CA2140278C - Enriching and identyfing fetal cells in maternal blood for in situ hybridization - Google Patents

Enriching and identyfing fetal cells in maternal blood for in situ hybridization Download PDF

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Publication number
CA2140278C
CA2140278C CA002140278A CA2140278A CA2140278C CA 2140278 C CA2140278 C CA 2140278C CA 002140278 A CA002140278 A CA 002140278A CA 2140278 A CA2140278 A CA 2140278A CA 2140278 C CA2140278 C CA 2140278C
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CA
Canada
Prior art keywords
cells
fetal
label
individual
ribonucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002140278A
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English (en)
French (fr)
Other versions
CA2140278A1 (en
Inventor
Morteza Asgari
Nagindra Prashad
Michael L. Cubbage
Shyh-Chen Ju
Mark Blick
Joel Bresser
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Abbott Molecular Inc
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Vysis Inc
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Publication of CA2140278A1 publication Critical patent/CA2140278A1/en
Application granted granted Critical
Publication of CA2140278C publication Critical patent/CA2140278C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/36—Gynecology or obstetrics
    • G01N2800/368—Pregnancy complicated by disease or abnormalities of pregnancy, e.g. preeclampsia, preterm labour

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002140278A 1992-07-17 1993-07-19 Enriching and identyfing fetal cells in maternal blood for in situ hybridization Expired - Lifetime CA2140278C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US91596592A 1992-07-17 1992-07-17
US915,965 1992-07-17
PCT/US1993/006828 WO1994002646A1 (en) 1992-07-17 1993-07-19 Enriching and identyfying fetal cells in maternal blood for in situ hybridization

Publications (2)

Publication Number Publication Date
CA2140278A1 CA2140278A1 (en) 1994-02-03
CA2140278C true CA2140278C (en) 2009-03-17

Family

ID=25436486

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002140278A Expired - Lifetime CA2140278C (en) 1992-07-17 1993-07-19 Enriching and identyfing fetal cells in maternal blood for in situ hybridization

Country Status (6)

Country Link
EP (1) EP0662152A1 (pt)
JP (1) JPH07509136A (pt)
AU (1) AU4685593A (pt)
BR (1) BR9306867A (pt)
CA (1) CA2140278C (pt)
WO (1) WO1994002646A1 (pt)

Families Citing this family (45)

