers et des syndromes neurologiques paraneoplasiques |
| AU3958999A (en) * | 1999-06-01 | 2000-12-18 | Kyokuto Pharmaceutical Industrial Co., Ltd. | Method for assaying anti-gad antibody and kit |
| ES2684976T3 (es) | 2012-04-13 | 2018-10-05 | Diabetomics, Inc. | Biomarcadores maternos para diabetes gestacional |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6682906B1 (en) * | 1990-09-21 | 2004-01-27 | The Regents Of The University Of California | Cloned glutamic acid decarboxylase |
| DE69233507T2 (de) * | 1991-02-22 | 2009-09-10 | The Walter And Eliza Hall Institute Of Medical Research, Parkville | Hbgad und higad polypeptide und nukleinsäuren und deren verwendung zur diagnose und behandlung von gad autoantigen assoziierten krankheiten |
| WO1992019972A1 (en) * | 1991-05-03 | 1992-11-12 | Novo Nordisk A/S | Islet cell autoantigen (gad) for the detection of preclinical diabetes |
-
1994
- 1994-02-08 TW TW083101065A patent/TW305936B/zh active
- 1994-02-08 ZA ZA94845A patent/ZA94845B/xx unknown
- 1994-02-08 IL IL108587A patent/IL108587A/en not_active IP Right Cessation
- 1994-02-09 WO PCT/AU1994/000056 patent/WO1994018568A1/en not_active Ceased
- 1994-02-09 US US08/495,584 patent/US5770381A/en not_active Expired - Fee Related
- 1994-02-09 EP EP94906796A patent/EP0686262B1/en not_active Expired - Lifetime
- 1994-02-09 CA CA002155677A patent/CA2155677A1/en not_active Abandoned
- 1994-02-09 JP JP51744894A patent/JP3510889B2/ja not_active Expired - Fee Related
- 1994-02-09 SG SG1996000807A patent/SG45161A1/en unknown
- 1994-02-09 AT AT94906796T patent/ATE229650T1/de not_active IP Right Cessation
- 1994-02-09 NZ NZ261541A patent/NZ261541A/en unknown
- 1994-02-09 DE DE69431874T patent/DE69431874D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP3510889B2 (ja) | 2004-03-29 |
| IL108587A (en) | 1998-01-04 |
| US5770381A (en) | 1998-06-23 |
| EP0686262A1 (en) | 1995-12-13 |
| TW305936B (enExample) | 1997-05-21 |
| NZ261541A (en) | 1996-09-25 |
| WO1994018568A1 (en) | 1994-08-18 |
| IL108587A0 (en) | 1994-05-30 |
| SG45161A1 (en) | 1998-01-16 |
| ZA94845B (en) | 1994-11-11 |
| EP0686262B1 (en) | 2002-12-11 |
| EP0686262A4 (en) | 1997-06-04 |
| ATE229650T1 (de) | 2002-12-15 |
| JPH08506417A (ja) | 1996-07-09 |
| DE69431874D1 (de) | 2003-01-23 |
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| Publication | Publication Date | Title |
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| Pietropaolo et al. | Combined analysis of GAD65 and ICA512 (IA-2) autoantibodies in organ and non-organ-specific autoimmune diseases confers high specificity for insulin-dependent diabetes mellitus | |
| Kubicka-Muranyi et al. | Murine systemic autoimmune disease induced by mercuric chloride: T helper cells reacting to self proteins | |
| Masuda et al. | Autoantibodies to IA-2 in insulin-dependent diabetes mellitus: measurements with a new immunoprecipitation assay | |
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