CA2051375A1 - Procede et compositions pour l'isolement de la relaxine humaine - Google Patents
Procede et compositions pour l'isolement de la relaxine humaineInfo
- Publication number
- CA2051375A1 CA2051375A1 CA2051375A CA2051375A CA2051375A1 CA 2051375 A1 CA2051375 A1 CA 2051375A1 CA 2051375 A CA2051375 A CA 2051375A CA 2051375 A CA2051375 A CA 2051375A CA 2051375 A1 CA2051375 A1 CA 2051375A1
- Authority
- CA
- Canada
- Prior art keywords
- polypeptide
- cleaving
- dna
- relaxin
- junction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract 3
- BJRCFZKVYNDCJE-WBSNEMHCSA-N 99489-95-9 Chemical compound C([C@@H]1NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@H](C(N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)NCC(=O)N2)[C@@H](C)CC)=O)CSSC[C@@H](C(NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H](CSSC[C@H](NC1=O)C(O)=O)C(=O)NCC(=O)N[C@@H](CCSC)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O)[C@@H](C)CC)[C@@H](C)CC)C(C)C)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@@H](N)CCCCN)C(C)C)[C@@H](C)CC)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CCCNC(N)=N)C(C)C)C1=CC=C(O)C=C1 BJRCFZKVYNDCJE-WBSNEMHCSA-N 0.000 title 1
- 238000002955 isolation Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 10
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 10
- 229920001184 polypeptide Polymers 0.000 abstract 9
- 108020004414 DNA Proteins 0.000 abstract 3
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 abstract 3
- 108020004705 Codon Proteins 0.000 abstract 2
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical group SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 abstract 2
- 102000003743 Relaxin Human genes 0.000 abstract 2
- 108090000103 Relaxin Proteins 0.000 abstract 2
- 239000002253 acid Substances 0.000 abstract 2
- 238000003776 cleavage reaction Methods 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 2
- 239000002243 precursor Substances 0.000 abstract 2
- 230000007017 scission Effects 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 235000003704 aspartic acid Nutrition 0.000 abstract 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/64—Relaxins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/02—Drugs for disorders of the urinary system of urine or of the urinary tract, e.g. urine acidifiers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/848—Escherichia
- Y10S435/849—Escherichia coli
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S930/00—Peptide or protein sequence
- Y10S930/01—Peptide or protein sequence
- Y10S930/31—Linker sequence
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Endocrinology (AREA)
- Public Health (AREA)
- Genetics & Genomics (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Reproductive Health (AREA)
- Urology & Nephrology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Steroid Compounds (AREA)
Abstract
L'invention concerne un procédé de clivage d'un polypeptide en au moins deux composantes du polypeptide, consistant à traiter une forme réduite, exempte de cystéine, du polypeptide, à l'aide d'un agent de clivage, dans des conditions permettant de cliver ledit polypeptide au niveau d'une jonction voulue entre les produits de clivage dudit polypeptide. Le procédé de clivage consiste de préférence à cultiver des cellules contenant de l'ADN codant ledit polypeptide, au moins un codon Asp se trouvant dans ledit ADN au niveau d'une jonction voulue entre les composantes à cliver les une des autres, ladite culture permettant d'obtenir l'expression de l'ADN pour produire ledit polypeptide dans la culture cellulaire hôte; on traite ensuite une forme réduite exempte de cystéine dudit polypeptide à l'aide d'acide dilué dans des conditions permettant de cliver ledit polypeptide au niveau de la jonction Asp. Dans des modes de réalisation particuliers, on a prévu une séquence d'ADN codant un précurseur de relaxine et comprenant des codons codant des liaisons contenant de l'acide aspartique au niveau de nouvelles positions dans le précurseur, ce qui facilite le clivage de peptides de relaxine A par traitement à l'aide d'acide dilué.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US34755089A | 1989-05-04 | 1989-05-04 | |
| US07/347,550 | 1989-05-04 | ||
| PCT/US1990/002085 WO1990013659A1 (fr) | 1989-05-04 | 1990-04-16 | Procedes et compositions d'isolement de la relaxine humaine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2051375A1 true CA2051375A1 (fr) | 1990-11-05 |
| CA2051375C CA2051375C (fr) | 2000-09-12 |
Family
ID=23364196
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002051375A Expired - Fee Related CA2051375C (fr) | 1989-05-04 | 1990-04-16 | Procede et compositions pour l'isolement de la relaxine humaine |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5464756A (fr) |
| EP (1) | EP0470976B1 (fr) |
| JP (1) | JP2857684B2 (fr) |
| AU (1) | AU623518B2 (fr) |
| CA (1) | CA2051375C (fr) |