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US5457024A (en) * 1993-01-22 1995-10-10 Aprogenex, Inc. Isolation of fetal erythrocytes
US6136540A (en) * 1994-10-03 2000-10-24 Ikonisys Inc. Automated fluorescence in situ hybridization detection of genetic abnormalities
US5501954A (en) * 1994-06-13 1996-03-26 Genzyme Corporation Method of detecting cellular material
GB9422504D0 (en) 1994-11-08 1995-01-04 Robertson Patricia M B Blood testing
US5750339A (en) * 1994-11-30 1998-05-12 Thomas Jefferson University Methods for identifying fetal cells
US5998596A (en) * 1995-04-04 1999-12-07 The United States Of America As Represented By The Department Of Health And Human Services Inhibition of protein kinase activity by aptameric action of oligonucleotides
JP2000508171A (ja) * 1996-04-05 2000-07-04 ザ・ジョンズ・ホプキンス・ユニバーシティ・スクール・オブ・メディシン 希少細胞の富化方法
FR2751989B1 (fr) * 1996-08-01 1998-10-30 Soc D Rech Et De Dev En Activa Methode d'identification de cellules eucaryotes par fixation desdites cellules sur un support et support pour mettre en oeuvre ladite methode
US5731156A (en) * 1996-10-21 1998-03-24 Applied Imaging, Inc. Use of anti-embryonic hemoglobin antibodies to identify fetal cells
WO1998037919A1 (en) * 1997-02-28 1998-09-03 University Of Iowa Research Foundation USE OF NUCLEIC ACIDS CONTAINING UNMETHYLATED CpG DINUCLEOTIDE IN THE TREATMENT OF LPS-ASSOCIATED DISORDERS
GB9807045D0 (en) * 1998-04-01 1998-06-03 Rudi Knut Nucleic acid detection method
US7901887B2 (en) 1998-05-09 2011-03-08 Ikonisys, Inc. Automated cancer diagnostic methods using fish
KR100691043B1 (ko) 1998-05-09 2007-03-09 아이코니시스 인코포레이티드 컴퓨터 제어된 태아 세포 포함 희귀 세포 진단 방법 및 장치
GB2361996B (en) * 1998-10-29 2004-05-12 Cell Works Inc Multiple marker characterization of single cells
US6221596B1 (en) * 1999-05-17 2001-04-24 Motobit Ltd. System and method for identifying and isolating rare cells from a mixed population of cells
AU2922701A (en) * 1999-11-04 2001-05-14 Arcturus Engineering, Inc. Automated laser capture microdissection
AU1918100A (en) 1999-11-18 2001-05-30 Ikonisys Inc. Method and apparatus for computer controlled cell based diagnosis
US20060073509A1 (en) * 1999-11-18 2006-04-06 Michael Kilpatrick Method for detecting and quantitating multiple subcellular components
US6692965B1 (en) * 1999-11-23 2004-02-17 Chromocell Corporation Isolation of living cells and preparation of cell lines based on detection and quantification of preselected cellular ribonucleic acid sequences
GB0009784D0 (en) 2000-04-20 2000-06-07 Simeg Limited Methods for clinical diagnosis
US7083924B2 (en) 2000-07-10 2006-08-01 Btg International Limited Diagnostic method for the identification of foetal DNA in a material sample
GB0016742D0 (en) * 2000-07-10 2000-08-30 Simeg Limited Diagnostic method
US6956695B2 (en) 2001-03-19 2005-10-18 Ikonisys, Inc. System and method for increasing the contrast of an image produced by an epifluorescence microscope
US10156501B2 (en) 2001-11-05 2018-12-18 Life Technologies Corporation Automated microdissection instrument for determining a location of a laser beam projection on a worksurface area
EP1413874A1 (en) 2002-10-16 2004-04-28 Streck Laboratories, Inc. Method and device for collecting and preserving cells for analysis
CN104109712A (zh) 2004-02-18 2014-10-22 克罗莫塞尔公司 使用信号探针的方法和材料
US20090305236A1 (en) * 2005-05-11 2009-12-10 Genetic Technologies Limited Methods of enriching fetal cells
JP2010500573A (ja) 2006-08-04 2010-01-07 イコニシス インコーポレーテッド 顕微鏡システムのための画像処理方法
JP2009072198A (ja) * 2008-10-24 2009-04-09 Chromocell Corp ｍＲＮＡ結合プローブを用いる生存細胞の選択および単離
US11634747B2 (en) 2009-01-21 2023-04-25 Streck Llc Preservation of fetal nucleic acids in maternal plasma
EP2389455A4 (en) * 2009-01-26 2012-12-05 Verinata Health Inc METHODS AND COMPOSITIONS FOR IDENTIFYING A FETAL CELL
NO2398912T3 (pt) 2009-02-18 2018-02-10
SI2638176T1 (en) 2010-11-09 2018-01-31 Arcedi Biotech Aps Enrichment and determination of fetal cells in the mother's blood and ligands for such use
EP2704740B1 (en) 2011-05-04 2016-10-05 Streck, Inc. Inactivated swine flu virus and methods of preparing it
WO2014201138A1 (en) * 2013-06-11 2014-12-18 Stelling James R Method for detection of fetal abnormalities
US10091984B2 (en) 2013-07-24 2018-10-09 Streck, Inc. Compositions and methods for stabilizing circulating tumor cells
US9709556B2 (en) 2013-12-11 2017-07-18 Analiza, Inc. Devices and methods for determining and/or isolating circulating cancer cells
US20150160246A1 (en) 2013-12-11 2015-06-11 Analiza, Inc. Devices and methods for determining and/or isolating cells such as circulating cancer or fetal cells
US11168351B2 (en) 2015-03-05 2021-11-09 Streck, Inc. Stabilization of nucleic acids in urine
DK3289098T3 (da) * 2015-04-30 2024-04-02 Sakura Finetek Usa Inc Fremgangsmåder til at reducere fordampning ved forhøjet temperatur
US20170145475A1 (en) 2015-11-20 2017-05-25 Streck, Inc. Single spin process for blood plasma separation and plasma composition including preservative
CA2983930C (en) * 2016-04-15 2023-05-02 Sakura Finetek U.S.A., Inc. Methods to reduce evaporation during elevated temperature
EP3491381A1 (en) 2016-07-29 2019-06-05 Streck, Inc. Suspension composition for hematology analysis control
CN112980779B (zh) * 2021-05-20 2021-08-24 广州凯普医药科技有限公司 一种从孕妇宫颈脱落细胞中分离胎盘滋养层细胞的方法
WO2023178295A1 (en) 2022-03-18 2023-09-21 Ludwig Institute For Cancer Research Ltd Methods and systems for analyzing chromatins

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4727021A (en) * 1984-06-01 1988-02-23 Sloan-Kettering Institute For Cancer Research Human monoclonal antibodies to cytokeratin
DE68928080T2 (de) * 1988-08-31 1998-01-15 Aprogenex Inc Einstufiges in situ-hybridierungstestverfahren
JPH04505556A (ja) * 1989-03-07 1992-10-01 シンジェン，インコーポレイテッド 細胞の検出または分離のための浮遊状態におけるインシトゥハイブリッド形成法
US5153117A (en) * 1990-03-27 1992-10-06 Genetype A.G. Fetal cell recovery method

Also Published As

Publication number Publication date
JPH07509136A (ja) 1995-10-12
BR9306867A (pt) 1998-12-08
WO1994002646A1 (en) 1994-02-03
EP0662152A1 (en) 1995-07-12
CA2140278A1 (en) 1994-02-03
AU4685593A (en) 1994-02-14

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