BR9811804A - Plantas transgênicas com tocoferol metiltransferase - Google Patents

Plantas transgênicas com tocoferol metiltransferase

Info

Publication number
BR9811804A
BR9811804A BR9811804-8A BR9811804A BR9811804A BR 9811804 A BR9811804 A BR 9811804A BR 9811804 A BR9811804 A BR 9811804A BR 9811804 A BR9811804 A BR 9811804A
Authority
BR
Brazil
Prior art keywords
tocopherol
gamma
transgenic plants
methyltransferase
plant
Prior art date
Application number
BR9811804-8A
Other languages
English (en)
Inventor
Dean Dellapenna
David K Shintani
Original Assignee
Univ Nevada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Nevada filed Critical Univ Nevada
Publication of BR9811804A publication Critical patent/BR9811804A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Fats And Perfumes (AREA)

Abstract

"PLANTAS TRANSGêNICAS COM TOCOFEROL METILTRANSFERASE" São descritas seq³ências de genes que codificam gama-tocoferol metiltransferases a partir de organismos fotossintéticos. A enzima gama-tocoferol metiltransferase catalisa a metilação de gama-tocoferol para produzir alfa-tocoferol, que é a espécie de tocoferol mais bioativa. Acredita-se que gama-tocoferol metiltransferase esteja envolvida em regular as quantidades relativas dos vários tocoferóis presentes em organismos fotossintéticos. Ao introduzir uma construção genética possuindo uma seq³ência que codifica a gama-tocoferol metiltransferase colocada sob o controle de um promotor de planta em uma planta, plantas transgênicas podem ser feitas possuindo expressão gama-tocoferol metiltransferase alterada, para obter mudanças dramáticas no perfil de tocoferol da planta. Plantas transgênicas podem ser produzidas possuindo alfa-tocoferol como o tocoferol predominante em suas sementes e óleos.
BR9811804-8A 1997-07-25 1998-07-22 Plantas transgênicas com tocoferol metiltransferase BR9811804A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5381997P 1997-07-25 1997-07-25
US7249798P 1998-01-26 1998-01-26
PCT/US1998/015137 WO1999004622A1 (en) 1997-07-25 1998-07-22 Transgenic plants with tocopherol methyltransferase

Publications (1)

Publication Number Publication Date
BR9811804A true BR9811804A (pt) 2001-09-11

Family

ID=26732278

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9811804-8A BR9811804A (pt) 1997-07-25 1998-07-22 Plantas transgênicas com tocoferol metiltransferase

Country Status (7)

Country Link
US (3) US6642434B1 (pt)
EP (1) EP1009812A4 (pt)
JP (1) JP2004514401A (pt)
AU (1) AU8506198A (pt)
BR (1) BR9811804A (pt)
CA (1) CA2298050A1 (pt)
WO (1) WO1999004622A1 (pt)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6642434B1 (en) * 1997-07-25 2003-11-04 University Of Community College System Of Nevada Transgenic plants with γ-tocopherol methyltransferase
US7071381B1 (en) 1998-12-03 2006-07-04 E. I. Du Pont De Nemours & Company Plant vitamin e biosynthetic enzymes
WO2000032757A2 (en) * 1998-12-03 2000-06-08 E.I. Du Pont De Nemours And Company Plant vitamin e biosynthetic enzymes
ATE446370T1 (de) 1999-04-12 2009-11-15 Monsanto Technology Llc Öl, das brassicastanol enthält
ATE316578T1 (de) 1999-04-15 2006-02-15 Calgene Llc Nukleinsäuresequenzen für proteine, die an der tocopherolbiosynthese beteiligt sind
US6787683B1 (en) 1999-05-07 2004-09-07 Pioneer Hi-Bred International, Inc. Phytyl/prenyltransferase nucleic acids, polypeptides and uses thereof
DE19931834A1 (de) * 1999-07-09 2001-01-11 Sungene Gmbh & Co Kgaa Identifizierung und Überexpression einer DNA-Sequenz codierend für eine 2-Methyl-6-phytylhydrochinon-methyltransferase in Pflanzen
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
DE60129011T2 (de) * 2000-04-14 2008-02-28 Calgene Llc, Davis Nukleinsäuresequenzen für proteine, die an der tocopherolbiosynthese beteiligt sind
AR030430A1 (es) 2000-06-29 2003-08-20 Sungene Gmbh & Co Kgaa Procedimiento para la obtencion de quimicos finos por cultivo de organismos que presentan una via de shiquimato modificada, composicion de acido nucleinico, uso de dicho acido nucleinico para la obtencion de plantas transgenicas, organismo geneticamente modificado, procedimiento para la produccion d
CA2418436C (en) 2000-08-07 2017-07-11 Monsanto Technology, Llc Methyl-d-erythritol phosphate pathway genes
DE10046462A1 (de) * 2000-09-19 2002-05-29 Sungene Gmbh & Co Kgaa Verbesserte Verfahren zur Vitamin E Biosynthese
US6822142B2 (en) 2001-01-05 2004-11-23 Monsanto Company Transgenic plants containing altered levels of steroid compounds
WO2002063022A2 (en) * 2001-02-08 2002-08-15 Monsanto Technology Llc Plant regulatory sequences
DE10111676A1 (de) 2001-03-09 2002-09-12 Sungene Gmbh & Co Kgaa Erhöhung des Vitamin-E-Gehalts in Organismen durch Erhöhung der Tyrosinaminotransferase-Aktivität
US6977269B1 (en) 2001-05-03 2005-12-20 Pioneer Hi-Bred International, Inc Method of improving animal tissue quality
US7161061B2 (en) * 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
EP1392106B1 (en) 2001-05-09 2008-12-31 Monsanto Technology LLC Tyra genes and uses thereof
US7244877B2 (en) * 2001-08-17 2007-07-17 Monsanto Technology Llc Methyltransferase from cotton and uses thereof
EP1897952A3 (en) * 2001-08-17 2009-12-09 Monsanto Technology, LLC Methyltransferasegenes and uses thereof
AU2002342114B2 (en) 2001-10-25 2008-07-31 Monsanto Technology Llc Aromatic methyltransferases and uses thereof
BR0308740A (pt) 2002-03-19 2007-01-09 Monsanto Technology Llc ácidos nucléicos e polipeptìdeos de homogentisado prenil transferase ("hpt"), e empregos destes
KR20030080931A (ko) * 2002-04-11 2003-10-17 윤성중 식물체 내에서 α-토코페롤의 생산을 증진시키는 방법
BR0313270A (pt) 2002-08-05 2005-08-02 Monsanto Technology Llc Genes relacionados com a biosìntese de tocoferol e usos dos mesmos
DE10260871A1 (de) * 2002-12-23 2004-07-08 Sungene Gmbh & Co. Kgaa Verfahren zur Herstellung von transgenen Pflanzen mit erhöhtem Vitamin E-Gehalt durch Veränderung des Serin-Acetyltransferase-Gehalts
US20070199096A1 (en) 2005-11-14 2007-08-23 E.I. Du Pont De Nemours And Company Compositions and Methods for Altering Alpha- and Beta-Tocotrienol Content
KR100908567B1 (ko) * 2006-09-25 2009-07-21 보령제약 주식회사 식물 세포배양에서 아미노산과 피루브산을 이용한 목적단백질의 생산 방법
WO2008038932A1 (en) * 2006-09-25 2008-04-03 Boryung Pharmaceutical Co., Ltd Method for producing target proteins using amino acids and pyruvic acids in culture of plant cells
US7893324B2 (en) 2007-10-04 2011-02-22 E.I. Dupont De Nemours And Company Compositions and methods for altering alpha- and beta-tocotrienol content using multiple transgenes
CN102839199B (zh) * 2012-08-16 2014-11-05 熊军 一种酶工程法生产d-α-生育酚的方法
CN113817705B (zh) * 2021-10-27 2023-03-24 陕西海斯夫生物工程有限公司 一种γ-生育酚甲基转移酶突变体及其在提高α-生育酚及α-生育三烯酚产量的应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6429356B1 (en) * 1996-08-09 2002-08-06 Calgene Llc Methods for producing carotenoid compounds, and specialty oils in plant seeds
US6642434B1 (en) 1997-07-25 2003-11-04 University Of Community College System Of Nevada Transgenic plants with γ-tocopherol methyltransferase
US6200395B1 (en) 1997-11-17 2001-03-13 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Free-machining steels containing tin antimony and/or arsenic
EP1107662A4 (en) 1998-08-25 2003-07-02 Univ Nevada MANIPULATION OF TOCOPHEROL RATES IN TRANSGENIC PLANTS
WO2000032757A2 (en) 1998-12-03 2000-06-08 E.I. Du Pont De Nemours And Company Plant vitamin e biosynthetic enzymes
BR0114847A (pt) 2000-10-14 2005-01-11 Calgene Llc Sequências de ácido nucléico para proteìnas envolvidas em sìntese de tocoferol
US7244877B2 (en) 2001-08-17 2007-07-17 Monsanto Technology Llc Methyltransferase from cotton and uses thereof
WO2003089452A2 (en) 2002-04-17 2003-10-30 Sangamo Biosciences, Inc. Compositions and methods for regulation of plant gamma-tocopherol methyltransferase
US20040091990A1 (en) 2002-08-29 2004-05-13 Guofu Li Compositions and methods for regulation of plant gamma-tocopherol methyltransferase

Also Published As

Publication number Publication date
JP2004514401A (ja) 2004-05-20
US7572952B2 (en) 2009-08-11
US20100017917A1 (en) 2010-01-21
WO1999004622A1 (en) 1999-02-04
CA2298050A1 (en) 1999-02-04
EP1009812A1 (en) 2000-06-21
US6642434B1 (en) 2003-11-04
US20050251875A1 (en) 2005-11-10
AU8506198A (en) 1999-02-16
EP1009812A4 (en) 2002-01-09

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Legal Events

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B08F Application fees: application dismissed [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1975 DE 11/11/2008.