FR2399025A1 - Procede de determination de l'amylase - Google Patents

Procede de determination de l'amylase

Info

Publication number
FR2399025A1
FR2399025A1 FR7813052A FR7813052A FR2399025A1 FR 2399025 A1 FR2399025 A1 FR 2399025A1 FR 7813052 A FR7813052 A FR 7813052A FR 7813052 A FR7813052 A FR 7813052A FR 2399025 A1 FR2399025 A1 FR 2399025A1
Authority
FR
France
Prior art keywords
amylase
alpha
chromophore
glucosidase
beta
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7813052A
Other languages
English (en)
Other versions
FR2399025B1 (fr
Inventor
Richard C Driscoll
Robert J Gargiulo
Joseph L Giegel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
American Hospital Supply Corp
Original Assignee
American Hospital Supply Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Hospital Supply Corp filed Critical American Hospital Supply Corp
Publication of FR2399025A1 publication Critical patent/FR2399025A1/fr
Application granted granted Critical
Publication of FR2399025B1 publication Critical patent/FR2399025B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé pour la détermination de l'amylase. Pour déterminer l'amylase on clive des substances chromogènes dont le chromophore est une substance colorimétrique fluorescente ou absorbant les ultraviolets, telles que des dérivés du p-nitrophénol et d'oligosaccharides dont la chaîne comporte 4 à 10 motifs de glucose; les oligosaccharides dont la chaîne a une telle longueur résistent au clivage par l' alpha - et/ou la beta -glucosidase et comportent des liaisons endo- alpha -1,4 nécessaires à la manifestation de l'activité amylasique; le clivage des liaisons alpha -1,4 par l'amylase forme des fragments plus petits sur lesquels l' alpha -glucosidase et/ou la beta -glucosidase agissent pour libérer le chromophore; la vitesse de libération du chromophore est proponionnelle à l'activité amylasique et permet des déterminations selon une méthode cinétique ou une méthode du point final. L'invention s'applique en particulier à des déterminations qualitatives ou quantitatives de l'amylase dans des échantillons utiles pour le diagnostic médical.
FR7813052A 1977-07-28 1978-05-03 Procede de determination de l'amylase Granted FR2399025A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US05/820,072 US4102747A (en) 1977-07-28 1977-07-28 Amylase determination

Publications (2)

Publication Number Publication Date
FR2399025A1 true FR2399025A1 (fr) 1979-02-23
FR2399025B1 FR2399025B1 (fr) 1984-03-09

Family

ID=25229814

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7813052A Granted FR2399025A1 (fr) 1977-07-28 1978-05-03 Procede de determination de l'amylase

Country Status (8)

