BRPI0520520A2 - método simples de transplante de condrócitos injetáveis para o transplante autólogo de condrócitos - Google Patents
método simples de transplante de condrócitos injetáveis para o transplante autólogo de condrócitosInfo
- Publication number
- BRPI0520520A2 BRPI0520520A2 BRPI0520520-4A BRPI0520520A BRPI0520520A2 BR PI0520520 A2 BRPI0520520 A2 BR PI0520520A2 BR PI0520520 A BRPI0520520 A BR PI0520520A BR PI0520520 A2 BRPI0520520 A2 BR PI0520520A2
- Authority
- BR
- Brazil
- Prior art keywords
- mixing
- chondrocyte
- chondrocyte transplantation
- syringe
- injectable
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/36—Blood coagulation or fibrinolysis factors
- A61K38/363—Fibrinogen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/39—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/36—Blood coagulation or fibrinolysis factors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3817—Cartilage-forming cells, e.g. pre-chondrocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
- A61L27/3843—Connective tissue
- A61L27/3852—Cartilage, e.g. meniscus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Pharmacology & Pharmacy (AREA)
- Dermatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Cell Biology (AREA)
- Transplantation (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Hematology (AREA)
- Rheumatology (AREA)
- Urology & Nephrology (AREA)
- Vascular Medicine (AREA)
- Inorganic Chemistry (AREA)
- Materials For Medical Uses (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Prostheses (AREA)
Abstract
MéTODO SIMPLES DE TRANSPLANTE DE CONDRóCITOS INJETáVEIS PARA O TRANSPLANTE AUTOLOGO DE CONDRóCITOS. Que compreende a mistura de matrizes contendo fibrina, ácido hialurónico e colágeno, que constituem os principais ingredientes da cartilagem de origem animal, por meio do uso de uma ponta misturadora frontal de uma seringa, enquanto a mistura resultante é injetada em uma região cartilaginosa lesionada. Além disso, a presente invenção apresenta um método de transplante de condrócitos injetáveis para o transplante de condrócitos autólogos, que compreende as seguintes etapas: colocar 1 ml de uma cultura de condrócitos contida no Frasco No. 1 (Vermelho) em um seringa estéril de 1 ml e injetar a cultura celular no Frasco No. 2 (Azul) contendo pó liofilizado branco ou amarelo claro (fibrinogênio), por meio da inserção da seringa no Frasco No. 2, no sentido vertical, de tal modo que a concentração de fibrinogênio varie entre 10 mg/ml e 200 mg/ml; Injetar 1 ml da cultura celular contidas no Frasco No. 1 (Vermelho) no Frasco No. 3 (Vermelho, pequeno) contendo pó branco liofilizado (trombina), por meio da inserção da seringa no Frasco No. 3, na direção vertical, injetando, assim, gradualmente o liquido vermelho; Coletar 0,1 cc do conteúdo de 1 ml do Frasco No. 3 (Vermelho, pequeno), por meio do uso de uma seringa de 1 ml, e, em seguida, injetar no fundo do Frasco No. 4 (Amarelo), de tal modo que a concentração de trombina varie entre 1 UI/mI e 200 UI/mI; Adicionar todo o conteúdo de dois frascos contendo a suspensão de condrócitos ao Frasco No. 4 (Amarelo), por meio do uso de uma seringa estéril de 1 ml, e, em seguida, misturar o conteúdo (duas ou três vezes), utilizando a seringa; Confirmar a completa dissolução do conteúdo do Frasco No. 2 (Azul) e, em seguida, injetar todo o material dissolvido, em uma seringa de 1 ml; Injetar o agente terapêutico celular bem misturado do Frasco 4 (Amarelo) em uma seringa de 1 ml, e; Montar duas seringas de 1 ml cheias com o conteúdo do Frasco No. 2 (Azul) e do Frasco No. 4 (Amarelo) em um suporte fixo, e, em seguida, montar uma ponta misturadora frontal nas seringas e misturar os conteúdos das seringas, por meio do uso da ponta misturadora frontal, enquanto a mistura resultante a ser transplantada é injetada em uma região cartilaginosa lesionada de um animal. A presente invenção com a construção acima mencionada oferece efeitos vantajosos, como, por exemplo: solução para as inconveniências apresentadas pelo enxerto osteoperiosteal convencional, através da mistura e do transplante de matrizes contendo fibrina, ácido hialurónico e colágeno, que são os ingredientes principais da cartilagem; tratamento de uma grande variedade de defeitos de cartilagem e osteoartrite grave; alívio do sofrimento de pacientes a serem tratados, através de um método simplificado de operação cirúrgica, e; a conseqúente promoção da geração da cartilagem, de forma mais rápida e eficaz, o que resulta em melhor satisfação do paciente.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020050066026A KR100774089B1 (ko) | 2005-07-20 | 2005-07-20 | 주입형 연골세포치료제의 이식방법 |
PCT/KR2005/003147 WO2007011094A1 (en) | 2005-07-20 | 2005-09-23 | Simple method of transplanting injectable chondrocyte for autologous chondrocyte transplantation |
Publications (1)
Publication Number | Publication Date |
---|---|
BRPI0520520A2 true BRPI0520520A2 (pt) | 2009-05-12 |
Family
ID=37668959
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0520520-4A BRPI0520520A2 (pt) | 2005-07-20 | 2005-09-23 | método simples de transplante de condrócitos injetáveis para o transplante autólogo de condrócitos |
Country Status (18)
Country | Link |
---|---|
US (1) | US20090254065A1 (pt) |
EP (1) | EP1912661B1 (pt) |
JP (1) | JP2009501605A (pt) |
KR (1) | KR100774089B1 (pt) |
CN (1) | CN101247815A (pt) |
AT (1) | ATE501744T1 (pt) |
BR (1) | BRPI0520520A2 (pt) |
CY (1) | CY1111563T1 (pt) |
DE (1) | DE602005027004D1 (pt) |
DK (1) | DK1912661T3 (pt) |
ES (1) | ES2361420T3 (pt) |
HR (1) | HRP20110321T1 (pt) |
MX (1) | MX2008000787A (pt) |
PL (1) | PL1912661T3 (pt) |
PT (1) | PT1912661E (pt) |
RS (1) | RS51951B (pt) |
SI (1) | SI1912661T1 (pt) |
WO (1) | WO2007011094A1 (pt) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101114773B1 (ko) * | 2009-10-23 | 2012-03-05 | 세원셀론텍(주) | 연골조직 수복용 조성물의 제조방법 |
CN101716382B (zh) * | 2009-12-02 | 2015-07-15 | 浙江大学 | 一种质粒dna/纤维蛋白凝胶/聚合物三元复合支架的制备方法 |
KR20160129108A (ko) * | 2011-04-15 | 2016-11-08 | 가톨릭대학교 산학협력단 | 결합조직 재생용 조성물 및 결합조직을 재생하는 방법 |
KR101279812B1 (ko) * | 2012-05-16 | 2013-06-28 | 세원셀론텍(주) | 연골조직 수복용 조성물의 제조방법 |
CN105255814B (zh) * | 2015-10-27 | 2016-09-21 | 上海科医联创生物科技有限公司 | 一种用于微骨折手术的软骨诱导基质的制备方法 |
CN106581772B (zh) * | 2016-12-02 | 2020-02-18 | 四川大学华西医院 | 携带G-CSF缓释系统的Fibrin-HA水凝胶及其制备方法和用途 |
KR20190092059A (ko) * | 2018-01-30 | 2019-08-07 | 가톨릭대학교 산학협력단 | 연골세포, 피브리노겐, 콜라겐 또는 트롬빈을 포함하는 관절경하 수술을 위한 연골 재생용 조성물 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5842477A (en) * | 1996-02-21 | 1998-12-01 | Advanced Tissue Sciences, Inc. | Method for repairing cartilage |
US5866165A (en) * | 1997-01-15 | 1999-02-02 | Orquest, Inc. | Collagen-polysaccharide matrix for bone and cartilage repair |
US6886568B2 (en) * | 1998-04-08 | 2005-05-03 | The Johns Hopkins University | Method for fabricating cell-containing implants |
TR200200089T2 (tr) * | 1998-08-14 | 2002-06-21 | Verigen Transplantation Service International (Vtsi) Ag | Kondrosit hücre transplantasyonu için metodlar; enstrümanlar ve maddeler |
US20020082220A1 (en) * | 2000-06-29 | 2002-06-27 | Hoemann Caroline D. | Composition and method for the repair and regeneration of cartilage and other tissues |
US7931687B2 (en) * | 2002-05-13 | 2011-04-26 | Articular Engineering, Llc | Tissue engineered osteochondral implant |
US7316822B2 (en) * | 2003-11-26 | 2008-01-08 | Ethicon, Inc. | Conformable tissue repair implant capable of injection delivery |
KR100531922B1 (ko) | 2003-12-23 | 2005-11-29 | 주식회사 셀론텍 | 연골치료제 조성물 및 그 사용방법 |
-
2005
- 2005-07-20 KR KR1020050066026A patent/KR100774089B1/ko active IP Right Grant
- 2005-09-23 BR BRPI0520520-4A patent/BRPI0520520A2/pt not_active Application Discontinuation
- 2005-09-23 ES ES05809011T patent/ES2361420T3/es active Active
- 2005-09-23 WO PCT/KR2005/003147 patent/WO2007011094A1/en active Application Filing
- 2005-09-23 EP EP05809011A patent/EP1912661B1/en active Active
- 2005-09-23 CN CNA2005800510893A patent/CN101247815A/zh active Pending
- 2005-09-23 JP JP2008522686A patent/JP2009501605A/ja active Pending
- 2005-09-23 AT AT05809011T patent/ATE501744T1/de active
- 2005-09-23 PL PL05809011T patent/PL1912661T3/pl unknown
- 2005-09-23 RS RS20110196A patent/RS51951B/en unknown
- 2005-09-23 SI SI200531297T patent/SI1912661T1/sl unknown
- 2005-09-23 MX MX2008000787A patent/MX2008000787A/es active IP Right Grant
- 2005-09-23 DE DE602005027004T patent/DE602005027004D1/de active Active
- 2005-09-23 PT PT05809011T patent/PT1912661E/pt unknown
- 2005-09-23 US US11/988,924 patent/US20090254065A1/en not_active Abandoned
- 2005-09-23 DK DK05809011.9T patent/DK1912661T3/da active
-
2011
- 2011-05-03 HR HR20110321T patent/HRP20110321T1/hr unknown
- 2011-06-15 CY CY20111100569T patent/CY1111563T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
DK1912661T3 (da) | 2011-06-06 |
PL1912661T3 (pl) | 2011-08-31 |
ATE501744T1 (de) | 2011-04-15 |
PT1912661E (pt) | 2011-05-25 |
KR20070010990A (ko) | 2007-01-24 |
CN101247815A (zh) | 2008-08-20 |
WO2007011094A1 (en) | 2007-01-25 |
SI1912661T1 (sl) | 2011-07-29 |
US20090254065A1 (en) | 2009-10-08 |
RS51951B (en) | 2012-02-29 |
EP1912661A4 (en) | 2009-09-09 |
EP1912661A1 (en) | 2008-04-23 |
JP2009501605A (ja) | 2009-01-22 |
CY1111563T1 (el) | 2015-10-07 |
HRP20110321T1 (hr) | 2011-06-30 |
MX2008000787A (es) | 2008-04-08 |
ES2361420T3 (es) | 2011-06-16 |
KR100774089B1 (ko) | 2007-11-06 |
DE602005027004D1 (de) | 2011-04-28 |
EP1912661B1 (en) | 2011-03-16 |
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Legal Events
Date | Code | Title | Description |
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B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] | ||
B07E | Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
B09B | Patent application refused [chapter 9.2 patent gazette] | ||
B09B | Patent application refused [chapter 9.2 patent gazette] |
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