CA2078703C - Process for detecting specific mrna and dna in cells - Google Patents
Process for detecting specific mrna and dna in cells Download PDFInfo
- Publication number
- CA2078703C CA2078703C CA002078703A CA2078703A CA2078703C CA 2078703 C CA2078703 C CA 2078703C CA 002078703 A CA002078703 A CA 002078703A CA 2078703 A CA2078703 A CA 2078703A CA 2078703 C CA2078703 C CA 2078703C
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- Prior art keywords
- cells
- sequences
- sequence
- cdna
- lysate
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US76446291A | 1991-09-23 | 1991-09-23 | |
| US764,462 | 1991-09-23 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2078703A1 CA2078703A1 (en) | 1993-03-24 |
| CA2078703C true CA2078703C (en) | 2000-10-17 |
Family
ID=25070802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002078703A Expired - Fee Related CA2078703C (en) | 1991-09-23 | 1992-09-21 | Process for detecting specific mrna and dna in cells |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5643730A (en:Method) |
| EP (1) | EP0534640B1 (en:Method) |
| JP (1) | JP2703156B2 (en:Method) |
| AT (1) | ATE143700T1 (en:Method) |
| CA (1) | CA2078703C (en:Method) |
| DE (1) | DE69214243T2 (en:Method) |
| DK (1) | DK0534640T3 (en:Method) |
| ES (1) | ES2092056T3 (en:Method) |
| FI (1) | FI103413B1 (en:Method) |
| GR (1) | GR3021721T3 (en:Method) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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- 1992-09-09 DK DK92308190.5T patent/DK0534640T3/da active
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- 1992-09-09 DE DE69214243T patent/DE69214243T2/de not_active Expired - Fee Related
- 1992-09-09 EP EP92308190A patent/EP0534640B1/en not_active Expired - Lifetime
- 1992-09-09 ES ES92308190T patent/ES2092056T3/es not_active Expired - Lifetime
- 1992-09-21 JP JP4251156A patent/JP2703156B2/ja not_active Expired - Fee Related
- 1992-09-21 CA CA002078703A patent/CA2078703C/en not_active Expired - Fee Related
- 1992-09-22 FI FI924242A patent/FI103413B1/fi active
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1995
- 1995-03-14 US US08/403,555 patent/US5643730A/en not_active Expired - Lifetime
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1996
- 1996-11-20 GR GR960403116T patent/GR3021721T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2078703A1 (en) | 1993-03-24 |
| FI103413B (fi) | 1999-06-30 |
| EP0534640B1 (en) | 1996-10-02 |
| GR3021721T3 (en) | 1997-02-28 |
| EP0534640A1 (en) | 1993-03-31 |
| DE69214243D1 (de) | 1996-11-07 |
| JP2703156B2 (ja) | 1998-01-26 |
| FI103413B1 (fi) | 1999-06-30 |
| US5643730A (en) | 1997-07-01 |
| JPH05192199A (ja) | 1993-08-03 |
| FI924242L (fi) | 1993-03-24 |
| ATE143700T1 (de) | 1996-10-15 |
| FI924242A0 (fi) | 1992-09-22 |
| DE69214243T2 (de) | 1997-02-06 |
| ES2092056T3 (es) | 1996-11-16 |
| DK0534640T3 (en:Method) | 1997-03-17 |
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