Country Link
US (1) US4102747A (fr)
JP (1) JPS5425893A (fr)
BE (1) BE869294A (fr)
CA (1) CA1125634A (fr)
CH (1) CH633583A5 (fr)
DE (1) DE2822364A1 (fr)
FR (1) FR2399025A1 (fr)
GB (1) GB1571265A (fr)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT362526B (de) * 1977-09-13 1981-05-25 Boehringer Mannheim Gmbh Verfahren und reagens zur bestimmung von alpha- -amylase
DE2741192C2 (de) * 1977-09-13 1982-07-01 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur Bestimmung von alpha- Amylase
DE2755803A1 (de) * 1977-12-14 1979-06-21 Boehringer Mannheim Gmbh Verfahren und reagens zur bestimmung von alpha-amylase
US4225672A (en) * 1979-02-27 1980-09-30 Hall Leo M Method for producing maltooligosaccharide glycosides
US4550077A (en) * 1980-05-07 1985-10-29 Electro-Nucleonics, Inc. β-Amylase determination
US4451563A (en) * 1981-08-28 1984-05-29 Kaufman Richard A Method for increasing the sensitivity of assays
US4472499A (en) * 1982-01-22 1984-09-18 American Hoechst Corporation Reagents for the determination of enzymes
JPS58221283A (ja) * 1982-06-15 1983-12-22 Nisshin Steel Co Ltd ステンレス鋼着色前処理方法
JPS602199A (ja) * 1983-06-21 1985-01-08 Toyobo Co Ltd α−アミラ−ゼ活性測定法
DE3323245A1 (de) * 1983-06-28 1985-01-10 Merck Patent Gmbh, 6100 Darmstadt Verfahren und reagenz zur bestimmung von (alpha)-amylase
DE3328616A1 (de) * 1983-08-08 1985-02-28 Boehringer Mannheim Gmbh, 6800 Mannheim Oligoglucosidderivate
US4649108A (en) * 1984-07-26 1987-03-10 Genzyme Corporation Alpha amylase assay
US4794078A (en) * 1984-07-26 1988-12-27 Genzyme Corporation Alpha amylase assay
US5011964A (en) * 1985-03-07 1991-04-30 Cornell Research Foundation, Inc. Novel diacylglycerophosphoric acid esters and use thereof as substrates in enzyme assays
DE3508384A1 (de) * 1985-03-08 1986-11-06 Boehringer Mannheim Gmbh, 6800 Mannheim Hemmung humaner speichel- und pankreasamylase durch monoklonale antikoerper
JPS62138198A (ja) * 1985-12-10 1987-06-20 Fuji Photo Film Co Ltd 自己顕色性基質を含む乾式液体分析要素
US4963479A (en) * 1986-10-07 1990-10-16 Hoechst Celanese Corporation Reagent system for an alpha-amylase assay containing aromatic substituted glycoside
US5158872A (en) * 1986-10-07 1992-10-27 Hoechst Celanese Corporation Aromatic substituted glycoside
WO1989000600A1 (fr) * 1987-07-17 1989-01-26 Ivan Endre Modrovich Reactif liquide stable monoconstituant de dosage de l'alpha-amylase
DE3743908A1 (de) * 1987-12-23 1989-07-06 Boehringer Mannheim Gmbh Neue oligoglucoside
US4990445A (en) * 1988-01-20 1991-02-05 Beckman Instruments, Inc. Stable reagent and kinetic assay for alpha-amylase
JPH0676431B2 (ja) * 1988-06-17 1994-09-28 東洋紡績株式会社 糖エステル誘導体および加水分解酵素活性測定用試薬
US5086001A (en) * 1989-12-01 1992-02-04 Baxter International, Inc. Automated test method for evaluating the physical compatibility of intravenous drugs in solutions
CA2039564A1 (fr) * 1990-04-10 1991-10-11 James R. Rasmussen Composes de l'oligosaccharide et leur preparation
JP2786336B2 (ja) * 1991-03-04 1998-08-13 富士写真フイルム株式会社 免疫分析要素および免疫分析方法
JP3075377B2 (ja) * 1991-11-06 2000-08-14 東洋紡績株式会社 α−アミラーゼ活性の測定法およびα−アミラーゼ活性測定用試薬
US5766872A (en) * 1995-11-20 1998-06-16 Dade International Inc. Method for eliminating hemolysis interference in an amylase analysis
WO2010090810A2 (fr) * 2009-02-05 2010-08-12 Hydradx, Inc. Dispositif et procédé de diagnostic
CN105319175B (zh) * 2015-11-03 2017-10-31 浙江大学 中药提取过程动态响应模型的在线识别与终点判定方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1508496A (fr) * 1966-01-20 1968-01-05 Warner Lambert Pharmaceutical Produit et procédé pour le dosage de l'amylase
US3694318A (en) * 1968-08-19 1972-09-26 Bernard Klein Substrate and method for alpha-amylase assay
US4009079A (en) * 1975-01-24 1977-02-22 Ono Pharmaceutical Co., Ltd. Method for quantitatively determining glucoamylase in human urine and body fluids
FR2358660A1 (fr) * 1976-07-13 1978-02-10 Du Pont Procede et necessaire d'essai pour dosage de l'amylase du serum

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3679661A (en) * 1970-12-21 1972-07-25 Warner Lambert Co Preparation of water-soluble,dyed substrates for amylase assay

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1508496A (fr) * 1966-01-20 1968-01-05 Warner Lambert Pharmaceutical Produit et procédé pour le dosage de l'amylase
US3694318A (en) * 1968-08-19 1972-09-26 Bernard Klein Substrate and method for alpha-amylase assay
US4009079A (en) * 1975-01-24 1977-02-22 Ono Pharmaceutical Co., Ltd. Method for quantitatively determining glucoamylase in human urine and body fluids
FR2358660A1 (fr) * 1976-07-13 1978-02-10 Du Pont Procede et necessaire d'essai pour dosage de l'amylase du serum

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
EXBK/58 *

Also Published As

Publication number Publication date
JPS5425893A (en) 1979-02-27
CA1125634A (fr) 1982-06-15
BE869294A (fr) 1979-01-26
DE2822364A1 (de) 1979-02-08
CH633583A5 (fr) 1982-12-15
GB1571265A (en) 1980-07-09
FR2399025B1 (fr) 1984-03-09
US4102747A (en) 1978-07-25

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