BRPI0314543B8 - células hospedeiras, dna recombinante, utilização dos mesmos, processos de produção de um polipeptídeo implicado na biossíntese das espiramicinas, de produção de macrolídeos e de produção de espiramicina(s), e micro-organismo transgênico mutante produtor de macrolídeo - Google Patents

células hospedeiras, dna recombinante, utilização dos mesmos, processos de produção de um polipeptídeo implicado na biossíntese das espiramicinas, de produção de macrolídeos e de produção de espiramicina(s), e micro-organismo transgênico mutante produtor de macrolídeo

Info

Publication number
BRPI0314543B8
BRPI0314543B8 BRPI0314543A BR0314543A BRPI0314543B8 BR PI0314543 B8 BRPI0314543 B8 BR PI0314543B8 BR PI0314543 A BRPI0314543 A BR PI0314543A BR 0314543 A BR0314543 A BR 0314543A BR PI0314543 B8 BRPI0314543 B8 BR PI0314543B8
Authority
BR
Brazil
Prior art keywords
production
spiramycin
biosynthesis
spiramycins
genes
Prior art date
Application number
BRPI0314543A
Other languages
English (en)
Other versions
BR0314543A (pt
BRPI0314543B1 (pt
Inventor
Gondran Anne
Gerbaud Claude
Darbon-Rongere Emmanuelle
Karray Fatma
Dominguez Hélène
Pernodet Jean-Luc
Tuphile Karine
Blondelet-Rouault Marie-Hélène
Oestreicher-Mermet-Bouvier Nathalie
Lacroix Patricia
Original Assignee
Aventis Pharma Sa
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR0212489A external-priority patent/FR2845394A1/fr
Priority claimed from FR0302439A external-priority patent/FR2851773A1/fr
Application filed by Aventis Pharma Sa, Centre Nat Rech Scient filed Critical Aventis Pharma Sa
Publication of BR0314543A publication Critical patent/BR0314543A/pt
Publication of BRPI0314543B1 publication Critical patent/BRPI0314543B1/pt
Publication of BRPI0314543B8 publication Critical patent/BRPI0314543B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

"polipeptídeos implicados na biossíntese das espiramicinas, seqüências nucleotídicas codificando esses polipeptídeos e suas aplicações". a presente invenção refere-se ao isolamento e à identificação de novos genes da via de biossíntese das espiramicinas e de novos polipeptídeos implicados nessa biossíntese. a invenção é também relativa a um processo de produção desses polipeptídeos. ela é também relativa à utilização desses genes com a finalidade de aumentar as taxas de produção e a pureza da espiramicina produzida. a invenção refere-se notadamente a um microorganismo que produz a espiramicina i, mas que não produz a espiramicina ii e iii e a utilização desse microorganismo. a invenção refere-se também à utilização dos genes da via de biossíntese das espiramicinas para a construção de mutantes que podem levar à síntese de novos antibióticos ou a formas derivadas de espiramicinas. a invenção refere-se ainda às moléculas produzidas graças à expressão desses genes e das composições farmacologicamente ativas de uma molécula produzida graças à expressão desses genes.
BRPI0314543A 2002-10-08 2003-10-08 células hospedeiras, dna recombinante, utilização dos mesmos, processos de produção de um polipeptídeo implicado na biossíntese das espiramicinas, de produção de macrolídeos e de produção de espiramicina(s), e micro-organismo transgênico mutante produtor de macrolídeo BRPI0314543B8 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
FR0212489A FR2845394A1 (fr) 2002-10-08 2002-10-08 Polypeptides impliques dans la biosynthese des spiramycines, sequences nucleotidiques codant ces polypeptides et leurs applications
FR0302439A FR2851773A1 (fr) 2003-02-27 2003-02-27 Polypeptides impliques dans la biosynthese des spiramycines, sequences nucleotidiques codant ces polypeptides et leurs applications
US49349003P 2003-08-07 2003-08-07
PCT/FR2003/002962 WO2004033689A2 (fr) 2002-10-08 2003-10-08 Polypeptides impliques dans la biosynthese des spiramycines, sequences nucleotidiques codant ces polypeptides et leurs applications.

Publications (3)

Publication Number Publication Date
BR0314543A BR0314543A (pt) 2005-08-09
BRPI0314543B1 BRPI0314543B1 (pt) 2016-06-14
BRPI0314543B8 true BRPI0314543B8 (pt) 2021-05-25

Family

ID=32096598

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0314543A BRPI0314543B8 (pt) 2002-10-08 2003-10-08 células hospedeiras, dna recombinante, utilização dos mesmos, processos de produção de um polipeptídeo implicado na biossíntese das espiramicinas, de produção de macrolídeos e de produção de espiramicina(s), e micro-organismo transgênico mutante produtor de macrolídeo

Country Status (10)

Country Link
EP (1) EP1551975A2 (pt)
JP (1) JP5042497B2 (pt)
KR (1) KR101110175B1 (pt)
AU (1) AU2003300479B2 (pt)
BR (1) BRPI0314543B8 (pt)
CA (1) CA2501445C (pt)
IL (1) IL167854A (pt)
MX (1) MXPA05002852A (pt)
NO (1) NO335995B1 (pt)
WO (1) WO2004033689A2 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111349595A (zh) * 2018-12-20 2020-06-30 沈阳福洋医药科技有限公司 一种螺旋霉素产生菌、可利霉素产生菌、构建方法、应用及提高产物产量的方法
WO2024074640A1 (en) * 2022-10-07 2024-04-11 Syngenta Crop Protection Ag Fungicidal mixture comprising streptimidone and malonomicin

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5068189A (en) * 1988-05-13 1991-11-26 Eli Lilly And Company Recombinant dna vectors encoding a 4"-o-isovaleryl acylase derived from a carbomycin biosynthetic gene, designated care, for use in streptomyces and other organisms
JP2749616B2 (ja) * 1988-05-24 1998-05-13 メルシャン株式会社 マクロライド抗生物質の４”位アシル化酵素をコードする遺伝子
US5098837A (en) * 1988-06-07 1992-03-24 Eli Lilly And Company Macrolide biosynthetic genes for use in streptomyces and other organisms
US5322937A (en) * 1990-06-01 1994-06-21 Mercian Corporation Genes encoding a 3-acylation enzyme for macrolide antibiotics
US5514544A (en) * 1991-07-26 1996-05-07 Eli Lilly And Company Activator gene for macrolide biosynthesis
JPH0638750A (ja) * 1991-08-09 1994-02-15 Meiji Seika Kaisha Ltd マクロライド抗生物質の３位アシル化酵素およびそれをコードする遺伝子
JPH06121677A (ja) * 1992-01-23 1994-05-06 Mercian Corp マクロライド抗生物質のアシル化酵素遺伝子の高発現方法
CA2197160C (en) * 1996-02-22 2007-05-01 Stanley Gene Burgett Platenolide synthase gene
CA2197524A1 (en) * 1996-02-22 1997-08-22 Bradley Stuart Dehoff Polyketide synthase genes
JP2001511349A (ja) * 1997-07-25 2001-08-14 ヘキスト・マリオン・ルセル Ｓａｃｃｈａｒｏｐｏｌｙｓｐｏｒａｅｒｙｔｈｒａｅａ及びＳｔｒｅｐｔｏｍｙｃｅｓａｎｔｉｂｉｏｔｉｃｕｓにおける６−デオキシヘキソースの生合成及び移送のための遺伝子
EP1409686B1 (en) * 2001-07-26 2006-03-22 Ecopia Biosciences Inc. Genes and proteins for the biosynthesis of rosaramicin

Also Published As

Publication number Publication date
JP2006501863A (ja) 2006-01-19
BR0314543A (pt) 2005-08-09
KR20050084843A (ko) 2005-08-29
AU2003300479A1 (en) 2004-05-04
IL167854A (en) 2011-06-30
NO335995B1 (no) 2015-04-13
MXPA05002852A (es) 2005-10-05
NO20052160L (no) 2005-06-02
CA2501445A1 (fr) 2004-04-22
BRPI0314543B1 (pt) 2016-06-14
AU2003300479B2 (en) 2009-12-10
KR101110175B1 (ko) 2012-07-18
EP1551975A2 (fr) 2005-07-13
CA2501445C (fr) 2016-01-26
JP5042497B2 (ja) 2012-10-03
WO2004033689A3 (fr) 2004-08-26
WO2004033689A2 (fr) 2004-04-22

Similar Documents

Publication Publication Date Title
Yasukawa et al. Defect in modification at the anticodon wobble nucleotide of mitochondrial tRNALys with the MERRF encephalomyopathy pathogenic mutation
BR0115179A (pt) polinucletìdeos isolados, vetor, construção de dna recombinante, método de transformação de uma célula, célula, método de produção de uma planta, planta, semente, polipeptìdeo isolado e método para isolamento de um polipeptìdeo
Malekan et al. One-pot multi-enzyme (OPME) chemoenzymatic synthesis of sialyl-Tn-MUC1 and sialyl-T-MUC1 glycopeptides containing natural or non-natural sialic acid
JPWO2004101619A1 (ja) 機能的糖ペプチドの合理的設計および合成
BR0112979A (pt) Método de produzir domìnios de proteìna funcional
BR0011500A (pt) Hialuronidase de hirudinaria manillensis, isolamento, purificação e método recombinante de produção
US7393670B2 (en) Tyrosyl-tRNA synthetase variants
NO20034692L (no) Nye polynukleotider og polypeptider av INF gamma-17 genet
BR9911349A (pt) Genes para a biossìntese de epotilonas
BR0214489A (pt) Processo para a produção de um polipeptìdeo recombinante
Azevedo et al. The new world of inorganic polyphosphates
Nameki et al. Recognition of tRNAGly by three widely diverged glycyl-tRNA synthetases
BRPI0314543B8 (pt) células hospedeiras, dna recombinante, utilização dos mesmos, processos de produção de um polipeptídeo implicado na biossíntese das espiramicinas, de produção de macrolídeos e de produção de espiramicina(s), e micro-organismo transgênico mutante produtor de macrolídeo
Liu et al. GBPI, a novel gastrointestinal-and brain-specific PP1-inhibitory protein, is activated by PKC and inactivated by PKA
EP3933044B1 (en) Practical enzymatic synthesis of 3',3'-cgamp
Wang et al. Probing the roles of conserved residues in uridyltransferase domain of Escherichia coli K12 GlmU by site-directed mutagenesis
Prijatelj et al. Charge reversal of ammodytoxin A, a phospholipase A2-toxin, does not abolish its neurotoxicity
WO2003000896A3 (en) POLYNUCLEOTIDES AND POLYPEPTIDES OF THE IFNα-5 GENE
Benito et al. The structural determinants that lead to the formation of particular oligomeric structures in the pancreatic-type ribonuclease family
BR0109258A (pt) Enzimas envolvidas em sìntese de triterpeno
BRPI0013119B8 (pt) molécula polinucleotídica isolada, vetor recombinante purificado, célula hospedeira procariótica e célula de streptomyces avermitilis mutada, método para obtenção de uma nova cepa de s. avermitilis e processo para produção de avermectinas
WO2002101048A3 (en) NEW POLYNUCLEOTIDES AND POLYPEPTIDES OF THE IFNα-7 GENE
Li et al. Xylosyltransferase Bump-and-hole Engineering to Chemically Manipulate Proteoglycans in Mammalian Cells
Scholz et al. Variation of the intercalating proline in artificial peptides mimicking the DNA binding and bending IHF protein
Knoll et al. Mapping of the active site of T7 RNA polymerase with 8-azidoATP

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: AS CLASSIFICACOES ANTERIORES ERAM: C12N 15/52 , C12N 9/00 , C12N 15/63 , C12N 1/21 , C07K 14/36 , C12P 19/62

Ipc: C12N 15/52 (2006.01), C07K 14/36 (2006.01), C12P 1

B07E Notice of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]

Free format text: NOTIFICACAO DE ANUENCIA RELACIONADA COM O ART 229 DA LPI

B07A Technical examination (opinion): publication of technical examination (opinion) [chapter 7.1 patent gazette]
B07A Technical examination (opinion): publication of technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 14/06/2016, OBSERVADAS AS CONDICOES LEGAIS.

B16C Correction of notification of the grant

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 08/10/2003 OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF