BR9807308A - Método de seleção para plantas transgênicas - Google Patents

Método de seleção para plantas transgênicas

Info

Publication number
BR9807308A
BR9807308A BR9807308-7A BR9807308A BR9807308A BR 9807308 A BR9807308 A BR 9807308A BR 9807308 A BR9807308 A BR 9807308A BR 9807308 A BR9807308 A BR 9807308A
Authority
BR
Brazil
Prior art keywords
transformed cells
component
medium
cells
metabolic derivative
Prior art date
Application number
BR9807308-7A
Other languages
English (en)
Inventor
Iain Alasdair Donaldson
Kirten Jorgensen
Morten Jorsboe
Kirsten Bojsen
Original Assignee
Danisco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco filed Critical Danisco
Publication of BR9807308A publication Critical patent/BR9807308A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Hydroponics (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Patente de Invenção <B>"MéTODO DE SELEçãO PARA PLANTAS TRANSGêNICAS"<D>. Um método de seleção para selecionar de uma população de células, uma ou mais células transformadas geneticamente selecionáveis é descrito. No método, a população de células compreende células transformadas geneticamente selecionáveis e possíveis células não transformadas. Cada uma das células transformadas geneticamente selecionáveis compreende uma primeira seq³ência de nucleotídeos expressáveis e, opcionalmente, uma segunda seq³ência de nucleotídeos expressáveis. No método, um componente ou um derivado metabólico dele, quando presente em uma baixa concentração em um meio, é um nutriente para ambas as células transformadas geneticamente selecionáveis e as células não transformadas. No método, o componente ou o derivado metabólico dele, quando presente em uma alta concentração em um meio, é tóxico para as células não transformadas. A primeira seq³ência de nucleotídeos codifica um produto de gene, capaz de converter o componente ou o derivado metabólico dele, quando presente em uma alta concentração em um meio, em um nutriente para as células transformadas geneticamente selecionáveis. O método compreende a etapa de introdução da população de célula em um meio, em que o meio compreende, opcionalmente, uma alta concentração do componente ou do seu derivado metabólico. No método, o componente ou seu derivado metabólico é uma fonte tanto de carboidratos e de nitrogênio para as células transformadas geneticamente selecionáveis. Alternativamente, no método, se uma parte do componente serve como um substrato metabólico e é metabolicamente convertido em um substrato derivado, então esse substrato derivado é capaz de proporcionar um efeito alostérico no produto de gene.
BR9807308-7A 1997-02-07 1998-02-05 Método de seleção para plantas transgênicas BR9807308A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9702592.8A GB9702592D0 (en) 1997-02-07 1997-02-07 Selection method
PCT/GB1998/000367 WO1998035047A1 (en) 1997-02-07 1998-02-05 Selection method for transgenic plants

Publications (1)

Publication Number Publication Date
BR9807308A true BR9807308A (pt) 2000-05-02

Family

ID=10807294

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9807308-7A BR9807308A (pt) 1997-02-07 1998-02-05 Método de seleção para plantas transgênicas

Country Status (11)

Country Link
US (1) US6444878B1 (pt)
EP (1) EP0970221A1 (pt)
JP (1) JP2002514915A (pt)
KR (1) KR20000070886A (pt)
CN (1) CN1252097A (pt)
AU (1) AU739067B2 (pt)
BR (1) BR9807308A (pt)
CA (1) CA2280236A1 (pt)
GB (2) GB9702592D0 (pt)
NZ (1) NZ336925A (pt)
WO (1) WO1998035047A1 (pt)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9817465D0 (en) * 1998-08-11 1998-10-07 Danisco Selection method
EP1546352A2 (en) * 2002-09-30 2005-06-29 Archer-Daniels-Midland Company Cell-free production of glucosamine
EP1772052A1 (de) 2005-10-05 2007-04-11 Bayer CropScience GmbH Verbesserte Verfahren und Mittel zur Herstellung von Hyaluronan
CA2624338C (en) 2005-10-05 2016-09-13 Bayer Cropscience Ag Plants having an increased content of amino sugars
EP2036983A1 (de) 2007-09-12 2009-03-18 Bayer CropScience AG Pflanzen, die erhöhte Mengen an Glucosaminglycanen synthetisieren
WO2010102293A1 (en) 2009-03-06 2010-09-10 Metabolix, Inc. Method of positive plant selection using sorbitol dehydrogenase
BR112012005592A2 (pt) 2009-09-15 2017-05-02 Donald Danforth Plant Science Center geração de sementes oleaginosas produzindo alto poliidroxibutirato.
ES2461896B1 (es) 2010-12-21 2015-03-18 Centro De Investigación Y De Estudios Avanzados Del Instituto Politécnico Nacional Métodos para obtener plantas resistentes a sequía
US20140182011A1 (en) 2011-11-03 2014-06-26 The University Of Hong Kong Methods Using Acyl-Coenzyme A-Binding Proteins to Enchance Drought Tolerance in Genetically Modified Plants
WO2013184768A1 (en) 2012-06-05 2013-12-12 University Of Georgia Research Foundation, Inc. Compositions and methods of gene silencing in plants
US20150211019A1 (en) 2012-08-13 2015-07-30 University Of Georgia Research Foundation, Inc. Compositions and Methods for Increasing Pest Resistance in Plants
CN103651078B (zh) * 2013-12-12 2015-10-07 浙江师范大学 一种筛选转基因植物种子的方法
WO2016164810A1 (en) 2015-04-08 2016-10-13 Metabolix, Inc. Plants with enhanced yield and methods of construction
US20190338302A1 (en) 2016-02-04 2019-11-07 Yield10 Bioscience, Inc. Transgenic land plants comprising a putative transporter protein of an edible eukaryotic algae
CA3054060C (en) 2017-02-22 2023-09-26 Yield10 Bioscience, Inc. Transgenic land plants comprising enhanced levels of mitochondrial transporter protein
EP3846615A4 (en) 2018-09-04 2022-05-25 Yield10 Bioscience, Inc. GENETICALLY MODIFIED LAND PLANTS THAT EXPRESS AN INCREASED SEED YIELD PROTEIN AND/OR AN RNA FOR INCREASED SEED YIELD
CN109593770A (zh) * 2018-12-26 2019-04-09 江苏省农业科学院 一种不在原核生物中表达的抗生素类转基因植物标记基因的获得方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9304200D0 (en) * 1993-03-02 1993-04-21 Sandoz Ltd Improvements in or relating to organic compounds
DK152291D0 (da) * 1991-08-28 1991-08-28 Danisco Fremgangsmaade og kemiske forbindelser
DK169215B1 (da) 1991-09-02 1994-09-12 Peter Dalgaard Indretning til hindring af fingerklemning i døre
AU2570992A (en) * 1991-09-20 1993-04-27 Mogen International N.V. Tryptophan analogues as selective agents in the transformation of plants and plant cells
MX9707666A (es) * 1995-04-06 1997-11-29 Seminis Vegetables Proceso de seleccion de celulas de planta transgenica.

Also Published As

Publication number Publication date
US6444878B1 (en) 2002-09-03
EP0970221A1 (en) 2000-01-12
KR20000070886A (ko) 2000-11-25
AU5997298A (en) 1998-08-26
GB9702592D0 (en) 1997-03-26
GB2341863A (en) 2000-03-29
NZ336925A (en) 2001-10-26
CA2280236A1 (en) 1998-08-13
AU739067B2 (en) 2001-10-04
GB9918007D0 (en) 1999-09-29
WO1998035047A1 (en) 1998-08-13
CN1252097A (zh) 2000-05-03
JP2002514915A (ja) 2002-05-21
GB2341863B (en) 2001-09-05

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO PUBLICADA NA RPI 1942 DE 25 - 03 - 2008.