CA1338440C - Methode pour isoler et caracteriser un systeme enzymatique genique permettant d'inactiver l'herbicide phenmediphame - Google Patents
Methode pour isoler et caracteriser un systeme enzymatique genique permettant d'inactiver l'herbicide phenmediphameInfo
- Publication number
- CA1338440C CA1338440C CA000600153A CA600153A CA1338440C CA 1338440 C CA1338440 C CA 1338440C CA 000600153 A CA000600153 A CA 000600153A CA 600153 A CA600153 A CA 600153A CA 1338440 C CA1338440 C CA 1338440C
- Authority
- CA
- Canada
- Prior art keywords
- carbamate
- arthrobacter oxidans
- dsm
- carbamate hydrolase
- arthrobacter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000010829 isocratic elution Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 108010038320 lysylphenylalanine Proteins 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- PWXJULSLLONQHY-UHFFFAOYSA-N phenylcarbamic acid Chemical compound OC(=O)NC1=CC=CC=C1 PWXJULSLLONQHY-UHFFFAOYSA-N 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 238000013492 plasmid preparation Methods 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 108010090894 prolylleucine Proteins 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 239000003774 sulfhydryl reagent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 150000003544 thiamines Chemical class 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Enzymes And Modification Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3817384.0 | 1988-05-19 | ||
| DE3817384A DE3817384A1 (de) | 1988-05-19 | 1988-05-19 | Verfahren zur isolierung einer phenmedipham spaltenden carbamat-hydrolase aus arthrobacter oxidans und anschliessenden reinigung der carbamat-hydrolase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1338440C true CA1338440C (fr) | 1996-07-09 |
Family
ID=6354873
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000600153A Expired - Fee Related CA1338440C (fr) | 1988-05-19 | 1989-05-18 | Methode pour isoler et caracteriser un systeme enzymatique genique permettant d'inactiver l'herbicide phenmediphame |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0343100B1 (fr) |
| JP (1) | JP2801263B2 (fr) |
| AT (1) | ATE108202T1 (fr) |
| CA (1) | CA1338440C (fr) |
| DE (2) | DE3817384A1 (fr) |
| DK (1) | DK175794B1 (fr) |
| ES (1) | ES2059815T3 (fr) |
| IE (1) | IE67527B1 (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2815969B1 (fr) | 2000-10-30 | 2004-12-10 | Aventis Cropscience Sa | Plantes tolerantes aux herbicides par contournement de voie metabolique |
-
1988
- 1988-05-19 DE DE3817384A patent/DE3817384A1/de not_active Withdrawn
-
1989
- 1989-05-11 DK DK198902312A patent/DK175794B1/da not_active IP Right Cessation
- 1989-05-17 AT AT89730123T patent/ATE108202T1/de not_active IP Right Cessation
- 1989-05-17 EP EP89730123A patent/EP0343100B1/fr not_active Expired - Lifetime
- 1989-05-17 ES ES89730123T patent/ES2059815T3/es not_active Expired - Lifetime
- 1989-05-17 DE DE58907998T patent/DE58907998D1/de not_active Expired - Lifetime
- 1989-05-18 IE IE160089A patent/IE67527B1/en not_active IP Right Cessation
- 1989-05-18 CA CA000600153A patent/CA1338440C/fr not_active Expired - Fee Related
- 1989-05-19 JP JP1124595A patent/JP2801263B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DK175794B1 (da) | 2005-02-21 |
| DK231289A (da) | 1989-11-20 |
| IE891600L (en) | 1989-11-19 |
| ATE108202T1 (de) | 1994-07-15 |
| DE58907998D1 (de) | 1994-08-18 |
| DE3817384A1 (de) | 1989-11-30 |
| JPH02128689A (ja) | 1990-05-17 |
| IE67527B1 (en) | 1996-04-03 |
| EP0343100B1 (fr) | 1994-07-06 |
| DK231289D0 (da) | 1989-05-11 |
| JP2801263B2 (ja) | 1998-09-21 |
| EP0343100A2 (fr) | 1989-11-23 |
| ES2059815T3 (es) | 1994-11-16 |
| EP0343100A3 (en) | 1990-05-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed |