CA1284959C - Sequence d'adn utile pour la production et la secretion de peptides et de proteines par des levures - Google Patents

Sequence d'adn utile pour la production et la secretion de peptides et de proteines par des levures

Info

Publication number
CA1284959C
CA1284959C CA000498511A CA498511A CA1284959C CA 1284959 C CA1284959 C CA 1284959C CA 000498511 A CA000498511 A CA 000498511A CA 498511 A CA498511 A CA 498511A CA 1284959 C CA1284959 C CA 1284959C
Authority
CA
Canada
Prior art keywords
dna
sequence
phe
promoter region
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA000498511A
Other languages
English (en)
Inventor
Nigel A. Skipper
Martin Sumner-Smith
Roger W. Davies
Richard P. Bozzato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Allelix Biopharmaceuticals Inc
Original Assignee
Allelix Biopharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Allelix Biopharmaceuticals Inc filed Critical Allelix Biopharmaceuticals Inc
Application granted granted Critical
Publication of CA1284959C publication Critical patent/CA1284959C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/244—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
CA000498511A 1984-12-24 1985-12-23 Sequence d'adn utile pour la production et la secretion de peptides et de proteines par des levures Expired - Fee Related CA1284959C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68570484A 1984-12-24 1984-12-24
US685,704 1984-12-24

Publications (1)

Publication Number Publication Date
CA1284959C true CA1284959C (fr) 1991-06-18

Family

ID=24753341

Family Applications (2)

Application Number Title Priority Date Filing Date
CA000494013A Expired CA1252256A (fr) 1984-12-24 1985-10-28 Raccord de vidage a contre-ecrou et pieces de branchement en resine synthetique
CA000498511A Expired - Fee Related CA1284959C (fr) 1984-12-24 1985-12-23 Sequence d'adn utile pour la production et la secretion de peptides et de proteines par des levures

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA000494013A Expired CA1252256A (fr) 1984-12-24 1985-10-28 Raccord de vidage a contre-ecrou et pieces de branchement en resine synthetique

Country Status (6)

Country Link
EP (1) EP0208706A1 (fr)
JP (1) JPS62502025A (fr)
AU (1) AU5304286A (fr)
CA (2) CA1252256A (fr)
DK (1) DK403186A (fr)
WO (1) WO1986003777A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5068185A (en) * 1986-07-10 1991-11-26 Merck & Co., Inc. Trains of yeast for the expression of heterologous genes
US5055401A (en) * 1987-04-10 1991-10-08 Alko Ltd. Construction of new α-galactosidase producing yeast strains and the industrial application of these strains
FR2615527B1 (fr) * 1987-05-22 1989-08-18 Lesaffre Soc Ind Procede d'integration d'une sequence connue d'adn dans les levures ascosporogenes, vecteurs mis en oeuvre et nouvelles souches de levures
DK38893D0 (da) 1993-03-31 1993-03-31 Novo Nordisk As Dna
JPH08322575A (ja) * 1995-05-30 1996-12-10 Takara Shuzo Co Ltd プロモーター
ES2351296B8 (es) * 2009-04-08 2012-07-03 Universidade Da Coruña Cepas de s. cerevisiae capaces de crecer en medios con melibiosa, estaquiosa y rafinosa.
US11692016B2 (en) * 2012-03-09 2023-07-04 Vestaron Corporation High gene expression yeast strain
DK2907393T3 (en) 2014-02-17 2018-11-12 Dmk Deutsches Milchkontor Gmbh Lactose-free milk products
CN110982721B (zh) * 2019-12-09 2022-04-26 宜昌东阳光生化制药有限公司 提高酿酒酵母代谢产物产量的方法

Also Published As

Publication number Publication date
DK403186D0 (da) 1986-08-22
DK403186A (da) 1986-08-22
JPS62502025A (ja) 1987-08-13
CA1252256A (fr) 1989-04-11
AU5304286A (en) 1986-07-22
EP0208706A1 (fr) 1987-01-21
WO1986003777A1 (fr) 1986-07-03

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