BRPI0822510B8 - método de preparação e uso de proteínas de lise de células vero, e kit de ensaio para a determinação de hcps de células vero. - Google Patents

método de preparação e uso de proteínas de lise de células vero, e kit de ensaio para a determinação de hcps de células vero.

Info

Publication number
BRPI0822510B8
BRPI0822510B8 BRPI0822510A BRPI0822510A BRPI0822510B8 BR PI0822510 B8 BRPI0822510 B8 BR PI0822510B8 BR PI0822510 A BRPI0822510 A BR PI0822510A BR PI0822510 A BRPI0822510 A BR PI0822510A BR PI0822510 B8 BRPI0822510 B8 BR PI0822510B8
Authority
BR
Brazil
Prior art keywords
vero cell
preparation
vero
cell lysis
lysis proteins
Prior art date
Application number
BRPI0822510A
Other languages
English (en)
Inventor
Shi Jin
Shi Songming
Liu Weixu
Liu Yi
Yao Yue
Cui Zhiying
Original Assignee
Shanghai Zerun Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN2008100433197A external-priority patent/CN101570566B/zh
Priority claimed from CN2008100433182A external-priority patent/CN101571549B/zh
Application filed by Shanghai Zerun Biotech Co Ltd filed Critical Shanghai Zerun Biotech Co Ltd
Publication of BRPI0822510A2 publication Critical patent/BRPI0822510A2/pt
Publication of BRPI0822510B1 publication Critical patent/BRPI0822510B1/pt
Publication of BRPI0822510B8 publication Critical patent/BRPI0822510B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32411—Hepatovirus, i.e. hepatitis A virus
    • C12N2770/32451—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

proteínas de lise de células vero, método de sua preparação e kit de ensaio para a determinação de hcps de células vero compreendendo-as. a presente invenção proteínas de lise de células vero e um método de sua preparação compreendendo as seguintes etapas; cultura e colheita de células vero; disrupção e lise de células vero; purificação de proteínas de lise de células vero; e concentração para obter as proteínas de lise de células vero. as proteínas de lise de células vero preparadas de acordo com o método da presente invenção possuem uma ampla faixa de aplicações. elas podem ser empregadas no controle de qualidade e análise durante a preparação de vacinas usando as células vero, com alta sensibilidade e boa capacidade de repetição. a presente invenção também fornece um kit de teste de hcps de células vero, que possui forte especificidade, alta sensibilidade e boa capacidade de repetição. pode ser empregado no controle de qualidade e análise durante a preparação de não apenas vacinas virais não secretadas contra vírus tais como o vírus da hepatite a, mas também outras vacinas produzidas usando células vero.
BRPI0822510A 2008-04-30 2008-08-07 método de preparação e uso de proteínas de lise de células vero, e kit de ensaio para a determinação de hcps de células vero. BRPI0822510B8 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
CN200810043318.2 2008-04-30
CN2008100433197A CN101570566B (zh) 2008-04-30 2008-04-30 Vero细胞裂解蛋白、制备方法及其用途
CN200810043319.7 2008-04-30
CN2008100433182A CN101571549B (zh) 2008-04-30 2008-04-30 Vero细胞HCP检测试剂盒及其应用
PCT/CN2008/001434 WO2009132484A1 (zh) 2008-04-30 2008-08-07 Vero细胞裂解蛋白、其制备方法以及包含该蛋白的vero细胞hcp检测试剂盒

Publications (3)

Publication Number Publication Date
BRPI0822510A2 BRPI0822510A2 (pt) 2015-06-16
BRPI0822510B1 BRPI0822510B1 (pt) 2020-12-01
BRPI0822510B8 true BRPI0822510B8 (pt) 2021-05-25

Family

ID=41254761

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0822510A BRPI0822510B8 (pt) 2008-04-30 2008-08-07 método de preparação e uso de proteínas de lise de células vero, e kit de ensaio para a determinação de hcps de células vero.

Country Status (4)

Country Link
KR (1) KR101632635B1 (pt)
BR (1) BRPI0822510B8 (pt)
RU (1) RU2526131C2 (pt)
WO (1) WO2009132484A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107228894B (zh) * 2017-01-24 2019-03-19 浙江海隆生物科技有限公司 一种油乳剂蛋白亚单位疫苗中蛋白含量和纯度的检测方法及其应用
CN117192107A (zh) * 2023-09-11 2023-12-08 福建基诺厚普生物科技有限公司 一种工艺特异性宿主细胞蛋白残留的检测方法及试剂盒

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2042360C1 (ru) * 1992-07-06 1995-08-27 Биотехнологическая компания "Биосервис" Набор для выявления хламидийной инфекции
CN1843507B (zh) * 2006-02-14 2010-04-21 中国医学科学院医学生物学研究所 一种人用腮腺炎病毒组份疫苗及其制备方法和应用
SG170837A1 (en) * 2006-04-05 2011-05-30 Abbott Biotech Ltd Antibody purification
EP1878791A1 (en) * 2006-07-11 2008-01-16 Bia Separations D.O.O. Method for influenza virus purification

Also Published As

Publication number Publication date
BRPI0822510A2 (pt) 2015-06-16
BRPI0822510B1 (pt) 2020-12-01
RU2010143974A (ru) 2012-06-27
KR101632635B1 (ko) 2016-06-22
RU2526131C2 (ru) 2014-08-20
KR20110009210A (ko) 2011-01-27
WO2009132484A1 (zh) 2009-11-05

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Legal Events

Date Code Title Description
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B15K Others concerning applications: alteration of classification

Ipc: C12N 7/00 (2006.01), A61P 1/16 (2006.01)

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]

Free format text: NOTIFICACAO DE ANUENCIA RELACIONADA COM O ART 229 DA LPI

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 01/12/2020, OBSERVADAS AS CONDICOES LEGAIS.

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 07/08/2008 OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF

B25A Requested transfer of rights approved

Owner name: YUXI WALVAX BIOTECHNOLOGY CO., LTD. (CN)