CA1125634A - Amylase determination - Google Patents
Amylase determinationInfo
- Publication number
- CA1125634A CA1125634A CA303,308A CA303308A CA1125634A CA 1125634 A CA1125634 A CA 1125634A CA 303308 A CA303308 A CA 303308A CA 1125634 A CA1125634 A CA 1125634A
- Authority
- CA
- Canada
- Prior art keywords
- amylase
- alpha
- nitrophenol
- glucosidase
- chromophore
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000004382 Amylase Substances 0.000 title claims abstract description 41
- 102000013142 Amylases Human genes 0.000 title claims abstract description 32
- 108010065511 Amylases Proteins 0.000 title claims abstract description 32
- 235000019418 amylase Nutrition 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 40
- BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical class OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 150000002482 oligosaccharides Polymers 0.000 claims abstract description 16
- 230000000694 effects Effects 0.000 claims abstract description 15
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 15
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims abstract description 4
- 239000000758 substrate Substances 0.000 claims description 16
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 8
- HSHNITRMYYLLCV-UHFFFAOYSA-N 4-methylumbelliferone Chemical compound C1=C(O)C=CC2=C1OC(=O)C=C2C HSHNITRMYYLLCV-UHFFFAOYSA-N 0.000 claims description 7
- 238000003556 assay Methods 0.000 claims description 7
- 102000004366 Glucosidases Human genes 0.000 claims description 6
- 108010056771 Glucosidases Proteins 0.000 claims description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 3
- 239000008057 potassium phosphate buffer Substances 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims 2
- 238000005065 mining Methods 0.000 claims 1
- 229910000160 potassium phosphate Inorganic materials 0.000 claims 1
- 235000011009 potassium phosphates Nutrition 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 abstract description 9
- 230000007017 scission Effects 0.000 abstract description 9
- 239000012634 fragment Substances 0.000 abstract description 3
- 238000003745 diagnosis Methods 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract 2
- 238000005259 measurement Methods 0.000 abstract 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 150000004676 glycans Chemical class 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- WQDUMFSSJAZKTM-UHFFFAOYSA-N Sodium methoxide Chemical compound [Na+].[O-]C WQDUMFSSJAZKTM-UHFFFAOYSA-N 0.000 description 4
- 229920002472 Starch Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 235000019698 starch Nutrition 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 229910021627 Tin(IV) chloride Inorganic materials 0.000 description 2
- 102000016679 alpha-Glucosidases Human genes 0.000 description 2
- 108010028144 alpha-Glucosidases Proteins 0.000 description 2
- 239000013060 biological fluid Substances 0.000 description 2
- 230000006196 deacetylation Effects 0.000 description 2
- 238000003381 deacetylation reaction Methods 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 150000004804 polysaccharides Polymers 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- HPGGPRDJHPYFRM-UHFFFAOYSA-J tin(iv) chloride Chemical compound Cl[Sn](Cl)(Cl)Cl HPGGPRDJHPYFRM-UHFFFAOYSA-J 0.000 description 2
- DRTQHJPVMGBUCF-UCVXFZOQSA-N 1-[(2s,3s,4s,5s)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione Chemical compound O[C@H]1[C@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UCVXFZOQSA-N 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 208000016222 Pancreatic disease Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical class [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 1
- LUEWUZLMQUOBSB-UHFFFAOYSA-N UNPD55895 Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(OC3C(OC(O)C(O)C3O)CO)C(O)C2O)CO)C(O)C1O LUEWUZLMQUOBSB-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000021736 acetylation Effects 0.000 description 1
- 238000006640 acetylation reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- WGLUMOCWFMKWIL-UHFFFAOYSA-N dichloromethane;methanol Chemical compound OC.ClCCl WGLUMOCWFMKWIL-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000921 elemental analysis Methods 0.000 description 1
- DEQYTNZJHKPYEZ-UHFFFAOYSA-N ethyl acetate;heptane Chemical compound CCOC(C)=O.CCCCCCC DEQYTNZJHKPYEZ-UHFFFAOYSA-N 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- UYQJCPNSAVWAFU-UHFFFAOYSA-N malto-tetraose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(O)C(CO)O2)O)C(CO)O1 UYQJCPNSAVWAFU-UHFFFAOYSA-N 0.000 description 1
- LUEWUZLMQUOBSB-OUBHKODOSA-N maltotetraose Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O[C@@H]3[C@@H](O[C@@H](O)[C@H](O)[C@H]3O)CO)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-OUBHKODOSA-N 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 125000000740 n-pentyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000000135 prohibitive effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/820,072 US4102747A (en) | 1977-07-28 | 1977-07-28 | Amylase determination |
| US820,072 | 1977-07-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1125634A true CA1125634A (en) | 1982-06-15 |
Family
ID=25229814
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA303,308A Expired CA1125634A (en) | 1977-07-28 | 1978-05-15 | Amylase determination |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US4102747A (enExample) |
| JP (1) | JPS5425893A (enExample) |
| BE (1) | BE869294A (enExample) |
| CA (1) | CA1125634A (enExample) |
| CH (1) | CH633583A5 (enExample) |
| DE (1) | DE2822364A1 (enExample) |
| FR (1) | FR2399025A1 (enExample) |
| GB (1) | GB1571265A (enExample) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2755803A1 (de) * | 1977-12-14 | 1979-06-21 | Boehringer Mannheim Gmbh | Verfahren und reagens zur bestimmung von alpha-amylase |
| DE2741192C2 (de) * | 1977-09-13 | 1982-07-01 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur Bestimmung von alpha- Amylase |
| AT362526B (de) * | 1977-09-13 | 1981-05-25 | Boehringer Mannheim Gmbh | Verfahren und reagens zur bestimmung von alpha- -amylase |
| US4225672A (en) * | 1979-02-27 | 1980-09-30 | Hall Leo M | Method for producing maltooligosaccharide glycosides |
| US4550077A (en) * | 1980-05-07 | 1985-10-29 | Electro-Nucleonics, Inc. | β-Amylase determination |
| US4451563A (en) * | 1981-08-28 | 1984-05-29 | Kaufman Richard A | Method for increasing the sensitivity of assays |
| US4472499A (en) * | 1982-01-22 | 1984-09-18 | American Hoechst Corporation | Reagents for the determination of enzymes |
| JPS58221283A (ja) * | 1982-06-15 | 1983-12-22 | Nisshin Steel Co Ltd | ステンレス鋼着色前処理方法 |
| JPS602199A (ja) * | 1983-06-21 | 1985-01-08 | Toyobo Co Ltd | α−アミラ−ゼ活性測定法 |
| DE3323245A1 (de) * | 1983-06-28 | 1985-01-10 | Merck Patent Gmbh, 6100 Darmstadt | Verfahren und reagenz zur bestimmung von (alpha)-amylase |
| DE3328616A1 (de) * | 1983-08-08 | 1985-02-28 | Boehringer Mannheim Gmbh, 6800 Mannheim | Oligoglucosidderivate |
| US4794078A (en) * | 1984-07-26 | 1988-12-27 | Genzyme Corporation | Alpha amylase assay |
| US4649108A (en) * | 1984-07-26 | 1987-03-10 | Genzyme Corporation | Alpha amylase assay |
| US5011964A (en) * | 1985-03-07 | 1991-04-30 | Cornell Research Foundation, Inc. | Novel diacylglycerophosphoric acid esters and use thereof as substrates in enzyme assays |
| DE3508384A1 (de) * | 1985-03-08 | 1986-11-06 | Boehringer Mannheim Gmbh, 6800 Mannheim | Hemmung humaner speichel- und pankreasamylase durch monoklonale antikoerper |
| JPS62138198A (ja) * | 1985-12-10 | 1987-06-20 | Fuji Photo Film Co Ltd | 自己顕色性基質を含む乾式液体分析要素 |
| US4963479A (en) * | 1986-10-07 | 1990-10-16 | Hoechst Celanese Corporation | Reagent system for an alpha-amylase assay containing aromatic substituted glycoside |
| US5158872A (en) * | 1986-10-07 | 1992-10-27 | Hoechst Celanese Corporation | Aromatic substituted glycoside |
| DE3854127T2 (de) * | 1987-07-17 | 1995-11-16 | Modrovich Ivan Endre | Stabiles alpha-amylase-reagenz. |
| DE3743908A1 (de) * | 1987-12-23 | 1989-07-06 | Boehringer Mannheim Gmbh | Neue oligoglucoside |
| US4990445A (en) * | 1988-01-20 | 1991-02-05 | Beckman Instruments, Inc. | Stable reagent and kinetic assay for alpha-amylase |
| JPH0676431B2 (ja) * | 1988-06-17 | 1994-09-28 | 東洋紡績株式会社 | 糖エステル誘導体および加水分解酵素活性測定用試薬 |
| US5086001A (en) * | 1989-12-01 | 1992-02-04 | Baxter International, Inc. | Automated test method for evaluating the physical compatibility of intravenous drugs in solutions |
| CA2039564A1 (en) * | 1990-04-10 | 1991-10-11 | James R. Rasmussen | Oligosaccharide compounds and preparation thereof |
| JP2786336B2 (ja) * | 1991-03-04 | 1998-08-13 | 富士写真フイルム株式会社 | 免疫分析要素および免疫分析方法 |
| JP3075377B2 (ja) * | 1991-11-06 | 2000-08-14 | 東洋紡績株式会社 | α−アミラーゼ活性の測定法およびα−アミラーゼ活性測定用試薬 |
| US5766872A (en) * | 1995-11-20 | 1998-06-16 | Dade International Inc. | Method for eliminating hemolysis interference in an amylase analysis |
| CA2787021A1 (en) * | 2009-02-05 | 2010-08-12 | Hydradx, Inc. | Diagnostic device and method |
| CN105319175B (zh) * | 2015-11-03 | 2017-10-31 | 浙江大学 | 中药提取过程动态响应模型的在线识别与终点判定方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1508496A (fr) * | 1966-01-20 | 1968-01-05 | Warner Lambert Pharmaceutical | Produit et procédé pour le dosage de l'amylase |
| US3694318A (en) * | 1968-08-19 | 1972-09-26 | Bernard Klein | Substrate and method for alpha-amylase assay |
| US3679661A (en) * | 1970-12-21 | 1972-07-25 | Warner Lambert Co | Preparation of water-soluble,dyed substrates for amylase assay |
| JPS5185790A (en) * | 1975-01-24 | 1976-07-27 | Ono Pharmaceutical Co | Hitononyooyobitaiekinogurukoamiraazenobiryoteiryoho |
| CA1104912A (en) * | 1976-07-13 | 1981-07-14 | Robert C. Menson | Method and test kit for serum amylase assay |
-
1977
- 1977-07-28 US US05/820,072 patent/US4102747A/en not_active Expired - Lifetime
-
1978
- 1978-04-11 GB GB14182/78A patent/GB1571265A/en not_active Expired
- 1978-05-03 FR FR7813052A patent/FR2399025A1/fr active Granted
- 1978-05-15 CA CA303,308A patent/CA1125634A/en not_active Expired
- 1978-05-23 DE DE19782822364 patent/DE2822364A1/de not_active Withdrawn
- 1978-05-31 JP JP6561278A patent/JPS5425893A/ja active Pending
- 1978-06-26 CH CH693178A patent/CH633583A5/fr not_active IP Right Cessation
- 1978-07-26 BE BE6046551A patent/BE869294A/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| BE869294A (fr) | 1979-01-26 |
| CH633583A5 (fr) | 1982-12-15 |
| JPS5425893A (en) | 1979-02-27 |
| FR2399025B1 (enExample) | 1984-03-09 |
| DE2822364A1 (de) | 1979-02-08 |
| US4102747A (en) | 1978-07-25 |
| FR2399025A1 (fr) | 1979-02-23 |
| GB1571265A (en) | 1980-07-09 |
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