| DE (1) | DE69001248T2 (fr) |
| WO (1) | WO1990013659A1 (fr) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69210645T2 (de) * | 1991-02-19 | 1996-11-28 | Takeda Chemical Industries, Ltd., Osaka | Verfahren zur Herstellung von cysteinfreien Peptiden |
| US5166191A (en) * | 1991-08-19 | 1992-11-24 | Genentech, Inc. | Use of relaxin in cardiovascular therapy |
| US5478807A (en) * | 1991-08-19 | 1995-12-26 | Genentech, Inc. | Use of relaxin in the treatment of bradycardia |
| CA2123430C (fr) * | 1991-12-06 | 2004-02-10 | Cornelia M. Gorman | Cellules transformees par une enzyme de conversion de prohormone |
| JP4568383B2 (ja) | 1995-08-15 | 2010-10-27 | コルセラ，インコーポレイティッド | 血管形成の促進方法 |
| US5612051A (en) * | 1995-11-17 | 1997-03-18 | Yue; Samuel K. | Method of treating involuntary muscle dysfunction with relaxin hormone |
| US6084068A (en) * | 1996-10-04 | 2000-07-04 | Oklahoma Medical Research Foundation | Elongin A and C functional domains |
| US6251863B1 (en) | 1998-09-08 | 2001-06-26 | Samuel K. Yue | Method of preventing and treating symptoms of aging and neurodegenerative dysfunctions with relaxin |
| US7049403B2 (en) * | 2000-04-21 | 2006-05-23 | Takeda Pharmaceutical Company Limited | Insulin/IGF/relaxin family polypeptides and DNAS thereof |
| US20050032683A1 (en) | 2000-10-04 | 2005-02-10 | Amento Edward P. | Methods of modulating apoptosis by administration of relaxin agonists or antagonists |
| US6439540B1 (en) | 2000-10-31 | 2002-08-27 | Pratt & Whitney Canada Corp. | Butterfly valve noise suppressor |
| DE602004014251D1 (de) * | 2003-01-31 | 2008-07-17 | Organon Nv | Verfahren zur proteinisolierung unteranoxischen bedingungen |
| AU2005247332A1 (en) * | 2004-04-30 | 2005-12-08 | Corthera, Inc. | Methods and compositions for control of fetal growth via modulation of relaxin |
| US20060052304A1 (en) * | 2004-09-02 | 2006-03-09 | Bas Medical, Inc. | Method for remodeling bone and related sutures |
| KR20080033271A (ko) * | 2005-06-17 | 2008-04-16 | 맨카인드 코포레이션 | 암종을 위한 다가 동반이행 및 증폭형 면역치료제 |
| US8445635B2 (en) * | 2006-04-11 | 2013-05-21 | Armour Therapeutics Inc. | Modified H2 relaxin for tumor suppression |
| GR1006941B (el) * | 2009-06-01 | 2010-08-27 | Χημικα Και Βιοφαρμακευτικα Εργαστηρια Πατρων Αε (Cbl-Patras), | Πεπτιδικη συνθεση (peptide synthesis) |
| WO2013004607A1 (fr) | 2011-07-01 | 2013-01-10 | Bayer Intellectual Property Gmbh | Polypeptides de fusion de relaxine et leurs utilisations |
| DK3522911T3 (da) | 2016-10-07 | 2022-03-07 | Beth Israel Deaconess Medical Ct Inc | Sammensætninger omfattende relaxin og fremgangsmåder til anvendelse deraf |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ199391A (en) * | 1981-01-02 | 1985-12-13 | Genentech Inc | Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin |
| EP0068375A3 (fr) * | 1981-06-22 | 1983-04-13 | G.D. Searle & Co. | Techniques d'ADN recombinant pour la production de relaxine |
| EP0287820B1 (fr) * | 1982-08-12 | 1992-07-08 | Howard Florey Institute Of Experimental Physiology And Medicine | Analogues de la relaxine humaine |
| NZ206534A (en) * | 1982-12-13 | 1988-05-30 | Florey Howard Inst | Molecular cloning and characterisation of gene sequence coding for human relaxin |
| DK129385A (da) * | 1985-03-22 | 1986-09-23 | Novo Industri As | Peptider og fremstilling deraf |
| US4835251A (en) * | 1986-06-23 | 1989-05-30 | Genetech, Inc. | Method of chain combination |
| DE3730599A1 (de) * | 1986-09-12 | 1988-07-07 | Genentech Inc | Verfahren zur kontinuierlichen herstellung von heterologen proteinen in eukaryontischen wirtszellen |
| US4987070A (en) * | 1987-03-04 | 1991-01-22 | Suntory Limited | Use of a 97 amino acid leader sequence from the E. coli B-galactosidase gene for the production of hanp and hptc as fusion proteins |
| ATE113985T1 (de) * | 1987-03-20 | 1994-11-15 | Creative Biomolecules Inc | Verfahren zur reinigung von rekombinanten polypeptiden. |
-
1990
- 1990-04-16 AU AU54489/90A patent/AU623518B2/en not_active Ceased
- 1990-04-16 CA CA002051375A patent/CA2051375C/fr not_active Expired - Fee Related
- 1990-04-16 EP EP90906683A patent/EP0470976B1/fr not_active Expired - Lifetime
- 1990-04-16 WO PCT/US1990/002085 patent/WO1990013659A1/fr active IP Right Grant
- 1990-04-16 DE DE90906683T patent/DE69001248T2/de not_active Expired - Fee Related
- 1990-04-16 JP JP2506493A patent/JP2857684B2/ja not_active Expired - Fee Related
-
1992
- 1992-07-01 US US07/908,766 patent/US5464756A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0470976A1 (fr) | 1992-02-19 |
| DE69001248T2 (de) | 1993-10-14 |
| US5464756A (en) | 1995-11-07 |
| EP0470976B1 (fr) | 1993-03-31 |
| AU5448990A (en) | 1990-11-29 |
| DE69001248D1 (de) | 1993-05-06 |
| JPH04504953A (ja) | 1992-09-03 |
| JP2857684B2 (ja) | 1999-02-17 |
| AU623518B2 (en) | 1992-05-14 |
| CA2051375C (fr) | 2000-09-12 |
| WO1990013659A1 (fr) | 1990-11-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |