MY144022A - Simple method of transplanting injectable chondrocyte for autologous chondrocyte transplantation - Google Patents

Abstract

THE PRESENT INVENTION RELATES TO A METHOD OF TRANSPLANTING AN INJECTABLE CHONDROCYTE FOR AUTOLOGOUS CHONDROCYTE TRANSPLANTATION. FOR THIS PURPOSE, THE PRESENT INVENTION PROVIDES A METHOD OF TRANSPLANTING AN INJECTABLE CHONDROCYTE FOR AUTOLOGOUS CHONDROCYTE TRANSPLANTATION, COMPRISING MIXING MATRICES INCLUDING FIBRIN, HYALURONIC ACID AND COLLAGEN, CONSTITUTING MAIN INGREDIENTS OF ANIMAL CARTILAGE, VIA USE OF A FRONT END MIXING TIP OF A SYRINGE, WHILE INJECTING THE RESULTING MIXTURE INTO A DAMAGED CARTILAGE REGION. FURTHER, THE PRESENT INVENTION PROVIDES A METHOD OF TRANSPLANTING AN INJECTABLE CHONDROCYTE FOR AUTOLOGOUS CHONDROCYTE TRANSPLANTATION, COMPRISING PLACING 1 ML OF A CHONDROCYTE CULTURE CONTAINED IN VIAL NO. 1 (RED) INTO A 1 ML STERILE SYRINGE AND INJECTING THE CELL CULTURE INTO VIAL NO. 2 (BLUE) CONTAINING WHITE OR LIGHT-YELLOW LYOPHILIZED POWDER (FIBRINOGEN) BY INSERTING THE SYRINGE INTO VIAL NO. 2 IN THE VERTICAL DIRECTION, SUCH THAT THE CONCENTRATION OF FIBRINOGEN IS IN THE RANGE OF 10 MG/ML TO 200 MG/ML; INJECTING 1 ML OF CELL CULTURE CONTAINED IN VIAL NO. 1 (RED) INTO VIAL NO. 3 (RED, SMALL) CONTAINING WHITE LYOPHILIZED POWDER (THROMBIN) BY INSERTING THE SYRINGE INTO VIAL NO. 3 IN THE VERTICAL DIRECTION, THEREBY GRADUALLY INJECTING RED LIQUID; COLLECTING 0.1 CC OF 1 ML CONTENT OF VIAL NO. 3 (RED, SMALL) USING A 1 ML SYRINGE, FOLLOWED BY INJECTION INTO A BOTTOM OF VIAL NO.4 (YELLOW) SUCH THAT THE CONCENTRATION OF THROMBIN IS IN THE RANGE OF 1 IU/ML TO 200 IU/ML; ADDING THE ENTIRE CONTENTS OF TWO VIALS CONTAINING CHONDROCYTE SUSPENSION TO VIAL NO.4 (YELLOW) USING A 1ML STERILE SYRINGE, FOLLOWED BY MIXING THE CONTENTS TWO OR THREE TIMES USING THE SYRINGE; CONFIRMING COMPLETE DISSOLUTION OF THE CONTENTS OF VIAL NO.2 (BLUE) AND THEN INJECTING THE ENTIRETY OF THE DISSOLVED MATERIAL INTO A 1 ML SYRINGE; INJECTING THE WELL-MIXED CELL THERAPEUTIC AGENT OF VIAL 4 (YELLOW) INTO A 1 ML SYRINGE; AND MOUNTING TWO 1 ML SYRINGES FILLED WITH THE CONTENTS OF VIAL NO.2 (BLUE) AND VIAL NO.4 (YELLOW) ON A STANDING HOLDER FOLLOWED BY MOUNTING A SCREW TIP ON THE SYRINGES, AND MIXING THE CONTENTS OF THE SYRINGES USING FRONT END MIXING TIP THEREOF WHILE INJECTING THE RESULTING MIXTURE TO BE TRANSPLANTED INTO A DAMAGED CARTILAGE REGION OF AN ANIMAL. THE PRESENT INVENTION HAVING THE ABOVE-MENTIONED CONSTRUCTION PROVIDES ADVANTAGEOUS EFFECTS SUCH AS SOLUTION OF INCONVENIENCE EXHIBITED BY CONVENTIONAL OSTEOPERIOSTEAL GRAFTING, BY MIXING AND TRANSPLANTING MATRICES INCLUDING FIBRIN, HYALURONIC ACID AND COLLAGEN WHICH ARE MAIN INGREDIENTS OF CARTILAGE; TREATMENT OF A WIDE VARIETY OF CARTILAGE DEFECTS AND SEVERE OSTEOARTHRITIS; ALLEVIATION OF BURDEN OF PATIENTS TO BE TREATED VIA A SIMPLIFIED SURGICAL OPERATION METHOD; AND THEREBY PROMOTION OF RAPIDER AND MORE EFFECTIVE CARTILAGE GENERATION, RESULTING IN IMPROVED CUSTOMER SATISFACTION.