CA1198385A - Optimal polypeptide production - Google Patents
Optimal polypeptide productionInfo
- Publication number
- CA1198385A CA1198385A CA000373031A CA373031A CA1198385A CA 1198385 A CA1198385 A CA 1198385A CA 000373031 A CA000373031 A CA 000373031A CA 373031 A CA373031 A CA 373031A CA 1198385 A CA1198385 A CA 1198385A
- Authority
- CA
- Canada
- Prior art keywords
- gene
- polypeptide
- fused
- region
- coding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 68
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 68
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 67
- 238000004519 manufacturing process Methods 0.000 title description 8
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 118
- 238000000034 method Methods 0.000 claims abstract description 25
- 244000005700 microbiome Species 0.000 claims abstract description 14
- 238000013519 translation Methods 0.000 claims abstract description 13
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 8
- 238000010367 cloning Methods 0.000 claims abstract description 5
- 239000012634 fragment Substances 0.000 claims description 32
- 241000588724 Escherichia coli Species 0.000 claims description 6
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 5
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 5
- 108700009124 Transcription Initiation Site Proteins 0.000 claims description 2
- 239000000203 mixture Substances 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 description 15
- 230000004927 fusion Effects 0.000 description 10
- 230000014616 translation Effects 0.000 description 10
- 108020004414 DNA Proteins 0.000 description 5
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 238000003556 assay Methods 0.000 description 4
- 101150109249 lacI gene Proteins 0.000 description 4
- 101150066555 lacZ gene Proteins 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- 230000006798 recombination Effects 0.000 description 4
- 238000005215 recombination Methods 0.000 description 3
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000003471 mutagenic agent Substances 0.000 description 2
- 231100000707 mutagenic chemical Toxicity 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000027294 Fusi Species 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 235000019687 Lamb Nutrition 0.000 description 1
- 101100370749 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) trpC1 gene Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 239000006035 Tryptophane Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000002962 chemical mutagen Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 108010052305 exodeoxyribonuclease III Proteins 0.000 description 1
- 102000018146 globin Human genes 0.000 description 1
- 108060003196 globin Proteins 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- XKLJHFLUAHKGGU-UHFFFAOYSA-N nitrous amide Chemical class ON=N XKLJHFLUAHKGGU-UHFFFAOYSA-N 0.000 description 1
- 238000003168 reconstitution method Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 101150016309 trpC gene Proteins 0.000 description 1
- 101150100816 trpD gene Proteins 0.000 description 1
- 101150079930 trpGD gene Proteins 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13115280A | 1980-03-17 | 1980-03-17 | |
| US131,152 | 1980-03-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1198385A true CA1198385A (en) | 1985-12-24 |
Family
ID=22448125
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000373031A Expired CA1198385A (en) | 1980-03-17 | 1981-03-16 | Optimal polypeptide production |
Country Status (11)
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4874702A (en) * | 1980-09-08 | 1989-10-17 | Biogen, Inc. | Vectors and methods for making such vectors and for expressive cloned genes |
| FI64813C (fi) * | 1980-12-31 | 1984-01-10 | Ilkka Antero Palva | Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer |
| IL66263A (en) * | 1981-08-10 | 1985-12-31 | Genex Corp | Expression vectors for introducing a gene into a procaryotic organism |
| US4532207A (en) * | 1982-03-19 | 1985-07-30 | G. D. Searle & Co. | Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase |
| WO1983004262A1 (en) * | 1982-05-25 | 1983-12-08 | Brandeis University | Method of producing protein fragments |
| US4582800A (en) * | 1982-07-12 | 1986-04-15 | Hoffmann-La Roche Inc. | Novel vectors and method for controlling interferon expression |
| JPS5939899A (ja) * | 1982-08-27 | 1984-03-05 | Gakuzo Tamura | 新規ベクタ− |
| EP0130074B1 (en) * | 1983-06-27 | 1991-02-06 | Genentech, Inc. | Portable inducible control system, expression vectors containing them, microorganisms transformed with them, and their use in expressing exogenous protein |
| ATE100494T1 (de) * | 1984-02-08 | 1994-02-15 | Cetus Oncology Corp | Kontrollsysteme fuer rekombinant-manipulationen. |
| US4923808A (en) * | 1985-03-12 | 1990-05-08 | Genentech, Inc. | Method for identifying mutants secreting high levels of heterologous proteins |
| IL78775A (en) * | 1985-05-15 | 1992-06-21 | Biotech Australia Pty Ltd | Oral vaccines |
| US5030576A (en) * | 1986-04-30 | 1991-07-09 | Genentech, Inc. | Receptors for efficient determination of ligands and their antagonists or agonists |
| US4859609A (en) * | 1986-04-30 | 1989-08-22 | Genentech, Inc. | Novel receptors for efficient determination of ligands and their antagonists or agonists |
| WO2016208385A1 (ja) | 2015-06-23 | 2016-12-29 | 富士フイルム株式会社 | 電気音響変換フィルムおよび電気音響変換器 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1166983A (en) * | 1977-11-08 | 1984-05-08 | Arthur D. Riggs | Method and means for microbial polypeptide expression |
| IE48385B1 (en) * | 1978-08-11 | 1984-12-26 | Univ California | Synthesis of a eucaryotic protein by a microorganism |
| NL8000127A (nl) * | 1979-01-15 | 1980-07-17 | Harvard College | Werkwijze voor het vormen van prokaryotische of eukaryotische proteinen alsmede een daarbij toepasbaar gekoppeld gen. |
-
1981
- 1981-03-16 ES ES500397A patent/ES8205432A1/es not_active Expired
- 1981-03-16 DE DE19813153267 patent/DE3153267A1/de active Pending
- 1981-03-16 CA CA000373031A patent/CA1198385A/en not_active Expired
- 1981-03-16 GB GB8108136A patent/GB2071671B/en not_active Expired
- 1981-03-16 DE DE19813110031 patent/DE3110031A1/de not_active Withdrawn
- 1981-03-16 SE SE8101665A patent/SE8101665L/ not_active Application Discontinuation
- 1981-03-16 IT IT67359/81A patent/IT1144705B/it active
- 1981-03-17 BE BE0/204159A patent/BE887991A/fr not_active IP Right Cessation
- 1981-03-17 JP JP3733881A patent/JPS56167698A/ja active Pending
- 1981-03-17 AT AT0122281A patent/AT378004B/de not_active IP Right Cessation
- 1981-03-17 NL NL8101289A patent/NL8101289A/nl not_active Application Discontinuation
- 1981-03-17 FR FR8105347A patent/FR2478125B1/fr not_active Expired
-
1982
- 1982-03-01 ES ES510007A patent/ES510007A0/es active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS56167698A (en) | 1981-12-23 |
| BE887991A (fr) | 1981-07-16 |
| IT8167359A0 (it) | 1981-03-16 |
| SE8101665L (sv) | 1981-09-18 |
| FR2478125A1 (fr) | 1981-09-18 |
| ES500397A0 (es) | 1982-06-01 |
| DE3110031A1 (de) | 1982-01-07 |
| ES8306177A1 (es) | 1983-05-01 |
| ATA122281A (de) | 1984-10-15 |
| ES8205432A1 (es) | 1982-06-01 |
| NL8101289A (nl) | 1981-10-16 |
| GB2071671B (en) | 1983-10-05 |
| AT378004B (de) | 1985-06-10 |
| GB2071671A (en) | 1981-09-23 |
| DE3153267A1 (enrdf_load_stackoverflow) | 1985-08-14 |
| FR2478125B1 (fr) | 1985-07-05 |
| IT1144705B (it) | 1986-10-29 |
| ES510007A0 (es) | 1983-05-01 |
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| CA1198385A (en) | Optimal polypeptide production | |
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| US4952501A (en) | Method for analyzing and synthesizing functional polypeptide sequences for assembly into enhanced or multi-functional proteins | |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKEX | Expiry |