CA1284947C - Indirect colorimetric detection of an analyte in a sample using ratio of light signals - Google Patents
Indirect colorimetric detection of an analyte in a sample using ratio of light signalsInfo
- Publication number
- CA1284947C CA1284947C CA000537971A CA537971A CA1284947C CA 1284947 C CA1284947 C CA 1284947C CA 000537971 A CA000537971 A CA 000537971A CA 537971 A CA537971 A CA 537971A CA 1284947 C CA1284947 C CA 1284947C
- Authority
- CA
- Canada
- Prior art keywords
- analyte
- sample
- concentration
- light
- ratio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000012491 analyte Substances 0.000 title claims abstract description 75
- 238000001514 detection method Methods 0.000 title claims abstract description 34
- 102000004190 Enzymes Human genes 0.000 claims abstract description 48
- 108090000790 Enzymes Proteins 0.000 claims abstract description 48
- 238000000034 method Methods 0.000 claims abstract description 38
- 238000003018 immunoassay Methods 0.000 claims abstract description 26
- 239000006193 liquid solution Substances 0.000 claims abstract description 10
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 26
- 238000006243 chemical reaction Methods 0.000 claims description 21
- 238000010521 absorption reaction Methods 0.000 claims description 17
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 claims description 16
- 239000007790 solid phase Substances 0.000 claims description 14
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims description 13
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims description 13
- 229940084986 human chorionic gonadotropin Drugs 0.000 claims description 13
- 230000005284 excitation Effects 0.000 claims description 11
- 239000000725 suspension Substances 0.000 claims description 11
- 102000003992 Peroxidases Human genes 0.000 claims description 10
- 238000002835 absorbance Methods 0.000 claims description 10
- 230000001900 immune effect Effects 0.000 claims description 10
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 10
- 239000002245 particle Substances 0.000 claims description 9
- 229940127121 immunoconjugate Drugs 0.000 claims description 5
- 229920003023 plastic Polymers 0.000 claims description 5
- 230000004044 response Effects 0.000 claims description 4
- 102000009151 Luteinizing Hormone Human genes 0.000 claims description 2
- 108010073521 Luteinizing Hormone Proteins 0.000 claims description 2
- 238000001917 fluorescence detection Methods 0.000 claims description 2
- 229940040129 luteinizing hormone Drugs 0.000 claims description 2
- 239000000126 substance Substances 0.000 description 48
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 18
- 238000012360 testing method Methods 0.000 description 14
- 238000003556 assay Methods 0.000 description 13
- 239000000758 substrate Substances 0.000 description 12
- CTERCLHSWSQHSD-UHFFFAOYSA-N 8-methoxypyrene-1,3,6-trisulfonic acid Chemical compound C1=C2C(OC)=CC(S(O)(=O)=O)=C(C=C3)C2=C2C3=C(S(O)(=O)=O)C=C(S(O)(=O)=O)C2=C1 CTERCLHSWSQHSD-UHFFFAOYSA-N 0.000 description 10
- 238000000149 argon plasma sintering Methods 0.000 description 10
- 102000036639 antigens Human genes 0.000 description 9
- 108091007433 antigens Proteins 0.000 description 9
- 239000000427 antigen Substances 0.000 description 8
- 238000005259 measurement Methods 0.000 description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 7
- 229910021485 fumed silica Inorganic materials 0.000 description 7
- 239000004793 Polystyrene Substances 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 229920002223 polystyrene Polymers 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 5
- 238000000862 absorption spectrum Methods 0.000 description 4
- 230000002238 attenuated effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000006911 enzymatic reaction Methods 0.000 description 4
- 229920000136 polysorbate Polymers 0.000 description 4
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- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000012937 correction Methods 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 239000011325 microbead Substances 0.000 description 3
- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 102000011923 Thyrotropin Human genes 0.000 description 2
- 108010061174 Thyrotropin Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000012875 competitive assay Methods 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000011859 microparticle Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
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- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 229940034208 thyroxine Drugs 0.000 description 2
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 description 2
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 2
- 229910052721 tungsten Inorganic materials 0.000 description 2
- 239000010937 tungsten Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 1
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
- NUIURNJTPRWVAP-UHFFFAOYSA-N 3,3'-Dimethylbenzidine Chemical compound C1=C(N)C(C)=CC(C=2C=C(C)C(N)=CC=2)=C1 NUIURNJTPRWVAP-UHFFFAOYSA-N 0.000 description 1
- FTGPOQQGJVJDCT-UHFFFAOYSA-N 9-aminoacridine hydrochloride Chemical compound Cl.C1=CC=C2C(N)=C(C=CC=C3)C3=NC2=C1 FTGPOQQGJVJDCT-UHFFFAOYSA-N 0.000 description 1
- 238000010953 Ames test Methods 0.000 description 1
- 231100000039 Ames test Toxicity 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- HRNLUBSXIHFDHP-UHFFFAOYSA-N N-(2-aminophenyl)-4-[[[4-(3-pyridinyl)-2-pyrimidinyl]amino]methyl]benzamide Chemical compound NC1=CC=CC=C1NC(=O)C(C=C1)=CC=C1CNC1=NC=CC(C=2C=NC=CC=2)=N1 HRNLUBSXIHFDHP-UHFFFAOYSA-N 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical class C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- KCDCNGXPPGQERR-UHFFFAOYSA-N coumarin 343 Chemical compound C1CCC2=C(OC(C(C(=O)O)=C3)=O)C3=CC3=C2N1CCC3 KCDCNGXPPGQERR-UHFFFAOYSA-N 0.000 description 1
- 230000023077 detection of light stimulus Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000007421 fluorometric assay Methods 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 102000035124 heme enzymes Human genes 0.000 description 1
- 108091005655 heme enzymes Proteins 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000006194 liquid suspension Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000004848 nephelometry Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- GHTWDWCFRFTBRB-UHFFFAOYSA-M oxazine-170 Chemical compound [O-]Cl(=O)(=O)=O.N1=C2C3=CC=CC=C3C(NCC)=CC2=[O+]C2=C1C=C(C)C(N(C)CC)=C2 GHTWDWCFRFTBRB-UHFFFAOYSA-M 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- COIVODZMVVUETJ-UHFFFAOYSA-N sulforhodamine 101 Chemical compound OS(=O)(=O)C1=CC(S([O-])(=O)=O)=CC=C1C1=C(C=C2C3=C4CCCN3CCC2)C4=[O+]C2=C1C=C1CCCN3CCCC2=C13 COIVODZMVVUETJ-UHFFFAOYSA-N 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6421—Measuring at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/811—Test for named disease, body condition or organ function
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
- Y10S436/818—Human chorionic gonadotropin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- Reproductive Health (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/002,334 US4954435A (en) | 1987-01-12 | 1987-01-12 | Indirect colorimetric detection of an analyte in a sample using ratio of light signals |
| US002,334 | 1993-01-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1284947C true CA1284947C (en) | 1991-06-18 |
Family
ID=21700297
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000537971A Expired - Fee Related CA1284947C (en) | 1987-01-12 | 1987-05-26 | Indirect colorimetric detection of an analyte in a sample using ratio of light signals |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US4954435A (en, 2012) |
| EP (1) | EP0278149B1 (en, 2012) |
| JP (1) | JPH067129B2 (en, 2012) |
| AU (1) | AU600274B2 (en, 2012) |
| CA (1) | CA1284947C (en, 2012) |
| DE (1) | DE3781672T2 (en, 2012) |
| DK (1) | DK169710B1 (en, 2012) |
| ES (1) | ES2035062T3 (en, 2012) |
| FI (1) | FI88340C (en, 2012) |
| GR (1) | GR3006083T3 (en, 2012) |
| NZ (1) | NZ220508A (en, 2012) |
Families Citing this family (64)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8614084D0 (en) * | 1986-06-10 | 1986-07-16 | Serono Diagnostics Ltd | Immunoassay |
| US5093271A (en) * | 1986-11-28 | 1992-03-03 | Shimadzu Corporation | Method for the quantitative determination of antigens and antibodies by ratio of absorbances at different wavelengths |
| US5565328A (en) * | 1988-08-31 | 1996-10-15 | Dade International Inc. | Measurement of color reactions by monitoring a change of fluorescence |
| EP0396732A4 (en) * | 1988-11-14 | 1992-10-07 | Robert M. Dowben | Fluorescent immunoassays and fluorescent compounds and tracers therefore |
| US5166079A (en) * | 1989-07-19 | 1992-11-24 | Pb Diagnostic Systems, Inc. | Analytical assay method |
| CA2021658C (en) * | 1989-08-25 | 2001-10-09 | Myron J. Block | Multiplex immunoassay system |
| IT1234466B (it) * | 1989-09-20 | 1992-05-18 | Consiglio Nazionale Ricerche | Lidar a fluorescenza differenziale e relativo metodo di rilevamento |
| ES2082207T3 (es) * | 1990-06-04 | 1996-03-16 | Behring Diagnostics Inc | Ensayos para determinar analitos. |
| US5094958A (en) * | 1990-08-30 | 1992-03-10 | Fiberchem Inc. | Method of self-compensating a fiber optic chemical sensor |
| GB9027131D0 (en) * | 1990-12-14 | 1991-02-06 | Rice Evans Catherine | Diagnostic test |
| US6352672B1 (en) | 1991-01-28 | 2002-03-05 | Cis Bio International | Apparatus for measuring the luminescence emitted in a luminescent assay |
| FR2672128B1 (fr) * | 1991-01-28 | 1995-08-18 | Cis Bio Int | Procede de mesure de la luminescence emise dans un dosage par luminescence. |
| JP2912957B2 (ja) * | 1991-06-18 | 1999-06-28 | 東ソー株式会社 | 酵素活性測定方法及び装置 |
| WO1993003379A1 (en) * | 1991-07-26 | 1993-02-18 | E.I. Du Pont De Nemours And Company | An assay with signal detection in the presence of a suspended solid support |
| SE9200917D0 (sv) * | 1991-08-20 | 1992-03-25 | Pharmacia Biosensor Ab | Assay method |
| US6861264B2 (en) | 1992-01-27 | 2005-03-01 | Cis Bio International | Method of measuring the luminescence emitted in a luminescent assay |
| US5635402A (en) * | 1992-03-05 | 1997-06-03 | Alfano; Robert R. | Technique for determining whether a cell is malignant as opposed to non-malignant using extrinsic fluorescence spectroscopy |
| SE9201984D0 (sv) * | 1992-06-29 | 1992-06-29 | Pharmacia Biosensor Ab | Improvement in optical assays |
| AU698953B2 (en) * | 1994-04-29 | 1998-11-12 | Applied Biosystems, Llc | System for real time detection of nucleic acid amplification products |
| DE69530043T2 (de) * | 1994-11-08 | 2003-10-16 | Tosoh Corp., Shinnanyo | Verfahren zur Bestimmung einer fluoreszierenden Substanz, sowie Verfahren zur Enzym-Aktivität-Bestimmung |
| US6200762B1 (en) | 1997-08-01 | 2001-03-13 | Aurora Biosciences Corporation | Photon reducing agents and compositions for fluorescence assays |
| US6221612B1 (en) | 1997-08-01 | 2001-04-24 | Aurora Biosciences Corporation | Photon reducing agents for use in fluorescence assays |
| US6214563B1 (en) | 1997-08-01 | 2001-04-10 | Aurora Biosciences Corporation | Photon reducing agents for reducing undesired light emission in assays |
| US7632651B2 (en) | 1997-09-15 | 2009-12-15 | Mds Analytical Technologies (Us) Inc. | Molecular modification assays |
| US7070921B2 (en) | 2000-04-28 | 2006-07-04 | Molecular Devices Corporation | Molecular modification assays |
| US7745142B2 (en) | 1997-09-15 | 2010-06-29 | Molecular Devices Corporation | Molecular modification assays |
| US7157234B2 (en) | 1997-10-24 | 2007-01-02 | Beckman Coulter, Inc. | Detection of very low quantities of analyte bound to a solid phase |
| EP1072887B1 (en) * | 1999-07-30 | 2005-11-16 | Mitsubishi Chemical Corporation | Immunoassay |
| US7521019B2 (en) * | 2001-04-11 | 2009-04-21 | Lifescan, Inc. | Sensor device and methods for manufacture |
| US6379622B1 (en) * | 2001-04-11 | 2002-04-30 | Motorola, Inc. | Sensor incorporating a quantum dot as a reference |
| US6694158B2 (en) | 2001-04-11 | 2004-02-17 | Motorola, Inc. | System using a portable detection device for detection of an analyte through body tissue |
| WO2002090930A2 (en) * | 2001-05-09 | 2002-11-14 | Biofx Laboratories, Inc. | Stabilizing peroxides with stannous halides |
| US20030138975A1 (en) * | 2001-12-20 | 2003-07-24 | Kimberly-Clark Worldwide, Inc. | Diagnostic signal amplification with proteinoid microspheres |
| US7056535B2 (en) * | 2001-12-20 | 2006-06-06 | Kimberly-Clark Worldwide, Inc. | Triggered release from proteinoid microspheres |
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| US3527538A (en) * | 1965-08-06 | 1970-09-08 | Princeton Applied Res Corp | Absorption scattering and fluorescence measuring method and apparatus |
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| IT1129033B (it) * | 1980-09-17 | 1986-06-04 | Minnesota Mining & Mfg | Elementi fotografici a colori aventi migliorare proprieta' meccaniche |
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-
1987
- 1987-01-12 US US07/002,334 patent/US4954435A/en not_active Expired - Fee Related
- 1987-05-26 CA CA000537971A patent/CA1284947C/en not_active Expired - Fee Related
- 1987-05-27 DK DK272687A patent/DK169710B1/da not_active IP Right Cessation
- 1987-06-01 FI FI872432A patent/FI88340C/fi not_active IP Right Cessation
- 1987-06-02 NZ NZ220508A patent/NZ220508A/xx unknown
- 1987-06-03 AU AU73771/87A patent/AU600274B2/en not_active Ceased
- 1987-06-17 DE DE8787305385T patent/DE3781672T2/de not_active Expired - Fee Related
- 1987-06-17 EP EP87305385A patent/EP0278149B1/en not_active Expired
- 1987-06-17 ES ES198787305385T patent/ES2035062T3/es not_active Expired - Lifetime
- 1987-08-24 JP JP62210024A patent/JPH067129B2/ja not_active Expired - Lifetime
-
1992
- 1992-10-26 GR GR920402412T patent/GR3006083T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0278149B1 (en) | 1992-09-09 |
| EP0278149A3 (en) | 1988-11-02 |
| FI872432A0 (fi) | 1987-06-01 |
| AU600274B2 (en) | 1990-08-09 |
| AU7377187A (en) | 1988-07-14 |
| FI88340C (fi) | 1993-04-26 |
| US4954435A (en) | 1990-09-04 |
| DE3781672D1 (de) | 1992-10-15 |
| FI88340B (fi) | 1993-01-15 |
| DK272687D0 (da) | 1987-05-27 |
| JPH067129B2 (ja) | 1994-01-26 |
| ES2035062T3 (es) | 1993-04-16 |
| NZ220508A (en) | 1990-03-27 |
| DK272687A (da) | 1988-07-13 |
| DK169710B1 (da) | 1995-01-16 |
| EP0278149A2 (en) | 1988-08-17 |
| JPS63180858A (ja) | 1988-07-25 |
| FI872432A7 (fi) | 1988-07-13 |
| DE3781672T2 (de) | 1993-03-04 |
| GR3006083T3 (en, 2012) | 1993-06-21 |
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