CA2004408A1 - Methode d'identification des sequences d'adn dans les chromosomes des plantes - Google Patents
Methode d'identification des sequences d'adn dans les chromosomes des plantesInfo
- Publication number
- CA2004408A1 CA2004408A1 CA002004408A CA2004408A CA2004408A1 CA 2004408 A1 CA2004408 A1 CA 2004408A1 CA 002004408 A CA002004408 A CA 002004408A CA 2004408 A CA2004408 A CA 2004408A CA 2004408 A1 CA2004408 A1 CA 2004408A1
- Authority
- CA
- Canada
- Prior art keywords
- dna
- labelled
- target plant
- plant
- source
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 53
- 210000000349 chromosome Anatomy 0.000 title claims abstract description 36
- 108091028043 Nucleic acid sequence Proteins 0.000 title claims abstract description 28
- 108020004414 DNA Proteins 0.000 claims abstract description 167
- 239000012634 fragment Substances 0.000 claims abstract description 17
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 7
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 4
- 241000196324 Embryophyta Species 0.000 claims description 74
- 238000009396 hybridization Methods 0.000 claims description 45
- 241000209056 Secale Species 0.000 claims description 18
- 239000000523 sample Substances 0.000 claims description 17
- 239000012528 membrane Substances 0.000 claims description 16
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 14
- 241000209140 Triticum Species 0.000 claims description 11
- 235000021307 Triticum Nutrition 0.000 claims description 10
- 238000012546 transfer Methods 0.000 claims description 10
- 108020005120 Plant DNA Proteins 0.000 claims description 8
- 229960002685 biotin Drugs 0.000 claims description 7
- 235000020958 biotin Nutrition 0.000 claims description 7
- 239000011616 biotin Substances 0.000 claims description 7
- 241000209219 Hordeum Species 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 230000016507 interphase Effects 0.000 claims description 5
- 230000031864 metaphase Effects 0.000 claims description 5
- 230000002285 radioactive effect Effects 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108091008146 restriction endonucleases Proteins 0.000 claims description 3
- 238000000376 autoradiography Methods 0.000 claims description 2
- 238000001962 electrophoresis Methods 0.000 claims description 2
- 210000000416 exudates and transudate Anatomy 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 210000001519 tissue Anatomy 0.000 claims description 2
- 241000209758 Aegilops Species 0.000 claims 2
- 241000209510 Liliopsida Species 0.000 claims 1
- 238000004737 colorimetric analysis Methods 0.000 claims 1
- 230000000903 blocking effect Effects 0.000 abstract description 20
- 241001522983 Secale strictum subsp. africanum Species 0.000 description 17
- 241000744747 Hordeum chilense Species 0.000 description 13
- 235000007238 Secale cereale Nutrition 0.000 description 13
- 238000012360 testing method Methods 0.000 description 10
- 244000082988 Secale cereale Species 0.000 description 9
- 241000894007 species Species 0.000 description 9
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 6
- 238000002372 labelling Methods 0.000 description 6
- 241000499489 Castor canadensis Species 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 4
- 244000075920 Hordeum bulbosum Species 0.000 description 4
- 235000011779 Menyanthes trifoliata Nutrition 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 241000228158 x Triticosecale Species 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 108090001008 Avidin Proteins 0.000 description 3
- 240000005979 Hordeum vulgare Species 0.000 description 3
- 108020004682 Single-Stranded DNA Proteins 0.000 description 3
- 235000019714 Triticale Nutrition 0.000 description 3
- 244000098338 Triticum aestivum Species 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 238000003976 plant breeding Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- 108010077544 Chromatin Proteins 0.000 description 2
- 241001256333 Protophyta Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 244000098345 Triticum durum Species 0.000 description 2
- 210000003483 chromatin Anatomy 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000007901 in situ hybridization Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 2
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 230000016853 telophase Effects 0.000 description 2
- 230000005945 translocation Effects 0.000 description 2
- 241000123640 Arecales Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000252203 Clupea harengus Species 0.000 description 1
- 241000218631 Coniferophyta Species 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 101100046831 Drosophila melanogaster Tpst gene Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 108010044467 Isoenzymes Proteins 0.000 description 1
- 241000820057 Ithone Species 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- HRNLUBSXIHFDHP-UHFFFAOYSA-N N-(2-aminophenyl)-4-[[[4-(3-pyridinyl)-2-pyrimidinyl]amino]methyl]benzamide Chemical compound NC1=CC=CC=C1NC(=O)C(C=C1)=CC=C1CNC1=NC=CC(C=2C=NC=CC=2)=N1 HRNLUBSXIHFDHP-UHFFFAOYSA-N 0.000 description 1
- 108091093105 Nuclear DNA Proteins 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 1
- 241001263263 Secale cereale x Triticum turgidum subsp. durum Species 0.000 description 1
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 241000592344 Spermatophyta Species 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 241000592342 Tracheophyta Species 0.000 description 1
- 240000000581 Triticum monococcum Species 0.000 description 1
- 239000002981 blocking agent Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 235000019514 herring Nutrition 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 210000004020 intracellular membrane Anatomy 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000021121 meiosis Effects 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB888828130A GB8828130D0 (en) | 1988-12-02 | 1988-12-02 | Method of identifying dna sequences in chromosomes of plants |
| GB8828130.8 | 1988-12-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2004408A1 true CA2004408A1 (fr) | 1990-06-02 |
Family
ID=10647835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002004408A Abandoned CA2004408A1 (fr) | 1988-12-02 | 1989-12-01 | Methode d'identification des sequences d'adn dans les chromosomes des plantes |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0446292A1 (fr) |
| JP (1) | JPH04502253A (fr) |
| AU (1) | AU4660289A (fr) |
| CA (1) | CA2004408A1 (fr) |
| GB (1) | GB8828130D0 (fr) |
| WO (1) | WO1990006375A1 (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL97295A0 (en) * | 1990-02-27 | 1992-05-25 | Agrilab Biotechnology Ltd | Procedure for the detection of plant pathogens performed under field conditions and a diagnostic kit for its application |
| GB9321113D0 (en) * | 1993-10-13 | 1993-12-01 | Mini Agriculture & Fisheries | Government of the united kingdom of great britain and northern ireland identification of fruit juice components |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07828B2 (ja) * | 1984-10-26 | 1995-01-11 | 大同特殊鋼株式会社 | 浸炭部品 |
| JPS61253346A (ja) * | 1985-04-30 | 1986-11-11 | Kobe Steel Ltd | 高強度歯車用鋼 |
| JPS624465A (ja) * | 1985-07-01 | 1987-01-10 | Nippon Kokan Kk <Nkk> | プライマ塗布装置 |
| NZ219680A (en) * | 1986-03-20 | 1989-10-27 | Lubrizol Genetics Inc | Tomato chromosome map and cdna library |
| JPS62274052A (ja) * | 1986-05-21 | 1987-11-28 | Daido Steel Co Ltd | 軸受用肌焼鋼 |
-
1988
- 1988-12-02 GB GB888828130A patent/GB8828130D0/en active Pending
-
1989
- 1989-12-01 EP EP90900930A patent/EP0446292A1/fr not_active Withdrawn
- 1989-12-01 WO PCT/GB1989/001440 patent/WO1990006375A1/fr not_active Application Discontinuation
- 1989-12-01 JP JP2501105A patent/JPH04502253A/ja active Pending
- 1989-12-01 AU AU46602/89A patent/AU4660289A/en not_active Abandoned
- 1989-12-01 CA CA002004408A patent/CA2004408A1/fr not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JPH04502253A (ja) | 1992-04-23 |
| WO1990006375A1 (fr) | 1990-06-14 |
| EP0446292A1 (fr) | 1991-09-18 |
| GB8828130D0 (en) | 1989-01-05 |
| AU4660289A (en) | 1990-06-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |