CA1198672A - Fibrinolytically active agent and a method for the preparation thereof - Google Patents
Fibrinolytically active agent and a method for the preparation thereofInfo
- Publication number
- CA1198672A CA1198672A CA000422034A CA422034A CA1198672A CA 1198672 A CA1198672 A CA 1198672A CA 000422034 A CA000422034 A CA 000422034A CA 422034 A CA422034 A CA 422034A CA 1198672 A CA1198672 A CA 1198672A
- Authority
- CA
- Canada
- Prior art keywords
- solution
- aqueous
- earthworms
- tissues
- extract solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000013543 active substance Substances 0.000 title claims description 24
- 239000000284 extract Substances 0.000 claims abstract description 44
- 241001233061 earthworms Species 0.000 claims abstract description 40
- 239000004480 active ingredient Substances 0.000 claims abstract description 14
- 238000000746 purification Methods 0.000 claims abstract description 11
- 241000243679 Lumbricidae Species 0.000 claims abstract description 7
- 230000018044 dehydration Effects 0.000 claims abstract description 5
- 238000006297 dehydration reaction Methods 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 93
- 239000000843 powder Substances 0.000 claims description 17
- 238000000605 extraction Methods 0.000 claims description 14
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- 238000001556 precipitation Methods 0.000 claims description 10
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- 238000004587 chromatography analysis Methods 0.000 claims description 7
- 239000003495 polar organic solvent Substances 0.000 claims description 7
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- 238000001042 affinity chromatography Methods 0.000 claims description 5
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- 238000001179 sorption measurement Methods 0.000 claims description 4
- 238000003795 desorption Methods 0.000 claims 1
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- 230000000694 effects Effects 0.000 description 31
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- 235000011083 sodium citrates Nutrition 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
- GYDJEQRTZSCIOI-LJGSYFOKSA-N tranexamic acid Chemical compound NC[C@H]1CC[C@H](C(O)=O)CC1 GYDJEQRTZSCIOI-LJGSYFOKSA-N 0.000 description 1
- 229960000401 tranexamic acid Drugs 0.000 description 1
- 229940108519 trasylol Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Molecular Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Diabetes (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57031467A JPS58148824A (ja) | 1982-02-27 | 1982-02-27 | 線溶活性物質の製造法 |
| JP31467/1982 | 1982-02-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1198672A true CA1198672A (en) | 1985-12-31 |
Family
ID=12332056
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000422034A Expired CA1198672A (en) | 1982-02-27 | 1983-02-21 | Fibrinolytically active agent and a method for the preparation thereof |
Country Status (6)
| Country | Link |
|---|---|
| JP (1) | JPS58148824A (enrdf_load_stackoverflow) |
| CA (1) | CA1198672A (enrdf_load_stackoverflow) |
| DE (1) | DE3306944A1 (enrdf_load_stackoverflow) |
| FR (1) | FR2522266B1 (enrdf_load_stackoverflow) |
| GB (1) | GB2116565B (enrdf_load_stackoverflow) |
| IT (1) | IT1197586B (enrdf_load_stackoverflow) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU1629383A (en) * | 1982-10-02 | 1984-04-05 | Amano Seiyaku K.K. | Earthworm tissue protease thrombolytic agents |
| DE3519736C2 (de) * | 1985-06-01 | 1995-11-16 | Haeusler Gerd | Mittel zur Behandlung von Rheuma und Polyarthritis |
| US5024844A (en) * | 1987-08-18 | 1991-06-18 | Eimei Company, Ltd. | Process for the production of dried earthworm powder and antihyperlipemic, antidiabetic, antihypertensive and antihypotensive preparations containing dried earthworm powder as active ingredient |
| KR900012617A (ko) * | 1987-10-28 | 1990-09-01 | 요오이찌 이시이 | 혈전증 치료제 및 그 제조방법 |
| IT1230719B (it) * | 1988-04-19 | 1991-10-29 | Eimei Co Ltd | Procedimento per la produzione di polvere di lombrichi secca e preparazioni anti iperlipemiche, anti diabetiche, anti ipertensive ed anti ipotensive contenenti, come ingrediente attivo, polvere secca di lombrichi. |
| US5186944A (en) * | 1989-02-15 | 1993-02-16 | Eimei Company Ltd. | Therapeutic medicament for thrombosis |
| JP2006096673A (ja) * | 2004-09-28 | 2006-04-13 | Mihara Lr Kenkyusho:Kk | ミミズ製品の製造方法 |
| JP2009143845A (ja) * | 2007-12-14 | 2009-07-02 | Mihara Lr Kenkyusho:Kk | ミミズ抽出液の製造方法及びミミズ乾燥粉末の製造方法 |
| EP2255819A1 (en) * | 2009-05-26 | 2010-12-01 | Inmobiliaria Algeciras Ltda | Extract of annelid and use thereof for the regeneration of the skin |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU1629383A (en) * | 1982-10-02 | 1984-04-05 | Amano Seiyaku K.K. | Earthworm tissue protease thrombolytic agents |
-
1982
- 1982-02-27 JP JP57031467A patent/JPS58148824A/ja active Granted
-
1983
- 1983-02-21 CA CA000422034A patent/CA1198672A/en not_active Expired
- 1983-02-25 GB GB08305359A patent/GB2116565B/en not_active Expired
- 1983-02-25 IT IT47795/83A patent/IT1197586B/it active
- 1983-02-25 FR FR8303165A patent/FR2522266B1/fr not_active Expired
- 1983-02-28 DE DE19833306944 patent/DE3306944A1/de active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| GB2116565B (en) | 1985-02-27 |
| IT1197586B (it) | 1988-12-06 |
| FR2522266A1 (fr) | 1983-09-02 |
| JPS645576B2 (enrdf_load_stackoverflow) | 1989-01-31 |
| DE3306944A1 (de) | 1983-09-15 |
| JPS58148824A (ja) | 1983-09-05 |
| GB2116565A (en) | 1983-09-28 |
| DE3306944C2 (enrdf_load_stackoverflow) | 1989-04-13 |
| GB8305359D0 (en) | 1983-03-30 |
| FR2522266B1 (fr) | 1987-07-31 |
| IT8347795A0 (it) | 1983-02-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |