IE891600L - Process for the isolation and characterisation of a gene¹enzyme system for inactivation of the herbicide phenmedipham - Google Patents
Process for the isolation and characterisation of a gene¹enzyme system for inactivation of the herbicide phenmediphamInfo
- Publication number
- IE891600L IE891600L IE891600A IE160089A IE891600L IE 891600 L IE891600 L IE 891600L IE 891600 A IE891600 A IE 891600A IE 160089 A IE160089 A IE 160089A IE 891600 L IE891600 L IE 891600L
- Authority
- IE
- Ireland
- Prior art keywords
- carbamate hydrolase
- phenmedipham
- characterisation
- inactivation
- isolation
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Enzymes And Modification Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to a process for the isolation and characterisation of a gene-enzyme system for the inactivation of the herbicide phenmedipham. The enzyme is a carbamate hydrolase from Arthrobacter oxidans which is responsible for the hydrolytic cleavage of the -OOC-N linkage between the two phenyl nuclei of phenmedipham. The process includes the detection of carbamate hydrolase, the identification of the amino-acid sequence of two BrCN-cleaved peptides of carbamate hydrolase, the synthesis of oligonucleotides for the specific detection of the carbamate hydrolase sequence by hybridisation, and the identification of the coding region, the cloning and the determination of the nucleotide sequence of the carbamate hydrolase gene from Arthrobacter oxidans.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3817384A DE3817384A1 (en) | 1988-05-19 | 1988-05-19 | METHOD FOR INSULATING A PHENMEDIPHAM-CLEAVING CARBAMAT-HYDROLASE FROM ARTHROBACTER OXIDANS AND FOLLOWING CLEANING OF THE CARBAMAT-HYDROLASE |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE891600L true IE891600L (en) | 1989-11-19 |
| IE67527B1 IE67527B1 (en) | 1996-04-03 |
Family
ID=6354873
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE160089A IE67527B1 (en) | 1988-05-19 | 1989-05-18 | Process for the isolation and characterisation of a gene enzyme system for inactivation of the herbicide phenmedipham |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0343100B1 (en) |
| JP (1) | JP2801263B2 (en) |
| AT (1) | ATE108202T1 (en) |
| CA (1) | CA1338440C (en) |
| DE (2) | DE3817384A1 (en) |
| DK (1) | DK175794B1 (en) |
| ES (1) | ES2059815T3 (en) |
| IE (1) | IE67527B1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2815969B1 (en) | 2000-10-30 | 2004-12-10 | Aventis Cropscience Sa | TOLERANT PLANTS WITH HERBICIDES BY METABOLIC BYPASS |
-
1988
- 1988-05-19 DE DE3817384A patent/DE3817384A1/en not_active Withdrawn
-
1989
- 1989-05-11 DK DK198902312A patent/DK175794B1/en not_active IP Right Cessation
- 1989-05-17 DE DE58907998T patent/DE58907998D1/en not_active Expired - Lifetime
- 1989-05-17 EP EP89730123A patent/EP0343100B1/en not_active Expired - Lifetime
- 1989-05-17 ES ES89730123T patent/ES2059815T3/en not_active Expired - Lifetime
- 1989-05-17 AT AT89730123T patent/ATE108202T1/en not_active IP Right Cessation
- 1989-05-18 IE IE160089A patent/IE67527B1/en not_active IP Right Cessation
- 1989-05-18 CA CA000600153A patent/CA1338440C/en not_active Expired - Fee Related
- 1989-05-19 JP JP1124595A patent/JP2801263B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0343100A3 (en) | 1990-05-30 |
| CA1338440C (en) | 1996-07-09 |
| DK231289A (en) | 1989-11-20 |
| EP0343100B1 (en) | 1994-07-06 |
| IE67527B1 (en) | 1996-04-03 |
| ES2059815T3 (en) | 1994-11-16 |
| JPH02128689A (en) | 1990-05-17 |
| DK231289D0 (en) | 1989-05-11 |
| EP0343100A2 (en) | 1989-11-23 |
| DE3817384A1 (en) | 1989-11-30 |
| DK175794B1 (en) | 2005-02-21 |
| JP2801263B2 (en) | 1998-09-21 |
| ATE108202T1 (en) | 1994-07-15 |
| DE58907998D1 (en) | 1994-08-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Patent lapsed |